Current Protocols in Pharmacology
Online ISBN: 9780471141754
DOI: 10.1002/0471141755
Archive
Current Protocols in Pharmacology Supplements
2012: Supplement 56
2011: Supplement 55 Supplement 54 Supplement 53 Supplement 52
2010: Supplement 51 Supplement 50 Supplement 49 Supplement 48
2009: Supplement 47 Supplement 46 Supplement 45 Supplement 44
2008: Supplement 43 Supplement 42 Supplement 41 Supplement 40
2007: Supplement 39 Supplement 38 Supplement 37 Supplement 36
2006: Supplement 35 Supplement 34 Supplement 33 Supplement 32
2005: Supplement 31 Supplement 30 Supplement 29 Supplement 28
2004: Supplement 27 Supplement 26 Supplement 25 Supplement 24
2003: Supplement 23 Supplement 22 Supplement 21 Supplement 20
2002: Supplement 19 Supplement 18 Supplement 17 Supplement 16
2001: Supplement 15 Supplement 14 Supplement 13 Supplement 12
2000: Supplement 11 Supplement 10 Supplement 9 Supplement 8
1999: Supplement 7 Supplement 6 Supplement 5 Supplement 4
1998: Supplement 3 Supplement 2 Supplement 1 Core publication
Supplement 56, March 2012
Unit 2.8 Evaluating Modulators of "Regulator of G-protein Signaling" (RGS) Proteins
Abstract | Full Text: HTML PDF
Unit 5.4 Models of Inflammation: Carrageenan- or Complete Freund's Adjuvant (CFA)–Induced Edema and Hypersensitivity in the Rat
Abstract | Full Text: HTML PDF
Unit 5.6 Models of Inflammation: Carrageenan Air Pouch
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Unit 10.5 Models of Neurological Disease (Substance Abuse): Self-Administration in Monkeys
Abstract | Full Text: HTML PDF
Unit 12.8 Measurement of Cell Death in Mammalian Cells
Abstract | Full Text: HTML PDF
Supplement 55, December 2011
Unit 4.8 In Vitro Opioid Receptor Assays
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Unit 7.3 Overview of Drug Product Development
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Unit 10.14 Measurement of Action Potential Generation in Isolated Canine Left Ventricular Midmyocardial Myocytes
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Unit 12.14 Measurement of Glucose Uptake in Cultured Cells
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Supplement 54, September 2011
Unit 10.13 The Fentanyl/Etomidate-Anesthetized Beagle (FEAB) Model in Safety Pharmacology Assessment
Abstract | Full Text: HTML PDF
Unit 13B.6 Preparation of HCV NS3 and NS5B Proteins to Support Small-Molecule Drug Discovery
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Unit 13B.7 Biochemical Evaluation of HCV NS3 Protease Inhibitors
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Unit 14.20 Orthotopic Models of Esophageal Carcinoma and Their Use in Drug Discovery
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Unit 14.21 Pre-Clinical Mouse Models of Primary and Metastatic Pleural Cancers of the Lung and Breast and the Use of Bioluminescent Imaging to Monitor Pleural Tumor Burden
Abstract | Full Text: HTML PDF
Supplement 53, June 2011
Unit 5.60 Animal Models of Systemic Lupus Erythematosus (SLE) and Ex Vivo Assay Design for Drug Discovery
Abstract | Full Text: HTML PDF
Unit 13B.5 In Vitro Anti-Hepatitis C Virus (HCV) Resistance Selection and Characterization
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Unit 14.19 Orthotopic Xenograft Model of Cervical Cancer for Studying Microenvironmental Effects on Metastasis Formation and Response to Drug Treatment
Abstract | Full Text: HTML PDF
Supplement 52, March 2011
Unit 1.22 Quantification of Allosteric Interactions at G Protein–Coupled Receptors Using Radioligand Binding Assays
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Unit 14.16 Establishment, Maintenance, and In Vitro and In Vivo Applications of Primary Human Glioblastoma Multiforme (GBM) Xenograft Models for Translational Biology Studies and Drug Discovery
Abstract | Full Text: HTML PDF
Unit 14.17 Preclinical Models of Pediatric Solid Tumors (Neuroblastoma) and Their Use in Drug Discovery
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Unit 14.18 Preclinical Orthotopic and Intracardiac Injection Models of Human Breast Cancer Metastasis to Bone and Their Use in Drug Discovery
Abstract | Full Text: HTML PDF
Supplement 51, December 2010
Unit 1.21 Overview of Receptor Allosterism
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Unit 10.12 Evaluation of Neurotoxicity Potential in Rats: The Functional Observational Battery
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Unit 13B.3 High-Throughput Screening of Viral Entry Inhibitors Using Pseudotyped Virus
