Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

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Current Protocols in Protein Science Supplements

2014:    Supplement 76     Supplement 75     
2013:    Supplement 74     Supplement 73     Supplement 72     Supplement 71     
2012:    Supplement 70     Supplement 69     Supplement 68     Supplement 67
2011:    Supplement 66     Supplement 65     Supplement 64     Supplement 63
2010:    Supplement 62     Supplement 61     Supplement 60     Supplement 59
2009:    Supplement 58     Supplement 57     Supplement 56     Supplement 55
2008:    Supplement 54     Supplement 53     Supplement 52     Supplement 51
2007:    Supplement 50     Supplement 49     Supplement 48     Supplement 47    
2006:    Supplement 46     Supplement 45     Supplement 44     Supplement 43    
2005:    Supplement 42     Supplement 41     Supplement 40     Supplement 39    
2004:    Supplement 38     Supplement 37     Supplement 36     Supplement 35    
2003:    Supplement 34     Supplement 33     Supplement 32     Supplement 31    
2002:    Supplement 30     Supplement 29     Supplement 28     Supplement 27    
2001:    Supplement 26     Supplement 25     Supplement 24     Supplement 23    
2000:    Supplement 22     Supplement 21     Supplement 20     Supplement 19    
1999:    Supplement 18     Supplement 17     Supplement 16     Supplement 15    
1998:    Supplement 14     Supplement 13     Supplement 12     Supplement 11    
1997:    Supplement 10     Supplement 9       Supplement 8       Supplement 7      
1996:    Supplement 6       Supplement 5       Supplement 4       Supplement 3      
1995:    Supplement 2       Supplement 1       Core publication




  Supplement 76, April 2014

Unit 2.15 Bioinformatics Protocols in Glycomics and Glycoproteomics
         Abstract | Full Text:  HTML   PDF

Unit 23.4 Quantitative Protein Analysis Using Enzymatic [18O]Water Labeling
         Abstract | Full Text:  HTML   PDF

Unit 28.6 Overview of the Regulation of Disulfide Bond Formation in Peptide and Protein Folding
         Abstract | Full Text:  HTML   PDF

Unit 28.7 Chemical Methods for Producing Disulfide Bonds in Peptides and Proteins to Study Folding Regulation
         Abstract | Full Text:  HTML   PDF

Unit 28.8 Chemical Methods and Approaches to the Regioselective Formation of Multiple Disulfide Bonds
         Abstract | Full Text:  HTML   PDF

Unit 29.9 Expression and Purification of Haemophilus influenzae Rhomboid Intramembrane Protease GlpG for Structural Studies
         Abstract | Full Text:  HTML   PDF

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  Supplement 75, February 2014

Unit 12.12 Protein O-Mannosylation in Metazoan Organisms
         Abstract | Full Text:  HTML   PDF

Unit 19.24 Development and Use of IgM/J-Chain Fusion Proteins for Characterization of Immunoglobulin Superfamily Ligand-Receptor Interactions
         Abstract | Full Text:  HTML   PDF

Unit 22.2 Protein Profiling Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
         Abstract | Full Text:  HTML   PDF

Unit 27.7 Preparation and Use of Reverse Protein Microarrays
         Abstract | Full Text:  HTML   PDF

Unit 29.8 Membrane Transport Piece by Piece: Production of Transmembrane Peptides for Structural and Functional Studies
         Abstract | Full Text:  HTML   PDF

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  Supplement 74, November 2013

Unit 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface
         Abstract | Full Text:  HTML   PDF

Unit 17.17 Pulsed EPR Distance Measurements in Soluble Proteins by Site-Directed Spin Labeling (SDSL)
         Abstract | Full Text:  HTML   PDF

Unit 19.23 BioID: A Screen for Protein-Protein Interaction
         Abstract | Full Text:  HTML   PDF

Unit 29.6 High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 29.7 Methods for Studying Interactions of Detergents and Lipids with α-Helical and β-Barrel Integral Membrane Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 73, August 2013

Unit 3.9 Computational Large-Scale Mapping of Protein-Protein Interactions Using Structural Complexes
         Abstract | Full Text:  HTML   PDF

Unit 3.11 Quantitative Analysis of Surface Expression of Membrane Proteins Using Cold-Adapted Proteases
         Abstract | Full Text:  HTML   PDF

Unit 9.9 Overview of Affinity Tags for Protein Purification
         Abstract | Full Text:  HTML   PDF

Unit 14.15 Nonradioactive Analysis of Dynamic Protein Palmitoylation
         Abstract | Full Text:  HTML   PDF

Unit 27.6 Using Antibody Arrays to Measure Protein Abundance and Glycosylation: Considerations for Optimal Performance
         Abstract | Full Text:  HTML   PDF

Unit 30.1 Site-Specific Protein Labeling with SNAP-Tags
         Abstract | Full Text:  HTML   PDF

Unit 30.2 Protein knockouts in Living Eukaryotes Using deGradFP and Green Fluorescent Protein Fusion Targets
         Abstract | Full Text:  HTML   PDF

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  Supplement 72, April 2013

Unit 17.15 Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 17.16 Neutron Scattering Techniques and Applications in Structural Biology
         Abstract | Full Text:  HTML   PDF

Unit 27.1 Overview of Protein Microarrays
         Abstract | Full Text:  HTML   PDF

Unit 27.3 Protein Microarrays for Identification of Novel Extracellular Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 27.4 Preparation of Recombinant Protein Spotted Arrays for Proteome-Wide Identification of Kinase Targets
         Abstract | Full Text:  HTML   PDF

Unit 27.5 Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays
         Abstract | Full Text:  HTML   PDF

Unit 28.5 Refolding of SDS-Denatured Proteins Using Amphipathic Cosolvents and Osmolytes
         Abstract | Full Text:  HTML   PDF

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  Supplement 71, February 2013

Unit 5.25 Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 17.8 Raman Spectroscopy of Proteins and Nucleoproteins
         Abstract | Full Text:  HTML   PDF

Unit 20.12 Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
         Abstract | Full Text:  HTML   PDF

Unit 28.4 Determining the Conformational Stability of a Protein from Urea and Thermal Unfolding Curves
         Abstract | Full Text:  HTML   PDF

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  Supplement 70, November 2012

Unit 2.6 Protein Databases on the Internet
         Abstract | Full Text:  HTML   PDF

Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 14.14 Fluorescent Labeling of Specific Cysteine Residues Using CyMPL
         Abstract | Full Text:  HTML   PDF

