Current Protocols in Toxicology

Current Protocols in Toxicology

Online ISBN: 9780471140856

DOI: 10.1002/0471140856

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Current Protocols in Toxicology Supplements

2014:    Supplement 61     Supplement 60     Supplement 59    
2013:    Supplement 58     Supplement 57     Supplement 56     Supplement 55    
2012:    Supplement 54     Supplement 53     Supplement 52     Supplement 51    
2011:    Supplement 50     Supplement 49     Supplement 48     Supplement 47    
2010:    Supplement 46     Supplement 45     Supplement 44     Supplement 43    
2009:    Supplement 42     Supplement 41     Supplement 40     Supplement 39    
2008:    Supplement 38     Supplement 37     Supplement 36     Supplement 35    
2007:    Supplement 34     Supplement 33     Supplement 32     Supplement 31    
2006:    Supplement 30     Supplement 29     Supplement 28     Supplement 27    
2005:    Supplement 26     Supplement 25     Supplement 24     Supplement 23    
2004:    Supplement 22     Supplement 21     Supplement 20     Supplement 19    
2003:    Supplement 18     Supplement 17     Supplement 16     Supplement 15    
2002:    Supplement 14     Supplement 13     Supplement 12     Supplement 11    
2001:    Supplement 10     Supplement 9       Supplement 8       Supplement 7      
2000:    Supplement 6       Supplement 5       Supplement 4       Supplement 3      
1999:    Supplement 2       Supplement 1       Core publication




 Supplement 61, August 2014

Unit 1.13 Using the Cytosensor Microphysiometer to Assess Ocular Toxicity
         Abstract | Full Text:  HTML   PDF

Unit 23.7 Current Concepts in Drug-Induced Bile Salt Export Pump (BSEP) Interference
         Abstract | Full Text:  HTML   PDF

Unit 24.2 Isolation of Endothelial Cells from Mouse Lung
         Abstract | Full Text:  HTML   PDF

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 Supplement 60, May 2014

Unit 12.22 Synaptic Structure Quantification in Cultured Neurons
         Abstract | Full Text:  HTML   PDF

Unit 25.2 Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark-Type Oxygen Electrode
         Abstract | Full Text:  HTML   PDF

Unit 25.3 Assessment of Fatty Acid Beta Oxidation in Cells and Isolated Mitochondria
         Abstract | Full Text:  HTML   PDF

Unit 25.4 Identifying Compounds that Induce Opening of the Mitochondrial Permeability Transition Pore in Isolated Rat Liver Mitochondria
         Abstract | Full Text:  HTML   PDF

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 Supplement 59, February 2014

Unit 1.12 Developmental Toxicity Assays Using the Drosophila Model
         Abstract | Full Text:  HTML   PDF

Unit 12.21 Comparative Human and Rat “Neurosphere Assay” for Developmental Neurotoxicity Testing
         Abstract | Full Text:  HTML   PDF

Unit 25.1 High-Content Imaging Assays for Identifying Compounds that Generate Superoxide and Impair Mitochondrial Membrane Potential in Adherent Eukaryotic Cells
         Abstract | Full Text:  HTML   PDF

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 Supplement 58, November 2013

Unit 4.40 Multi-Parametric Analysis of Cell Death Pathways Using Live-Cell Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 17.16 Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
         Abstract | Full Text:  HTML   PDF

Unit 18.17 In Vitro Assays of Chemotaxis as a Window into Mechanisms of Toxicant-Induced Immunomodulation
         Abstract | Full Text:  HTML   PDF

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 Supplement 57, September 2013

Unit 6.17 Methods to Detect Protein Glutathionylation
         Abstract | Full Text:  HTML   PDF

Unit 21.12 In Vivo Measurement of Intragastric Pressure with a Rubber Balloon in the Anesthetized Rat
         Abstract | Full Text:  HTML   PDF

Unit 23.6 Assessment of Drug Transporter Function Using Fluorescent Cell Imaging
         Abstract | Full Text:  HTML   PDF

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 Supplement 56, May 2013

Unit 1.5 The Use of Fish-Derived Cell Lines for Investigation of Environmental Contaminants: An Update Following OECD’s Fish Toxicity Testing Framework N◦ 171
         Abstract | Full Text:  HTML   PDF

Unit 1.11 Minipigs: Applications in Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 4.39 Simultaneous, Untargeted Metabolic Profiling of Polar and Nonpolar Metabolites by LC-Q-TOF Mass
         Abstract | Full Text:  HTML   PDF

Unit 12.20 Assessment of Adult Neurogenesis in Mice
         Abstract | Full Text:  HTML   PDF

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 Supplement 55, February 2013

Unit 2.23 High-Throughput, Cell-Based Screens to Identify Small-Molecule Inhibitors of Ricin Toxin and Related Category B Ribosome Inactivating Proteins (RIPs)
         Abstract | Full Text:  HTML   PDF

Unit 14.11 High-Throughput Gene Silencing and mRNA Expression Analysis in Hepatocyte Sandwich Cultures
         Abstract | Full Text:  HTML   PDF

Unit 16.10 Preparation of Rodent Testis Co-Cultures
         Abstract | Full Text:  HTML   PDF

Unit 24.1 In Situ Measurement of Vapor Uptake in the Rodent Upper Respiratory Tract
         Abstract | Full Text:  HTML   PDF

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 Supplement 54, November 2012

Unit 20.8 The NCTC 2544 IL-18 Assay for the In Vitro Identification of Contact Allergens
         Abstract | Full Text:  HTML   PDF

Unit 21.11 Assessment of Intestinal Peristalsis in Vitro
         Abstract | Full Text:  HTML   PDF

