Online ISBN: 9780470015902

DOI: 10.1002/047001590X

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  1. Biochemistry
    1. Biochemical and Biophysical Techniques
      1. Acquired Bleeding Disorders
      2. Affinity Chromatography
      3. Affinity Purification of Antibodies
      4. Agglutination Techniques for Detecting Antigen–Antibody Reactions
      5. Alkaline Blotting of DNA Gels
      6. Antibody Microarrays
      7. Antibody Purification by Ammonium Sulfate Precipitation
      8. Array-based Proteomics
      9. Automated Protein Production Technologies
      10. Autoradiography and Fluorography
      11. Biological Computation
      12. Biological Macromolecules: UV-visible Spectrophotometry
      13. Bioluminescence
      14. Biomolecular NMR Spectroscopy of Ribonucleic Acids
      15. Biosensors
      16. Caenorhabditis elegans as an Experimental Organism
      17. Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters
      18. Calorimetry
      19. Capillary Blotting of RNA and DNA Gels
      20. Capillary Electrophoresis
      21. Capillary Electrophoresis
      22. Catalytic Antibodies
      23. Centrifugation Techniques
      24. Chemical Ecology
      25. Chromatofocusing
      26. Chromatographic Techniques
      27. Circular Dichroism: Studies of Proteins
      28. Clinical Chemistry in Paediatrics
      29. Clinical Chemistry in Pregnancy
      30. Conjugation of Proteins with Horseradish Peroxidase (HRP)
      31. Crystallisation of Nucleic Acids
      32. Crystallisation of Proteins and Macromolecular Complexes: Past, Present and Future
      33. Denaturation of RNA Using Glyoxal
      34. Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
      35. Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels
      36. Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels
      37. You have free access to this content
        Dideoxy Sequencing of DNA
      38. Difference Gel Electrophoresis (DIGE)
      39. Direct Labelling of Proteins with 125I
      40. Direct Sequencing of PCR Products
      41. Dissimilatory Metal Transformations by Microorganisms
      42. Electroblotting of Gels
      43. Electroelution of Proteins from Polyacrylamide Gels
      44. Electron Cryomicroscopy and Three-dimensional Computer Reconstruction of Biological Molecules
      45. Electron Paramagnetic Resonance (EPR) and Spin-labelling
      46. Electrophoresis of DNA and RNA in Non-denaturing Vertical Polyacrylamide Gels
      47. EM Analysis of Protein Structure
      48. Engineered Enzymes
      49. Enzyme Activity and Assays
      50. Enzyme Labelling of Antibodies
      51. Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein
      52. Enzymes: Purification
      53. Enzymology Methods
      54. Flow Cytometers
      55. Fluorescence Recovery After Photobleaching (FRAP)
      56. Fluorescence Resonance Energy Transfer
      57. Fluorescence Spectrophotometry
      58. Fluorescent Analogues in Biological Research
      59. Fluorescent Probes Used for Measuring Intracellular Calcium
      60. Fourier Transform Infrared (FTIR) Spectroscopy
      61. Free Drug Monitoring
      62. Fusion Proteins as Research Tools
      63. Gel Electrophoresis of Proteins: High-resolution Two-dimensional
      64. Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels
      65. Gel Electrophoresis: One-dimensional
      66. Gel-Staining Techniques – Dyeing to Know It All
      67. Gene Inactivation Strategies: An Update
      68. Gene Localization by in situ Hybridization: FISH
      69. Genetic Mapping: Comparison of Direct and Indirect Approaches
      70. Genomic DNA: Purification
      71. Green Fluorescent Protein (GFP)
      72. History of Drug Discovery
      73. Homogenization of Soft Tissues (Rat Liver) in Iso-osmotic Media
      74. Hydrocarbon Metabolism
      75. Identification of Protein–Protein Interactions
      76. Image Analysis Tools in Proteomics
      77. Immunoassay
      78. Immunoelectrophoresis
      79. Immunoglobulin Purification
      80. Immunoglobulin Superfamily
      81. Immunoprecipitation Techniques
      82. Industrialization of Proteomics: Scaling UpProteomics Processes
      83. Intracellular pH Measurement
      84. Ion Exchange Chromatography
      85. Isolation of DNA from Agarose Gels using Agarase
      86. Isolation of DNA from Low Gelling Temperature Agarose
      87. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
      88. Laser Capture Microdissection in Proteomics
      89. Limits of Proteomics: Protein Solubilisation Issues
      90. Liquid Chromatography
      91. Long-range PCR
      92. Macromolecular Interactions: Aptamers
      93. Macromolecular Interactions: Light Scattering
      94. Magnetic Tweezers
      95. MALDI-Mass Spectrometric Imaging of Endogenous Metabolites in Biological Systems
      96. Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
      97. Manual Dot Blotting
      98. Mass Measurements by Scanning Transmission Electron Microscopy