Unit 17.14 Sample Preparation, Data Collection, and Preliminary Data Analysis in Biomolecular Solution X-Ray Scattering
         Abstract | Full Text:  HTML   PDF

Unit 21.22 Proteolytic Fingerprinting of Complex Biological Samples Using Combinatorial Libraries of Fluorogenic Probes
         Abstract | Full Text:  HTML   PDF

Unit 28.3 Circular Dichroism in Protein Folding Studies
         Abstract | Full Text:  HTML   PDF

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  Supplement 69, August 2012

Unit 6.12 Removal of Detergents from Proteins and Peptides in a Spin-Column Format
         Abstract | Full Text:  HTML   PDF

Unit 8.10 Isolation of Monoclonal Antibody Charge Variants by Displacement Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 14.13 Enrichment and Detection of Tyrosine-Nitrated Proteins
         Abstract | Full Text:  HTML   PDF

Unit 18.1 Introduction to Peptide Synthesis
         Abstract | Full Text:  HTML   PDF

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  Supplement 68, April 2012

Unit 10.1 One-Dimensional SDS Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 12.11 Use of CID/ETD Mass Spectrometry to Analyze Glycopeptides
         Abstract | Full Text:  HTML   PDF

Unit 28.2 Overview of Protein Folding Mechanisms: Experimental and Theoretical Approaches to Probing Energy Landscapes
         Abstract | Full Text:  HTML   PDF

Unit 29.5 HPLC-SEC Characterization of Membrane Protein-Detergent Complexes
         Abstract | Full Text:  HTML   PDF

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  Supplement 67, February 2012

Unit 19.22 Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
         Abstract | Full Text:  HTML   PDF

Unit 29.1 Introduction to Membrane Proteins
         Abstract | Full Text:  HTML   PDF

Unit 29.2 Overexpression of Membrane Proteins Using Pichia pastoris
         Abstract | Full Text:  HTML   PDF

Unit 29.3 Radioligand Binding Analysis as a Tool for Quality Control of GPCR Production for Structural Characterization: Adenosine A2aR as a Template for Study
         Abstract | Full Text:  HTML   PDF

Unit 29.4 Purification of the Human G Protein–Coupled Receptor Adenosine A2aR in a Stable and Functional Form Expressed in Pichia pastoris
         Abstract | Full Text:  HTML   PDF

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  Supplement 66, November 2011

Unit 2.14 Protein Structural Domains: Definition and Prediction
         Abstract | Full Text:  HTML   PDF

Unit 12.8 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
         Abstract | Full Text:  HTML   PDF

Unit 14.5 Analysis of Protein Ubiquitination
         Abstract | Full Text:  HTML   PDF

Unit 17.4 Crystallization of Macromolecules
         Abstract | Full Text:  HTML   PDF

Unit 26.7 Survey of Protein Engineering Strategies
         Abstract | Full Text:  HTML   PDF

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  Supplement 65, August 2011

Unit 13.12 In-Gel Phosphatase Assay Using Fluorogenic and Radioactive Substrates
         Abstract | Full Text:  HTML   PDF

Unit 17.13 Cryo-Electron Tomography for Structural Characterization of Macromolecular Complexes
         Abstract | Full Text:  HTML   PDF

Unit 18.13 Peptidomic Approaches to Study Proteolytic Activity
         Abstract | Full Text:  HTML   PDF

Unit 19.21 Identification of Protein-Protein Interactions by Surface Plasmon Resonance followed by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 23.5 Peptide Extraction from Formalin-Fixed Paraffin-Embedded Tissue
         Abstract | Full Text:  HTML   PDF

Unit 28.1 Fast Relaxation Imaging in Living Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 64, April 2011

Unit 12.10 Preparation and Analysis of Glycan Microarrays
         Abstract | Full Text:  HTML   PDF

Unit 17.5 Overview on the Use of NMR to Examine Protein Structure
         Abstract | Full Text:  HTML   PDF

Unit 18.12 Storage and Handling Guidelines for Custom Peptides
         Abstract | Full Text:  HTML   PDF

Unit 27.2 Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array-Based Functional Protein Studies
         Abstract | Full Text:  HTML   PDF

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  Supplement 63, February 2011

Unit 3.2 Quantitative Amino Acid Analysis
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Removal of N-Terminal Blocking Groups from Proteins
         Abstract | Full Text:  HTML   PDF

Unit 12.9 High-Throughput Lectin Microarray-Based Analysis of Live Cell Surface Glycosylation
         Abstract | Full Text:  HTML   PDF

Unit 14.6 Analysis of Protein S-Nitrosylation
         Abstract | Full Text:  HTML   PDF

Unit 16.13 Preparation and Analysis of Proteins and Peptides Using MALDI TOF/TOF Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 26.6 Site-Saturation Mutagenesis: A Powerful Tool for Structure- Based Design of Combinatorial Mutation Libraries
         Abstract | Full Text:  HTML   PDF

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  Supplement 62, November 2010

Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Glycans
         Abstract | Full Text:  HTML   PDF

Unit 13.11 Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
         Abstract | Full Text:  HTML   PDF

Unit 14.3 Analysis of Protein Prenylation In Vitro and In Vivo Using Functionalized Phosphoisoprenoids
         Abstract | Full Text:  HTML   PDF

Unit 16.1 Overview of Peptide and Protein Analysis by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 17.12 Native Mass Spectrometry as a Tool in Structural Biology
         Abstract | Full Text:  HTML   PDF

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  Supplement 61, August 2010

Unit 5.24 Strategies to Optimize Protein Expression in E. coli
         Abstract | Full Text:  HTML   PDF

Unit 6.11 Elastin-Like Polypeptides as a Purification Tag for Recombinant Proteins
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Reversed-Phase High Performance Liquid Chromatography of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 17.10 Application of Dynamic Light Scattering in Protein Crystallization
         Abstract | Full Text:  HTML   PDF

Unit 17.11 The STINT-NMR Method for Studying In-Cell Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 26.2 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras
         Abstract | Full Text:  HTML   PDF

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  Supplement 60, April 2010

Unit 3.6 Protein Biotinylation
         Abstract | Full Text:  HTML   PDF

Unit 7.13 Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins
         Abstract | Full Text:  HTML   PDF

Unit 25.3 The Publication and Database Deposition of Molecular Interaction Data
         Abstract | Full Text:  HTML   PDF

Unit 25.4 PRIDE: Data Submission and Analysis
         Abstract | Full Text:  HTML   PDF

