Current Protocols in Protein Science
Online ISBN: 9780471140863
DOI: 10.1002/0471140864
Archive
Current Protocols in Protein Science Supplements
2017: Supplement 88 Supplement 87
2016: Supplement 86 Supplement 85 Supplement 84 Supplement 83
2015: Supplement 82 Supplement 81 Supplement 80 Supplement 79
2014: Supplement 78 Supplement 77 Supplement 76 Supplement 75
2013: Supplement 74 Supplement 73 Supplement 72 Supplement 71
2012: Supplement 70 Supplement 69 Supplement 68 Supplement 67
2011: Supplement 66 Supplement 65 Supplement 64 Supplement 63
2010: Supplement 62 Supplement 61 Supplement 60 Supplement 59
2009: Supplement 58 Supplement 57 Supplement 56 Supplement 55
2008: Supplement 54 Supplement 53 Supplement 52 Supplement 51
2007: Supplement 50 Supplement 49 Supplement 48 Supplement 47
2006: Supplement 46 Supplement 45 Supplement 44 Supplement 43
2005: Supplement 42 Supplement 41 Supplement 40 Supplement 39
2004: Supplement 38 Supplement 37 Supplement 36 Supplement 35
2003: Supplement 34 Supplement 33 Supplement 32 Supplement 31
2002: Supplement 30 Supplement 29 Supplement 28 Supplement 27
2001: Supplement 26 Supplement 25 Supplement 24 Supplement 23
2000: Supplement 22 Supplement 21 Supplement 20 Supplement 19
1999: Supplement 18 Supplement 17 Supplement 16 Supplement 15
1998: Supplement 14 Supplement 13 Supplement 12 Supplement 11
1997: Supplement 10 Supplement 9 Supplement 8 Supplement 7
1996: Supplement 6 Supplement 5 Supplement 4 Supplement 3
1995: Supplement 2 Supplement 1 Core publication
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Supplement 88, April 2017
Unit 2.16 Computational Prediction of Intrinsic Disorder in Proteins
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Unit 6.14 N-Terminal Methionine Processing
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Unit 10.10 Immunoblotting and Immunodetection
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Unit 19.27 Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Bound Protein Clusters
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Unit 19.30 Bioluminescence Resonance Energy Transfer (BRET)-Based Synthetic Sensor Platform for Drug Discovery
Abstract | Full Text: HTML PDF
Unit 20.2 Measuring Protein Interactions by Optical Biosensors
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Supplement 87, February 2017
Unit 14.11 Assays for Acetylation and Other Acylations of Lysine Residues
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Unit 14.16 Detection and Measurement of Methionine Oxidation in Proteins
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Unit 15.1 Modification of Cysteine
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Unit 19.29 Non-Gradient Blue Native Polyacrylamide Gel Electrophoresis
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Unit 24.9 Characterization of Protein Content Present in Exosomes Isolated from Conditioned Media and Urine
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Unit 24.10 Analysis of Protein O-GlcNAcylation by Mass Spectrometry
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Supplement 86, November 2016
Unit 2.3 Computational Prediction of Protein Secondary Structure from Sequence
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Unit 2.9 Comparative Protein Structure Modeling Using MODELLER
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Unit 10.8 Detection of Proteins on Blot Membranes
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Unit 15.2 Modification of Amino Groups
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Unit 16.4 Mass Spectrometry-Based Bottom-Up Proteomics: Sample Preparation, LC-MS/MS Analysis, and Database Query Strategies
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Unit 24.8 Global Proteomics Analysis of Protein Lysine Methylation
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Supplement 85, August 2016
Unit 5.26 Use of the SHuffle Strains in Production of Proteins
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Unit 11.6 Separation of Peptides on HALO 2-Micron Particles
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Unit 17.7 Introduction to Atomic Force Microscopy (AFM) in Biology
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Unit 19.28 Designing and Applying Proximity-Dependent Hybridization Chain Reaction
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Unit 29.17 Correlative Förster Resonance Electron Transfer-Proximity Ligation Assay (FRET-PLA) Technique for Studying Interactions Involving Membrane Proteins
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Unit 29.18 Rapid Preparation of a Plasma Membrane Fraction: Western Blot Detection of Translocated Glucose Transporter 4 from Plasma Membrane of Muscle and Adipose Cells and Tissues
Abstract | Full Text: HTML PDF
Supplement 84, April 2016
Unit 4.8 The Use of Detergents to Purify Membrane Proteins
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Unit 6.13 Obtaining Soluble Folded Proteins from Inclusion Bodies Using Sarkosyl, Triton X-100, and CHAPS: Application to LB and M9 Minimal Media
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Unit 29.16 In Situ Proteolysis for Crystallization of Membrane Bound Cytochrome P450 17A1 and 17A2 Proteins from Zebrafish
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Appendix 3A Use of Protein Folding Reagents
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Appendix 3F Protein Precipitation Using Ammonium Sulfate
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Supplement 83, February 2016
Unit 4.5 Selective Precipitation of Proteins
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Unit 14.8 Analysis of Protein Sumoylation
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Unit 21.16 Handling Metalloproteinases
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Unit 28.10 Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System
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Unit 29.15 Expression, Solubilization, and Purification of Bacterial Membrane Proteins
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Supplement 82, November 2015
Unit 10.7 Electroblotting from Polyacrylamide Gels
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Unit 17.2 Electron Microscopy and Image Processing: Essential Tools for Structural Analysis of Macromolecules
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Unit 23.2 The Isotope-Coded Affinity Tag Method for Quantitative Protein Profile Comparison and Relative Quantitation of Cysteine Redox Modifications
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Unit 24.7 Large-Scale Identification of the Arginine Methylome by Mass Spectrometry
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Supplement 81, August 2015
Unit 17.18 Overview of Probing Protein-Ligand Interactions Using NMR
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Unit 24.6 Using Single Lectins to Enrich Glycoproteins in Conditioned Media
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Unit 29.12 Detergent Analysis in Protein Samples Using Mid-Infrared (MIR) Spectroscopy
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Unit 29.13 Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance
