Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

Browse by Topic

  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Molecular Biology
  14. Neuroscience
  15. Nucleic Acid Chemistry
  16. Pharmacology and Drug Discovery
  17. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptidases and Proteases
      1. An Overview of Serine Proteases
      2. Applications for Chemical Probes of Proteolytic Activity
      3. Assaying Proteases in Cellular Environments
      4. Caspases
      5. Microplate Assay For Cathepsin Detection in Viable Cells Using Derivatives of 4-Methoxy-β-Naphthylamide
      6. Enzymatic Digestion of Proteins in Gels for Mass Spectrometric Identification and Structural Analysis
      7. Enzymatic Digestion of Proteins in Solution
      8. Enzymatic Digestion of Proteins on PVDF Membranes
      9. Expression, Purification, and Characterization of Aspartic Endopeptidases: Plasmodium Plasmepsins and “Short” Recombinant Human Pseudocathepsin
      10. Expression, Purification, and Characterization of Caspases
      11. Handling Metalloproteinases
      12. Introduction to Peptide Synthesis
      13. Metalloproteases
      14. Over-Expression and Purification of Active Serine Proteases and Their Variants from Escherichia coli Inclusion Bodies
      15. Overview of Pepsin-like Aspartic Peptidases
      16. Papain-like Cysteine Proteases
      17. Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins
      18. Proteases
      19. Quantitative Determination of Protein Stability and Ligand Binding by Pulse Proteolysis
      20. Identification of Protein-Protein Interactions by Surface Plasmon Resonance followed by Mass Spectrometry
      21. Proteolytic Fingerprinting of Complex Biological Samples Using Combinatorial Libraries of Fluorogenic Probes
      22. Proteomic Identification of Cellular Protease Substrates Using Isobaric Tags for Relative and Absolute Quantification (iTRAQ)
      23. Pulsed EPR Distance Measurements in Soluble Proteins by Site-Directed Spin Labeling (SDSL)
      24. Purification and Characterization of Gingipains
      25. Purification and Characterization of Proteasomes from Saccharomyces cerevisiae
      26. Purification of the Eukaryotic 20S Proteasome
      27. Endoglycosidase and Glycoamidase Release of N-Linked Glycans
      28. Separation of Peptides on HALO 2-Micron Particles
      29. Serpins (Serine Protease Inhibitors)
      30. The CLIP-CHIP Oligonucleotide Microarray: Dedicated Array for Analysis of All Protease, Nonproteolytic Homolog, and Inhibitor Gene Transcripts in Human and Mouse
      31. Use of GFP as a Reporter for the Analysis of Sequence-Specific Proteases
      32. Zymography of Metalloproteinases
    7. Peptides
    8. Production of Recombinant Proteins
    9. Protein Engineering
    10. Purification of Recombinant Proteins
  18. Proteomics
  19. RNA
  20. Safety
  21. Structural Analysis of Biomolecules
  22. Supporting Lab Techniques