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Unit 13B.4 Generation and Quantitation of Infectious Hepatitis C Virus Derived from Cell Culture (HCVcc)
Abstract | Full Text: HTML PDF
Unit 14.15 Preclinical Mouse Models of Human Prostate Cancer and Their Utility in Drug Discovery
Abstract | Full Text: HTML PDF
Supplement 50, September 2010
Unit 9.13 Label-Free Imaging and Temporal Signature in Phenotypic Cellular Assays: A New Approach to High-Content Screening
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Unit 9.14 Measuring Receptor Target Coverage: A Radioligand Competition Binding Protocol for Assessing the Association and Dissociation Rates of Unlabeled Compounds
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Unit 13A.8 Static Biofilm Cultures of Gram-Positive Pathogens Grown in a Microtiter Format Used for Anti-Biofilm Drug Discovery
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Unit 13B.1 Evaluation of Compound Activity Against Hepatitis C Virus in Replicon Systems
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Unit 13B.2 Studies on Hepatitis C Virus Resistance to Inhibitors in Replicon Systems
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Supplement 49, June 2010
Unit 3.12 Cyclic Nucleotide Phosphodiesterase Assay Technology
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Unit 5.8 Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice
Abstract | Full Text: HTML PDF
Unit 5.59 Novel Object Recognition in the Rat: A Facile Assay for Cognitive Function
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Unit 6.11 Pharmacological Applications of Baculovirus-Mediated Protein Expression in Mammalian Cells
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Unit 9.12 Overview on the Rule of Five
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Unit 14.14 Mouse Models of Human Bladder Cancer as a Tool for Drug Discovery
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Appendix 4 Guidelines for Animal Care and Use in Biomedical Research
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Supplement 48, March 2010
Unit 1.33 In Vitro Muscarinic Receptor Radioligand-Binding Assays
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Unit 4.10 Tachykinin Receptor Assays
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Unit 4.15 In Vitro Isolated Tissue Functional Muscarinic Receptor Assays
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Unit 12.13 In Vivo Pharmacodynamic Assays for M₂ and M₃ Muscarinic Receptors
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Supplement 47, December 2009
Unit 5.57 Overview of Mouse Models of Inflammatory Bowel Disease and Their Use in Drug Discovery
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Unit 5.58 Methods of Inducing Inflammatory Bowel Disease in Mice
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Unit 13A.7 Macromolecular Synthesis and Membrane Perturbation Assays for Mechanisms of Action Studies of Antimicrobial Agents
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Unit 14.13 Pre-Clinical Models of Renal Carcinoma and Their Utility in Drug Development
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Supplement 46, September 2009
Unit 4.19 5-Hydroxytryptamine Receptor Assays
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Unit 5.55 Chemically Induced Mouse Models of Colitis
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Unit 5.56 Monocrotaline-Induced Pulmonary Hypertension in Wistar Rats
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Unit 9.11 Natural Products as a Foundation for Drug Discovery
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Supplement 45, June 2009
Unit 4.7 P1 (Adenosine) Purinoceptor Assays (Updated)
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Unit 5.16 Models of Anxiety: Stress-Induced Hyperthermia (SIH) in Singly Housed Mice (Updated)
Abstract | Full Text: HTML PDF
Unit 5.22 Acute Seizure Tests in Epilepsy Research: Electroshock- and Chemical-Induced Convulsions in the Mouse (Updated)
Abstract | Full Text: HTML PDF
Unit 5.54 Models of Aspects of Schizophrenia: Behavioral Sensitization Induced by Subchronic Phencyclidine Administration (New)
Abstract | Full Text: HTML PDF
Unit 14.11 Genetically Engineered Mouse Models of Ovarian Cancer and Their Utility in Drug Discovery (New)
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Unit 14.12 Xenograft and Transgenic Mouse Models of Epithelial Ovarian Cancer and Non-Invasive Imaging Modalities to Monitor Ovarian Tumor Growth In Situ: Applications in Evaluating Novel Therapeutic Agents (New)
Abstract | Full Text: HTML PDF
