Biocalorimetry 2: Applications of Calorimetry in the Biological Sciences

Biocalorimetry 2: Applications of Calorimetry in the Biological Sciences

Editor(s): John E. Ladbury, Michael L. Doyle

Published Online: 2 JUN 2005

Print ISBN: 9780470849682

Online ISBN: 9780470011126

DOI: 10.1002/0470011122

About this Book

Over the last decade, high-sensitivity calorimetry has developed from a specialist method used mainly by dedicated experts to a major, commercially available tool in the arsenal directed at understanding molecular interactions and stability. Calorimeters have now become commonplace in bioscience laboratories. As a result, the number of those proficient in experimentation in this field has risen dramatically, as has the range of experiments to which these methods have been applied. Applications extend from studies in small molecule and solvent biophysics, through drug screening to whole cell assays. The technology has developed to include higher levels of sensitivity (and hence smaller sample size requirements) and a drive towards high-throughput technology, creating a very large user base in both academia and the pharmaceutical industry.

This book is a fully revised and updated edition of the successful Biocalorimetry:Applications of Calorimetry in the Biological Sciences, published in 1998. Since then, there have been many advances in the instrumentation as well as in its applications and methodology.  There are general chapters highlighting the usage of the isothermal titration calorimeter and the differential scanning calorimeter, more advanced chapters on specific applications and tutorials that cover the idiosyncrasies of experimental methods and data analysis. The book draws these together to create the definitive biological calorimetric text book. 

This book both explains the background to the method and describes novel, high-impact applications.  It features works of interest to the experienced calorimetrist and the enthusiastic dilettante.  The book should be of interest to all working in the field of biocalorimetry, from graduate students to researchers in academia and in industry.

Table of contents

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  1. Part I: General Introduction

  2. Part II: Isothermal Titration Calorimetry

    1. Chapter 7

      Energetics of the Interaction of Human Acidic Fibroblast Growth Factor with Heparin and the Functional Analogue Myo-Inositol Hexasulfate (pages 133–150)

      Mercedes Guzmán-Casado, María M. García-Mira, Pedro Cano-Soldado, Guillermo Giménez-Gallego, Jose M. Sanchez-Ruiz and Antonio Parody-Morreale

    2. Chapter 8

      Thermodynamics of SH2 Domain Binding (pages 151–173)

      Gabriel Waksman, Sangaralingam Kumaran and Olga Lubman

  3. Part III: Differential Scanning Calorimetry

    1. Chapter 13

      Microcalorimetry as Applied to Psychrophilic Enzymes (pages 231–240)

      Salvino D'amico, Daphné Georlette, Tony Collins, Georges Feller and Charles Gerday

    2. Chapter 14

      An Autosampling Differential Scanning Calorimeter for Study of Biomolecular Interactions (pages 241–251)

      Valerian Plotnikov, Andrew Rochalski, Michael Brandts, John F. Brandts, Samuel Williston, Verna Frasca and Lung-Nan Lin

    1. You have free access to this content