Unit 25.5 Using Spectral Libraries for Peptide Identification from Tandem Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

APPENDIX 2B Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

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  Supplement 59, February 2010

Unit 4.9 Trehalose and Protein Stability
         Abstract | Full Text:  HTML   PDF

Unit 8.9 Displacement Chromatography of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 16.12 Rapid Detergent Removal from Peptide Samples with Ethyl Acetate for Mass Spectrometry Analysis
         Abstract | Full Text:  HTML   PDF

Unit 26.5 TimeSTAMP Tagging of Newly Synthesized Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 58, November 2009

Unit 11.9 Amino Acid Analysis
         Abstract | Full Text:  HTML   PDF

Unit 17.6 Investigating Solution-Phase Protein Structure and Dynamics by Hydrogen Exchange Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 17.7 Introduction to Atomic Force Microscopy (AFM) in Biology
         Abstract | Full Text:  HTML   PDF

Unit 26.4 Recombinant Protein Purification by Self-Cleaving Elastin-like Polypeptide Fusion Tag
         Abstract | Full Text:  HTML   PDF

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  Supplement 57, August 2009

Unit 11.10 N-Terminal Sequence Analysis of Proteins and Peptides
         Abstract | Full Text:  HTML   PDF

Unit 12.1 Overview of Glycoconjugate Analysis
         Abstract | Full Text:  HTML   PDF

Unit 12.2 Metabolic Radiolabeling of Animal Cell Glycoconjugates
         Abstract | Full Text:  HTML   PDF

Unit 18.2 Combinatorial Peptide Synthesis on a Microchip
         Abstract | Full Text:  HTML   PDF

Unit 19.2 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
         Abstract | Full Text:  HTML   PDF

Unit 19.20 Strep/FLAG Tandem Affinity Purification (SF-TAP) to Study Protein Interactions
         Abstract | Full Text:  HTML   PDF

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  Supplement 56, April 2009

Unit 2.1 Computational Methods for Protein Sequence Comparison and Search
         Abstract | Full Text:  HTML   PDF

Unit 5.23 Autoinduction of Protein Expression
         Abstract | Full Text:  HTML   PDF

Unit 15.3 Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation
         Abstract | Full Text:  HTML   PDF

Unit 14.4 Analysis of Oxidative Modification of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 19.5 Imaging Protein-Protein Interactions by Förster Resonance Energy Transfer (FRET) Microscopy in Live Cells
         Abstract | Full Text:  HTML   PDF

Unit 21.19 The CLIP-CHIP Oligonucleotide Microarray: Dedicated Array for Analysis of All Protease, Nonproteolytic Homolog, and Inhibitor Gene Transcripts in Human and Mouse
         Abstract | Full Text:  HTML   PDF

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  Supplement 55, February 2009

Unit 3.10 Microvolume Spectrophotometric and Fluorometric Determination of Protein Concentration
         Abstract | Full Text:  HTML   PDF

Unit 6.9 Overview on Concepts and Applications of Fab Antibody Fragments
         Abstract | Full Text:  HTML   PDF

Unit 6.10 E. coli Expression and Purification of Fab Antibody Fragments
         Abstract | Full Text:  HTML   PDF

Unit 14.2 Analysis of Protein Acylation
         Abstract | Full Text:  HTML   PDF

Unit 14.4 Analysis of Oxidative Modification of Proteins
         Full Text:  HTML   PDF

Unit 19.19 Tandem Affinity Purification of Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 54, November 2008

Unit 5.22 A Bacterial Cell-Free Expression System to Produce Membrane Proteins and Proteoliposomes: From cDNA to Functional Assay
         Abstract | Full Text:  HTML   PDF

Unit 14.11 Analysis of Protein Lysine Acetylation In Vitro and In Vivo
         Abstract | Full Text:  HTML   PDF

Unit 14.12 Determination of Protein Lysine Deacetylation
         Abstract | Full Text:  HTML   PDF

Unit 18.11 Expression and Purification of ZEBRA Fusion Proteins and Applications for the Delivery of Macromolecules into Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 19.18 Detection and Analysis of Protein-Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

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  Supplement 53, August 2008

Unit 4.8 The Use of Detergents to Purify Membrane Proteins
         Abstract | Full Text:  HTML   PDF

Unit 7.12 Determination of Membrane Protein Molecular Weight Using Sedimentation Equilibrium Analytical Ultracentrifugation
         Abstract | Full Text:  HTML   PDF

Unit 23.6 Sample Preparation and In-Solution Protease Digestion of Proteins for Chromatography-Based Proteomic Analysis
         Abstract | Full Text:  HTML   PDF

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  Supplement 52, May 2008

Unit 5.21 Recombinant Protein Complex Expression in E. coli
         Abstract | Full Text:  HTML   PDF

Unit 6.6 Expression and Purification of GST Fusion Proteins
         Abstract | Full Text:  HTML   PDF

Unit 9.3 Affinity Purification of Natural Ligands
         Abstract | Full Text:  HTML   PDF

Unit 19.17 Membrane-Based Yeast Two-Hybrid System to Detect Protein Interactions
         Abstract | Full Text:  HTML   PDF

Appendix 4K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 51, February 2008

Unit 5.20 MultiBac: Multigene Baculovirus-Based Eukaryotic Protein Complex Production
         Abstract | Full Text:  HTML   PDF

Unit 14.10 Analysis of Histone Modifications by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 14.11 Detection and Analysis of Protein-Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 18.9 Phage Display for Generating Peptide Reagents
         Abstract | Full Text:  HTML   PDF

Unit 18.10 Utilizing Peptide SPOT Arrays to Identify Protein Interactions
         Abstract | Full Text:  HTML   PDF

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  Supplement 50, November 2007

Unit 2.9 Comparative Protein Structure Modeling Using MODELLER
         Abstract | Full Text:  HTML   PDF

Unit 2.13 Protein Structure Analysis Online
         Abstract | Full Text:  HTML   PDF

Unit 5.19 Overview on the Expression of Toxic Gene Products in Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 18.8 Overview of Solid Phase Synthesis of "Difficult Peptide" Sequences
         Abstract | Full Text:  HTML   PDF

Unit 24.4 Phosphoproteomics
         Abstract | Full Text:  HTML   PDF

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  Supplement 49, August 2007

Unit 19.4 Detection of Protein-Protein Interactions by Coprecipitation
         Abstract | Full Text:  HTML   PDF