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Unit 29.14 Cell-Free Expression of G Protein–Coupled Receptors
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Supplement 80, April 2015
Unit 6.1 Overview of the Purification of Recombinant Proteins
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Unit 9.10 Protein Purification Using PDZ Affinity Chromatography
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Unit 14.7 Detection of Tyrosine Sulfation on Proteins
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Unit 19.27 Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Bound Protein Clusters
Abstract | Full Text: HTML PDF
Unit 22.4 Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison
Abstract | Full Text: HTML PDF
Supplement 79, February 2015
Unit 15.4 Application of meta- and para-Phenylenediamine as Enhanced Oxime Ligation Catalysts for Protein Labeling, PEGylation, Immobilization, and Release
Abstract | Full Text: HTML PDF
Unit 19.25 Measuring Protein-Protein and Protein-Nucleic Acid Interactions by Biolayer Interferometry
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Unit 19.26 Analysis of Protein Ligand-Receptor Binding by Photoaffinity Cross-Linking
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Unit 28.9 Analysis of Protein Stability and Ligand Interactions by Thermal Shift Assay
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Unit 30.4 Ligand-Directed Profiling of Organelles with Internalizing Phage Libraries
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Supplement 78, November 2014
Unit 6.2 Preparation of Soluble Proteins from Escherichia coli
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Unit 6.5 Folding and Purification of Insoluble (Inclusion Body) Proteins from Escherichia coli
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Unit 23.1 Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry
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Unit 24.5 Proteomic Analysis of Protein Deamidation
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Unit 30.3 Pulse-Chase Analysis for Studying Protein Synthesis and Maturation
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Supplement 77, August 2014
Unit 16.2 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides
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Unit 20.13 Thermodynamic Analysis of Weak Protein Interactions Using Sedimentation Equilibrium
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Unit 23.7 Middle-Down and Top-Down Mass Spectrometric Analysis of Co-occurring Histone Modifications
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Unit 27.8 Post-Translational Modification profiling—a High-Content Assay for Identifying Protein Modifications in Mammalian Cellular Systems
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Unit 29.10 Tetra Detector Analysis of Membrane Proteins
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Unit 29.11 General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays
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Supplement 76, April 2014
Unit 2.15 Bioinformatics Protocols in Glycomics and Glycoproteomics
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Unit 23.4 Quantitative Protein Analysis Using Enzymatic [18O]Water Labeling
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Unit 28.6 Overview of the Regulation of Disulfide Bond Formation in Peptide and Protein Folding
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Unit 28.7 Chemical Methods for Producing Disulfide Bonds in Peptides and Proteins to Study Folding Regulation
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Unit 28.8 Chemical Methods and Approaches to the Regioselective Formation of Multiple Disulfide Bonds
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Unit 29.9 Expression and Purification of Haemophilus influenzae Rhomboid Intramembrane Protease GlpG for Structural Studies
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Supplement 75, February 2014
Unit 12.12 Protein O-Mannosylation in Metazoan Organisms
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Unit 19.24 Development and Use of IgM/J-Chain Fusion Proteins for Characterization of Immunoglobulin Superfamily Ligand-Receptor Interactions
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Unit 22.2 Protein Profiling Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
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Unit 27.7 Preparation and Use of Reverse Protein Microarrays
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Unit 29.8 Membrane Transport Piece by Piece: Production of Transmembrane Peptides for Structural and Functional Studies
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Supplement 74, November 2013
Unit 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface
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Unit 17.17 Pulsed EPR Distance Measurements in Soluble Proteins by Site-Directed Spin Labeling (SDSL)
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Unit 19.23 BioID: A Screen for Protein-Protein Interaction
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Unit 29.6 High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
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Unit 29.7 Methods for Studying Interactions of Detergents and Lipids with α-Helical and β-Barrel Integral Membrane Proteins
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Supplement 73, August 2013
Unit 3.9 Computational Large-Scale Mapping of Protein-Protein Interactions Using Structural Complexes
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Unit 3.11 Quantitative Analysis of Surface Expression of Membrane Proteins Using Cold-Adapted Proteases
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Unit 9.9 Overview of Affinity Tags for Protein Purification
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Unit 14.15 Nonradioactive Analysis of Dynamic Protein Palmitoylation
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Unit 27.6 Using Antibody Arrays to Measure Protein Abundance and Glycosylation: Considerations for Optimal Performance
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Unit 30.1 Site-Specific Protein Labeling with SNAP-Tags
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Unit 30.2 Protein knockouts in Living Eukaryotes Using deGradFP and Green Fluorescent Protein Fusion Targets
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Supplement 72, April 2013
Unit 17.15 Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy
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Unit 17.16 Neutron Scattering Techniques and Applications in Structural Biology
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Unit 27.1 Overview of Protein Microarrays
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Unit 27.3 Protein Microarrays for Identification of Novel Extracellular Protein-Protein Interactions
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Unit 27.4 Preparation of Recombinant Protein Spotted Arrays for Proteome-Wide Identification of Kinase Targets
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Unit 27.5 Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays
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Unit 28.5 Refolding of SDS-Denatured Proteins Using Amphipathic Cosolvents and Osmolytes