Supplement 44, March 2009
Unit 5.1 Models of Cardiovascular Disease: Measurement of Arrhythmogenic/Antiarrhythmic Activity in the Guinea Pig
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Unit 5.53 Models of Cardiovascular Disease: Measurement of Antihypertensive Activity in the Conscious Rat (SHR, DOCA-Salt, and the Goldblatt Hypertension Models)
Abstract | Full Text: HTML PDF
Unit 9.4 Overview of High-Throughput Screening
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Supplement 43, December 2008
Unit 1.8 Radioligand Binding Characterization of Neuronal Nicotinic Acetylcholine Receptors
Abstract | Full Text: HTML PDF
Unit 5.18 Models of Anxiety: Ultrasonic Vocalizations of Isolated Rat Pups
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Unit 5.51 Animal Models of Collagen-Induced Arthritis
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Unit 5.52 A Model of Cystitis Pain in the Mouse
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Unit 12.12 In Vitro Assay of Angiogenesis: Inhibition of Capillary Tube Formation
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Unit 14.10 Murine Retroviral Bone Marrow Transplantation Models for the Study of Human Myeloproliferative Disorders
Abstract | Full Text: HTML PDF
Supplement 42, September 2008
Unit 4.1 Overview of Receptor Interactions of Agonists and Antagonists
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Unit 5.3 Gastrointestinal Models: Intestinal Transit, Gastric Emptying, and Ulcerogenic Activity in the Rat
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Unit 5.50 Cardiomyopathic Syrian Hamster as a Model of Congestive Heart Failure
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Unit 9.10 The IND Application
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Supplement 41, June 2008
Unit 1.2 Receptor Theory
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Unit 5.48 Fear-Potentiated Startle and Light-Enhanced Startle Models in Drug Discovery
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Unit 5.49 Rodent Model of Attention: The 5-Choice Serial Reaction Time Task
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Unit 7.12 The Ussing Chamber and Measurement of Drug Actions on Mucosal Ion Transport
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Unit 12.11 Drug Testing in Cellular Chemotaxis Assays
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Supplement 40, March 2008
Unit 5.45 Orthostatic Hypotension Induced by Postural Change in the Rat (Tilt Test)
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Unit 5.46 Mouse Models of Bleomycin-Induced Pulmonary Fibrosis
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Unit 5.47 Streptozotocin-Induced Diabetic Models in Mice and Rats
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Unit 14.9 Animal Models of Multiple Myeloma and Their Utility in Drug Discovery
Abstract | Full Text: HTML PDF
Supplement 39, December 2007
Unit 3.13 Assessing Sensitivity to Antibacterial Topoisomerase II Inhibitors
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Unit 7.11 Cytochrome P450 Inhibition Assays Using Traditional and Fluorescent Substrates
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Unit 11.10 Electrophysiology of Airway Nerves
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Unit 14.8 Preclinical Chemotherapeutic Tumor Models of Common Childhood Cancers: Solid Tumors, Acute Lymphoblastic Leukemia, and Disseminated Neuroblastoma
Abstract | Full Text: HTML PDF
Supplement 38, September 2007
Unit 5.8 Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice
Abstract | Full Text: HTML PDF
Unit 14.5 Metastatic Model of Colon Carcinoma in Mice: Utility in the Study of Tumor Growth and Progression
Abstract | Full Text: HTML PDF
Unit 14.6 Models of Melanoma Metastasis: Using a Transient siRNA-Based Protein Inhibition Strategy in Mice to Validate the Functional Relevance of Pharmacological Agents
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Unit 14.7 Application of Radiotherapy and Chemotherapy Protocols to Pre-Clinical Tumor Models
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Appendix 3A Assays for Determination of Protein Concentration
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Appendix 3C Purification and Concentration of DNA from Aqueous Solutions
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Supplement 37, June 2007
Unit 1.19 Characterization of Histaminergic Receptors
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Unit 14.1 Murine and Canine Models of Appendicular Osteosarcoma