Unit 23.4 The Vesicular Transport Assay: Validated In Vitro Methods to Study Drug-Mediated Inhibition of Canalicular Efflux Transporters ABCB11/BSEP and ABCC2/MRP2
         Abstract | Full Text:  HTML   PDF

Unit 23.5 Membrane Vesicle ABC Transporter Assays for Drug Safety Assessment
         Abstract | Full Text:  HTML   PDF

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 Supplement 53, August 2012

Unit 14.9 Assessing Cell Fusion and Cytokinesis Failure as Mechanisms of Clone 9 Hepatocyte Multinucleation In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 14.10 Assessment of Metabolic Stability Using the Rainbow Trout (Oncorhynchus mykiss) Liver S9 Fraction
         Abstract | Full Text:  HTML   PDF

Unit 18.16 Mining the Salivary Proteome with Grating-Coupled Surface Plasmon Resonance Imaging and Surface Plasmon Coupled Emission Microarrays
         Abstract | Full Text:  HTML   PDF

Unit 21.10 Evaluation of Gastric Ulcerogenic and Healing Impairment Effects of Bisphosphonates: Adverse Gastric Reactions of Bisphosphonate
         Abstract | Full Text:  HTML   PDF

Unit 23.3 Renal Transporters in Drug Disposition, Drug-Drug Interactions, and Nephrotoxicity
         Abstract | Full Text:  HTML   PDF

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 Supplement 52, May 2012

Unit 11.16 Morphological Assessment of Neurite Outgrowth in Hippocampal Neuron-Astrocyte Co-Cultures
         Abstract | Full Text:  HTML   PDF

Unit 23.1 Measuring Altered Disposition of Xenobiotics in Experimental Models of Liver Disease
         Abstract | Full Text:  HTML   PDF

Unit 23.2 Use of Zinc Finger Nuclease Technology to Knock Out Efflux Transporters in C2BBe1 Cells
         Abstract | Full Text:  HTML   PDF

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 Supplement 51, February 2012

Unit 4.38 CYP1B1 Detection
         Abstract | Full Text:  HTML   PDF

Unit 17.15 High-Throughput, Multiplexed Analysis of 3-Nitrotyrosine in Individual Proteins
         Abstract | Full Text:  HTML   PDF

Unit 18.15 Developmental Immunotoxicity (DIT): Assays for Evaluating Effects of Exogenous Agents on Development of the Immune System
         Abstract | Full Text:  HTML   PDF

Unit 20.7 The Local Lymph Node Assay (LLNA)
         Abstract | Full Text:  HTML   PDF

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 Supplement 50, November 2011

Unit 2.22 Murine Embryonic Stem Cell Derivation, In Vitro Pluripotency Characterization, and In Vivo Teratoma Formation
         Abstract | Full Text:  HTML   PDF

Unit 12.19 Methods to Assess Neuroinflammation
         Abstract | Full Text:  HTML   PDF

Unit 22.3 Preparation of Epithelial and Mesenchymal Stem Cells from Murine Mammary Gland
         Abstract | Full Text:  HTML   PDF

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 Supplement 49, August 2011

Unit 2.20 Assessment of Mitochondrial Toxicity in HepG2 Cells in Cultured in High-Glucose- or Galactose-Containing Media
         Abstract | Full Text:  HTML   PDF

Unit 2.21 Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.9 Overview of Peroxiredoxins in Oxidant Defense and Redox Regulation
         Abstract | Full Text:  HTML   PDF

Unit 7.10 Measurement of Peroxiredoxin Activity
         Abstract | Full Text:  HTML   PDF

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 Supplement 48, May 2011

Unit 2.19 Fluorescence Correlation Spectroscopy: Detecting and Interpreting the Mobility of Transmembrane Proteins In Vivo
         Abstract | Full Text:  HTML   PDF

Unit 3.11 High-Throughput Assays for Assessing Mitochondrial Dysfunction Caused by Compounds that Impair mtDNA-Encoded Protein Levels in Eukaryotic Cells
         Abstract | Full Text:  HTML   PDF

Unit 4.37 Aldehyde Reduction by Cytochrome P450
         Abstract | Full Text:  HTML   PDF

Unit 12.18 Cellular fura-2 Manganese Extraction Assay (CFMEA)
         Abstract | Full Text:  HTML   PDF

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 Supplement 47, February 2011

Unit 2.18 Role Integrative Signaling Through Gap Junctions in Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 20.6 Caco-2 Cells as a Model for Intestinal Absorption
         Abstract | Full Text:  HTML   PDF

Unit 22.1 Using Chemotherapeutic Drugs to Trigger Hematopoietic Stem Cell Activation and Determine Hematological Toxicity
         Abstract | Full Text:  HTML   PDF

Unit 22.2 Isolation and Applications of Prostate Side Population Cells Based on Dye Cycle Violet Efflux
         Abstract | Full Text:  HTML   PDF

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 Supplement 46, November 2010

Unit 12.16 Morphometric Analysis in Neurodegenerative Disorders
         Abstract | Full Text:  HTML   PDF

Unit 21.8 Assessment of Gastrointestinal Motility Using Three Different Assays In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 21.9 Assessment of Gastrointestinal Propulsive Activity Using Three Different Models of Peristalsis In Vivo in the Mouse
         Abstract | Full Text:  HTML   PDF

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 Supplement 45, August 2010

Unit 17.13 Transcription Factor Nrf2: Examination of Nuclear Protein Levels by Immunoblotting and Promoter Response Element Binding by Chromatin Immunoprecipitation (ChIP)
         Abstract | Full Text:  HTML   PDF