Unit 21.18 Proteomic Identification of Cellular Protease Substrates Using Isobaric Tags for Relative and Absolute Quantification (iTRAQ)
         Abstract | Full Text:  HTML   PDF

Unit 21.19 The CLIP-CHIP Oligonucleotide Microarray: Dedicated Array for Analysis of All Protease, Nonproteolytic Homolog, and Inhibitor Gene Transcripts in Human and Mouse
         Full Text:  HTML   PDF

Unit 21.20 Purification and Characterization of Gingipains
         Abstract | Full Text:  HTML   PDF

Unit 21.21 Microplate Assay For Cathepsin Detection in Viable Cells Using Derivatives of 4-Methoxy-ß-Naphthylamide
         Abstract | Full Text:  HTML   PDF

Unit 25.2 Overview of Tandem Mass Spectrometry (MS/MS) Database Search Algorithms
         Abstract | Full Text:  HTML   PDF

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  Supplement 48, May 2007

Unit 2.12 Minimotif Miner: A Computational Tool to Investigate Protein Function, Disease, and Genetic Diversity
         Abstract | Full Text:  HTML   PDF

Unit 3.4 Assays for Determination of Protein Concentration
         Abstract | Full Text:  HTML   PDF

Unit 10.6 Protein Detection in Gels Without Fixation
         Abstract | Full Text:  HTML   PDF

Unit 24.3 Glycoproteomics Using Chemical Immobilization
         Abstract | Full Text:  HTML   PDF

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  Supplement 47, February 2007

Unit 6.8 Automated Large-Scale Purification of a Recombinant G-Protein-Coupled Neurotensin Receptor
         Abstract | Full Text:  HTML   PDF

Unit 17.9 Crystallization of Integral Membrane Proteins
         Abstract | Full Text:  HTML   PDF

Unit 19.16 Determination of Protein Contacts by Chemical Cross-Linking With EDC and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 26.3 Incorporation of Isotopically Enriched Amino Acids
         Abstract | Full Text:  HTML   PDF

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  Supplement 46, November 2006

Unit 14.6 Analysis of Protein S-Nitrosylation
         Abstract | Full Text:  HTML   PDF

Unit 14.9 Identifying and Quantifying Sites of Protein Methylation by Heavy Methyl SILAC
         Abstract | Full Text:  HTML   PDF

Unit 19.15 Identifying Small-Molecule Modulators of Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 20.10 Circular Dichroism to Study Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 20.11 Quantitative Determination of Protein Stability and Ligand Binding by Pulse Proteolysis
         Abstract | Full Text:  HTML   PDF

Appendix 3C Techniques for Mammalian Cell Tissue Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 45, August 2006

Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
         Abstract | Full Text:  HTML   PDF

Unit 19.12 Fluorescence Quenching Methods to Study Lipid-Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 19.13 Overview of Biacore Systems and Their Applications
         Abstract | Full Text:  HTML   PDF

Unit 19.14 Using Biacore to Measure the Binding Kinetics of an Antibody-Antigen Interaction
         Abstract | Full Text:  HTML   PDF

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  Supplement 44, May 2006

Unit 5.18 Wheat Germ Cell-Free Expression System for Protein Production
         Abstract | Full Text:  HTML   PDF

Unit 14.8 Analysis of Protein Sumoylation
         Abstract | Full Text:  HTML   PDF

Unit 24.2 Organelle Proteomics
         Abstract | Full Text:  HTML   PDF

Unit 26.2 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras
         Abstract | Full Text:  HTML   PDF

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  Supplement 43, February 2006

Unit 12.6 Determining the Structure of Oligosaccharides N- and O-Linked to Glycoproteins
         Abstract | Full Text:  HTML   PDF

Unit 12.7 Determining the Structure of Glycan Moieties by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 19.11 Production and Use of Trimeric Isoleucine Zipper Fusion Proteins to Study Surface Receptor Ligand Interactions
         Abstract | Full Text:  HTML   PDF

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  Supplement 42, November 2005

Unit 17.2 Electron Microscopy and Image Processing: An Essential Tool for Structural Analysis of Macromolecules
         Abstract | Full Text:  HTML   PDF

Unit 24.1 Human Serum and Plasma Proteomics
         Abstract | Full Text:  HTML   PDF

Unit 25.1 Bioinformatics Analysis for Interactive Proteomics
         Abstract | Full Text:  HTML   PDF

Unit 26.1 Misincorporation Proton-Alkyl Exchange (MPAX): Engineering Cysteine Probes into Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 41, August 2005

Unit 2.10 Protein Charge Determination
         Abstract | Full Text:  HTML   PDF

Unit 2.11 Basic Protein Sequence Analysis
         Abstract | Full Text:  HTML   PDF

Unit 6.2 Preparation of Soluble Proteins from Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 10.12 Overview of Digital Electrophoresis Analysis
         Abstract | Full Text:  HTML   PDF

Unit 19.10 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
         Abstract | Full Text:  HTML   PDF

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  Supplement 40, May 2005

Unit 8.8 Expanded-Bed Adsorption Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 18.7 Guide for Resin and Linker Selection in Solid-Phase Peptide Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 20.9 Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

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  Supplement 39, February 2005

Unit 14.7 Tyrosine O-Sulfation
         Abstract | Full Text:  HTML   PDF

Unit 20.8 Spectroscopic Methods for the Determination of Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 22.6 Protein Profiling by Microscale Solution Isoelectrofocusing (MicroSol-IEF)
         Abstract | Full Text:  HTML   PDF

Unit 23.5 Protein Microarrays
         Abstract | Full Text:  HTML   PDF

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  Supplement 38, November 2004

Unit 4.4 Desalting, Concentration, and Buffer Exchange by Dialysis and Ultrafiltration
         Abstract | Full Text:  HTML   PDF

Unit 4.7 Extraction of Proteins from Plant Tissues
         Abstract | Full Text:  HTML   PDF

Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 7.6 Determining the CD Spectrum of a Protein
         Abstract | Full Text:  HTML   PDF

Unit 7.7 Determining the Fluorescence Spectrum of a Protein
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Enzymatic Digestion of Proteins in Gels for Mass Spectrometric Identification and Structural Analysis
         Abstract | Full Text:  HTML   PDF

Unit 19.9 Identifying Protein Interactions by Hydroxyl-Radical Protein Footprinting
         Abstract | Full Text:  HTML   PDF

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  Supplement 37, August 2004