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Unit 14.2 Carcinogen-Induced Animal Models of Head and Neck Squamous Cell Carcinoma
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Unit 14.3 Orthotopic Model of Human Pancreatic Ductal Adenocarcinoma and Cancer Cachexia in Nude Mice
Abstract | Full Text: HTML PDF
Unit 14.4 Orthotopic Models of Human Gastric Carcinoma in Nude Mice: Applications for Study of Tumor Growth and Progression
Abstract | Full Text: HTML PDF
Supplement 36, March 2007
Unit 1.5 Characterization of Adrenoceptors
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Unit 1.6 Characterization of Dopamine Receptors
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Unit 2.6 Agonist-Stimulated [ 35 S]GTPγS Binding
Abstract | Full Text: HTML PDF
Unit 6.10 Overview of Pharmacogenetics
Abstract | Full Text: HTML PDF
Supplement 35, December 2006
Unit 1.16 Benzodiazepine Binding to GABA A Receptors
Abstract | Full Text: HTML PDF
Unit 5.44 Rat Model for Evaluation of Therapeutics on Peripheral Vascular Resistance
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Unit 9.8 Approaches to the Molecular Modeling of 7-Transmembrane Helical Receptors
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Unit 9.9 Overview of Drug Discovery and Development
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Unit 12.3 Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
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Unit 12.10 Detection of Nitros(yl)ated Metabolites of Nitric Oxide (NO) In Vivo by Gas-Phase Chemiluminescence Assay
Abstract | Full Text: HTML PDF
Supplement 34, September 2006
Unit 5.43 Dog EEG for Wake-Promotion Studies
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Unit 7.5 Determination of Compound Binding to Plasma Proteins
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Unit 7.10 In Vitro Species Comparisons and Metabolite Identification
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Unit 13A.5 In Vivo Animal Models: Quantitative Models Used for Identifying Antibacterial Agents
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Unit 13A.6 In Vitro Antibacterial Resistance Selection and Quantitation
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Supplement 33, June 2006
Unit 1.3 Practical Aspects of Radioligand Binding
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Unit 10.7 An Overview of QT Interval Assessment in Safety Pharmacology
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Unit 10.11 Head-Out Plethysmography in Safety Pharmacology Assessment
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Unit 11.3 The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs
Abstract | Full Text: HTML PDF
Supplement 32, March 2006
Unit 1.1 Receptors as Drug Targets
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Unit 9.5 Testing for Inverse Agonism with Constitutive Receptor Systems
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Unit 10.1 Overview of Safety Pharmacology
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Unit 10.3 Toxicology in the Drug Discovery and Development Process
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Supplement 31, December 2005
Unit 2.1 Overview of Signal Transduction
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Unit 13A.1 Overview of Anti-Infective Drug Development
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Unit 13A.2 Overview of Antibacterial Target Selection
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Unit 13A.3 Basic Microbiological Techniques Used in Antibacterial Drug Discovery
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Unit 13A.4 Primary Rodent Infection Models for Testing Antibacterial Compound Efficacy In Vivo
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Supplement 30, September 2005
Unit 1.32 A Homogeneous Assay to Assess GABA Transporter Activity
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Unit 2.2 Adenylate and Guanylate Cyclase Assays
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Unit 2.7 Assay of Receptor-Stimulated Phosphoinositide Turnover
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Unit 7.1 Overview of Pharmacokinetics
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Supplement 29, June 2005
Unit 1.4 Characterization of Opioid and ORL1 Receptors
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Unit 5.41 Animal Models of Erectile Dysfunction
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Unit 5.42 MPTP-Induced Models of Parkinson's Disease in Mice and Non-Human Primates