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Supplement 71, February 2013
Unit 5.25 Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins
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Unit 17.8 Raman Spectroscopy of Proteins and Nucleoproteins
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Unit 20.12 Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
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Unit 28.4 Determining the Conformational Stability of a Protein from Urea and Thermal Unfolding Curves
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Supplement 70, November 2012
Unit 2.6 Protein Databases on the Internet
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Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
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Unit 14.14 Fluorescent Labeling of Specific Cysteine Residues Using CyMPL
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Unit 17.14 Sample Preparation, Data Collection, and Preliminary Data Analysis in Biomolecular Solution X-Ray Scattering
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Unit 21.22 Proteolytic Fingerprinting of Complex Biological Samples Using Combinatorial Libraries of Fluorogenic Probes
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Unit 28.3 Circular Dichroism in Protein Folding Studies
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Supplement 69, August 2012
Unit 6.12 Removal of Detergents from Proteins and Peptides in a Spin-Column Format
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Unit 8.10 Isolation of Monoclonal Antibody Charge Variants by Displacement Chromatography
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Unit 14.13 Enrichment and Detection of Tyrosine-Nitrated Proteins
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Unit 18.1 Introduction to Peptide Synthesis
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Supplement 68, April 2012
Unit 10.1 One-Dimensional SDS Gel Electrophoresis of Proteins
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Unit 12.11 Use of CID/ETD Mass Spectrometry to Analyze Glycopeptides
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Unit 28.2 Overview of Protein Folding Mechanisms: Experimental and Theoretical Approaches to Probing Energy Landscapes
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Unit 29.5 HPLC-SEC Characterization of Membrane Protein-Detergent Complexes
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Supplement 67, February 2012
Unit 19.22 Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
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Unit 29.1 Introduction to Membrane Proteins
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Unit 29.2 Overexpression of Membrane Proteins Using Pichia pastoris
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Unit 29.3 Radioligand Binding Analysis as a Tool for Quality Control of GPCR Production for Structural Characterization: Adenosine A2aR as a Template for Study
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Unit 29.4 Purification of the Human G Protein–Coupled Receptor Adenosine A2aR in a Stable and Functional Form Expressed in Pichia pastoris
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Supplement 66, November 2011
Unit 2.14 Protein Structural Domains: Definition and Prediction
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Unit 12.8 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
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Unit 14.5 Analysis of Protein Ubiquitination
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Unit 17.4 Crystallization of Macromolecules
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Unit 26.7 Survey of Protein Engineering Strategies
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Supplement 65, August 2011
Unit 13.12 In-Gel Phosphatase Assay Using Fluorogenic and Radioactive Substrates
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Unit 17.13 Cryo-Electron Tomography for Structural Characterization of Macromolecular Complexes
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Unit 18.13 Peptidomic Approaches to Study Proteolytic Activity
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Unit 19.21 Identification of Protein-Protein Interactions by Surface Plasmon Resonance followed by Mass Spectrometry
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Unit 23.5 Peptide Extraction from Formalin-Fixed Paraffin-Embedded Tissue
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Unit 28.1 Fast Relaxation Imaging in Living Cells
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Supplement 64, April 2011
Unit 12.10 Preparation and Analysis of Glycan Microarrays
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Unit 17.5 Overview on the Use of NMR to Examine Protein Structure
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Unit 18.12 Storage and Handling Guidelines for Custom Peptides
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Unit 27.2 Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array-Based Functional Protein Studies
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Supplement 63, February 2011
Unit 3.2 Quantitative Amino Acid Analysis
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Unit 11.7 Removal of N-Terminal Blocking Groups from Proteins
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Unit 12.9 High-Throughput Lectin Microarray-Based Analysis of Live Cell Surface Glycosylation
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Unit 14.6 Analysis of Protein S-Nitrosylation
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Unit 16.13 Preparation and Analysis of Proteins and Peptides Using MALDI TOF/TOF Mass Spectrometry
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Unit 26.6 Site-Saturation Mutagenesis: A Powerful Tool for Structure- Based Design of Combinatorial Mutation Libraries
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Supplement 62, November 2010
Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Glycans
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Unit 13.11 Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
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Unit 14.3 Analysis of Protein Prenylation In Vitro and In Vivo Using Functionalized Phosphoisoprenoids
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Unit 16.1 Overview of Peptide and Protein Analysis by Mass Spectrometry
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Unit 17.12 Native Mass Spectrometry as a Tool in Structural Biology
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Supplement 61, August 2010
Unit 5.24 Strategies to Optimize Protein Expression in E. coli
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Unit 6.11 Elastin-Like Polypeptides as a Purification Tag for Recombinant Proteins
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Unit 8.7 Reversed-Phase High Performance Liquid Chromatography of Proteins
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Unit 17.10 Application of Dynamic Light Scattering in Protein Crystallization
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Unit 17.11 The STINT-NMR Method for Studying In-Cell Protein-Protein Interactions
