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There are 133857 results for: content related to: A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes

  1. Commonly used tag combinations for tandem affinity purification

    Biotechnology and Applied Biochemistry

    Volume 55, Issue 2, February 2010, Pages: 73–83, Yifeng Li

    Article first published online : 23 DEC 2010, DOI: 10.1042/BA20090273

  2. You have free access to this content
    Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling

    The EMBO Journal

    Volume 31, Issue 11, May 30, 2012, Pages: 2629–2647, Michael Röring, Ricarda Herr, Gina J Fiala, Katharina Heilmann, Sandra Braun, Anja E Eisenhardt, Sebastian Halbach, David Capper, Andreas von Deimling, Wolfgang W Schamel, Darren N Saunders and Tilman Brummer

    Article first published online : 17 APR 2012, DOI: 10.1038/emboj.2012.100

  3. Affinity-purification coupled to mass spectrometry: Basic principles and strategies


    Volume 12, Issue 10, May 2012, Pages: 1576–1590, Wade H. Dunham, Michael Mullin and Anne-Claude Gingras

    Article first published online : 19 JUN 2012, DOI: 10.1002/pmic.201100523

  4. Two steps forward—one step back: Advances in affinity purification mass spectrometry of macromolecular complexes


    Volume 12, Issue 10, May 2012, Pages: 1591–1608, Marlene Oeffinger

    Article first published online : 19 JUN 2012, DOI: 10.1002/pmic.201100509

  5. Tap-Tagging System in Rice for Protein Complex Isolation

    Plant Proteomics: Technologies, Strategies, and Applications

    Jai S. Rohila, Michael E. Fromm, Pages: 525–542, 2008

    Published Online : 7 JAN 2008, DOI: 10.1002/9780470369630.ch36

  6. Strep/FLAG Tandem Affinity Purification (SF-TAP) to Study Protein Interactions


    Current Protocols in Protein Science


    Christian Johannes Gloeckner, Karsten Boldt and Marius Ueffing

    Published Online : 1 AUG 2009, DOI: 10.1002/0471140864.ps1920s57

  7. Methods for the detection and analysis of protein–protein interactions


    Volume 7, Issue 16, No. 16 August 2007, Pages: 2833–2842, Tord Berggård, Sara Linse and Peter James

    Article first published online : 19 JUL 2007, DOI: 10.1002/pmic.200700131

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    Functional characterization of a BRAF insertion mutant associated with pilocytic astrocytoma

    International Journal of Cancer

    Volume 129, Issue 9, 1 November 2011, Pages: 2297–2303, Anja E. Eisenhardt, Heike Olbrich, Michael Röring, Wibke Janzarik, Ton Nu Van Anh, Huriye Cin, Marc Remke, Hendrik Witt, Andrey Korshunov, Stefan M. Pfister, Heymut Omran and Tilman Brummer

    Article first published online : 25 JUL 2011, DOI: 10.1002/ijc.25893

  9. Proteins interacting with mitochondrial ATP-dependent Lon protease (MAP1) in Magnaporthe oryzae are involved in rice blast disease

    Molecular Plant Pathology

    Volume 16, Issue 8, October 2015, Pages: 847–859, Xiao Cui, Yi Wei, Yu-Han Wang, Jian Li, Fuk-Ling Wong, Ya-Jie Zheng, Hai Yan, Shao-Shuai Liu, Jin-Liang Liu, Bao-Lei Jia and Shi-Hong Zhang

    Article first published online : 21 MAR 2015, DOI: 10.1111/mpp.12242

  10. You have full text access to this OnlineOpen article
    An atlas of chaperone–protein interactions in Saccharomyces cerevisiae: implications to protein folding pathways in the cell

    Molecular Systems Biology

    Volume 5, Issue 1, 2009, Yunchen Gong, Yoshito Kakihara, Nevan Krogan, Jack Greenblatt, Andrew Emili, Zhaolei Zhang and Walid A Houry

    Article first published online : 16 JUN 2009, DOI: 10.1038/msb.2009.26

  11. Raf-1 and B-Raf proteins have similar regional distributions but differential subcellular localization in adult rat brain

    European Journal of Neuroscience

    Volume 11, Issue 6, June 1999, Pages: 1995–2006, Cecile Morice, Fatiha Nothias, Sandra König, Philippe Vernier, Manuela Baccarini, Jean-Didier Vincent and Jean Vianney Barnier

    Article first published online : 9 OCT 2008, DOI: 10.1046/j.1460-9568.1999.00609.x

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    Biochemical analysis of PIFTC3, the Trypanosoma brucei orthologue of nematode DYF-13, reveals interactions with established and putative intraflagellar transport components