Unit 4.1 Overview of Cell Fractionation
         Abstract | Full Text:  HTML   PDF

Unit 4.2 Purification of Organelles from Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 11.11 Determination of Disulfide-Bond Linkages in Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 36, May 2004

Unit 9.9 Overview of Affinity Tags for Protein Purification
         Abstract | Full Text:  HTML   PDF

Unit 14.6 Analysis of Protein S-Nitrosylation
        Full Text:  HTML   PDF

Unit 21.17 Applications for Chemical Probes of Proteolytic Activity
         Abstract | Full Text:  HTML   PDF

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  Supplement 35, February 2004

Unit 17.1 Overview of Protein Structural and Functional Folds
         Abstract | Full Text:  HTML   PDF

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  Supplement 34, November 2003

Unit 13.6 Preparation and Application of Polyclonal and Monoclonal Sequence-Specific Anti-Phosphoamino Acid Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 17.4 Crystallization of Macromolecules
         Abstract | Full Text:  HTML   PDF

Unit 23.1 Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 23.2 Quantitative Protein Profile Comparisons Using the Isotope-Coded Affinity Tag Method
         Abstract | Full Text:  HTML   PDF

Unit 23.3 Proteomic Analysis Using 2-D Liquid Separations of Intact Proteins From Whole-Cell Lysates
         Abstract | Full Text:  HTML   PDF

Unit 23.4 Quantitative Protein Analysis Using Proteolytic [ 18 O]Water Labeling
         Abstract | Full Text:  HTML   PDF

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  Supplement 33, August 2003

Unit 2.6 Protein Databases on the Internet
         Abstract | Full Text:  HTML   PDF

Unit 3.1 Spectrophotometric Determination of Protein Concentration
         Abstract | Full Text:  HTML   PDF

Unit 17.8 Raman Spectroscopy of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 21.15 Zymography of Metalloproteinases
         Abstract | Full Text:  HTML   PDF

Unit 21.16 Monitoring Metalloproteinase Activity Using Synthetic Fluorogenic Substrates
         Abstract | Full Text:  HTML   PDF

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  Supplement 32, May 2003

Unit 4.3 Subcellular Fractionation of Tissue Culture Cells
         Abstract | Full Text:  HTML   PDF

Unit 21.14 Expression, Purification, and Characterization of Aspartic Endopeptidases: Plasmodium Plasmepsins and "Short" Recombinant Human Pseudocathepsin
         Abstract | Full Text:  HTML   PDF

Unit 22.4 Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison
         Abstract | Full Text:  HTML   PDF

Unit 22.5 Isolation of Organelles and Prefractionation of Protein Extracts Using Free-Flow Electrophoresis
         Abstract | Full Text:  HTML   PDF

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  Supplement 31, February 2003

Unit 7.11 Rapid Screening of E. coli Extracts by Heteronuclear NMR
         Abstract | Full Text:  HTML   PDF

Unit 11.8 C-Terminal Sequence Analysis
         Abstract | Full Text:  HTML   PDF

Unit 13.10 Use of Protein Phosphatase Inhibitors
         Abstract | Full Text:  HTML   PDF

Unit 20.7 Analytical Ultracentrifugation: Sedimentation Velocity Analysis
         Abstract | Full Text:  HTML   PDF

Unit 22.3 Laser Capture Microdissection for Proteome Analysis
         Abstract | Full Text:  HTML   PDF

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  Supplement 30, December 2002

Unit 1.5 Overview of the Physical State of Proteins within Cells
         Abstract | Full Text:  HTML   PDF

Unit 5.17 Use of the Gateway System for Protein Expression in Multiple Hosts
         Abstract | Full Text:  HTML   PDF

Unit 6.1 Overview of the Purification of Recombinant Proteins Produced in Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 21.13 Expression, Purification, and Characterization of Caspases
         Abstract | Full Text:  HTML   PDF

Unit 22.1 Overview of Proteome Analysis
         Abstract | Full Text:  HTML   PDF

Unit 22.2 Protein Profiling Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
         Abstract | Full Text:  HTML   PDF

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  Supplement 29, September 2002

Unit 1.4 Purification of Glutamate Dehydrogenase from Liver and Brain
         Abstract | Full Text:  HTML   PDF

Unit 10.5 Protein Detection in Gels Using Fixation
         Abstract | Full Text:  HTML   PDF

Unit 14.5 Analysis of Protein Ubiquitination
         Abstract | Full Text:  HTML   PDF

Unit 17.7 Introduction to Atomic Force Microscopy (AFM) in Biology
         Full Text:  HTML   PDF

Appendix 4J The Polymerase Chain Reaction
         Abstract | Full Text:  HTML   PDF

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  Supplement 28, June 2002

Unit 2.9 Comparative Protein Structure Prediction
        Full Text:  HTML   PDF

Unit 17.6 Probing Protein Structure and Dynamics by Hydrogen Exchange–Mass Spectrometry
         Full Text:  HTML   PDF

Unit 18.3 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
         Abstract | Full Text:  HTML   PDF

Appendix 2A Laboratory Safety
         Abstract | Full Text:  HTML   PDF

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  Supplement 27, March 2002

Unit 4.6 Long-Term Storage of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 16.11 De Novo Peptide Sequencing via Manual Interpretation of MS/MS Spectra
         Abstract | Full Text:  HTML   PDF

Unit 19.8 Scintillation Proximity Assay ( SPA ) Technology to Study Biomolecular Interactions
         Abstract | Full Text:  HTML   PDF

Unit 21.11 Over-Expression and Purification of Active Serine Proteases and Their Variants from Escherichia coli Inclusion Bodies
         Abstract | Full Text:  HTML   PDF

Unit 21.12 Assaying Proteases in Cellular Environments
         Abstract | Full Text:  HTML   PDF

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  Supplement 26, December 2001

Unit 3.1 Spectrophotometric Determination of Protein Concentration

Unit 3.9 Solid-Phase Profiling of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 18.1 Introduction to Peptide Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 21.7 Serpins (Serine Protease Inhibitors)
         Abstract | Full Text:  HTML   PDF

Unit 21.8 Caspases
         Abstract | Full Text:  HTML   PDF

Unit 21.9 Use of GFP as a Reporter for the Analysis of Sequence-Specific Proteases
         Abstract | Full Text:  HTML   PDF

Unit 21.10 An Overview of Serine Proteases
         Abstract | Full Text:  HTML   PDF

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  Supplement 25, September 2001

Unit 12.8 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
         Abstract | Full Text:  HTML   PDF