Unit 17.14 LC-MS/MS Quantitation of Mercapturic Acid Conjugates of Lipid Peroxidation Products as Markers of Oxidative Stress
         Abstract | Full Text:  HTML   PDF

Unit 21.5 Measurement of Gastric Acid Secretion in the Anaesthetized Rat
         Abstract | Full Text:  HTML   PDF

Unit 21.6 Endogenous Gastric Mediators: Patho-Physiological Role and Measurements
         Abstract | Full Text:  HTML   PDF

Unit 21.7 Measurement of Small Intestinal Damage
         Abstract | Full Text:  HTML   PDF

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 Supplement 44, May 2010

Unit 4.36 Measurement of Cysteine S-Conjugate β-Lyase Activity
         Abstract | Full Text:  HTML   PDF

Unit 14.8 Assessment of Mitochondrial Dysfunction Arising from Treatment with Hepatotoxicants
         Abstract | Full Text:  HTML   PDF

Unit 21.3 Assessment of Enteral Bacteria
         Abstract | Full Text:  HTML   PDF

Unit 21.4 Assessment of Ischemic Vascular Damage
         Abstract | Full Text:  HTML   PDF

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 Supplement 43, February 2010

Unit 4.35 Manipulation of Expression of Arsenic (+3 Oxidation State) Methyltransferase in Cultured Cells
         Abstract | Full Text:  HTML   PDF

Unit 12.16 Morphometric Analysis in Neurodegenerative Disorders
         Abstract | Full Text:  HTML   PDF

Unit 12.17 Neonatal Rat Primary Microglia: Isolation, Culturing, and Selected Applications
         Abstract | Full Text:  HTML   PDF

Unit 21.1 Overview of Gastrointestinal Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 21.2 Methods to Measure Gastric Mucosal Lesions in the Rat
         Abstract | Full Text:  HTML   PDF

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 Supplement 42, November 2009

Unit 4.30 Co-Oxidation by Cyclooxygenases
         Abstract | Full Text:  HTML   PDF

Unit 4.31 An Overview of Arsenic Metabolism and Toxicity
         Abstract | Full Text:  HTML   PDF

Unit 4.32 In Vitro Assays of Inorganic Arsenic Methylation
         Abstract | Full Text:  HTML   PDF

Unit 4.33 Analysis of Arsenical Metabolites in Biological Samples
         Abstract | Full Text:  HTML   PDF

Unit 4.34 Purification of Arsenic (+3 Oxidation State) Methyltransferase from Rat Liver Cytosol
         Abstract | Full Text:  HTML   PDF

Unit 12.15 Application of Single-Cell Microfluorimetry to Neurotoxicology Assays
         Abstract | Full Text:  HTML   PDF

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 Supplement 41, August 2009

Unit 2.17 Assessment of Gap Junctional Intercellular Communication
         Abstract | Full Text:  HTML   PDF

Unit 4.28 Azoreductase in Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Unit 4.29 Measurement of Phenylalanine Monooxygenase (PAH) Activities
         Abstract | Full Text:  HTML   PDF

Unit 5.8 Determination of ADME and Bioavailability Following Intravenous, Oral, and Dermal Routes of Exposure
         Abstract | Full Text:  HTML   PDF

Unit 17.12 Thioredoxin Redox Western Analysis
         Abstract | Full Text:  HTML   PDF

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 Supplement 40, May 2009

Unit 2.15 Current Concepts in Drug-Induced Mitochondrial Toxicity
         Abstract | Full Text:  HTML   PDF

Unit 2.16 Fluoroscent pH and Oxygen Probes of the Assessment of Mitochondrial Toxicity in Isolated Mitochondria and Whole Cells
         Abstract | Full Text:  HTML   PDF

Unit 17.11 Measuring Electrophile Stress
         Abstract | Full Text:  HTML   PDF

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 Supplement 39, February 2009

Unit 1.10 Use of Medaka in Toxicity Testing
         Abstract | Full Text:  HTML   PDF

Unit 4.27 Measurement of In Vitro Cytochrome P450 2B6 Activity
         Abstract | Full Text:  HTML   PDF

Unit 6.16 Glutamate Cysteine Ligase (GCL) Transgenic and Gene-Targeted Mice for Controlling Glutathione Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 12.14 Measurement of Isoprostanes as Markers of Oxidative Stress in Neuronal Tissue
         Abstract | Full Text:  HTML   PDF

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 Supplement 38, November 2008

Unit 4.26 Assays for S-Adenosylmethionine (AdoMet/SAM)-Dependent Methyltransferases
         Abstract | Full Text:  HTML   PDF

Unit 6.15 Measurement of Cysteine Dioxygenase Activity and Protein Abundance
         Abstract | Full Text:  HTML   PDF

Unit 17.2 Measurement of Protein Sulfenic Acid Content
         Abstract | Full Text:  HTML   PDF

Unit 17.10 Complex I Assay in Mitochondrial Preparations from CNS
         Abstract | Full Text:  HTML   PDF

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 Supplement 37, August 2008

Unit 14.7 A Rat Primary Hepatocyte Culture Model for Aging Studies
         Abstract | Full Text:  HTML   PDF

Unit 20.5 CFU-MK Assay for Acute Thrombocytopenia
         Abstract | Full Text:  HTML   PDF

Appendix 1A Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

Appendix 1C Radioactivity
         Abstract | Full Text:  HTML   PDF

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  Supplement 36, May 2008

Unit 2.14 Cross-Species Genetic Toxicity Assessment Accomplished by Flow Cytometric Analysis of Blood
         Abstract | Full Text:  HTML   PDF

