Current Protocols in Protein Science

Current Protocols in Protein Science

Online ISBN: 9780471140863

DOI: 10.1002/0471140864

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Gene Expression
  6. Genetics and Genomics
  7. Imaging and Microscopy
  8. Immunology
  9. Intermolecular Interactions
  10. Laboratory Organisms and Animal Models
  11. Microbiology
  12. Molecular Biology
  13. Neuroscience
  14. Nucleic Acid Chemistry
  15. Pharmacology and Drug Discovery
  16. Protein Production, Purification, and Analysis
  17. Proteomics
    1. Mass Spectrometry
      1. Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry
      2. Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
      3. Characterizing Recombinant Proteins Using HPLC Gel Filtration and Mass Spectrometry
      4. Use of CID/ETD Mass Spectrometry to Analyze Glycopeptides
      5. De Novo Peptide Sequencing via Manual Interpretation of MS/MS Spectra
      6. Removal of Detergents from Proteins and Peptides in a Spin-Column Format
      7. Determining the Identity and Structure of Recombinant Proteins
      8. Enzymatic Approaches for Obtaining Amino Acid Sequence: On-Target Ladder Sequencing
      9. Glycoproteomics Using Chemical Immobilization
      10. Human Serum and Plasma Proteomics
      11. In-Gel Digestion of Proteins for MALDI-MS Fingerprint Mapping
      12. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface
      13. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using Microscale Capillary Liquid Chromatography
      14. Isolation of Organelles and Prefractionation of Protein Extracts Using Free-Flow Electrophoresis
      15. Large-Scale Identification of the Arginine Methylome by Mass Spectrometry
      16. Laser Capture Microdissection for Proteome Analysis
      17. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides
      18. Protein Profiling by Microscale Solution Isoelectrofocusing (MicroSol-IEF)
      19. Middle-Down and Top-Down Mass Spectrometric Analysis of Co-occurring Histone Modifications
      20. Organelle Proteomics
      21. Overview of Proteome Analysis
      22. Overview of Peptide and Protein Analysis by Mass Spectrometry
      23. Peptide Extraction from Formalin-Fixed Paraffin-Embedded Tissue
      24. Peptidomic Approaches to Study Proteolytic Activity
      25. Phosphoproteomics
      26. PRIDE: Data Submission and Analysis
      27. Determination of Protein Contacts by Chemical Cross-Linking With EDC and Mass Spectrometry
      28. Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching
      29. Sample Preparation and In-Solution Protease Digestion of Proteins for Chromatography-Based Proteomic Analysis
      30. Protein Profiling Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
      31. Identification of Protein-Protein Interactions by Surface Plasmon Resonance followed by Mass Spectrometry
      32. Preparation and Analysis of Proteins and Peptides Using MALDI TOF/TOF Mass Spectrometry
      33. Proteomic Analysis Using 2-D Liquid Separations of Intact Proteins From Whole-Cell Lysates
      34. Quantitative Protein Analysis Using Enzymatic [18O]Water Labeling
      35. Rapid Detergent Removal from Peptide Samples with Ethyl Acetate for Mass Spectrometry Analysis
      36. Sample Preparation for MALDI Mass Analysis of Peptides and Proteins
      37. Searching Sequence Databases Over the Internet: Protein Identification Using MS-Fit
      38. Searching Sequence Databases Over the Internet: Protein Identification Using MS-Tag
      39. Using Spectral Libraries for Peptide Identification from Tandem Mass Spectrometry (MS/MS) Data
      40. The Isotope-Coded Affinity Tag Method for Quantitative Protein Profile Comparison and Relative Quantitation of Cysteine Redox Modifications
      41. Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
    2. Proteomic Data Processing and Analysis
    3. Proteomic Methods
  18. RNA
  19. Safety
  20. Structural Analysis of Biomolecules
  21. Supporting Lab Techniques