Current Protocols in Protein Science

Mission Statement

Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.

Current Protocols in Protein Science is the comprehensive resource for the experimental investigation of protein structure and function. Current Protocols in Protein Science brings together hundreds of core and cutting edge techniques in all aspects of protein science, including production, purification and characterization of proteins from natural and recombinant sources, mass spectrometry of proteins and peptides, electrophoretic separations, chromatography, characterization of post-translational modifications, preparation and handling of peptides, structural biology, protein interactions, protein engineering, computational analyses and proteomics. Current Protocols in Protein Science also includes valuable appendices, providing basic methodologies such as molecular biology techniques, cell culture methods, and biophysical data analysis. Updated quarterly, Current Protocols in Protein Science constantly evolves to keep pace with the newest discoveries and developments.

The target audience is both the novice and the experienced experimenter—the first-year graduate student or new post-doctoral fellow and the seasoned investigator who wants to branch out into a new area of research. Sufficient detail is provided to permit duplication of the protocols in any laboratory, whatever the disciplinary background or level of sophis¬tication.

Readership

protein biochemists • chemical biologists • structural biologists • mass spectrometrists • chemists • molecular biologists • microbiologists • plant scientists • geneticists • bioinformaticians

Keywords

Proteins, protein structure, protein purification, protein expression, structural biology, proteomics, post-translational modifications, protein interactions, protein characterization, mass spectrometry, protein-ligand interactions, proteases, peptides, protein engineering, laboratory protocols, Current Protocols