Online ISBN: 9780470015902

DOI: 10.1002/047001590X

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  1. Biochemistry
  2. Bioethics & Philosophy
  3. Cell Biology
    1. Basic Cell Biology, Protein, RNA and DNA
    2. Cell Biology of Cancer
    3. Cell Biology of Organogenesis
    4. Cell Compartments and Cellular Organelles
    5. Cell Cycle
    6. Cell Cytoskeleton
    7. Cell Death and Cellular Senescence
    8. Cell Energetics
    9. Cell Membranes
    10. Cell Motility
    11. Cell Shape
    12. Cellular Transport
    13. Chromosome Segregation
    14. General Cell Biology
    15. Growth and Synthesis
    16. Intracellular and Extracellular Signalling
    17. Multicellularity
    18. Techniques in Cell Biology
      1. Acanthamoeba castellanii Cell Culture
      2. Advanced Techniques for Cell Lineage Labelling in Drosophila
      3. Affinity Purification of Antibodies
      4. Animal Cell Culture Scale-up
      5. Animal Cell Separation and Subcellular Fractionation
      6. Antibody Purification by Ammonium Sulfate Precipitation
      7. Apoptosis: Morphological Criteria and Other Assays
      8. Array-based Proteomics
      9. Atomic Force Microscopy
      10. Automated Protein Production Technologies
      11. Banding Techniques
      12. Biosensors
      13. Brain Organoids
      14. Caenorhabditis elegans Culture
      15. CD Antigens
      16. Cell Culture Contamination
      17. Cell Culture Media
      18. Cell Culture: Basic Procedures
      19. Cell Cycle Analysis by Flow Cytometry
      20. Cell Cycle: Synchronization at Various Stages
      21. Cell Invasion Assays
      22. Cell Line Authentication and Characterisation
      23. Cell Proliferation Assays
      24. Cell Senescence In Vitro
      25. Cell Separation Techniques Used in Immunology
      26. Cell Staining: Fluorescence imaging of mitochondrial structure and function
      27. Cell Staining: Fluorescent Labelling of Endocytic Compartments
      28. Cell Staining: Fluorescent Labelling of the Endoplasmic Reticulum
      29. Cell Staining: Fluorescent Labelling of the Golgi Apparatus
      30. Centrosomes: Methods for Preparation
      31. Chromosome
      32. Chromosome Analysis and Identification
      33. Chromosome Preparation
      34. You have free access to this content
        Chromosome Preparation and Banding
      35. Chromosome Structures: Visualization
      36. Chromosomes: Methods for Preparation
      37. Clathrin-coated Vesicles: Methods for Preparation
      38. Clonal Isolation and Plating Efficiency
      39. Confocal Microscopy
      40. Conjugation of Proteins with Horseradish Peroxidase (HRP)
      41. Correlative Light and Electron Microscopy: Methods and Applications
      42. Cotranslational Translocation of Proteins Into Microsomes: Methods
      43. Cryogenic Detectors: Detection of Single Molecules
      44. Cryopreservation and Thawing of Mammalian Cells
      45. Cytogenetic Techniques
      46. Cytotoxicity Assays
      47. Deconvolution Methods for Low Exposure Fluorescence Microscopy
      48. Dictyostelium as a Biomedical Model
      49. Dictyostelium discoideum: Cell Culture and Molecular Tools
      50. Difference Gel Electrophoresis (DIGE)
      51. Differentiation of Somatic Cells, Stem Cells and Stem Cell Variants: In Vitro Models
      52. Digital Image Analysis
      53. Direct Labelling of Proteins with 125I
      54. Dynamic Clamp
      55. Ectopic Transcription
      56. Electron Microscopy
      57. Electron Tomography
      58. Electroporation
      59. EM Analysis of Protein Structure
      60. Endocytic and Exocytic Transport Assays
      61. Endocytic Organelles: Methods for Preparation and Analysis
      62. Endoplasmic Reticulum to Golgi Transport: Methods
      63. Enzyme Labelling of Antibodies
      64. Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein
      65. Expression Analysis In Vivo
      66. Expression Analysis In vivo: Cell Systems
      67. Expression Tags for Protein Production
      68. Far-field Light Microscopy
      69. Flow-sorted Chromosomes
      70. Fluorescence In Situ Hybridization (FISH) Techniques
      71. Fluorescence Microscopy
      72. Fluorescence Microscopy
      73. Fluorescence Recovery After Photobleaching (FRAP)
      74. Fluorescent Labelling of Antibodies
      75. Fluorescent Probes Used for Measuring Intracellular Calcium
      76. Golgi: Methods for Preparation
      77. High-throughput ‘On Chip’ Protein and Nucleic Acid Transfection
      78. Histochemical Staining
      79. Histocultures and Their Use
      80. History of the Electron Microscope in Cell Biology
      81. Homogenization of Tissue Culture Cells in Suspension
      82. Hydrophobic Interaction Chromatography
      83. Image Analysis Tools in Proteomics
      84. Immunization of Mice for Monoclonal Antibody Production
      85. Immunodiffusion
      86. Immunofluorescence
      87. Immunofluorescence: Protocol
      88. Immunological Cytotoxicity Studies: in vitro
      89. Impedimetric Monitoring of Cell-Based Assays
      90. In Situ Hybridization
      91. In Vitro Mutagenesis
      92. Indirect Immunofluorescence of Cultured Cells
      93. Indirect Immunofluorescence of Tissues
      94. Industrialization of Proteomics: Scaling UpProteomics Processes