Unit 18.14 Analysis of Modulation of Immunoglobulin Gene Expression
         Abstract | Full Text:  HTML   PDF

Unit 20.4 Neutral Red Uptake Cytotoxicity Tests for Estimating Starting Doses for Acute Oral Toxicity Tests
         Abstract | Full Text:  HTML   PDF

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  Supplement 35, February 2008

Unit 6.14 Measurement of Aminoacylases
         Abstract | Full Text:  HTML   PDF

Unit 11.15 Experimental Models of Hypoxic-Ischemic Encephalopathy: Hypoxia-Ischemia in the Immature Rat
         Abstract | Full Text:  HTML   PDF

Unit 17.9 Identification and Characterization of Oxylipid-Protein and Peptide Conjugates by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 20.3 The Syrian Hamster Embryo (SHE) Low pH Cell Transformation Assay
         Abstract | Full Text:  HTML   PDF

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  Supplement 34, November 2007

Unit 6.12 Glutathione-Dependent Bioactivation
         Abstract | Full Text:  HTML   PDF

Unit 6.13 Methods for Measuring Cysteine S -Conjugate β-Lyase Activity
         Abstract | Full Text:  HTML   PDF

Unit 12.13 Determination of Cholinesterase in Blood and Tissue
         Abstract | Full Text:  HTML   PDF

Unit 20.1 Overview of Alternative Methodologies in Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 20.2 CFU-GM Assay for Evaluation of Drug Myelotoxic Activity
         Abstract | Full Text:  HTML   PDF

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  Supplement 33, August 2007

Unit 4.23 Measurement of Soluble Epoxide Hydrolase (sEH) Activity
         Abstract | Full Text:  HTML   PDF

Unit 4.24 Enzymatic Activity of Human Carboxylesterases
         Abstract | Full Text:  HTML   PDF

Unit 4.25 In Vitro Evaluation of Metabolic Drug-Drug Interactions: A Descriptive and Critical Commentary
         Abstract | Full Text:  HTML   PDF

Unit 18.13 Fluorescence Microscopy and Flow Cytometric Analysis of Peyer's Patches and Intestinal Immune Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3B Techniques for Mammalian Cell Tissue Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 32, May 2007

Unit 4.22 Biochemical and Genetic Analysis of NAD(P)H:Quinone Oxidoreductase 1 (NQO1)
         Abstract | Full Text:  HTML   PDF

Unit 14.6 Measuring Covalent Binding in Hepatotoxicity
         Abstract | Full Text:  HTML   PDF

Appendix 3F One-Dimensional SDS Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 31, February 2007

Unit 1.9 The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and Compound Exposure Parameters
         Abstract | Full Text:  HTML   PDF

Unit 2.13 Mouse Models for Human Hemato-Lymphopoiesis
         Abstract | Full Text:  HTML   PDF

Unit 19.1 Toxicology Testing in Drug Discovery and Development
         Abstract | Full Text:  HTML   PDF

Unit 19.2 Toxicity Testing in Chemical Safety Evaluation
         Abstract | Full Text:  HTML   PDF

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  Supplement 30, November 2006

Unit 2.12 Detection of the Mitochondrial Apoptosis-Induced Channel (MAC) and Its Regulation by Bcl-2 Family Proteins
         Abstract | Full Text:  HTML   PDF

Unit 4.21 Monoamine Oxidase Assays
         Abstract | Full Text:  HTML   PDF

Unit 13.12 Analysis of Ultrasonic Vocalizations Emitted by Infant Rodents
         Abstract | Full Text:  HTML   PDF

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  Supplement 29, August 2006

Unit 4.20 NMR Analysis of Thiodiglycol Oxidation by Mammalian Alcohol Dehydrogenases
         Abstract | Full Text:  HTML   PDF

Unit 7.8 Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples
         Abstract | Full Text:  HTML   PDF

Unit 18.11 Analysis of Immunotoxicity by Enumeration of Antibody-Producing B Cells
         Abstract | Full Text:  HTML   PDF

Unit 18.12 The Reporter Antigen Popliteal Lymph Node Assay
         Abstract | Full Text:  HTML   PDF

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  Supplement 28, May 2006

Unit 3.10 Analysis of Mismatch Repair in Human Nuclear Extracts
         Abstract | Full Text:  HTML   PDF

Unit 6.10 Overview of Protein Glutathionylation
         Abstract | Full Text:  HTML   PDF

Unit 6.11 Measurement of Protein Glutathionylation
         Abstract | Full Text:  HTML   PDF

Unit 17.8 Detection of Stress Proteins as Biomarkers of Oxidative Stress
         Abstract | Full Text:  HTML   PDF

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  Supplement 27, February 2006

Unit 2.11 Single-Cell Analysis of Lipid Rafts in Lymphocytes and in T Cell–Containing Immunoconjugates
         Abstract | Full Text:  HTML   PDF

Unit 4.19 Measurement of Human Cytochrome P4501A2 ( CYP1A2 ) Activity In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 7.7 Catalase Activity Assays
         Abstract | Full Text:  HTML   PDF

Unit 13.11 Scoring Learning and Memory in Developing Rodents
         Abstract | Full Text:  HTML   PDF

Unit 18.10 Host Resistance Model to an Intracellular Pathogen
         Abstract | Full Text:  HTML   PDF

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  Supplement 26, November 2005

Unit 4.18 Aldehyde Dehydrogenases: Measurement of Activities and Protein Levels
         Abstract | Full Text:  HTML   PDF