Unit 19.7 Identification of Protein Interactions by Far Western Analysis
         Abstract | Full Text:  HTML   PDF

Unit 20.6 Size-Exclusion Chromatography with On-Line Light Scattering
         Abstract | Full Text:  HTML   PDF

Unit 21.6 Purification of the Eukaryotic 20S Proteasome
         Abstract | Full Text:  HTML   PDF

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  Supplement 24, June 2001

Unit 11.6 Reversed-Phase Isolation of Peptides
         Abstract | Full Text:  HTML   PDF

Unit 19.6 High-Throughput Screening for Protein-Protein Interactions Using Yeast Two-Hybrid Arrays
         Abstract | Full Text:  HTML   PDF

Unit 21.4 Metalloproteases
         Abstract | Full Text:  HTML   PDF

Unit 21.5 Purification and Characterization of Proteasomes from Saccharomyces cerevisiae
         Abstract | Full Text:  HTML   PDF

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  Supplement 23, March 2001

Unit 8.7 HPLC of Peptides and Proteins
         Abstract | Full Text:  HTML   PDF

Unit 18.6 Disulfide Bond Formation in Peptides
         Abstract | Full Text:  HTML   PDF

Unit 19.5 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
         Full Text:  HTML   PDF

Unit 21.3 Overview of Pepsin-like Aspartic Peptidases
         Abstract | Full Text:  HTML   PDF

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  Supplement 22, December 2000

Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
        Full Text:  HTML   PDF

Unit 12.6 Determining the Structure of Oligosaccharides N- and O-Linked to Glycoproteins
        Full Text:  HTML   PDF

Unit 12.7 Determining the Structure of Glycan Moieties by Mass Spectrometry
        Full Text:  HTML   PDF

Unit 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface
         Abstract | Full Text:  HTML   PDF

Unit 16.9 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using Microscale Capillary Liquid Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 16.10 Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching
         Abstract | Full Text:  HTML   PDF

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  Supplement 21, September 2000

Unit 21.1 Proteases
         Abstract | Full Text:  HTML   PDF

Unit 21.2 Papain-like Cysteine Proteases
         Abstract | Full Text:  HTML   PDF

Appendix 3G Statistics: Detecting Differences Among Groups
         Abstract | Full Text:  HTML   PDF

Appendix 3H Analyzing Radioligand Binding Data
         Abstract | Full Text:  HTML   PDF

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  Supplement 20, June 2000

Unit 2.4 Internet Basics
         Abstract | Full Text:  HTML   PDF

Unit 3.8 Analysis of Selenocysteine-Containing Proteins
         Abstract | Full Text:  HTML   PDF

Unit 5.16 Choice of Cellular Protein Expression System
         Abstract | Full Text:  HTML   PDF

Unit 14.4 Analysis of Oxidative Modification of Proteins
         Full Text:  HTML   PDF

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  Supplement 19, March 2000

Unit 2.6 Protein Databases on the Internet

Unit 2.7 Protein Tertiary Structure Prediction
         Abstract | Full Text:  HTML   PDF

Unit 2.8 Protein Tertiary Structure Modeling
         Abstract | Full Text:  HTML   PDF

Unit 11.4 Chemical Cleavage of Proteins in Solution
         Abstract | Full Text:  HTML   PDF

Unit 11.5 Chemical Cleavage of Proteins on Membranes
         Abstract | Full Text:  HTML   PDF

Unit 17.5 Introduction to NMR of Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, December 1999

Unit 9.8 Immunoprecipitation
         Abstract | Full Text:  HTML   PDF

Unit 13.9 Phosphopeptide Mapping and Identification of Phosphorylation Sites
         Abstract | Full Text:  HTML   PDF

Unit 20.3 Analytical Centrifugation: Equilibrium Approach
         Abstract | Full Text:  HTML   PDF

Unit 20.4 Titration Microcalorimetry
         Abstract | Full Text:  HTML   PDF

Unit 20.5 Reduced-Scale Large-Zone Analytical Gel-Filtration Chromatography for Measurement of Protein Association Equilibria
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, September 1999

Unit 3.7 Metabolic Labeling with Amino Acids
         Abstract | Full Text:  HTML   PDF

Unit 18.5 Synthesis and Application of Peptide Dendrimers As Protein Mimetics
         Abstract | Full Text:  HTML   PDF

Unit 19.4 Detection of Protein-Protein Interactions by Coprecipitation
        Full Text:  HTML   PDF

Unit 20.1 Overview of the Quantitation of Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 20.2 Measuring Protein Interactions by Optical Biosensors
         Abstract | Full Text:  HTML   PDF

Appendix 1D Common Conversion Factors
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, June 1999

Unit 7.10 Characterizing Recombinant Proteins Using HPLC Gel Filtration and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 10.13 Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers
         Abstract | Full Text:  HTML   PDF

Appendix 5A Theoretical Aspects of the Quantitative Characterization of Ligand Binding
         Abstract | Full Text:  HTML   PDF

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  Supplement 15, March 1999

Unit 2.5 Sequence Similarity Searching Using the BLAST Family of Programs
         Abstract | Full Text:  HTML   PDF

Unit 8.2 Ion-Exchange Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 16.7 Enzymatic Approaches for Obtaining Amino Acid Sequence: On-Target Ladder Sequencing
         Abstract | Full Text:  HTML   PDF

Unit 18.4 Native Chemical Ligation of Polypeptides
         Abstract | Full Text:  HTML   PDF

Unit 19.3 Phage-Based Expression Cloning to Identify Interacting Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 14, December 1998

Unit 16.3 Sample Preparation for MALDI Mass Analysis of Peptides and Proteins
         Abstract | Full Text:  HTML   PDF

Unit 16.4 In-Gel Digestion of Proteins for MALDI-MS Fingerprint Mapping
         Abstract | Full Text:  HTML   PDF

Unit 16.5 Searching Sequence Databases Over the Internet: Protein Identification Using MS-Fit
         Abstract | Full Text:  HTML   PDF

Unit 16.6 Searching Sequence Databases Over the Internet: Protein Identification Using MS-Tag
         Abstract | Full Text:  HTML   PDF

Unit 19.1 Analysis of Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

Unit 19.2 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
         Full Text:  HTML   PDF

Appendix 4L Growth and Manipulation of Yeast
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, September 1998

Unit 5.11 Overview of the Vaccinia Virus Expression System
         Abstract | Full Text:  HTML   PDF

