eLS

eLS

Online ISBN: 9780470015902

DOI: 10.1002/047001590X

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  1. Biochemistry
  2. Bioethics & Philosophy
  3. Cell Biology
  4. Developmental Biology
  5. Ecology
  6. Evolution & Diversity of Life
  7. Genetics & Disease
  8. Immunology
  9. Microbiology
  10. Molecular Biology
    1. Chromosomes and Chromatin Modifications
    2. Computational Molecular Biology
    3. DNA Damage and Repair
    4. DNA Structure and function
    5. Evolution of Coding and Functional Modules
    6. General Molecular Biology
    7. Genetic Code
    8. Mechanisms of Cell Death, Senescence and Metabolism
    9. Nonchromosomal DNA
    10. Noncoding RNA
    11. Posttranscriptional Modification
    12. Posttranslational Modification
    13. Protein Degradation
    14. RNA Structure and Function
    15. Techniques and Tools in Molecular Biology
      1. Agrobacterium tumefaciens-mediated Transformation of Plant Cells
      2. Alignment: Statistical Significance
      3. Alkaline Blotting of DNA Gels
      4. Alternative Promoter Usage
      5. Ancient DNA: Recovery and Analysis
      6. Ancient Human DNA: Phylogenetic Applications
      7. Animal Cell Separation and Subcellular Fractionation
      8. Animal Models
      9. Antisense and Ribozymes
      10. Antisense Nucleic Acids in Biotechnology
      11. Application of Chromosome Conformation Capture (3C) to the Study of Human Genetic Disease
      12. Arabidopsis thaliana as an Experimental Organism
      13. Artificial Chromosomes
      14. Artificial Chromosomes
      15. Autoradiography and Fluorography
      16. Bacillus subtilis as a Model for Bacterial Systems Biology
      17. Bacteriophage Display of Combinatorial Antibody Libraries
      18. Baculovirus Expression Strategies for Protein Complex Production
      19. Baculovirus Insect Cell Expression System
      20. Banding Techniques
      21. Barcoding South American Birds
      22. Beyond the Genome
      23. Bioinformatics
      24. You have free access to this content
        Bioinformatics
      25. Bioinformatics in Genome Sequencing Projects
      26. Biomolecular Sequence Analysis: Pattern Acquisition and Frequency Counts
      27. Blocks of Limited Haplotype Diversity
      28. Breast Cancer
      29. Caenorhabditis elegans Genome Project
      30. Calibration of a Transilluminator for UV Fixation of DNA to Nylon Filters
      31. Cancer Genome Anatomy Project
      32. Capillary Blotting of RNA and DNA Gels
      33. Capillary Electrophoresis
      34. Cell Proliferation Assays
      35. Chimera-directed Gene Repair
      36. Chromatographic Techniques
      37. Chromosome 22: Sequencing
      38. Chromosome Analysis and Identification
      39. Chromosome Preparation
      40. You have free access to this content
        Chromosome Preparation and Banding
      41. Chromosome Structures: Visualization
      42. Chromosomes in Mammals: Diversity and Evolution
      43. Chromosomes: Methods for Preparation
      44. Clinical Molecular Cytogenetics
      45. Comparative Chromosome Mapping: Rodent Models
      46. Comparative Cytogenetics Technologies
      47. Comparative Genomic Hybridization
      48. Comparing the Human and Canine Genomes
      49. Computational Methods in SNP Analysis
      50. Conditional Lethal Mutations as Experimental Tools
      51. Contig Assembly
      52. Cot Analysis: Single-copy versus Repetitive DNA
      53. Cotranslational Translocation of Proteins Into Microsomes: Methods
      54. Cre–lox-Inducible Gene Targeting
      55. CRISPR-Cas Systems for the Study of Immune Function
      56. CRISPR-Cas9: A New Tool for Gene Therapy
      57. Cytogenetic and Physical Chromosomal Maps: Integration
      58. Cytogenetic Techniques
      59. Death Receptors at the Molecular Level: Therapeutic Implications
      60. Denaturation of RNA Using Glyoxal
      61. Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
      62. Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels
      63. Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels
      64. Dictyostelium discoideum: Cell Culture and Molecular Tools
      65. You have free access to this content
        Dideoxy Sequencing of DNA
      66. Direct Labelling of Proteins with 125I
      67. Direct Sequencing of PCR Products
      68. DNA Chip Revolution
      69. You have free access to this content
        DNA Chips and Microarrays