Unit 13.9 Methods in the Analysis of Maternal Behavior in the Rodent
         Abstract | Full Text:  HTML   PDF

Unit 13.10 Scoring of Social Interactions and Play in Mice During Adolescence
         Abstract | Full Text:  HTML   PDF

Unit 16.9 Hershberger Assay to Investigate the Effects of Endocrine-Disrupting Compounds with Androgenic or Antiandrogenic Activity in Castrate-Immature Male Rats
         Abstract | Full Text:  HTML   PDF

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  Supplement 25, August 2005

Unit 4.17 Measurement of Xenobiotic Carbonyl Reduction in Human Liver Fractions
         Abstract | Full Text:  HTML   PDF

Unit 13.7 Overview of Behavioral Teratology
         Abstract | Full Text:  HTML   PDF

Unit 13.8 Statistical Analysis of Behavioral Data
         Abstract | Full Text:  HTML   PDF

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  Supplement 24, May 2005

Unit 4.16 Evaluation of the Cytochrome b 5 /Cytochrome b 5 Reductase Pathway
         Abstract | Full Text:  HTML   PDF

Unit 17.5 F 2 -Isoprostanes as Markers of Oxidant Stress: An Overview
         Abstract | Full Text:  HTML   PDF

Unit 17.6 Quantification of F 2 -Isoprostanes by Gas Chromatography/Mass Spectrometry as a Measure of Oxidant Stress
         Abstract | Full Text:  HTML   PDF

Unit 17.7 Immuno-Spin Trapping: Detection of Protein-Centered Radicals
         Abstract | Full Text:  HTML   PDF

Unit 18.9 In Vitro Model for Modulation of Helper T Cell Differentiation and Activation
         Abstract | Full Text:  HTML   PDF

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  Supplement 23, February 2005

Unit 11.14 Methods to Produce Brain Hyperthermia
         Abstract | Full Text:  HTML   PDF

Unit 18.7 Solid-Phase Immunoassays
         Abstract | Full Text:  HTML   PDF

Unit 18.8 Immune Cell Phenotyping Using Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Appendix 3I The Colorimetric Detection and Quantitation of Total Protein
         Abstract | Full Text:  HTML   PDF

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  Supplement 22, November 2004

Unit 4.15 TaqMan Real Time–Polymerase Chain Reaction Methods for Determination of Nucleotide Polymorphisms in Human N -Acetyltransferase-1 ( NAT1 ) and -2 ( NAT2 )
         Abstract | Full Text:  HTML   PDF

Unit 5.7 Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice
         Abstract | Full Text:  HTML   PDF

Unit 11.13 Detection and Assessment of Xenobiotic-Induced Sensory Neuropathy
         Abstract | Full Text:  HTML   PDF

Unit 12.12 Morphological Measurement of Neurotoxic Injury in the Peripheral Nervous System: Preparation of Material for Light and Transmission Electron Microscopic Evaluation
         Abstract | Full Text:  HTML   PDF

Unit 18.5 Measuring Lymphocyte Transcription Factor Activity by ELISA
         Abstract | Full Text:  HTML   PDF

Unit 18.6 Measuring the Activity of Cytolytic Lymphocytes
         Abstract | Full Text:  HTML   PDF

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  Supplement 21, August 2004

Unit 4.14 Biotransformation Studies Using Rat Proximal Tubule Cells
         Abstract | Full Text:  HTML   PDF

Unit 18.3 Murine Asthma Models
         Abstract | Full Text:  HTML   PDF

Unit 18.4 Use of Bronchoalveolar Lavage to Detect Lung Injury
         Abstract | Full Text:  HTML   PDF

Appendix 3G Spectrophotometric Determination of Protein Concentration
         Abstract | Full Text:  HTML   PDF

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  Supplement 20, May 2004

Unit 4.13 Human Cytochrome P450: Metabolism of Testosterone by CYP3A4 and Inhibition by Ketoconazole
         Abstract | Full Text:  HTML   PDF

Unit 17.4 In Situ Localization of Nonenzymatic Peroxidase-Like Activity of Tissue-Bound Transition Metals
         Abstract | Full Text:  HTML   PDF

Unit 18.1 Associating Changes in the Immune System with Clinical Diseases for Interpretation in Risk Assessment
         Abstract | Full Text:  HTML   PDF

Unit 18.2 Local Lymph Node Assays
         Abstract | Full Text:  HTML   PDF

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  Supplement 19, February 2004

Unit 2.10 Analysis of Mitochondrial Dysfunction During Cell Death
         Abstract | Full Text:  HTML   PDF

Unit 4.12 Determination of Paraoxonase 1 Status and Genotypes at Specific Polymorphic Sites
         Abstract | Full Text:  HTML   PDF

Unit 14.5 Measurement of Hepatobiliary Transport
         Abstract | Full Text:  HTML   PDF

Unit 16.8 Transgenerational (In Utero/Lactational) Exposure to Investigate the Effects of Endocrine Disrupting Compounds (EDCs) in Rats
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, November 2003

Unit 1.8 Preclinical Models of Parkinson's Disease
         Abstract | Full Text:  HTML   PDF

Unit 12.11 Determining the Ability of Xenobiotic Metals to Bind a Specific Protein Domain by Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 17.1 Formation and Functions of Protein Sulfenic Acids
         Abstract | Full Text:  HTML   PDF

Unit 17.2 Measurement of Protein Sulfenic Acid Content
         Full Text:  HTML   PDF

Unit 17.3 Fluorescence Microplate Reader Measurement of Tissue Susceptibility to Lipid Peroxidation
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, August 2003

Unit 1.7 Zebrafish: An Animal Model for Toxicological Studies
         Abstract | Full Text:  HTML   PDF