Unit 5.12 Preparation of Cell Cultures and Vaccinia Virus Stocks
         Abstract | Full Text:  HTML   PDF

Unit 5.13 Generation of Recombinant Vaccinia Viruses
         Abstract | Full Text:  HTML   PDF

Unit 5.14 Characterization of Recombinant Vaccinia Viruses and Their Products
         Abstract | Full Text:  HTML   PDF

Unit 5.15 Gene Expression Using the Vaccinia Virus/ T7 RNA Polymerase Hybrid System
         Abstract | Full Text:  HTML   PDF

Appendix 3E Silanizing Glassware
         Abstract | Full Text:  HTML   PDF

Appendix 3F Protein Precipitation Using Ammonium Sulfate
         Abstract | Full Text:  HTML   PDF

Appendix 4 Molecular Biology Techniques
         Abstract | Full Text:  HTML
Appendix 4A Media Preparation and Bacteriological Tools
         Abstract | Full Text:  HTML   PDF

Appendix 4B Growth in Liquid or Solid Media
         Abstract | Full Text:  HTML   PDF

Appendix 4C Preparation of Plasmid DNA
         Abstract | Full Text:  HTML   PDF

Appendix 4D Introduction of Plasmid DNA into Cells
         Abstract | Full Text:  HTML   PDF

Appendix 4E Purification and Concentration of DNA from Aqueous Solutions
         Abstract | Full Text:  HTML   PDF

Appendix 4F Agarose Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Appendix 4G Southern Blotting
         Abstract | Full Text:  HTML   PDF

Appendix 4H Hybridization Analysis of DNA Blots
         Abstract | Full Text:  HTML   PDF

Appendix 4I Digestion of DNA with Restriction Endonucleases
         Abstract | Full Text:  HTML   PDF

Appendix 4K Quantitation of Nucleic Acids with Absorption Spectroscopy
         Full Text:  HTML   PDF

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  Supplement 12, June 1998

Unit 5.10 Production of Recombinant Proteins in Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.9 Measuring Protein Thermostability by Differential Scanning Calorimetry
         Abstract | Full Text:  HTML   PDF

Unit 9.7 Purification of DNA -Binding Proteins Using Biotin/Streptavidin Affinity Systems
         Abstract | Full Text:  HTML   PDF

Unit 17.4 Crystallization of Soluble Macromolecules

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  Supplement 11, February 1998

Unit 2.4 Internet Basics

Unit 7.8 Light Scattering
         Abstract | Full Text:  HTML   PDF

Unit 9.6 Purification of Sequence-Specific DNA -Binding Proteins by Affinity Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 10.4 Two-Dimensional Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 10.12 Digital Electrophoresis Analysis
        Full Text:  HTML   PDF

Appendix 1B Commonly Used Detergents
         Abstract | Full Text:  HTML   PDF

Appendix 1C Conversion Factors and Half-Life Information for Radioactivity
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, December 1997

Unit 5.9 Overview of Protein Expression by Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 6.7 Expression and Purification of Thioredoxin Fusion Proteins
         Abstract | Full Text:  HTML   PDF

Unit 10.11 Autoradiography
         Abstract | Full Text:  HTML   PDF

Unit 13.2 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation
         Abstract | Full Text:  HTML   PDF

Unit 13.8 Permeabilization Strategies to Study Protein Phosphorylation
         Abstract | Full Text:  HTML   PDF

Unit 17.3 Principles of Macromolecular X-Ray Crystallography
         Abstract | Full Text:  HTML   PDF

Unit 18.3 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins

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  Supplement 9, September 1997

Unit 6.6 Expression and Purification of GST Fusion Proteins
         Full Text:  HTML   PDF

Unit 13.7 Assays of Protein Kinases Using Exogenous Substrates
         Abstract | Full Text:  HTML   PDF

Unit 17.2 Overview of Macromolecular Electron Microscopy: An Essential Tool in Protein Structural Analysis
        Full Text:  HTML   PDF

Unit 18.1 Introduction to Peptide Synthesis

Unit 18.2 Synthesis of Multiple Peptides on Plastic Pins
         Full Text:  HTML   PDF

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  Supplement 8, June 1997

Unit 8.6 Hydroxylapatite Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 11.10 N-Terminal Sequence Analysis of Proteins and Peptides
         Full Text:  HTML   PDF

Unit 17.1 Overview of Protein Structural and Functional Folds

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  Supplement 7, March 1997

Unit 4.5 Selective Precipitation of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 11.6 Reversed-Phase Isolation of Peptides

Unit 11.9 Amino Acid Analysis
         Full Text:  HTML   PDF

Appendix 3C Techniques for Mammalian Cell Tissue Culture
        Full Text:  HTML   PDF

Appendix 3D Importing Biological Materials
         Abstract | Full Text:  HTML   PDF

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  Supplement 6, December 1996

Unit 2.4 Internet Basics

Unit 2.5 Sequence Similarity Searching Using the BLAST Family of Programs

Unit 3.6 Biotinylation of Proteins in Solution and on Cell Surfaces
         Full Text:  HTML   PDF

Unit 7.7 Determining the Fluorescence Spectrum of a Protein
        Full Text:  HTML   PDF

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  Supplement 5, September 1996

Unit 3.5 Kinetic Assay Methods
         Abstract | Full Text:  HTML   PDF

Unit 7.6 Determining the CD Spectrum of a Protein
        Full Text:  HTML   PDF

Unit 13.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides

Unit 14.2 Analysis of Protein Acylation
         Full Text:  HTML   PDF

Unit 14.3 Analysis of Protein Prenylation and Carboxyl-Methylation
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, June 1996

Unit 7.5 Analytical Ultracentrifugation
         Abstract | Full Text:  HTML   PDF

Unit 10.10 Immunoblot Detection
         Abstract | Full Text:  HTML   PDF

Unit 11.8 C-Terminal Sequence Analysis

Unit 15.2 Modification of Amino Groups
         Abstract | Full Text:  HTML   PDF

Unit 16.1 Overview of Peptide and Protein Analysis by Mass Spectrometry
         Abstract | Full Text:  HTML   PDF

Unit 16.2 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, March 1996

Unit 7.3 Determining the Identity and Structure of Recombinant Proteins
         Abstract | Full Text:  HTML   PDF

Unit 7.4 Transverse Urea-Gradient Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 9.5 Immunoaffinity Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Removal of N-Terminal Blocking Groups from Proteins
         Abstract | Full Text:  HTML   PDF