      99. Mass Spectrometry: Peptide Sequencing
      100. Metabolic Turnover
      101. Method for the Concentration of RNA and DNA Samples
      102. Microarrays in Toxicological Research
      103. Microarrays: Use in Gene Identification
      104. Mitochondria Protein Import: Methods
      105. Multispot Array Technologies
      106. Muscle Contraction Mechanisms: Use of Synchrotron X-ray Diffraction
      107. Neutron Diffraction
      108. NMR Spectroscopy for Monitoring Functional Dynamics in Solution
      109. Nuclear Magnetic Resonance (NMR) Spectroscopy: Structure Determination of Proteins and Nucleic Acids
      110. Northern Blotting
      111. Nuclear Magnetic Resonance (NMR) Spectroscopy in the Study of Whole-animal Metabolism
      112. Nuclear Magnetic Resonance (NMR) Spectroscopy of Proteins
      113. Nucleic Acid Hybridization
      114. Nucleic Acids: Subtractive Hybridization
      115. Nucleolar Proteomics
      116. OD Unit
      117. PCR-mediated Mutagenesis
      118. Peptide Mapping
      119. Peptide Sequencing by Edman Degradation
      120. Permeabilised Mammalian Cell Systems for Protein Synthesis
      121. Phase Problem in X-ray Crystallography, and Its Solution
      122. Phosphoproteomics
      123. Phosphorimager
      124. Polymerase Processivity: Measurement and Mechanisms
      125. Prebiotic Chemistry
      126. Preparation Artefacts in Proteomics: Technological Issues
      127. Primer on Protein–Protein Interaction Maps
      128. Protein Characterisation in Proteomics
      129. Protein Characterization: Analytical Approaches and Applications to Proteomics
      130. Protein Denaturation and the Denatured State
      131. Protein Determination
      132. Protein Export from Endoplasmic Reticulum to the Cytosol: In Vitro Methods
      133. Protein Homology Modelling
      134. Protein Identification: Overlay Procedures
      135. Protein Import into Peroxisomes: The Principles and Methods of Studying
      136. Protein Kinases in the Era of Precision Medicine
      137. Protein Quantitation: Bradford Method
      138. Protein Quantitation: Lowry Protocol
      139. Protein Sorting During Endocytosis: Methods
      140. Protein Stability
      141. Protein Structure Design and Engineering
      142. Protein Synthesis: Measuring Errors
      143. Protein–DNA Interactions: Techniques Used
      144. Proteins: Affinity Tags
      145. Pulsed-field Gel Electrophoresis
      146. Pulsed-field Gel Electrophoresis of DNA
      147. Purifying and Analysing Cytosolic Protein Complexes
      148. Quantification of DNA and RNA: a Quantitative Visual Comparison
      149. Quantification of DNA and RNA: A Spectrophotometric Method
      150. Quantitative Proteomics (ICAT™)
      151. Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
      152. Receptor Binding in Drug Discovery
      153. Recovery of DNA from Polyacrylamide or Agarose by Electroelution
      154. Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
      155. Sedimentation
      156. Separation of RNA and DNA in Methylmercuric Hydroxide Denaturing Agarose Gels
      157. Sequence Complexity and Composition
      158. Shotgun Proteomics
      159. Single-Step Method of Total RNA Isolation by Guanidine–Phenol Extraction
      160. Site-directed Mutagenesis Using PCR
      161. Size-exclusion Chromatography
      162. Solid-phase Peptide Synthesis: Fmoc
      163. Spectroscopic Techniques
      164. Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes
      165. Structural Proteomics: Large-Scale Studies
      166. Surface Plasmon Resonance
      167. Tandem Affinity Purification (TAP) Tags
      168. Time-resolved X-ray Crystallography
      169. Titration and pH Measurement
      170. Transcriptomics and Proteomics: Integration?
      171. Tricks and Tracks in the Identification and Quantification of Endocannabinoids
      172. Two-dimensional Electron Crystallography
      173. Two-dimensional Gel Electrophoresis
      174. Ultracentrifugation
      175. X-ray Absorption Spectroscopy
      176. X-ray Diffraction at Synchrotron Light Sources
      177. X-ray Diffraction: Principles
    2. Biochemistry of Cell Motility
    3. Bioenergetics
    4. Biomolecular Interactions
    5. Cell Signalling
    6. Cell Structure
    7. Enzymes: Structure and Action Mechanism
    8. General Biochemistry
    9. Information Flow
    10. Lipids: Structure, Function, Metabolism
    11. Molecular Transport
    12. Nucleic Acids: Structure, Function, Metabolism
    13. Other Biomolecules: Structure, Function, Metabolism
    14. Proteins: Structure, Function, Metabolism
  2. Bioethics & Philosophy
  3. Cell Biology
  4. Developmental Biology
  5. Ecology
  6. Evolution & Diversity of Life
  7. Genetics & Disease
  8. Immunology
  9. Microbiology
  10. Molecular Biology
  11. Neuroscience
  12. Plant Science
  13. Science & Society
  14. Structural Biology
  15. Virology