Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

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  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
    1. Adhesion
    2. Analysis of Proteins
      1. Analysis of Protein Ligand-Receptor Binding by Photoaffinity Cross-Linking
      2. Analysis of Protein Prenylation In Vitro and In Vivo Using Functionalized Phosphoisoprenoids
      3. Analysis of Protein Sumoylation
      4. Analysis of Protein Ubiquitination
      5. Analytical Ultracentrifugation
      6. Autoradiography
      7. Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers
      8. Capillary Electrophoresis of Proteins and Peptides
      9. Characterization of Protein Content Present in Exosomes Isolated from Conditioned Media and Urine
      10. Characterizing Recombinant Proteins Using HPLC Gel Filtration and Mass Spectrometry
      11. Computational Prediction of Protein Secondary Structure from Sequence
      12. Crystallization of Macromolecules
      13. Detection and Measurement of Methionine Oxidation in Proteins
      14. Detection of Tyrosine Sulfation on Proteins
      15. Determination of Membrane Protein Molecular Weight Using Sedimentation Equilibrium Analytical Ultracentrifugation
      16. Determining the CD Spectrum of a Protein
      17. Determining the Fluorescence Spectrum of a Protein
      18. Determining the Identity and Purity of Recombinant Proteins by UV Absorption Spectroscopy
      19. Determining the Identity and Structure of Recombinant Proteins
      20. Electroblotting from Polyacrylamide Gels
      21. Fast Relaxation Imaging in Living Cells
      22. Fluorescent Labeling of Specific Cysteine Residues Using CyMPL
      23. Handling Metalloproteinases
      24. Hydroxylapatite Chromatography
      25. Immunoblotting and Immunodetection
      26. Introduction to Atomic Force Microscopy (AFM) in Biology
      27. Ion-Exchange Chromatography
      28. Light Scattering
      29. Measuring Protein Thermostability by Differential Scanning Calorimetry
      30. Metabolic Labeling with Amino Acids
      31. Metal-Chelate Affinity Chromatography
      32. Modification of Cysteine
      33. Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy
      34. Non-Gradient Blue Native Polyacrylamide Gel Electrophoresis
      35. Nonradioactive Analysis of Dynamic Protein Palmitoylation
      36. One-Dimensional Electrophoresis Using Nondenaturing Conditions
      37. One-Dimensional Isoelectric Focusing of Proteins in Slab Gels
      38. Overview of Conventional Chromatography
      39. Overview of Digital Electrophoresis Analysis
      40. Overview of Protein Microarrays
      41. Overview of Protein Purification and Characterization
      42. Overview of the Characterization of Recombinant Proteins
      43. Overview of the Physical State of Proteins within Cells
      44. Overview of the Purification of Recombinant Proteins
      45. Preparation of Recombinant Protein Spotted Arrays for Proteome-Wide Identification of Kinase Targets
      46. Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison
      47. Protein Detection in Gels Using Fixation
      48. Protein Detection in Gels Without Fixation
      49. Protein Purification Flow Charts
      50. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
      51. Pulse-Chase Analysis for Studying Protein Synthesis and Maturation
      52. Quantitative Analysis of Surface Expression of Membrane Proteins Using Cold-Adapted Proteases
      53. Rapid Screening of E. coli Extracts by Heteronuclear NMR
      54. Removal of N-Terminal Blocking Groups from Proteins
      55. Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Bound Protein Clusters
      56. Site-Specific Protein Labeling with SNAP-Tags
      57. Strategies for Protein Purification
      58. Transverse Urea-Gradient Gel Electrophoresis
      59. Two-Dimensional Gel Electrophoresis
      60. Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
      61. Enrichment and Detection of Tyrosine-Nitrated Proteins
    3. Antibodies as Tools
    4. Cell Isolation and Culture
    5. Enzyme Assays
    6. Extracellular Matrix
    7. Fluorescent Proteins as Probes of Structure and Function
    8. Labeling Molecules
    9. Lipids and Fatty Acids
    10. Manipulating Gene Expression
    11. Microscopy and Imaging
    12. Molecular Biology Techniques
    13. Molecular Interactions
    14. Molecular Trafficking
    15. Organelle Analysis
    16. Subcellular Fractionation
    17. Whole Organism and Tissue Analysis
  4. Cell Culture
  5. Chemical Biology
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Molecular Biology
  14. Neuroscience
  15. Nucleic Acid Chemistry
  16. Pharmacology and Drug Discovery
  17. Protein Production, Purification, and Analysis
  18. Proteomics
  19. RNA
  20. Safety
  21. Structural Analysis of Biomolecules
  22. Supporting Lab Techniques