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Unit 26.2 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras
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Supplement 60, April 2010
Unit 3.6 Protein Biotinylation
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Unit 7.13 Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins
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Unit 25.3 The Publication and Database Deposition of Molecular Interaction Data
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Unit 25.4 PRIDE: Data Submission and Analysis
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Unit 25.5 Using Spectral Libraries for Peptide Identification from Tandem Mass Spectrometry
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APPENDIX 2B Safe Use of Radioisotopes
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Supplement 59, February 2010
Unit 4.9 Trehalose and Protein Stability
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Unit 8.9 Displacement Chromatography of Proteins
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Unit 16.12 Rapid Detergent Removal from Peptide Samples with Ethyl Acetate for Mass Spectrometry Analysis
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Unit 26.5 TimeSTAMP Tagging of Newly Synthesized Proteins
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Supplement 58, November 2009
Unit 11.9 Amino Acid Analysis
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Unit 17.6 Investigating Solution-Phase Protein Structure and Dynamics by Hydrogen Exchange Mass Spectrometry
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Unit 17.7 Introduction to Atomic Force Microscopy (AFM) in Biology
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Unit 26.4 Recombinant Protein Purification by Self-Cleaving Elastin-like Polypeptide Fusion Tag
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Supplement 57, August 2009
Unit 11.10 N-Terminal Sequence Analysis of Proteins and Peptides
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Unit 12.1 Overview of Glycoconjugate Analysis
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Unit 12.2 Metabolic Radiolabeling of Animal Cell Glycoconjugates
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Unit 18.2 Combinatorial Peptide Synthesis on a Microchip
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Unit 19.2 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
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Unit 19.20 Strep/FLAG Tandem Affinity Purification (SF-TAP) to Study Protein Interactions
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Supplement 56, April 2009
Unit 2.1 Computational Methods for Protein Sequence Comparison and Search
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Unit 5.23 Autoinduction of Protein Expression
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Unit 15.3 Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation
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Unit 14.4 Analysis of Oxidative Modification of Proteins
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Unit 19.5 Imaging Protein-Protein Interactions by Förster Resonance Energy Transfer (FRET) Microscopy in Live Cells
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Unit 21.19 The CLIP-CHIP Oligonucleotide Microarray: Dedicated Array for Analysis of All Protease, Nonproteolytic Homolog, and Inhibitor Gene Transcripts in Human and Mouse
Abstract | Full Text: HTML PDF
Supplement 55, February 2009
Unit 3.10 Microvolume Spectrophotometric and Fluorometric Determination of Protein Concentration
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Unit 6.9 Overview on Concepts and Applications of Fab Antibody Fragments
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Unit 6.10 E. coli Expression and Purification of Fab Antibody Fragments
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Unit 14.2 Analysis of Protein Acylation
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Unit 14.4 Analysis of Oxidative Modification of Proteins
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Unit 19.19 Tandem Affinity Purification of Proteins
Abstract | Full Text: HTML PDF
Supplement 54, November 2008
Unit 5.22 A Bacterial Cell-Free Expression System to Produce Membrane Proteins and Proteoliposomes: From cDNA to Functional Assay
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Unit 14.11 Analysis of Protein Lysine Acetylation In Vitro and In Vivo
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Unit 14.12 Determination of Protein Lysine Deacetylation
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Unit 18.11 Expression and Purification of ZEBRA Fusion Proteins and Applications for the Delivery of Macromolecules into Mammalian Cells
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Unit 19.18 Detection and Analysis of Protein-Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis
Abstract | Full Text: HTML PDF
Supplement 53, August 2008
Unit 4.8 The Use of Detergents to Purify Membrane Proteins
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Unit 7.12 Determination of Membrane Protein Molecular Weight Using Sedimentation Equilibrium Analytical Ultracentrifugation
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Unit 23.6 Sample Preparation and In-Solution Protease Digestion of Proteins for Chromatography-Based Proteomic Analysis
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Supplement 52, May 2008
Unit 5.21 Recombinant Protein Complex Expression in E. coli
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Unit 6.6 Expression and Purification of GST Fusion Proteins
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Unit 9.3 Affinity Purification of Natural Ligands
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Unit 19.17 Membrane-Based Yeast Two-Hybrid System to Detect Protein Interactions
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Appendix 4K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract | Full Text: HTML PDF
Supplement 51, February 2008
Unit 5.20 MultiBac: Multigene Baculovirus-Based Eukaryotic Protein Complex Production
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Unit 14.10 Analysis of Histone Modifications by Mass Spectrometry
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Unit 14.11 Detection and Analysis of Protein-Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis
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Unit 18.9 Phage Display for Generating Peptide Reagents
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Unit 18.10 Utilizing Peptide SPOT Arrays to Identify Protein Interactions
Abstract | Full Text: HTML PDF
Supplement 50, November 2007
Unit 2.9 Comparative Protein Structure Modeling Using MODELLER
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Unit 2.13 Protein Structure Analysis Online
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Unit 5.19 Overview on the Expression of Toxic Gene Products in Escherichia coli
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Unit 18.8 Overview of Solid Phase Synthesis of "Difficult Peptide" Sequences
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Unit 24.4 Phosphoproteomics