      70. DNA Cloning
      71. DNA Evidence: Inferring Identity
      72. DNA Interstrand Crosslink Repair
      73. You have free access to this content
        DNA Profiling in Forensic Science
      74. DNA Sequence Analysis
      75. DNA: Mechanical Breakage
      76. DNA: Methods for Preparation
      77. Drosophila melanogaster Germ-line Transformation
      78. Dynamic Programming
      79. Ectopic Transcription
      80. Electroblotting of Gels
      81. Electrophoresis and Blotting of DNA
      82. Electrophoresis of DNA and RNA in Non-denaturing Vertical Polyacrylamide Gels
      83. Electroporation
      84. Engineered Enzymes
      85. Entrez and Forward Database Searching
      86. Escherichia coli and the Development of Bacterial Genetics
      87. Escherichia coli as an Experimental Organism
      88. Evolutionary Distance: Estimation
      89. You have free access to this content
        Experimental Organisms Used in Genetics
      90. Expressed-sequence Tag (EST)
      91. Expression Analysis In Vitro
      92. Expression Analysis In Vivo
      93. Expression Analysis In vivo: Cell Systems
      94. Expression Studies
      95. Expression Tags for Protein Production
      96. Filamentous Phage Display
      97. Fluorescence in situ Hybridization
      98. Fluorescence In Situ Hybridization (FISH) Techniques
      99. Fluorescent Analogues in Biological Research
      100. Forensic Genetics
      101. Forensic Genetics: Mathematics
      102. Fragile Sites
      103. Framework Map
      104. Functional Complementation
      105. Fusion Proteins as Research Tools
      106. Gel Electrophoresis
      107. Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels
      108. Gene Delivery by Viruses
      109. Gene Expression Databases
      110. Gene Expression in Escherichia coli
      111. Gene Expression in Plants
      112. Gene Expression in Yeast
      113. Gene Expression Networks
      114. Gene Families
      115. Gene Feature Identification
      116. Gene Inactivation Strategies: An Update
      117. Gene Localization by in situ Hybridization: FISH
      118. Gene Mapping and Positional Cloning
      119. Gene Mapping: Comparative
      120. Gene Synthesis for Protein Production
      121. Gene Targeting by Homologous Recombination
      122. Gene Therapy: Technology
      123. Gene Transfer and Expression in Tissue Culture Cells
      124. Generation of Mouse Models of Cancer Using Transposon-Mediated Approaches
      125. Genetic and Physical Map Correlation
      126. Genetic Databases
      127. Genetic Databases: Mining
      128. Genetic Engineering: Reporter Genes
      129. Genetic Linkage Mapping
      130. Genetic Mapping: Comparison of Direct and Indirect Approaches
      131. Genetic Maps: Direct Meiotic Analysis
      132. Genetic Maps: Integration
      133. Genetic Screening and Testing
      134. Genome Databases
      135. Genome Inserts
      136. Genome Map
      137. Genome Map: Resolution
      138. Genome Mapping
      139. Genome Mapping
      140. Genome Sequencing
      141. Genomic DNA: Purification
      142. Genomics, Pharmacogenetics and Proteomics: An Integration
      143. Gibbs Sampling and Bayesian Inference
      144. Global Alignment
      145. Hammerhead Ribozymes
      146. HAPPY Mapping
      147. Hidden Markov Models
      148. hnRNP: Methods for Preparation
      149. Homogenization of Soft Tissues (Rat Liver) in Iso-osmotic Media
      150. Human Chromosome Evolution
      151. Human Developmental Molecular Genetics
      152. You have free access to this content
        Human Gene Therapy
      153. Human Genetics: Principles
      154. Human Genome: Draft Sequence
      155. Human Variation Databases
      156. Hydrophobic Interaction Chromatography
      157. Identification of Disease Genes by CGH Microarrays
      158. Image Analysis Tools in Proteomics
      159. In Situ Hybridization
      160. In Vitro Mutagenesis
      161. Information Theories in Molecular Biology and Genomics
      162. Isolation of DNA from Agarose Gels using Agarase
      163. Isolation of DNA from Low Gelling Temperature Agarose
      164. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
      165. Isolation of Plasmid DNA from E. coli
      166. Karyotype Analysis and Chromosome Banding
      167. Karyotype Interpretation
      168. Knockout and Knock-in Animals
      169. Ligation: Theory and Practice