Unit 11.10 Principles of Electrophysiology: An Overview
         Abstract | Full Text:  HTML   PDF

Unit 11.11 Electrophysiological Studies of Neurotoxicants on Central Synaptic Transmission in Acutely Isolated Brain Slices
         Abstract | Full Text:  HTML   PDF

Unit 11.12 Whole-Cell Patch-Clamp Electrophysiology of Voltage-Sensitive Channels
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, May 2003

Unit 1.6 Sea Urchin Embryos and Larvae as Biosensors for Neurotoxicants
         Abstract | Full Text:  HTML   PDF

Unit 13.5 In Vivo Assessment of Prenatal Developmental Toxicity in Rodents
         Abstract | Full Text:  HTML   PDF

Unit 13.6 Organ Culture of Midfacial Tissue and Secondary Palate
         Abstract | Full Text:  HTML   PDF

Unit 16.7 Performing a Testicular Spermatid Head Count
         Abstract | Full Text:  HTML   PDF

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  Supplement 15, February 2003

Unit 1.5 The Use of Fish-Derived Cell Lines for Investigation of Environmental Contaminants
         Abstract | Full Text:  HTML   PDF

Unit 5.6 Toxicant Transport by P-Glycoprotein
         Abstract | Full Text:  HTML   PDF

Unit 6.9 Coenzyme A and Coenzyme A-Glutathione Mixed Disulfide Measurements by HPLC
         Abstract | Full Text:  HTML   PDF

Unit 9.9 Purification and Characterization of Heme Oxygenase
         Abstract | Full Text:  HTML   PDF

Unit 12.9 Aggregating Neural Cell Cultures
         Abstract | Full Text:  HTML   PDF

Unit 12.10 Coculturing Neurons and Glial Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 14, November 2002

Unit 4.11 Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl-CoA:Amino Acid N -Acyltransferase: The Amino Acid Conjugation Pathway
         Abstract | Full Text:  HTML   PDF

Unit 6.8 Measurement of Glutathione Conjugates
         Abstract | Full Text:  HTML   PDF

Unit 13.4 Using Chicken Embryos for Teratology Studies
         Abstract | Full Text:  HTML   PDF

Unit 14.4 Isolation of Liver Kupffer Cells
         Abstract | Full Text:  HTML   PDF

Unit 16.6 Epididymal Sperm Count
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, August 2002

Unit 4.9 Measurements of Flavin-Containing Monooxygenase (FMO) Activities
         Abstract | Full Text:  HTML   PDF

Unit 4.10 Assays for the Classification of Two Types of Esterases: Carboxylic Ester Hydrolases and Phosphoric Triester Hydrolases
         Abstract | Full Text:  HTML   PDF

Unit 16.4 Histopathology of the Male Reproductive System II: Interpretation
         Abstract | Full Text:  HTML   PDF

Unit 16.5 Monitoring Endocrine Function in Males: Using Intra-Atrial Cannulas to Monitor Plasma Hormonal Dynamics in Toxicology Experiments
         Abstract | Full Text:  HTML   PDF

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  Supplement 12, May 2002

Unit 3.8 Methods for Measuring DNA Adducts and Abasic Sites I: Isolation, Purification, and Analysis of DNA Adducts in Intact DNA
         Abstract | Full Text:  HTML   PDF

Unit 3.9 Methods for Measuring DNA Adducts and Abasic Sites II: Methods for Measurement of DNA Adducts
         Abstract | Full Text:  HTML   PDF

Unit 7.6 Measurement of Ascorbic Acid and Dehydroascorbic Acid in Biological Samples
         Abstract | Full Text:  HTML   PDF

Unit 9.8 Quantitation of Human Heme Oxygenase (HO-1) Copies by Competitive RT-PCR
         Abstract | Full Text:  HTML   PDF

Unit 16.3 Histopathology of the Male Reproductive System I: Techniques
         Abstract | Full Text:  HTML   PDF

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  Supplement 11, February 2002

Unit 4.8 Analysis of the Aryl Hydrocarbon Receptor (AhR) Signal Transduction Pathway
         Abstract | Full Text:  HTML   PDF

Unit 12.8 Measurement of Glial Fibrillary Acidic Protein
         Abstract | Full Text:  HTML   PDF

Unit 14.3 Small Animal Models of Hemorrhagic Shock–Induced Liver Dysfunction
         Abstract | Full Text:  HTML   PDF

Unit 16.1 In Vivo Models for Male Reproductive Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 16.2 Guidelines for Mating Rodents
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, November 2001

Unit 4.6 Measuring the Activity of Arylamine N -Acetyltransferase (NAT)
         Abstract | Full Text:  HTML   PDF

Unit 4.7 Measurement of Carboxylesterase (CES) Activities
         Abstract | Full Text:  HTML   PDF

Unit 15.3 Aggregation Chimeras (ES Cell–Embryo)
         Abstract | Full Text:  HTML   PDF

Unit 15.4 Reporter Genes to Detect Cre Excision in Mice
         Abstract | Full Text:  HTML   PDF

Appendix 3G Spectrophotometric and Colorimetric Determination of Protein Concentration
        Full Text:  HTML   PDF

Appendix 3H Dialysis and Concentration of Protein Solutions
         Abstract | Full Text:  HTML   PDF

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  Supplement 9, August 2001

Unit 1.4 DNA Microarrays: An Overview of Technologies and Applications to Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 5.5 Porcine Skin Flow-Through Diffusion Cell System
         Abstract | Full Text:  HTML   PDF