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Supplement 49, August 2007
Unit 19.4 Detection of Protein-Protein Interactions by Coprecipitation
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Unit 21.18 Proteomic Identification of Cellular Protease Substrates Using Isobaric Tags for Relative and Absolute Quantification (iTRAQ)
Abstract | Full Text: HTML PDF
Unit 21.19 The CLIP-CHIP Oligonucleotide Microarray: Dedicated Array for Analysis of All Protease, Nonproteolytic Homolog, and Inhibitor Gene Transcripts in Human and Mouse
Full Text: HTML PDF
Unit 21.20 Purification and Characterization of Gingipains
Abstract | Full Text: HTML PDF
Unit 21.21 Microplate Assay For Cathepsin Detection in Viable Cells Using Derivatives of 4-Methoxy-ß-Naphthylamide
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Unit 25.2 Overview of Tandem Mass Spectrometry (MS/MS) Database Search Algorithms
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Supplement 48, May 2007
Unit 2.12 Minimotif Miner: A Computational Tool to Investigate Protein Function, Disease, and Genetic Diversity
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Unit 3.4 Assays for Determination of Protein Concentration
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Unit 10.6 Protein Detection in Gels Without Fixation
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Unit 24.3 Glycoproteomics Using Chemical Immobilization
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Supplement 47, February 2007
Unit 6.8 Automated Large-Scale Purification of a Recombinant G-Protein-Coupled Neurotensin Receptor
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Unit 17.9 Crystallization of Integral Membrane Proteins
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Unit 19.16 Determination of Protein Contacts by Chemical Cross-Linking With EDC and Mass Spectrometry
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Unit 26.3 Incorporation of Isotopically Enriched Amino Acids
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Supplement 46, November 2006
Unit 14.6 Analysis of Protein S-Nitrosylation
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Unit 14.9 Identifying and Quantifying Sites of Protein Methylation by Heavy Methyl SILAC
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Unit 19.15 Identifying Small-Molecule Modulators of Protein-Protein Interactions
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Unit 20.10 Circular Dichroism to Study Protein Interactions
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Unit 20.11 Quantitative Determination of Protein Stability and Ligand Binding by Pulse Proteolysis
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Appendix 3C Techniques for Mammalian Cell Tissue Culture
Abstract | Full Text: HTML PDF
Supplement 45, August 2006
Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
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Unit 19.12 Fluorescence Quenching Methods to Study Lipid-Protein Interactions
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Unit 19.13 Overview of Biacore Systems and Their Applications
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Unit 19.14 Using Biacore to Measure the Binding Kinetics of an Antibody-Antigen Interaction
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Supplement 44, May 2006
Unit 5.18 Wheat Germ Cell-Free Expression System for Protein Production
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Unit 14.8 Analysis of Protein Sumoylation
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Unit 24.2 Organelle Proteomics
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Unit 26.2 Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras
Abstract | Full Text: HTML PDF
Supplement 43, February 2006
Unit 12.6 Determining the Structure of Oligosaccharides N- and O-Linked to Glycoproteins
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Unit 12.7 Determining the Structure of Glycan Moieties by Mass Spectrometry
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Unit 19.11 Production and Use of Trimeric Isoleucine Zipper Fusion Proteins to Study Surface Receptor Ligand Interactions
Abstract | Full Text: HTML PDF
Supplement 42, November 2005
Unit 17.2 Electron Microscopy and Image Processing: An Essential Tool for Structural Analysis of Macromolecules
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Unit 24.1 Human Serum and Plasma Proteomics
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Unit 25.1 Bioinformatics Analysis for Interactive Proteomics
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Unit 26.1 Misincorporation Proton-Alkyl Exchange (MPAX): Engineering Cysteine Probes into Proteins
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Supplement 41, August 2005
Unit 2.10 Protein Charge Determination
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Unit 2.11 Basic Protein Sequence Analysis
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Unit 6.2 Preparation of Soluble Proteins from Escherichia coli
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Unit 10.12 Overview of Digital Electrophoresis Analysis
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Unit 19.10 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
Abstract | Full Text: HTML PDF
Supplement 40, May 2005
Unit 8.8 Expanded-Bed Adsorption Chromatography
Abstract | Full Text: HTML PDF
Unit 18.7 Guide for Resin and Linker Selection in Solid-Phase Peptide Synthesis
Abstract | Full Text: HTML PDF
Unit 20.9 Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein-Protein Interactions
Abstract | Full Text: HTML PDF
Supplement 39, February 2005
Unit 14.7 Tyrosine O-Sulfation
Abstract | Full Text: HTML PDF
Unit 20.8 Spectroscopic Methods for the Determination of Protein Interactions
Abstract | Full Text: HTML PDF
Unit 22.6 Protein Profiling by Microscale Solution Isoelectrofocusing (MicroSol-IEF)
Abstract | Full Text: HTML PDF
Unit 23.5 Protein Microarrays
Abstract | Full Text: HTML PDF
Supplement 38, November 2004
Unit 4.4 Desalting, Concentration, and Buffer Exchange by Dialysis and Ultrafiltration
Abstract | Full Text: HTML PDF
Unit 4.7 Extraction of Proteins from Plant Tissues
Abstract | Full Text: HTML PDF
Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
Abstract | Full Text: HTML PDF
Unit 7.6 Determining the CD Spectrum of a Protein
Abstract | Full Text: HTML PDF
Unit 7.7 Determining the Fluorescence Spectrum of a Protein
Abstract | Full Text: HTML PDF
Unit 11.3 Enzymatic Digestion of Proteins in Gels for Mass Spectrometric Identification and Structural Analysis
Abstract | Full Text: HTML PDF
Unit 19.9 Identifying Protein Interactions by Hydroxyl-Radical Protein Footprinting
Abstract | Full Text: HTML PDF
Supplement 37, August 2004
Unit 4.1 Overview of Cell Fractionation
Abstract | Full Text: HTML PDF
Unit 4.2 Purification of Organelles from Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 11.11 Determination of Disulfide-Bond Linkages in Proteins
Abstract | Full Text: HTML PDF
Supplement 36, May 2004
Unit 9.9 Overview of Affinity Tags for Protein Purification
Abstract | Full Text: HTML PDF
Unit 14.6 Analysis of Protein S-Nitrosylation
Full Text: HTML PDF