      170. Linkage and Association Studies: Replication
      171. Linkage and Crossing over
      172. Long-range PCR
      173. Macromolecular Interactions: Aptamers
      174. Magnetic Tweezers
      175. Mammalian Artificial Chromosomes (MACs)
      176. Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
      177. Manual Dot Blotting
      178. Mapping Gene Function in the Embryo
      179. Mass Spectrometry in Biology
      180. Mass Spectrometry Instrumentation in Proteomics
      181. The Meselson–Stahl Experiment
      182. Metagenomics and Microbial Communities
      183. Method for the Concentration of RNA and DNA Samples
      184. Mice as Experimental Organisms
      185. Microarray Bioinformatics
      186. Microarrays and Expression Profiling in Cancer
      187. Microarrays and Single Nucleotide Polymorphism (SNP) Genotyping
      188. Microarrays in Disease Diagnosis and Prognosis
      189. Microarrays in Drug Discovery and Development
      190. Microarrays in Toxicological Research
      191. Microarrays: Use in Gene Identification
      192. Microarrays: Use in Mutation Detection
      193. Microinjection into Xenopus Oocytes
      194. Microinjection of mRNA into Somatic Cells
      195. You have free access to this content
        Microorganisms: Applications in Molecular Biology
      196. Microprojectile Bombardment
      197. Microsatellites in Historic and Ancient DNA
      198. Minigenes
      199. Mining Biological Databases
      200. Minisatellites
      201. Molecular Cytogenetic Analysis: Applications in Cancer
      202. Molecular Entry Point: Strategies in Proteomics
      203. Molecular Evolution: Techniques
      204. Mouse Genetics as a Research Tool
      205. Mouse Knockouts: Modifying the Mouse Genome by using Embryonic Stem Cells
      206. Mouse N-ethyl-N-nitrosourea (ENU) Mutagenesis
      207. Multiple Alignment
      208. Multispot Array Technologies
      209. Mutagenesis: Site-Specific
      210. Mutation Detection
      211. Mutation Nomenclature
      212. Mutation Rates: Data
      213. Neurospora Genetics
      214. New Vectors for Gene Delivery: Human and Mouse Artificial Chromosomes
      215. Next Generation Sequencing Technologies and Their Applications
      216. Nick Translation
      217. Northern Blotting
      218. Northern Blotting and RNA Detection
      219. Nuclear Run-on Assays
      220. Nuclear Transfer from Cell Lines
      221. Nuclear-encoded Protein Import into Chloroplasts: Methods
      222. Nucleic Acid Hybridization
      223. Nucleic Acids: Hybridisation
      224. Nucleic Acids: Subtractive Hybridization
      225. Nucleotide Excision Repair in Bacteria
      226. Nucleotide Sequence Databases
      227. OD Unit
      228. Optical Mapping
      229. Patenting Genes and their Products
      230. Patenting of Genes: Discoveries or Inventions?
      231. PCR-mediated Mutagenesis
      232. The Peopling of the Americas as Revealed by Molecular Genetic Studies
      233. Peptide Mapping
      234. Permeabilised Mammalian Cell Systems for Protein Synthesis
      235. Phage Display Technologies
      236. Phylogenetic Footprinting
      237. Physical Maps of Chromosomes
      238. Plant Genetic Mapping Techniques
      239. You have free access to this content
        Polymerase Chain Reaction (PCR)
      240. Polymerase Chain Reaction (PCR)
      241. Polymerase Chain Reaction (PCR): Design and Optimization of Reactions
      242. Polymerase Chain Reaction (PCR): Specialised Applications
      243. You have free access to this content
        Polytene Chromosomes
      244. Primary Protein and Nucleic Acid Three-dimensional Structure Databases
      245. Primer Walking
      246. Profile Searching
      247. Promoter Fusions to Study Gene Expression
      248. Promoter Haplotypes and Gene Expression
      249. Protein Design
      250. Protein Domain Fusion
      251. Protein Production for Biotechnology
      252. Protein Production in Mammalian Cells
      253. Protein Synthesis: Measuring Errors
      254. Protein–Protein Interactions and Genetic Disease
      255. Protein–DNA Interactions: Techniques Used
      256. Proteomics and Genomics Technology: Comparison in Heart Failure and Apoptosis
      257. Proteomics: A Molecular Scanner in Medicine
      258. Pulsed-field Gel Electrophoresis
      259. Pulsed-field Gel Electrophoresis of DNA
