Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Gene Expression
  6. Genetics and Genomics
  7. Imaging and Microscopy
  8. Immunology
  9. Intermolecular Interactions
  10. Laboratory Organisms and Animal Models
  11. Microbiology
  12. Molecular Biology
  13. Neuroscience
  14. Nucleic Acid Chemistry
  15. Pharmacology and Drug Discovery
  16. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
      1. Amino Acid Analysis
      2. Analysis of Disulfide Bond Formation
      3. Analysis of Histone Modifications by Mass Spectrometry
      4. Analysis of Oxidative Modification of Proteins
      5. Analysis of Protein Acylation
      6. Analysis of Protein Lysine Acetylation In Vitro and In Vivo
      7. Analysis of Selenocysteine-Containing Proteins
      8. Analytical Centrifugation: Equilibrium Approach
      9. Analytical Ultracentrifugation: Sedimentation Velocity Analysis
      10. Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance
      11. Assays for Determination of Protein Concentration
      12. C-Terminal Sequence Analysis
      13. Circular Dichroism to Study Protein Interactions
      14. Characterization of Recombinant Vaccinia Viruses and Their Products
      15. Detection of Glycophospholipid Anchors on Proteins
      16. Detection of Phosphorylation by Enzymatic Techniques
      17. Detection of Phosphorylation by Immunological Techniques
      18. Detection of Protein-Protein Interactions by Coprecipitation
      19. Detection of Proteins on Blot Membranes
      20. Detection of Tyrosine Sulfation on Proteins
      21. Detergent Analysis in Protein Samples Using Mid-Infrared (MIR) Spectroscopy
      22. Determination of Disulfide-Bond Linkages in Proteins
      23. Determination of Protein Lysine Deacetylation
      24. Determining the CD Spectrum of a Protein
      25. Determining the Fluorescence Spectrum of a Protein
      26. Determining the Identity and Purity of Recombinant Proteins by UV Absorption Spectroscopy
      27. Determining the Identity and Structure of Recombinant Proteins
      28. Determining the Structure of Glycan Moieties by Mass Spectrometry
      29. Application of Dynamic Light Scattering in Protein Crystallization
      30. Electroblotting from Polyacrylamide Gels
      31. Fast Relaxation Imaging in Living Cells
      32. General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays
      33. Handling Metalloproteinases
      34. HPLC-SEC Characterization of Membrane Protein-Detergent Complexes
      35. Immunoblot Detection
      36. Immunoprecipitation
      37. In Vitro Radiolabeling of Peptides and Proteins
      38. In-Gel Phosphatase Assay Using Fluorogenic and Radioactive Substrates
      39. Inhibition of N-Linked Glycosylation
      40. Kinetic Assay Methods
      41. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
      42. Metabolic Labeling with Amino Acids
      43. Microvolume Spectrophotometric and Fluorometric Determination of Protein Concentration
      44. Modification of Amino Groups
      45. Modification of Cysteine
      46. N-Terminal Sequence Analysis of Proteins and Peptides
      47. Native Mass Spectrometry as a Tool in Structural Biology
      48. Determining the Structure of Oligosaccharides N- and O-Linked to Glycoproteins
      49. One-Dimensional SDS Gel Electrophoresis of Proteins
      50. Overview of Protein Phosphorylation
      51. Overview of the Quantitation of Protein Interactions
      52. Permeabilization Strategies to Study Protein Phosphorylation
      53. Phosphoamino Acid Analysis
      54. Phosphopeptide Mapping and Identification of Phosphorylation Sites
      55. Protein Biotinylation
      56. Identifying and Quantifying Sites of Protein Methylation by Heavy Methyl SILAC
      57. Preparation and Analysis of Proteins and Peptides Using MALDI TOF/TOF Mass Spectrometry
      58. Proteolytic Fingerprinting of Complex Biological Samples Using Combinatorial Libraries of Fluorogenic Probes
      59. Quantitative Amino Acid Analysis
      60. Radioligand Binding Analysis as a Tool for Quality Control of GPCR Production for Structural Characterization: Adenosine A2aR as a Template for Study
      61. Rapid Detergent Removal from Peptide Samples with Ethyl Acetate for Mass Spectrometry Analysis
      62. Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System
      63. Removal of N-Terminal Blocking Groups from Proteins
      64. Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
      65. Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins
      66. Spectrophotometric Determination of Protein Concentration
      67. Tetra Detector Analysis of Membrane Proteins
      68. The Use of Detergents to Purify Membrane Proteins
      69. Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
      70. Use of Protein Phosphatase Inhibitors
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptidases and Proteases
    7. Peptides
    8. Production of Recombinant Proteins
    9. Protein Engineering
    10. Purification of Recombinant Proteins
  17. Proteomics
  18. RNA
  19. Safety
  20. Structural Analysis of Biomolecules
  21. Supporting Lab Techniques