Unit 21.17 Applications for Chemical Probes of Proteolytic Activity
Abstract | Full Text: HTML PDF
Supplement 35, February 2004
Unit 17.1 Overview of Protein Structural and Functional Folds
Abstract | Full Text: HTML PDF
Supplement 34, November 2003
Unit 13.6 Preparation and Application of Polyclonal and Monoclonal Sequence-Specific Anti-Phosphoamino Acid Antibodies
Abstract | Full Text: HTML PDF
Unit 17.4 Crystallization of Macromolecules
Abstract | Full Text: HTML PDF
Unit 23.1 Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry
Abstract | Full Text: HTML PDF
Unit 23.2 Quantitative Protein Profile Comparisons Using the Isotope-Coded Affinity Tag Method
Abstract | Full Text: HTML PDF
Unit 23.3 Proteomic Analysis Using 2-D Liquid Separations of Intact Proteins From Whole-Cell Lysates
Abstract | Full Text: HTML PDF
Unit 23.4 Quantitative Protein Analysis Using Proteolytic [ 18 O]Water Labeling
Abstract | Full Text: HTML PDF
Supplement 33, August 2003
Unit 2.6 Protein Databases on the Internet
Abstract | Full Text: HTML PDF
Unit 3.1 Spectrophotometric Determination of Protein Concentration
Abstract | Full Text: HTML PDF
Unit 17.8 Raman Spectroscopy of Proteins
Abstract | Full Text: HTML PDF
Unit 21.15 Zymography of Metalloproteinases
Abstract | Full Text: HTML PDF
Unit 21.16 Monitoring Metalloproteinase Activity Using Synthetic Fluorogenic Substrates
Abstract | Full Text: HTML PDF
Supplement 32, May 2003
Unit 4.3 Subcellular Fractionation of Tissue Culture Cells
Abstract | Full Text: HTML PDF
Unit 21.14 Expression, Purification, and Characterization of Aspartic Endopeptidases: Plasmodium Plasmepsins and "Short" Recombinant Human Pseudocathepsin
Abstract | Full Text: HTML PDF
Unit 22.4 Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison
Abstract | Full Text: HTML PDF
Unit 22.5 Isolation of Organelles and Prefractionation of Protein Extracts Using Free-Flow Electrophoresis
Abstract | Full Text: HTML PDF
Supplement 31, February 2003
Unit 7.11 Rapid Screening of E. coli Extracts by Heteronuclear NMR
Abstract | Full Text: HTML PDF
Unit 11.8 C-Terminal Sequence Analysis
Abstract | Full Text: HTML PDF
Unit 13.10 Use of Protein Phosphatase Inhibitors
Abstract | Full Text: HTML PDF
Unit 20.7 Analytical Ultracentrifugation: Sedimentation Velocity Analysis
Abstract | Full Text: HTML PDF
Unit 22.3 Laser Capture Microdissection for Proteome Analysis
Abstract | Full Text: HTML PDF
Supplement 30, December 2002
Unit 1.5 Overview of the Physical State of Proteins within Cells
Abstract | Full Text: HTML PDF
Unit 5.17 Use of the Gateway System for Protein Expression in Multiple Hosts
Abstract | Full Text: HTML PDF
Unit 6.1 Overview of the Purification of Recombinant Proteins Produced in Escherichia coli
Abstract | Full Text: HTML PDF
Unit 21.13 Expression, Purification, and Characterization of Caspases
Abstract | Full Text: HTML PDF
Unit 22.1 Overview of Proteome Analysis
Abstract | Full Text: HTML PDF
Unit 22.2 Protein Profiling Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
Abstract | Full Text: HTML PDF
Supplement 29, September 2002
Unit 1.4 Purification of Glutamate Dehydrogenase from Liver and Brain
Abstract | Full Text: HTML PDF
Unit 10.5 Protein Detection in Gels Using Fixation
Abstract | Full Text: HTML PDF
Unit 14.5 Analysis of Protein Ubiquitination
Abstract | Full Text: HTML PDF
Unit 17.7 Introduction to Atomic Force Microscopy (AFM) in Biology
Full Text: HTML PDF
Appendix 4J The Polymerase Chain Reaction
Abstract | Full Text: HTML PDF
Supplement 28, June 2002
Unit 2.9 Comparative Protein Structure Prediction
Full Text: HTML PDF
Unit 17.6 Probing Protein Structure and Dynamics by Hydrogen Exchange–Mass Spectrometry
Full Text: HTML PDF
Unit 18.3 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
Abstract | Full Text: HTML PDF
Appendix 2A Laboratory Safety
Abstract | Full Text: HTML PDF
Supplement 27, March 2002
Unit 4.6 Long-Term Storage of Proteins
Abstract | Full Text: HTML PDF
Unit 16.11 De Novo Peptide Sequencing via Manual Interpretation of MS/MS Spectra
Abstract | Full Text: HTML PDF
Unit 19.8 Scintillation Proximity Assay ( SPA ) Technology to Study Biomolecular Interactions
Abstract | Full Text: HTML PDF
Unit 21.11 Over-Expression and Purification of Active Serine Proteases and Their Variants from Escherichia coli Inclusion Bodies
Abstract | Full Text: HTML PDF
Unit 21.12 Assaying Proteases in Cellular Environments
Abstract | Full Text: HTML PDF
Supplement 26, December 2001
Unit 3.1 Spectrophotometric Determination of Protein Concentration
Unit 3.9 Solid-Phase Profiling of Proteins
Abstract | Full Text: HTML PDF
Unit 18.1 Introduction to Peptide Synthesis
Abstract | Full Text: HTML PDF
Unit 21.7 Serpins (Serine Protease Inhibitors)
Abstract | Full Text: HTML PDF
Unit 21.8 Caspases
Abstract | Full Text: HTML PDF
Unit 21.9 Use of GFP as a Reporter for the Analysis of Sequence-Specific Proteases
Abstract | Full Text: HTML PDF
Unit 21.10 An Overview of Serine Proteases
Abstract | Full Text: HTML PDF
Supplement 25, September 2001
Unit 12.8 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
Abstract | Full Text: HTML PDF
Unit 19.7 Identification of Protein Interactions by Far Western Analysis
Abstract | Full Text: HTML PDF
Unit 20.6 Size-Exclusion Chromatography with On-Line Light Scattering
Abstract | Full Text: HTML PDF
Unit 21.6 Purification of the Eukaryotic 20S Proteasome
Abstract | Full Text: HTML PDF
Supplement 24, June 2001
Unit 11.6 Reversed-Phase Isolation of Peptides
Abstract | Full Text: HTML PDF
Unit 19.6 High-Throughput Screening for Protein-Protein Interactions Using Yeast Two-Hybrid Arrays
Abstract | Full Text: HTML PDF
Unit 21.4 Metalloproteases
Abstract | Full Text: HTML PDF
Unit 21.5 Purification and Characterization of Proteasomes from Saccharomyces cerevisiae
Abstract | Full Text: HTML PDF
Supplement 23, March 2001
Unit 8.7 HPLC of Peptides and Proteins
Abstract | Full Text: HTML PDF
Unit 18.6 Disulfide Bond Formation in Peptides
Abstract | Full Text: HTML PDF
Unit 19.5 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
Full Text: HTML PDF
Unit 21.3 Overview of Pepsin-like Aspartic Peptidases
Abstract | Full Text: HTML PDF
Supplement 22, December 2000
Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
Full Text: HTML PDF
Unit 12.6 Determining the Structure of Oligosaccharides N- and O-Linked to Glycoproteins
Full Text: HTML PDF
Unit 12.7 Determining the Structure of Glycan Moieties by Mass Spectrometry
Full Text: HTML PDF
Unit 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface
Abstract | Full Text: HTML PDF
Unit 16.9 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using Microscale Capillary Liquid Chromatography
Abstract | Full Text: HTML PDF
Unit 16.10 Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching
Abstract | Full Text: HTML PDF
Supplement 21, September 2000
Unit 21.1 Proteases
Abstract | Full Text: HTML PDF
Unit 21.2 Papain-like Cysteine Proteases
Abstract | Full Text: HTML PDF
Appendix 3G Statistics: Detecting Differences Among Groups
Abstract | Full Text: HTML PDF
Appendix 3H Analyzing Radioligand Binding Data
Abstract | Full Text: HTML PDF
Supplement 20, June 2000
Unit 2.4 Internet Basics
Abstract | Full Text: HTML PDF
Unit 3.8 Analysis of Selenocysteine-Containing Proteins
Abstract | Full Text: HTML PDF