      260. Quantification of DNA and RNA: a Quantitative Visual Comparison
      261. Quantification of DNA and RNA: A Spectrophotometric Method
      262. Radiation Hybrid Mapping
      263. Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
      264. Recombinant DNA
      265. Reconstructing Ancient DNA Sequences in Silico
      266. Recovery of DNA from Polyacrylamide or Agarose by Electroelution
      267. Representational Difference Analysis
      268. Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
      269. Restriction Enzymes
      270. Restriction Enzymes
      271. Restriction Fragment Length Polymorphism (RFLP)
      272. Restriction Mapping
      273. Restriction Mapping of Cloned DNA
      274. Reverse Transcription and PCR
      275. RNA Gene Prediction
      276. RNA Secondary Structure Prediction
      277. RNA Tertiary Structure Prediction: Computational Techniques
      278. RNA: Methods for Preparation
      279. Robotics and Automation in Molecular Genetics
      280. Saccharomyces cerevisiae: Applications
      281. Semliki Forest Virus Expression System
      282. Separation of RNA and DNA in Methylmercuric Hydroxide Denaturing Agarose Gels
      283. Sequence Accuracy and Verification
      284. Sequence Alignment
      285. Sequence Assembly
      286. Sequence Complexity and Composition
      287. Sequence Finishing
      288. Sequence Finishing on New Platforms
      289. Sequence Similarity
      290. Sequence-tagged Site (STS)
      291. Shotgunning the Human Genome: A Personal View
      292. Similarity Search
      293. Simple Repeats
      294. Single Nucleotide Polymorphisms (SNPs): Identification and Scoring
      295. Single-Step Method of Total RNA Isolation by Guanidine–Phenol Extraction
      296. Site-directed Mutagenesis of Double-stranded Plasmids
      297. Site-directed Mutagenesis Using PCR
      298. Site-specific Recombination: Uses in Biotechnology
      299. Small Silencing RNAs and Gene Therapy
      300. Small-Angle Scattering of Neutrons and X-Rays
      301. Smith–Waterman Algorithm
      302. snRNPs: Methods for Purification
      303. Somatic Cell Genetics
      304. Southern Blotting and Related DNA Detection Techniques
      305. Southern Blotting for the Analysis of Human Disease
      306. Staining, Viewing and Photography of Gels and Estimation of Fragment Sizes
      307. Statistical Methods in Physical Mapping
      308. Structural Genomics
      309. Structural Predictions and Modeling
      310. Studying Genomic and Epigenetic Aberrations by Microarray Profiling
      311. Surface Plasmon Resonance
      312. Systems Biology: Genomics Aspects
      313. Tooth Morphogenesis and Patterning: Molecular Genetics
      314. Transcription and Translation In Vitro
      315. Transcriptional Profiling in Plants
      316. Transcriptomics and Proteomics: Integration?
      317. Transfection of DNA into Mammalian Cells in Culture
      318. Transformation of Competent Bacterial Cells: Electroporation
      319. Transgenic Animals
      320. You have free access to this content
        Transgenic Mice
      321. Transgenic Mice Production
      322. Transgenic Plants
      323. Transgenic Xenopus Production
      324. Transgenic Zebrafish Production
      325. Translation of mRNAs in Xenopus Oocytes
      326. Translational Readthrough of Premature Termination Codons in a Therapeutic Context
      327. Transposon-Based Cellular Reprogramming to Induced Pluripotency
      328. Twin Study Contributions to Understanding Ontogeny
      329. Two-Hybrid and Related Systems
      330. Two-Hybrid Systems to Measure Protein–Protein Interactions
      331. Typing for Transplantation Antigens and Their Genes
      332. Vaccinia Virus Expression System
      333. Virus-Induced Human Oncogenesis
      334. Western Blotting: Immunoblotting
      335. Whole Animal Cloning
      336. Whole-genome Amplification
      337. X-Chromosome Dosage Compensation
      338. Xenopus as an Experimental Organism
      339. Yeast (YAC) and Human (HAC) Artificial Chromosome Clones
      340. Yeast Artificial Chromosomes
      341. Zebrafish as an Experimental Organism
    16. Transcription of DNA
    17. Transcriptional Regulation
    18. Translation of RNA
    19. Translational Regulation
  11. Neuroscience
  12. Plant Science
  13. Science & Society
  14. Structural Biology
  15. Virology

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