Unit 12.7 Isolation of Cerebellar Granule Cells from Neonatal Rats
         Abstract | Full Text:  HTML   PDF

Unit 15.1 Embryonic Stem (ES) Cell Culture Basics
         Abstract | Full Text:  HTML   PDF

Unit 15.2 Genotyping Embryonic Stem (ES) Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3F One-Dimensional SDS Gel Electrophoresis of Proteins
        Full Text:  HTML   PDF

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  Supplement 8, May 2001

Unit 4.5 Measurement of Aryl and Alcohol Sulfotransferase Activity
         Abstract | Full Text:  HTML   PDF

Unit 5.4 Isolated Perfused Porcine Skin Flap
         Abstract | Full Text:  HTML   PDF

Unit 6.7 Oxidant-Induced Regulation of Glutathione Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 7.5 Measurement of MnSOD and CuZnSOD Activity in Mammalian Tissue Homogenates
         Abstract | Full Text:  HTML   PDF

Unit 14.1 Overview of Hepatotoxicity
         Abstract | Full Text:  HTML   PDF

Unit 14.2 Preparation of Hepatocytes
         Abstract | Full Text:  HTML   PDF

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  Supplement 7, February 2001

Unit 2.9 Measurement of Expression of the HSP70 Protein Family
         Abstract | Full Text:  HTML   PDF

Unit 8.9 HPLC Methods for Analysis of Porphyrins in Biological Media
         Abstract | Full Text:  HTML   PDF

Unit 13.3 Micromass Cultures in Teratology
         Abstract | Full Text:  HTML   PDF

Appendix 3D Detection and Quantitation of Radiolabeled Proteins in Gels and Blots
         Abstract | Full Text:  HTML   PDF

Appendix 3E Northern Blot Analysis of RNA
         Abstract | Full Text:  HTML   PDF

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  Supplement 6, November 2000

Unit 9.7 Functional Analysis of the Heme Oxygenase-1 Gene Promoter
         Abstract | Full Text:  HTML   PDF

Unit 11.9 Mouse Models of Focal Cerebral Ischemia
         Abstract | Full Text:  HTML   PDF

Unit 12.6 Estimating Cell Number in the Central Nervous System by Stereological Methods: The Optical Disector and Fractionator
         Abstract | Full Text:  HTML   PDF

Unit 13.1 Overview of Teratology
         Abstract | Full Text:  HTML   PDF

Unit 13.2 Rat Embryo Cultures for In Vitro Teratology
         Abstract | Full Text:  HTML   PDF

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  Supplement 5, August 2000

Unit 2.7 In Situ Hybridization Histochemistry
         Abstract | Full Text:  HTML   PDF

Unit 2.8 Confocal Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 6.6 γ-Glutamyl Transpeptidase Activity Assay
         Abstract | Full Text:  HTML   PDF

Unit 7.4 Measurement of Thioredoxin and Thioredoxin Reductase
         Abstract | Full Text:  HTML   PDF

Unit 9.6 An HPLC Method to Detect Heme Oxygenase Activity
         Abstract | Full Text:  HTML   PDF

Unit 11.8 Mouse Models of Global Cerebral Ischemia
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, May 2000

Unit 5.3 Measurement of Disposition Half-Life, Clearance, and Residence Times
         Abstract | Full Text:  HTML   PDF

Unit 10.9 Inducible Nitric Oxide Synthase Expression
         Abstract | Full Text:  HTML   PDF

Unit 12.3 Culturing Rat Hippocampal Neurons
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures
         Abstract | Full Text:  HTML   PDF

Unit 12.5 Analytical Cytology: Applications to Neurotoxicology
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, February 2000

Unit 2.6 In Vitro Methods for Detecting Cytotoxicity
         Abstract | Full Text:  HTML   PDF

Unit 3.6 Detecting Epigenetic Changes: DNA Methylation
         Abstract | Full Text:  HTML   PDF

Unit 3.7 Assays for Detecting Chromosomal Aberrations
         Abstract | Full Text:  HTML   PDF

Unit 4.4 Detection of Metabolites Using High-Performance Liquid Chromatography and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 10.8 Methods for Distinguishing Nitrosative and Oxidative Chemistry of Reactive Nitrogen Oxide Species Derived from Nitric Oxide
         Abstract | Full Text:  HTML   PDF

Unit 12.1 Biochemical Approaches to Studying Neurotoxicity
         Abstract | Full Text:  HTML   PDF

Unit 12.2 Development of an In Vitro Blood-Brain Barrier
         Abstract | Full Text:  HTML   PDF

Appendix 3C Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, November 1999

Unit 3.5 Assays for DNA Damage
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Measurement of Ferrochelatase Activity
         Abstract | Full Text:  HTML   PDF

Unit 8.8 Measurement of Erythrocyte Protoporphyrin Concentration by Double Extraction and Spectrofluorometry
         Abstract | Full Text:  HTML   PDF

Unit 10.7 Immunocytochemical Analysis of Cyclic Nucleotides
         Abstract | Full Text:  HTML   PDF

Unit 11.6 Risk Assessment and Neurotoxicology
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Neurobehavioral Testing in Humans
         Abstract | Full Text:  HTML   PDF

Appendix 3B Techniques for Mammalian Cell Tissue Culture
        Full Text:  HTML   PDF

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  Supplement 1, August 1999

Unit 2.5 Measurements of Intracellular Free Calcium Concentration in Biological Systems
         Abstract | Full Text:  HTML   PDF

Unit 3.3 Mutagenesis Assays in Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.4 Cell Transformation Assays
         Abstract | Full Text:  HTML   PDF