Unit 5.16 Choice of Cellular Protein Expression System
Abstract | Full Text: HTML PDF
Unit 14.4 Analysis of Oxidative Modification of Proteins
Full Text: HTML PDF
Supplement 19, March 2000
Unit 2.6 Protein Databases on the Internet
Unit 2.7 Protein Tertiary Structure Prediction
Abstract | Full Text: HTML PDF
Unit 2.8 Protein Tertiary Structure Modeling
Abstract | Full Text: HTML PDF
Unit 11.4 Chemical Cleavage of Proteins in Solution
Abstract | Full Text: HTML PDF
Unit 11.5 Chemical Cleavage of Proteins on Membranes
Abstract | Full Text: HTML PDF
Unit 17.5 Introduction to NMR of Proteins
Abstract | Full Text: HTML PDF
Supplement 18, December 1999
Unit 9.8 Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 13.9 Phosphopeptide Mapping and Identification of Phosphorylation Sites
Abstract | Full Text: HTML PDF
Unit 20.3 Analytical Centrifugation: Equilibrium Approach
Abstract | Full Text: HTML PDF
Unit 20.4 Titration Microcalorimetry
Abstract | Full Text: HTML PDF
Unit 20.5 Reduced-Scale Large-Zone Analytical Gel-Filtration Chromatography for Measurement of Protein Association Equilibria
Abstract | Full Text: HTML PDF
Supplement 17, September 1999
Unit 3.7 Metabolic Labeling with Amino Acids
Abstract | Full Text: HTML PDF
Unit 18.5 Synthesis and Application of Peptide Dendrimers As Protein Mimetics
Abstract | Full Text: HTML PDF
Unit 19.4 Detection of Protein-Protein Interactions by Coprecipitation
Full Text: HTML PDF
Unit 20.1 Overview of the Quantitation of Protein Interactions
Abstract | Full Text: HTML PDF
Unit 20.2 Measuring Protein Interactions by Optical Biosensors
Abstract | Full Text: HTML PDF
Appendix 1D Common Conversion Factors
Abstract | Full Text: HTML PDF
Supplement 16, June 1999
Unit 7.10 Characterizing Recombinant Proteins Using HPLC Gel Filtration and Mass Spectrometry
Abstract | Full Text: HTML PDF
Unit 10.13 Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers
Abstract | Full Text: HTML PDF
Appendix 5A Theoretical Aspects of the Quantitative Characterization of Ligand Binding
Abstract | Full Text: HTML PDF
Supplement 15, March 1999
Unit 2.5 Sequence Similarity Searching Using the BLAST Family of Programs
Abstract | Full Text: HTML PDF
Unit 8.2 Ion-Exchange Chromatography
Abstract | Full Text: HTML PDF
Unit 16.7 Enzymatic Approaches for Obtaining Amino Acid Sequence: On-Target Ladder Sequencing
Abstract | Full Text: HTML PDF
Unit 18.4 Native Chemical Ligation of Polypeptides
Abstract | Full Text: HTML PDF
Unit 19.3 Phage-Based Expression Cloning to Identify Interacting Proteins
Abstract | Full Text: HTML PDF
Supplement 14, December 1998
Unit 16.3 Sample Preparation for MALDI Mass Analysis of Peptides and Proteins
Abstract | Full Text: HTML PDF
Unit 16.4 In-Gel Digestion of Proteins for MALDI-MS Fingerprint Mapping
Abstract | Full Text: HTML PDF
Unit 16.5 Searching Sequence Databases Over the Internet: Protein Identification Using MS-Fit
Abstract | Full Text: HTML PDF
Unit 16.6 Searching Sequence Databases Over the Internet: Protein Identification Using MS-Tag
Abstract | Full Text: HTML PDF
Unit 19.1 Analysis of Protein-Protein Interactions
Abstract | Full Text: HTML PDF
Unit 19.2 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Full Text: HTML PDF
Appendix 4L Growth and Manipulation of Yeast
Abstract | Full Text: HTML PDF
Supplement 13, September 1998
Unit 5.11 Overview of the Vaccinia Virus Expression System
Abstract | Full Text: HTML PDF
Unit 5.12 Preparation of Cell Cultures and Vaccinia Virus Stocks
Abstract | Full Text: HTML PDF
Unit 5.13 Generation of Recombinant Vaccinia Viruses
Abstract | Full Text: HTML PDF
Unit 5.14 Characterization of Recombinant Vaccinia Viruses and Their Products
Abstract | Full Text: HTML PDF
Unit 5.15 Gene Expression Using the Vaccinia Virus/ T7 RNA Polymerase Hybrid System
Abstract | Full Text: HTML PDF
Appendix 3E Silanizing Glassware
Abstract | Full Text: HTML PDF
Appendix 3F Protein Precipitation Using Ammonium Sulfate
Abstract | Full Text: HTML PDF
Appendix 4 Molecular Biology Techniques
Abstract | Full Text: HTML
Appendix 4A Media Preparation and Bacteriological Tools
Abstract | Full Text: HTML PDF
Appendix 4B Growth in Liquid or Solid Media
Abstract | Full Text: HTML PDF
Appendix 4C Preparation of Plasmid DNA
Abstract | Full Text: HTML PDF
Appendix 4D Introduction of Plasmid DNA into Cells
Abstract | Full Text: HTML PDF
Appendix 4E Purification and Concentration of DNA from Aqueous Solutions
Abstract | Full Text: HTML PDF
Appendix 4F Agarose Gel Electrophoresis
Abstract | Full Text: HTML PDF
Appendix 4G Southern Blotting
Abstract | Full Text: HTML PDF
Appendix 4H Hybridization Analysis of DNA Blots
Abstract | Full Text: HTML PDF
Appendix 4I Digestion of DNA with Restriction Endonucleases
Abstract | Full Text: HTML PDF
Appendix 4K Quantitation of Nucleic Acids with Absorption Spectroscopy
Full Text: HTML PDF
Supplement 12, June 1998
Unit 5.10 Production of Recombinant Proteins in Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 7.9 Measuring Protein Thermostability by Differential Scanning Calorimetry
Abstract | Full Text: HTML PDF
Unit 9.7 Purification of DNA -Binding Proteins Using Biotin/Streptavidin Affinity Systems
Abstract | Full Text: HTML PDF
Unit 17.4 Crystallization of Soluble Macromolecules
Supplement 11, February 1998
Unit 2.4 Internet Basics
Unit 7.8 Light Scattering
Abstract | Full Text: HTML PDF
Unit 9.6 Purification of Sequence-Specific DNA -Binding Proteins by Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 10.12 Digital Electrophoresis Analysis
Full Text: HTML PDF
Appendix 1B Commonly Used Detergents
Abstract | Full Text: HTML PDF
Appendix 1C Conversion Factors and Half-Life Information for Radioactivity
Abstract | Full Text: HTML PDF
Supplement 10, December 1997
Unit 5.9 Overview of Protein Expression by Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 6.7 Expression and Purification of Thioredoxin Fusion Proteins
Abstract | Full Text: HTML PDF
Unit 10.11 Autoradiography
Abstract | Full Text: HTML PDF
Unit 13.2 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 13.8 Permeabilization Strategies to Study Protein Phosphorylation
Abstract | Full Text: HTML PDF
Unit 17.3 Principles of Macromolecular X-Ray Crystallography
Abstract | Full Text: HTML PDF
Unit 18.3 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
Supplement 9, September 1997
Unit 6.6 Expression and Purification of GST Fusion Proteins
Full Text: HTML PDF
Unit 13.7 Assays of Protein Kinases Using Exogenous Substrates
Abstract | Full Text: HTML PDF
Unit 17.2 Overview of Macromolecular Electron Microscopy: An Essential Tool in Protein Structural Analysis
Full Text: HTML PDF
Unit 18.1 Introduction to Peptide Synthesis
Unit 18.2 Synthesis of Multiple Peptides on Plastic Pins
Full Text: HTML PDF
Supplement 8, June 1997
Unit 8.6 Hydroxylapatite Chromatography
Abstract | Full Text: HTML PDF
Unit 11.10 N-Terminal Sequence Analysis of Proteins and Peptides
Full Text: HTML PDF
Unit 17.1 Overview of Protein Structural and Functional Folds
Supplement 7, March 1997
Unit 4.5 Selective Precipitation of Proteins
Abstract | Full Text: HTML PDF
Unit 11.6 Reversed-Phase Isolation of Peptides
Unit 11.9 Amino Acid Analysis
Full Text: HTML PDF
Appendix 3C Techniques for Mammalian Cell Tissue Culture
