eLS

eLS

Online ISBN: 9780470015902

DOI: 10.1002/047001590X

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  1. Biochemistry
    1. Biochemical and Biophysical Techniques
      1. You have free access to this content
        A Biophysical Toolkit for Molecular Interactions
      2. Acquired Bleeding Disorders
      3. Affinity Chromatography
      4. Affinity Purification of Antibodies
      5. Agglutination Techniques for Detecting Antigen–Antibody Reactions
      6. Alkaline Blotting of DNA Gels
      7. Antibody Microarrays
      8. Antibody Purification by Ammonium Sulfate Precipitation
      9. Array-based Proteomics
      10. Automated Protein Production Technologies
      11. Autoradiography and Fluorography
      12. Biological Computation
      13. Biological Macromolecules: UV-visible Spectrophotometry
      14. Bioluminescence
      15. Biomolecular NMR Spectroscopy of Ribonucleic Acids
      16. Biosensors
      17. Caenorhabditis elegans as an Experimental Organism
      18. Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters
      19. Calorimetry
      20. Capillary Blotting of RNA and DNA Gels
      21. Capillary Electrophoresis
      22. Capillary Electrophoresis
      23. Catalytic Antibodies
      24. Centrifugation Techniques
      25. Chemical Ecology
      26. Chromatofocusing
      27. Chromatographic Techniques
      28. Circular Dichroism: Studies of Proteins
      29. Clinical Chemistry in Paediatrics
      30. Clinical Chemistry in Pregnancy
      31. Conjugation of Proteins with Horseradish Peroxidase (HRP)
      32. Crystallisation of Nucleic Acids
      33. Crystallisation of Proteins and Macromolecular Complexes: Past, Present and Future
      34. Denaturation of RNA Using Glyoxal
      35. Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
      36. Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels
      37. Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels
      38. You have free access to this content
        Dideoxy Sequencing of DNA
      39. Difference Gel Electrophoresis (DIGE)
      40. Direct Labelling of Proteins with 125I
      41. Direct Sequencing of PCR Products
      42. Dissimilatory Metal Transformations by Microorganisms
      43. Electroblotting of Gels
      44. Electroelution of Proteins from Polyacrylamide Gels
      45. Electron Cryomicroscopy and Three-dimensional Computer Reconstruction of Biological Molecules
      46. Electron Paramagnetic Resonance (EPR) and Spin-labelling
      47. Electrophoresis of DNA and RNA in Non-denaturing Vertical Polyacrylamide Gels
      48. EM Analysis of Protein Structure
      49. Engineered Enzymes
      50. Enzyme Activity and Assays
      51. Enzyme Labelling of Antibodies
      52. Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein
      53. Enzymes: Purification
      54. Enzymology Methods
      55. Flow Cytometers
      56. Fluorescence Recovery After Photobleaching (FRAP)
      57. Fluorescence Resonance Energy Transfer
      58. Fluorescence Spectrophotometry
      59. Fluorescent Analogues in Biological Research
      60. Fluorescent Probes Used for Measuring Intracellular Calcium
      61. Fourier Transform Infrared (FTIR) Spectroscopy
      62. Free Drug Monitoring
      63. Fusion Proteins as Research Tools
      64. Gel Electrophoresis of Proteins: High-resolution Two-dimensional
      65. Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels
      66. Gel Electrophoresis: One-dimensional
      67. Gel-Staining Techniques – Dyeing to Know It All
      68. Gene Inactivation Strategies: An Update
      69. Gene Localization by in situ Hybridization: FISH
      70. Genetic Mapping: Comparison of Direct and Indirect Approaches
      71. Genomic DNA: Purification
      72. Green Fluorescent Protein (GFP)
      73. History of Drug Discovery
      74. Homogenization of Soft Tissues (Rat Liver) in Iso-osmotic Media
      75. Hydrocarbon Metabolism
      76. Hydrophobic Interaction Chromatography
      77. Identification of Protein–Protein Interactions
      78. Image Analysis Tools in Proteomics
      79. Immunoassay
      80. Immunoelectrophoresis
      81. Immunoglobulin Purification
      82. Immunoglobulin Superfamily
      83. Immunoprecipitation Techniques
      84. Industrialization of Proteomics: Scaling UpProteomics Processes
      85. Intracellular pH Measurement
      86. Ion Exchange Chromatography
      87. Isolation of DNA from Agarose Gels using Agarase
      88. Isolation of DNA from Low Gelling Temperature Agarose
      89. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
      90. Laser Capture Microdissection in Proteomics
      91. Limits of Proteomics: Protein Solubilisation Issues
      92. Liquid Chromatography
      93. Long-range PCR
      94. Macromolecular Interactions: Aptamers
      95. Macromolecular Interactions: Light Scattering
      96. Magnetic Tweezers
      97. MALDI-Mass Spectrometric Imaging of Endogenous Metabolites in Biological Systems