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Unit 12.9 Functional Screening in the Melanophore Bioassay
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Supplement 28, March 2005
Unit 3.12 Assays for Cyclic Nucleotide-Specific Phosphodiesterases (Families 1 to 5)
Full Text: HTML PDF
Unit 5.39 Measurement and Characterization of Energy Expenditure as a Tool in the Development of Drugs for Metabolic Diseases, such as Obesity and Diabetes
Abstract | Full Text: HTML PDF
Unit 5.40 Measurement and Characterization of Energy Intake in the Mouse
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Supplement 27, December 2004
Unit 1.31 Overview of Approaches to the Identification of Inhibitors of Cytokine Action
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Unit 5.38 Animal Tests of Anxiety
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Unit 9.7 Overview on the Use of Therapeutic Antibodies in Drug Discovery
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Unit 10.10 Primary Observation (Irwin) Test in Rodents for Assessing Acute Toxicity of a Test Agent and its Effects on Behavior and Physiological Function
Abstract | Full Text: HTML PDF
Supplement 26, September 2004
Unit 5.37 Model of Attention Deficit Hyperactivity Disorder: Five-Trial, Repeated Acquisition Inhibitory Avoidance in Spontaneously Hypertensive Rat Pups
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Unit 7.9 Hepatic Clearance and Drug Metabolism Using Isolated Perfused Rat Liver
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Unit 11.9 Electrophysiological Characterization of Recombinant and Native P2X Receptors
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Supplement 25, June 2004
Unit 1.30 Characterization of CGRP Receptor Binding
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Unit 5.35 Models of Muscle Pain: Carrageenan Model and Acidic Saline Model
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Unit 5.36 Models of Visceral Pain: Colorectal Distension (CRD)
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Unit 12.8 Measurement of Cell Death in Mammalian Cells
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Supplement 24, March 2004
Unit 1.29 Characterization of Neurotensin Receptors
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Unit 5.34 A Rat Model of Postoperative Pain
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Unit 12.6 Methods of Measuring Internalization of G Protein–Coupled Receptors
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Unit 12.7 Measurement of VLA-4/CS-1 and VLA-4/VCAM Adhesion Inhibition
Abstract | Full Text: HTML PDF
Supplement 23, December 2003
Unit 7.7 Uptake Studies for Evaluating Activity of Efflux Transporters in a Cell Line Representative of the Blood-Brain Barrier
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Unit 7.8 In Vitro Drug Metabolism Using Liver Microsomes
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Unit 11.7 Overview of Electrophysiological Characterization of Neuronal Nicotinic Acetylcholine Receptors
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Unit 11.8 Electrophysiological Analysis of Tetrodotoxin-Resistant Sodium Channel Pharmacology
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Supplement 22, September 2003
Unit 1.28 Characterization of Melanocortin Receptors
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Unit 5.33 Electrical Amygdala Kindling
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Unit 9.4 Overview on High-Throughput Screening
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Unit 10.9 Respiratory Function Assessment in Safety Pharmacology
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Unit 12.5 Production and Use of HIV-1 Luciferase Reporter Viruses
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Supplement 21, June 2003
Unit 5.32 Models of Neuropathic Pain in the Rat
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Unit 6.10 Overview of Pharmacogenetics
Full Text: HTML PDF
Unit 9.6 Overview of Microarrays in Drug Discovery and Development
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Unit 11.6 Electrophysiological Analysis of ATP-Sensitive Potassium Channels in Mammalian Cells and Xenopus Oocytes
Abstract | Full Text: HTML PDF
Supplement 20, March 2003
Unit 5.30 Social Recognition Task in the Rat
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Unit 5.31 Emetic Liability Testing in Ferrets
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Unit 10.8 Patch-Clamp Studies of Human Cardiac Ion Channels in the Evaluation of Cardiac Electrophysiological Effects of Compounds