Full Text: HTML PDF
Appendix 3D Importing Biological Materials
Abstract | Full Text: HTML PDF
Supplement 6, December 1996
Unit 2.4 Internet Basics
Unit 2.5 Sequence Similarity Searching Using the BLAST Family of Programs
Unit 3.6 Biotinylation of Proteins in Solution and on Cell Surfaces
Full Text: HTML PDF
Unit 7.7 Determining the Fluorescence Spectrum of a Protein
Full Text: HTML PDF
Supplement 5, September 1996
Unit 3.5 Kinetic Assay Methods
Abstract | Full Text: HTML PDF
Unit 7.6 Determining the CD Spectrum of a Protein
Full Text: HTML PDF
Unit 13.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides
Unit 14.2 Analysis of Protein Acylation
Full Text: HTML PDF
Unit 14.3 Analysis of Protein Prenylation and Carboxyl-Methylation
Abstract | Full Text: HTML PDF
Supplement 4, June 1996
Unit 7.5 Analytical Ultracentrifugation
Abstract | Full Text: HTML PDF
Unit 10.10 Immunoblot Detection
Abstract | Full Text: HTML PDF
Unit 11.8 C-Terminal Sequence Analysis
Unit 15.2 Modification of Amino Groups
Abstract | Full Text: HTML PDF
Unit 16.1 Overview of Peptide and Protein Analysis by Mass Spectrometry
Abstract | Full Text: HTML PDF
Unit 16.2 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides
Abstract | Full Text: HTML PDF
Supplement 3, March 1996
Unit 7.3 Determining the Identity and Structure of Recombinant Proteins
Abstract | Full Text: HTML PDF
Unit 7.4 Transverse Urea-Gradient Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 9.5 Immunoaffinity Chromatography
Abstract | Full Text: HTML PDF
Unit 11.7 Removal of N-Terminal Blocking Groups from Proteins
Abstract | Full Text: HTML PDF
Unit 14.1 Analysis of Disulfide Bond Formation
Abstract | Full Text: HTML PDF
Unit 15.1 Modification of Cysteine
Abstract | Full Text: HTML PDF
Supplement 2, December 1995
Unit 4.4 Concentration, Desalting, and Buffer Exchange
Unit 5.6 Overview of Protein Expression in Saccharomyces cerevisiae
Abstract | Full Text: HTML PDF
Unit 5.7 Overview of Protein Expression in Pichia pastoris
Abstract | Full Text: HTML PDF
Unit 5.8 Culture of Yeast for the Production of Heterologous Proteins
Abstract | Full Text: HTML PDF
Unit 8.5 Chromatofocusing
Abstract | Full Text: HTML PDF
Unit 10.9 Capillary Electrophoresis of Proteins and Peptides
Abstract | Full Text: HTML PDF
Unit 12.5 Detection of Glycophospholipid Anchors on Proteins
Abstract | Full Text: HTML PDF
Supplement 1, September 1995
Unit 3.4 Assays for Total Protein
Full Text: HTML PDF
Unit 4.3 Subcellular Fractionation
Unit 7.2 Determining the Identity and Purity of Recombinant Proteins by UV Absorption Spectroscopy
Abstract | Full Text: HTML PDF
Unit 9.4 Metal-Chelate Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 12.4 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
Core Publication, June 1995
Unit 1.1 Overview of Protein Purification and Characterization
Abstract | Full Text: HTML PDF
Unit 1.2 Strategies for Protein Purification
Abstract | Full Text: HTML PDF
Unit 1.3 Protein Purification Flow Charts
Abstract | Full Text: HTML PDF
Unit 2.1 Computational Methods for Protein Sequence Analysis
Full Text: HTML PDF
Unit 2.2 Hydrophobicity Profiles for Protein Sequence Analysis
Abstract | Full Text: HTML PDF
Unit 2.3 Protein Secondary Structure Prediction
Abstract | Full Text: HTML PDF
Unit 3.1 Spectrophotometric Determination of Protein Concentration
Unit 3.2 Quantitative Amino Acid Analysis
Abstract | Full Text: HTML PDF
Unit 3.3 In Vitro Radiolabeling of Peptides and Proteins
Abstract | Full Text: HTML PDF
Unit 4.1 Overview of Cell Fractionation
Unit 4.2 Purification of Organelles from Mammalian Cells
Unit 5.1 Production of Recombinant Proteins in Escherichia coli
Abstract | Full Text: HTML PDF
Unit 5.2 Selection of Escherichia coli Expression Systems
Abstract | Full Text: HTML PDF
Unit 5.3 Fermentation and Growth of Escherichia coli for Optimal Protein Production
Abstract | Full Text: HTML PDF
Unit 5.4 Overview of the Baculovirus Expression System
Abstract | Full Text: HTML PDF
Unit 5.5 Protein Expression in the Baculovirus System
Abstract | Full Text: HTML PDF
Unit 6.1 Overview of the Purification of Recombinant Proteins Produced in Escherichia coli
Unit 6.2 Preparation of Soluble Proteins from Escherichia coli
Unit 6.3 Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
Unit 6.4 Overview of Protein Folding
Abstract | Full Text: HTML PDF
Unit 6.5 Folding and Purification of Insoluble (Inclusion Body) Proteins from Escherichia coli
Abstract | Full Text: HTML PDF
Unit 7.1 Overview of the Characterization of Recombinant Proteins
Abstract | Full Text: HTML PDF
Unit 8.1 Overview of Conventional Chromatography
Abstract | Full Text: HTML PDF
Unit 8.2 Ion-Exchange Chromatography
Unit 8.3 Gel-Filtration Chromatography
Abstract | Full Text: HTML PDF
Unit 8.4 Hydrophobic-Interaction Chromatography
Abstract | Full Text: HTML PDF
Unit 9.1 Lectin Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 9.2 Dye Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 9.3 Affinity Purification of Natural Ligands
Full Text: HTML PDF
Unit 10.1 One-Dimensional SDS Gel Electrophoresis of Proteins
Abstract | Full Text: HTML PDF
Unit 10.2 One-Dimensional Isoelectric Focusing of Proteins in Slab Gels
Abstract | Full Text: HTML PDF
Unit 10.3 One-Dimensional Electrophoresis Using Nondenaturing Conditions
Abstract | Full Text: HTML PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis
Unit 10.5 Protein Detection in Gels Using Fixation
Unit 10.6 Protein Detection in Gels Without Fixation
Unit 10.7 Electroblotting from Polyacrylamide Gels
Abstract | Full Text: HTML PDF
Unit 10.8 Detection of Proteins on Blot Membranes
Abstract | Full Text: HTML PDF
Unit 11.1 Enzymatic Digestion of Proteins in Solution
Abstract | Full Text: HTML PDF
Unit 11.2 Enzymatic Digestion of Proteins on PVDF Membranes
Abstract | Full Text: HTML PDF
Unit 11.3 Enzymatic Digestion of Proteins in Gels for Mass Spectrometric Identification and Structural Analysis
Unit 11.4 Chemical Cleavage of Proteins in Solution
Unit 11.5 Chemical Cleavage of Proteins on Membranes
Unit 11.6 Reversed-Phase Isolation of Peptides
Unit 12.1 Overview of Glycoconjugate Analysis
Full Text: HTML PDF
Unit 12.2 Metabolic Radiolabeling of Animal Cell Glycoconjugates
Full Text: HTML PDF
Unit 12.3 Inhibition of N-Linked Glycosylation
Abstract | Full Text: HTML PDF
Unit 13.1 Overview of Protein Phosphorylation
Abstract | Full Text: HTML PDF
Unit 13.2 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation
Unit 13.3 Phosphoamino Acid Analysis
Abstract | Full Text: HTML PDF
Unit 13.4 Detection of Phosphorylation by Immunological Techniques
Abstract | Full Text: HTML PDF
Unit 13.5 Detection of Phosphorylation by Enzymatic Techniques
Abstract | Full Text: HTML PDF
Appendix 1A Characteristics of Amino Acids
Abstract | Full Text: HTML PDF
Appendix 2A Laboratory Safety
Appendix 2B Safe Use of Radioisotopes
Abstract | Full Text: HTML PDF
Appendix 2C Centrifuges and Rotors
Abstract | Full Text: HTML PDF
Appendix 2D Standard Laboratory Equipment
Abstract | Full Text: HTML PDF
Appendix 2E Commonly Used Reagents
Abstract | Full Text: HTML PDF
Appendix 3A Use of Protein Folding Reagents
Abstract | Full Text: HTML PDF
Appendix 3B Dialysis
Abstract | Full Text: HTML PDF