      98. Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
      99. Manual Dot Blotting
      100. Mass Measurements by Scanning Transmission Electron Microscopy
      101. Mass Spectrometry: Peptide Sequencing
      102. Metabolic Turnover
      103. Method for the Concentration of RNA and DNA Samples
      104. Microarrays in Toxicological Research
      105. Microarrays: Use in Gene Identification
      106. Mitochondria Protein Import: Methods
      107. Multispot Array Technologies
      108. Muscle Contraction Mechanisms: Use of Synchrotron X-ray Diffraction
      109. Neutron Diffraction
      110. NMR Spectroscopy for Monitoring Functional Dynamics in Solution
      111. Northern Blotting
      112. Nuclear Magnetic Resonance (NMR) Spectroscopy in the Study of Whole-animal Metabolism
      113. Nuclear Magnetic Resonance (NMR) Spectroscopy of Proteins
      114. Nucleic Acid Hybridization
      115. Nucleic Acids: Subtractive Hybridization
      116. Nucleolar Proteomics
      117. OD Unit
      118. PCR-mediated Mutagenesis
      119. Peptide Mapping
      120. Peptide Sequencing by Edman Degradation
      121. Permeabilised Mammalian Cell Systems for Protein Synthesis
      122. Phase Problem in X-ray Crystallography, and Its Solution
      123. Phosphoproteomics
      124. Phosphorimager
      125. Polymerase Processivity: Measurement and Mechanisms
      126. Prebiotic Chemistry
      127. Preparation Artefacts in Proteomics: Technological Issues
      128. Primer on Protein–Protein Interaction Maps
      129. Protein Characterisation in Proteomics
      130. Protein Characterization: Analytical Approaches and Applications to Proteomics
      131. Protein Denaturation and the Denatured State
      132. Protein Determination
      133. Protein Export from Endoplasmic Reticulum to the Cytosol: In Vitro Methods
      134. Protein Homology Modelling
      135. Protein Identification: Overlay Procedures
      136. Protein Import into Peroxisomes: The Principles and Methods of Studying
      137. Protein Kinases in the Era of Precision Medicine
      138. Protein Quantitation: Bradford Method
      139. Protein Quantitation: Lowry Protocol
      140. Protein Sorting During Endocytosis: Methods
      141. Protein Stability
      142. Protein Structure Design and Engineering
      143. Protein Synthesis: Measuring Errors
      144. Protein–DNA Interactions: Techniques Used
      145. Proteins: Affinity Tags
      146. Pulsed-field Gel Electrophoresis
      147. Pulsed-field Gel Electrophoresis of DNA
      148. Purifying and Analysing Cytosolic Protein Complexes
      149. Quantification of DNA and RNA: a Quantitative Visual Comparison
      150. Quantification of DNA and RNA: A Spectrophotometric Method
      151. Quantitative Proteomics (ICAT™)
      152. Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
      153. Receptor Binding in Drug Discovery
      154. Recovery of DNA from Polyacrylamide or Agarose by Electroelution
      155. Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
      156. Sedimentation
      157. Separation of RNA and DNA in Methylmercuric Hydroxide Denaturing Agarose Gels
      158. Sequence Complexity and Composition
      159. Shotgun Proteomics
      160. Single-Step Method of Total RNA Isolation by Guanidine–Phenol Extraction
      161. Site-directed Mutagenesis Using PCR
      162. Size-exclusion Chromatography
      163. Solid-phase Peptide Synthesis: Fmoc
      164. Spectroscopic Techniques
      165. Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes
      166. Structural Proteomics: Large-Scale Studies
      167. Surface Plasmon Resonance
      168. Tandem Affinity Purification (TAP) Tags
      169. Time-resolved X-ray Crystallography
      170. Titration and pH Measurement
      171. Transcriptomics and Proteomics: Integration?
      172. Tricks and Tracks in the Identification and Quantification of Endocannabinoids
      173. Two-dimensional Electron Crystallography
      174. Two-dimensional Gel Electrophoresis
      175. Ultracentrifugation
      176. X-ray Absorption Spectroscopy
      177. X-ray Diffraction at Synchrotron Light Sources
      178. X-ray Diffraction: Principles
    2. Biochemistry of Cell Motility
    3. Bioenergetics
    4. Biomolecular Interactions
    5. Cell Signalling
    6. Cell Structure
    7. Enzymes: Structure and Action Mechanism
    8. General Biochemistry
    9. Information Flow
    10. Lipids: Structure, Function, Metabolism
    11. Molecular Transport
    12. Nucleic Acids: Structure, Function, Metabolism
    13. Other Biomolecules: Structure, Function, Metabolism
    14. Proteins: Structure, Function, Metabolism
  2. Bioethics & Philosophy
  3. Cell Biology
  4. Developmental Biology
  5. Ecology
  6. Evolution & Diversity of Life
  7. Genetics & Disease
  8. Immunology
  9. Microbiology
  10. Molecular Biology
  11. Neuroscience
  12. Plant Science
  13. Science & Society
  14. Structural Biology
  15. Virology

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