Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

Browse by Topic

  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Molecular Biology
  14. Neuroscience
  15. Nucleic Acid Chemistry
  16. Pharmacology and Drug Discovery
  17. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptidases and Proteases
    7. Peptides
    8. Production of Recombinant Proteins
    9. Protein Engineering
    10. Purification of Recombinant Proteins
      1. Automated Large-Scale Purification of a Recombinant G-Protein-Coupled Neurotensin Receptor
      2. Displacement Chromatography of Proteins
      3. E. coli Expression and Purification of Fab Antibody Fragments
      4. Elastin-Like Polypeptides as a Purification Tag for Recombinant Proteins
      5. Expression and Purification of GST Fusion Proteins
      6. Expression and Purification of Haemophilus influenzae Rhomboid Intramembrane Protease GlpG for Structural Studies
      7. Expression and Purification of Thioredoxin Fusion Proteins
      8. Folding and Purification of Insoluble (Inclusion Body) Proteins from Escherichia coli
      9. Handling Metalloproteinases
      10. High-Throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
      11. HPLC-SEC Characterization of Membrane Protein-Detergent Complexes
      12. Introduction to Membrane Proteins
      13. Isolation of Monoclonal Antibody Charge Variants by Displacement Chromatography
      14. Metal-Chelate Affinity Chromatography
      15. Obtaining Soluble Folded Proteins from Inclusion Bodies Using Sarkosyl, Triton X-100, and CHAPS: Application to LB and M9 Minimal Media
      16. Strategies to Optimize Protein Expression in E. coli
      17. Overexpression of Membrane Proteins Using Pichia pastoris
      18. Overview of the Purification of Recombinant Proteins
      19. Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
      20. Preparation of Soluble Proteins from Escherichia coli
      21. Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins
      22. Protein Precipitation Using Ammonium Sulfate
      23. Protein Purification Using PDZ Affinity Chromatography
      24. Purification of the Human G Protein−Coupled Receptor Adenosine A2aR in a Stable and Functional Form Expressed in Pichia pastoris
      25. Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System
      26. Reversed-Phase High Performance Liquid Chromatography of Proteins
      27. Selective Precipitation of Proteins
      28. Recombinant Protein Purification by Self-Cleaving Elastin-like Polypeptide Fusion Tag
      29. Tandem Affinity Purification of Proteins
      30. The Use of Detergents to Purify Membrane Proteins
      31. Use of Protein Folding Reagents
  18. Proteomics
  19. RNA
  20. Safety
  21. Structural Analysis of Biomolecules
  22. Supporting Lab Techniques