    Molecular Microbiology

    Volume 78, Issue 1, October 2010, Pages: 173–186, Joseph B. Franklin and Elisabetta Ullu

    Article first published online : 18 AUG 2010, DOI: 10.1111/j.1365-2958.2010.07322.x

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    Activation of B-Raf kinase requires phosphorylation of the conserved residues Thr598 and Ser601

    The EMBO Journal

    Volume 19, Issue 20, October 16, 2000, Pages: 5429–5439, Bao-Hong Zhang and Kun-Liang Guan

    Article first published online : 16 OCT 2000, DOI: 10.1093/emboj/19.20.5429

  14. Mass spectrometry-based proteomic analysis of the epitope-tag affinity purified protein complexes in eukaryotes


    Volume 6, Issue 23, No. 23 December 2006, Pages: 6158–6166, Ing-Feng Chang

    Article first published online : 30 OCT 2006, DOI: 10.1002/pmic.200600225

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    Inducible gene deletion reveals different roles for B-Raf and Raf-1 in B-cell antigen receptor signalling

    The EMBO Journal

    Volume 21, Issue 21, November 1, 2002, Pages: 5611–5622, Tilman Brummer, Peter E. Shaw, Michael Reth and Yukiko Misawa

    Article first published online : 1 NOV 2002, DOI: 10.1093/emboj/cdf588

  16. You have free access to this content
    Protein–protein interactions of tandem affinity purification-tagged protein kinases in rice

    The Plant Journal

    Volume 46, Issue 1, April 2006, Pages: 1–13, Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald Cerny, Chris Dardick, Patrick Canlas, Xia Xu, Michael Gribskov, Siddhartha Kanrar, Jian-Kang Zhu, Pamela Ronald and Michael E. Fromm

    Article first published online : 28 FEB 2006, DOI: 10.1111/j.1365-313X.2006.02671.x

  17. Functional consequences of mutations in a putative Akt phosphorylation motif of B-raf in human cancers

    Molecular Carcinogenesis

    Volume 43, Issue 1, May 2005, Pages: 59–63, Tsuneo Ikenoue, Fumihiko Kanai, Yohko Hikiba, Yasuo Tanaka, Jun Imamura, Miki Ohta, Amarsanaa Jazag, Bayasi Guleng, Yoshinari Asaoka, Keisuke Tateishi, Takayuki Kawakami, Masayuki Matsumura, Takao Kawabe and Masao Omata

    Article first published online : 24 MAR 2005, DOI: 10.1002/mc.20102

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    TCR ligand avidity determines the modeof B-Raf/Raf-1/ERK activation leading to the activationof human CD4+ T cell clone

    European Journal of Immunology

    Volume 36, Issue 7, July 2006, Pages: 1926–1937, Hirotake Tsukamoto, Atsushi Irie, Yu-Zhen Chen, Kumiko Takeshita, Jeong-Ran Kim and Yasuharu Nishimura

    Article first published online : 22 JUN 2006, DOI: 10.1002/eji.200535803

  19. Uncoupling of bait-protein expression from the prey protein environment adds versatility for cell and tissue interaction proteomics and reveals a complex of CARP-1 and the PKA Cβ1 subunit


    Volume 10, Issue 16, No. 16 August 2010, Pages: 2890–2900, Andrea Erlbruch, Chien-Wen Hung, Joerg Seidler, Katrin Borrmann, Frank Gesellchen, Norbert König, Dieter Kübler, Friedrich W. Herberg, Wolf D. Lehmann and Dirk Bossemeyer

    Article first published online : 16 JUN 2010, DOI: 10.1002/pmic.200900593

  20. Optimization of Diarylthiazole B-Raf Inhibitors: Identification of a Compound Endowed with High Oral Antitumor Activity, Mitigated hERG Inhibition, and Low Paradoxical Effect


    Volume 10, Issue 2, February 2015, Pages: 276–295, Dr. Maurizio Pulici, Dr. Gabriella Traquandi, Dr. Chiara Marchionni, Dr. Michele Modugno, Dr. Rosita Lupi, Nadia Amboldi, Dr. Elena Casale, Dr. Nicoletta Colombo, Luca Corti, Dr. Marina Fasolini, Dr. Fabio Gasparri, Wilma Pastori, Dr. Alessandra Scolaro, Dr. Daniele Donati, Dr. Eduard Felder, Dr. Arturo Galvani, Dr. Antonella Isacchi, Dr. Enrico Pesenti and Dr. Marina Ciomei

    Article first published online : 27 NOV 2014, DOI: 10.1002/cmdc.201402424