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Unit 11.5 Electrophysiological Analysis of Heterologously Expressed Kv and SK/IK Potassium Channels
Abstract | Full Text: HTML PDF
Supplement 19, December 2002
Unit 3.11 In Vitro Enzymatic Assays for Ser/Thr-Selective Protein Kinases
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Unit 7.6 Determination of the Stability of Drugs in Plasma
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Unit 10.4 Place Preference Test in Rodents
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Supplement 18, September 2002
Unit 4.19 5-Hydroxytryptamine Receptor Assays
Full Text: HTML PDF
Unit 5.28 Intraperitoneal and Subcutaneous Tumor Models for Assessing Anti-Neoplastic Agents in Rodents
Abstract | Full Text: HTML PDF
Unit 5.29 Electrocardiographic Toxicity in the Guinea Pig
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Unit 6.9 Tissue Expression Profiling Using Real-Time PCR
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Unit 7.5 Determination of Compound Binding to Plasma Proteins
Full Text: HTML PDF
Supplement 17, June 2002
Unit 1.27 Radioligand Binding Assays for the Glycine Site on N -Methyl-D-Aspartate Receptors
Abstract | Full Text: HTML PDF
Unit 7.4 In Vivo Measurement of Blood-Brain Barrier Permeability
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Unit 11.4 Use of Electrophysiological Methods in the Study of Recombinant and Native Neuronal Ligand-Gated Ion Channels
Abstract | Full Text: HTML PDF
Supplement 16, March 2002
Unit 1.26 Characterization of Cannabinoid Receptors
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Unit 5.25 Overview of Animal Models of Asthma
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Unit 5.26 Guinea Pig Models of Asthma
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Unit 5.27 Models of Cardiac Ischemia-Reperfusion Injury in Dogs and Rats
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Supplement 15, December 2001
Unit 3.9 Cytochrome P450 Assays
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Unit 3.10 Measurement of Glutamate Carboxypeptidase II (NAALADase) Enzyme Activity by the Hydrolysis of [ 3 H]- N -Acetylaspartylglutamate (NAAG)
Abstract | Full Text: HTML PDF
Unit 5.24 Classic In Vivo Cancer Models: Three Examples of Mouse Models Used in Experimental Therapeutics
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Unit 7.3 Overview of Drug Product Development
Abstract | Full Text: HTML PDF
Unit 11.3 The Action Potential of the Purkinje Fiber: An In Vitro Model for Evaluation of the Proarrhythmic Potential of Cardiac and Noncardiac Drugs
Full Text: HTML PDF
Supplement 14, September 2001
Unit 1.24 Characterization of Chemokine Receptors
Abstract | Full Text: HTML PDF
Unit 1.25 Characterization of Calcium Channel Binding
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Unit 4.17 Histamine Receptor Assays
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Unit 4.18 Prostanoid Receptor Assays
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Unit 12.4 Cellular Assays of Chemokine Receptor Activation
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Supplement 13, June 2001
Unit 3.7 Characterization of Matrix Metalloproteinase Inhibitors: Enzymatic Assays
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Unit 3.8 In Vitro Enzymatic Assays of Protein Tyrosine Phosphatase 1B
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Unit 5.5 Models of Inflammation: Adjuvant-Induced Arthritis in the Rat
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Unit 5.23 Characterization of Matrix Metalloproteinase Inhibitors: Angiogenesis and Tumor Models
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Unit 6.7 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
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Unit 6.8 Mobility Shift DNA-Binding Assay Using Gel Electrophoresis
Abstract | Full Text: HTML PDF
Supplement 12, April 2001
Unit 1.23 Characterization of 5-HT 1A,B and 5-HT 2A,C Serotonin Receptor Binding
Abstract | Full Text: HTML PDF
Unit 9.5 Screening in Constitutively Active Receptor Systems
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Unit 10.7 An Overview of QT Interval Assessment in Safety Pharmacology
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Unit 12.2 Chondrocyte Culture and Assay
Abstract | Full Text: HTML PDF
Unit 12.3 Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
Supplement 11, December 2000