Unit 8.6 Measurement of δ-Aminolevulinate Dehydratase Activity
         Abstract | Full Text:  HTML   PDF

Unit 9.5 Histochemical Analysis of Heme Degradation Enzymes
         Abstract | Full Text:  HTML   PDF

Unit 10.6 Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase Staining
         Abstract | Full Text:  HTML   PDF

Appendix 1B Transgenic and Gene-Targeted Mouse Lines for Toxicology Studies
         Full Text:  HTML   PDF

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  Core Publication, May 1999

Unit 1.1 Nonhuman Primates as Animal Models for Toxicology Research
         Abstract | Full Text:  HTML   PDF

Unit 1.2 Statistical Approaches to the Design of Toxicology Studies
         Abstract | Full Text:  HTML   PDF

Unit 1.3 Transgenic Animals in Toxicology
         Abstract | Full Text:  HTML   PDF

Unit 2.1 Current Concepts in Cell Toxicity
         Abstract | Full Text:  HTML   PDF

Unit 2.2 Determination of Apoptosis and Necrosis
         Abstract | Full Text:  HTML   PDF

Unit 2.3 Detection of Covalent Binding
         Abstract | Full Text:  HTML   PDF

Unit 2.4 Measurement of Lipid Peroxidation
         Abstract | Full Text:  HTML   PDF

Unit 3.1 The Salmonella (Ames) Test for Mutagenicity
         Abstract | Full Text:  HTML   PDF

Unit 3.2 Measurement of a Malondialdehyde-DNA Adduct
         Abstract | Full Text:  HTML   PDF

Unit 4.1 Measurement of Cytochrome P-450
         Abstract | Full Text:  HTML   PDF

Unit 4.2 Purification of Cytochrome P-450 Enzymes
         Abstract | Full Text:  HTML   PDF

Unit 4.3 Measurements of UDP- Glucuronosyltransferase (UGT) Activities
         Abstract | Full Text:  HTML   PDF

Unit 5.1 Measurement of Bioavailability: Measurement of Absorption Through Skin In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 5.2 Measurement of Bioavailability: Measuring Absorption Through Skin In Vivo in Rats and Humans
         Abstract | Full Text:  HTML   PDF

Unit 6.1 Overview of Glutathione Function and Metabolism
         Abstract | Full Text:  HTML   PDF

Unit 6.2 Measurement of Glutathione and Glutathione Disulfide
         Abstract | Full Text:  HTML   PDF

Unit 6.3 Measurement of Glutathione Transport
         Abstract | Full Text:  HTML   PDF

Unit 6.4 Measurement of Glutathione Transferases
         Abstract | Full Text:  HTML   PDF

Unit 6.5 HPLC-Based Assays for Enzymes of Glutathione Biosynthesis
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Analysis of Glutathione-Related Enzymes
         Abstract | Full Text:  HTML   PDF

Unit 7.2 Measurement of Glutathione Reductase Activity
         Abstract | Full Text:  HTML   PDF

Unit 7.3 Analysis of Superoxide Dismutase Activity
         Abstract | Full Text:  HTML   PDF

Unit 8.1 The Heme Biosynthesis Pathway and Clinical Manifestations of Abnormal Function
         Abstract | Full Text:  HTML   PDF

Unit 8.2 Measurement of ALA Synthase Activity
         Abstract | Full Text:  HTML   PDF

Unit 8.3 Measurement of Heme Concentration
         Abstract | Full Text:  HTML   PDF

Unit 8.4 Measurement of Uroporphyrinogen Decarboxylase Activity
         Abstract | Full Text:  HTML   PDF

Unit 8.5 Measurement of Protoporphyrinogen Oxidase Activity
         Abstract | Full Text:  HTML   PDF

Unit 9.1 Overview of Heme Degradation Pathway
         Abstract | Full Text:  HTML   PDF

Unit 9.2 Detection of Heme Oxygenase Activity by Measurement of CO
         Abstract | Full Text:  HTML   PDF

Unit 9.3 Detection of Heme Oxygenase 1 and 2 Proteins and Bilirubin Formation
         Abstract | Full Text:  HTML   PDF

Unit 9.4 Detection of Biliverdin Reductase Activity
         Abstract | Full Text:  HTML   PDF

Unit 10.1 Overview of the Pathway and Functions of Nitric Oxide
         Abstract | Full Text:  HTML   PDF

Unit 10.2 Assay of Tissue Activity of Nitric Oxide Synthase
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Detection of Nitrosated Proteins
         Abstract | Full Text:  HTML   PDF

Unit 10.4 Fluorometric Techniques for the Detection of Nitric Oxide and Metabolites
         Abstract | Full Text:  HTML   PDF

Unit 10.5 Measurement of cGMP and Soluble Guanylyl Cyclase Activity
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Overview of Neurotoxicology
         Abstract | Full Text:  HTML   PDF

Unit 11.2 Neurobehavioral Screening in Rodents
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Assessment of Spatial Memory
         Abstract | Full Text:  HTML   PDF

Unit 11.4 Advanced Behavioral Testing in Rodents: Assessment of Cognitive Function in Animals
         Abstract | Full Text:  HTML   PDF

Unit 11.5 Testing for Organophosphate-Induced Delayed Polyneuropathy
         Abstract | Full Text:  HTML   PDF

Appendix 1A Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

Appendix 2A Common Stock Solutions and Buffers
         Abstract | Full Text:  HTML   PDF

Appendix 2B Standard Laboratory Equipment
         Abstract | Full Text:  HTML   PDF

Appendix 3A Molecular Biology Techniques
         Abstract | Full Text:  HTML   PDF

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