Unit 14.1 Analysis of Disulfide Bond Formation
         Abstract | Full Text:  HTML   PDF

Unit 15.1 Modification of Cysteine
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, December 1995

Unit 4.4 Concentration, Desalting, and Buffer Exchange

Unit 5.6 Overview of Protein Expression in Saccharomyces cerevisiae
         Abstract | Full Text:  HTML   PDF

Unit 5.7 Overview of Protein Expression in Pichia pastoris
         Abstract | Full Text:  HTML   PDF

Unit 5.8 Culture of Yeast for the Production of Heterologous Proteins
         Abstract | Full Text:  HTML   PDF

Unit 8.5 Chromatofocusing
         Abstract | Full Text:  HTML   PDF

Unit 10.9 Capillary Electrophoresis of Proteins and Peptides
         Abstract | Full Text:  HTML   PDF

Unit 12.5 Detection of Glycophospholipid Anchors on Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 1, September 1995

Unit 3.4 Assays for Total Protein
        Full Text:  HTML   PDF

Unit 4.3 Subcellular Fractionation

Unit 7.2 Determining the Identity and Purity of Recombinant Proteins by UV Absorption Spectroscopy
         Abstract | Full Text:  HTML   PDF

Unit 9.4 Metal-Chelate Affinity Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides

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  Core Publication, June 1995

Unit 1.1 Overview of Protein Purification and Characterization
         Abstract | Full Text:  HTML   PDF

Unit 1.2 Strategies for Protein Purification
         Abstract | Full Text:  HTML   PDF

Unit 1.3 Protein Purification Flow Charts
         Abstract | Full Text:  HTML   PDF

Unit 2.1 Computational Methods for Protein Sequence Analysis
         Full Text:  HTML   PDF

Unit 2.2 Hydrophobicity Profiles for Protein Sequence Analysis
         Abstract | Full Text:  HTML   PDF

Unit 2.3 Protein Secondary Structure Prediction
         Abstract | Full Text:  HTML   PDF

Unit 3.1 Spectrophotometric Determination of Protein Concentration

Unit 3.2 Quantitative Amino Acid Analysis
         Abstract | Full Text:  HTML   PDF

Unit 3.3 In Vitro Radiolabeling of Peptides and Proteins
         Abstract | Full Text:  HTML   PDF

Unit 4.1 Overview of Cell Fractionation

Unit 4.2 Purification of Organelles from Mammalian Cells

Unit 5.1 Production of Recombinant Proteins in Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 5.2 Selection of Escherichia coli Expression Systems
         Abstract | Full Text:  HTML   PDF

Unit 5.3 Fermentation and Growth of Escherichia coli for Optimal Protein Production
         Abstract | Full Text:  HTML   PDF

Unit 5.4 Overview of the Baculovirus Expression System
         Abstract | Full Text:  HTML   PDF

Unit 5.5 Protein Expression in the Baculovirus System
         Abstract | Full Text:  HTML   PDF

Unit 6.1 Overview of the Purification of Recombinant Proteins Produced in Escherichia coli

Unit 6.2 Preparation of Soluble Proteins from Escherichia coli

Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli

Unit 6.4 Overview of Protein Folding
         Abstract | Full Text:  HTML   PDF

Unit 6.5 Folding and Purification of Insoluble (Inclusion Body) Proteins from Escherichia coli
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Overview of the Characterization of Recombinant Proteins
         Abstract | Full Text:  HTML   PDF

Unit 8.1 Overview of Conventional Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 8.2 Ion-Exchange Chromatography

Unit 8.3 Gel-Filtration Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 8.4 Hydrophobic-Interaction Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 9.1 Lectin Affinity Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 9.2 Dye Affinity Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 9.3 Affinity Purification of Natural Ligands
         Full Text:  HTML   PDF

Unit 10.1 One-Dimensional SDS Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 10.2 One-Dimensional Isoelectric Focusing of Proteins in Slab Gels
         Abstract | Full Text:  HTML   PDF

Unit 10.3 One-Dimensional Electrophoresis Using Nondenaturing Conditions
         Abstract | Full Text:  HTML   PDF

Unit 10.4 Two-Dimensional Gel Electrophoresis

Unit 10.5 Protein Detection in Gels Using Fixation

Unit 10.6 Protein Detection in Gels Without Fixation

Unit 10.7 Electroblotting from Polyacrylamide Gels
         Abstract | Full Text:  HTML   PDF

Unit 10.8 Detection of Proteins on Blot Membranes
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Enzymatic Digestion of Proteins in Solution
         Abstract | Full Text:  HTML   PDF

Unit 11.2 Enzymatic Digestion of Proteins on PVDF Membranes
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Enzymatic Digestion of Proteins in Gels for Mass Spectrometric Identification and Structural Analysis

Unit 11.4 Chemical Cleavage of Proteins in Solution

Unit 11.5 Chemical Cleavage of Proteins on Membranes

Unit 11.6 Reversed-Phase Isolation of Peptides

Unit 12.1 Overview of Glycoconjugate Analysis
         Full Text:  HTML   PDF

Unit 12.2 Metabolic Radiolabeling of Animal Cell Glycoconjugates
         Full Text:  HTML   PDF

Unit 12.3 Inhibition of N-Linked Glycosylation
         Abstract | Full Text:  HTML   PDF

Unit 13.1 Overview of Protein Phosphorylation
         Abstract | Full Text:  HTML   PDF

Unit 13.2 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation

Unit 13.3 Phosphoamino Acid Analysis
         Abstract | Full Text:  HTML   PDF

Unit 13.4 Detection of Phosphorylation by Immunological Techniques
         Abstract | Full Text:  HTML   PDF

Unit 13.5 Detection of Phosphorylation by Enzymatic Techniques
         Abstract | Full Text:  HTML   PDF

Appendix 1A Characteristics of Amino Acids
         Abstract | Full Text:  HTML   PDF

Appendix 2A Laboratory Safety

Appendix 2B Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

Appendix 2C Centrifuges and Rotors
         Abstract | Full Text:  HTML   PDF

Appendix 2D Standard Laboratory Equipment
         Abstract | Full Text:  HTML   PDF

Appendix 2E Commonly Used Reagents
         Abstract | Full Text:  HTML   PDF

Appendix 3A Use of Protein Folding Reagents
         Abstract | Full Text:  HTML   PDF

Appendix 3B Dialysis
         Abstract | Full Text:  HTML   PDF

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