      95. Insect Cell Culture
      96. Interference Reflection Microscopy
      97. Intracellular pH Measurement
      98. Isolation of a Light Mitochondrial Fraction from a Homogenate
      99. Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient
      100. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
      101. Isolation of Plasmid DNA from E. coli
      102. Karyotype Analysis and Chromosome Banding
      103. Karyotype Interpretation
      104. Labelling of Cells Engaged in DNA Synthesis: Autoradiography and BrdU Staining
      105. Laser Capture Microdissection in Proteomics
      106. Light Microscopy
      107. Light Microscopy Imaging Facilities
      108. Limits of Proteomics: Protein Solubilisation Issues
      109. You have free access to this content
      110. Lymphocyte Responses In Vitro
      111. Mass Spectrometry Instrumentation in Proteomics
      112. Membrane Targeting: Methods
      113. Methods for Live Microscopy of Drosophila Spermatocytes
      114. Microinjection into Xenopus Oocytes
      115. Microinjection of mRNA into Somatic Cells
      116. Microprojectile Bombardment
      117. Mitochondria Protein Import: Methods
      118. Mitochondria: Methods for Preparation
      119. Modelling Plant Cell Growth
      120. Molecular Entry Point: Strategies in Proteomics
      121. Motility Assays for Microtubule Motor Proteins
      122. Mouse Knockouts: Modifying the Mouse Genome by using Embryonic Stem Cells
      123. Nuclear Magnetic Resonance (NMR) Spectroscopy in the Study of Whole-animal Metabolism
      124. Nuclear Matrix: Methods of Preparation
      125. Nuclear Pores: Methods for Preparation
      126. Nuclear Protein Import: Methods
      127. Nuclear Run-on Assays
      128. Nuclear Transfer from Cell Lines
      129. Nucleolar Proteomics
      130. Nucleus and Nuclear Envelope: Methods for Preparation
      131. Optical Tweezers
      132. Patch Fluorometry
      133. Pattern Searches in Protein Sequences
      134. Permeabilised Mammalian Cell Systems for Protein Synthesis
      135. Peroxisomes: Methods for Preparation
      136. Phagosomes: Methods for Preparation
      137. Phase Contrast Microscopy
      138. Phosphoproteomics
      139. Physarum Cell Culture
      140. Plasma Membranes: Methods for Preparation
      141. Preparation Artefacts in Proteomics: Technological Issues
      142. Preparation of Nuclei from a Homogenate using a Sucrose Barrier
      143. Preparation of Nuclei from Rat Liver using Sucrose
      144. Primary Cell Cultures and Immortal Cell Lines
      145. Primer on Protein–Protein Interaction Maps
      146. Production of Monoclonal Antibodies
      147. Production of Polyclonal Antibodies in Rabbits
      148. Promoter Fusions to Study Gene Expression
      149. Proteasomes: Methods for Preparation
      150. Protein Characterisation in Proteomics
      151. Protein Characterization: Analytical Approaches and Applications to Proteomics
      152. Protein Determination
      153. Protein Export from Endoplasmic Reticulum to the Cytosol: In Vitro Methods
      154. Protein Import into Peroxisomes: The Principles and Methods of Studying
      155. Protein Import into the Yeast Endoplasmic Reticulum: Methods
      156. Protein Production for Biotechnology
      157. Protein Production in Mammalian Cells
      158. Protein Quantitation: Bradford Method
      159. Protein Quantitation: Lowry Protocol
      160. Protein Sorting During Endocytosis: Methods
      161. Purifying and Analysing Cytosolic Protein Complexes
      162. Quantification of DNA and RNA: a Quantitative Visual Comparison
      163. Quantification of DNA and RNA: A Spectrophotometric Method
      164. Quantitative Proteomics (ICAT™)
      165. Radiation Hybrid Mapping
      166. Radioactive Labelling of Antibodies
      167. Ribosome Profiling: Principles and Variations
      168. Ribosomes: Methods for Preparation
      169. Safety Considerations in the Tissue Culture Laboratory
      170. Secretory Granules: Methods for Preparation
      171. Shotgun Proteomics
      172. Single Particle EM
      173. Site-directed Mutagenesis of Double-stranded Plasmids
      174. Somatic Cell Genetics
      175. Superresolution Microscopy
      176. Synaptic Vesicles: Methods for Preparation
      177. Synthetic Biology: Molecular Tools for Engineering Organisms
      178. Tetrahymena Cell Culture
      179. Tip Cells in Angiogenesis
      180. TIRF (Total Internal Reflection Fluorescence)
      181. Transgenic Zebrafish Production
      182. Transposon-Based Cellular Reprogramming to Induced Pluripotency
      183. Tumorigenicity Assays
      184. Two-dimensional Gel Electrophoresis
      185. Ultracentrifugation
      186. Use of Animal Cells and Display Methods for the Production of Human Monoclonal Antibodies
      187. Vesicle Transport Assay
      188. Video Microscopy
      189. Western Blotting Analysis of Translation Products
      190. Yeast Cell Culture
  4. Developmental Biology
  5. Ecology
  6. Evolution & Diversity of Life
  7. Genetics & Disease
  8. Immunology
  9. Microbiology
  10. Molecular Biology
  11. Neuroscience
  12. Plant Science
  13. Science & Society
  14. Structural Biology
  15. Virology