Unit 1.20 [ 3 H]( )MK801 Radioligand Binding Assay at the N -Methyl-D-Aspartate Receptor
Abstract | Full Text: HTML PDF
Unit 1.21 Overview of Receptor Allosterism
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Unit 1.22 Quantification of Allosteric Interactions at G Protein Coupled Receptors Using Radioligand Binding Assays
Abstract | Full Text: HTML PDF
Unit 10.6 Vigilance-Controlled Quantified EEG in Safety Pharmacology
Abstract | Full Text: HTML PDF
Supplement 10, September 2000
Unit 5.8 Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice
Full Text: HTML PDF
Unit 5.22 Models of Epilepsy: Electroshock and Chemical Induced Convulsions in the Mouse
Full Text: HTML PDF
Unit 9.3 Overview of Combinatorial Chemistry
Abstract | Full Text: HTML PDF
Unit 9.4 Overview on High-Throughput Screening
Supplement 9, June 2000
Unit 1.19 Characterization of Histaminergic Receptors
Full Text: HTML PDF
Unit 3.6 Monoamine Oxidase Assays
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Unit 4.16 Cysteinyl Leukotriene Receptor Assays
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Unit 9.2 Cell-Based Assays Using the Fluorometric Imaging Plate Reader (FLIPR)
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Supplement 8, March 2000
Unit 1.4 Characterization of Opioid Receptors
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Unit 1.18 Characterization of the Picrotoxin Site of GABA A Receptors
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Unit 7.2 Estimating Intestinal Mucosal Permeation of Compounds Using Caco-2 Cell Monolayers
Abstract | Full Text: HTML PDF
Appendix 3F In Situ Hybridization to Cellular RNA
Abstract | Full Text: HTML PDF
Supplement 7, December 1999
Unit 2.7 Assay of Receptor-Stimulated Phosphoinositide Turnover
Full Text: HTML PDF
Unit 4.15 Muscarinic Receptor Assays
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Unit 5.20 Overview of the Use of Transgenic Animals in CNS Drug Discovery
Abstract | Full Text: HTML PDF
Unit 5.21 Models of Renal Insufficiency: The Anti-Thy-1.1 Model of Acute Proliferative Glomerulonephritis
Abstract | Full Text: HTML PDF
Unit 11.2 Electrophysiological Analysis of G Protein–Coupled Receptors in Mammalian Neurons
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Appendix 3D Fixation, Embedding, and Sectioning of Tissues, Embryos, and Single Cells
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Appendix 3E Cryosectioning
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Supplement 6, September 1999
Unit 1.17 Characterization of Potassium Channel Binding
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Unit 4.14 Dopamine 2 (DA-2) Receptor Assays Using Sympathetic Nerve Terminals
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Appendix 1B Receptor Nomenclature Guidelines
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Supplement 5, July 1999
Unit 1.16 Benzodiazepine Binding to GABA A Receptors
Unit 3.5 Protein Tyrosine Kinase Activity Assays
Abstract | Full Text: HTML PDF
Unit 5.18 Models of Anxiety: Ultrasonic Vocalizations of Isolated Rat Pups
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Unit 5.19 Models for Environmentally Induced Eating Disorders: Dietary Hyperphagia and Anorexia Nervosa
Abstract | Full Text: HTML PDF
Unit 6.4 Isotopic Assays for Reporter Gene Activity
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Unit 6.5 Nonisotopic Assays for Reporter Gene Activity
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Unit 6.6 Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics In Vivo
Abstract | Full Text: HTML PDF
Unit 6.7 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Supplement 4, March 1999
Unit 1.14 Characterization of Wild-Type Excitatory Amino Acid Ion Channel Receptors
Abstract | Full Text: HTML PDF
Unit 1.15 Characterization of Tachykinin Receptors
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Unit 4.12 Nicotinic Acetylcholine Receptor Assays
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Unit 4.13 Cholecystokinin (CCK) Assays
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Unit 5.17 Models for Assessing Antipsychotics: Antagonism of Amphetamine-Induced Hyperactivity and Stereotypies in Mice
Abstract | Full Text: HTML PDF
Unit 12.1 Overview of Cell and Tissue Culture Techniques
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Supplement 3, December 1998
Unit 5.14 Models of Neurological Disease (Alzheimer's Disease): Spatial Discrimination Water Maze Test in Septal-Lesioned Rats
Abstract | Full Text: HTML PDF
Unit 5.15 Models of Schizophrenia: Phencyclidine Disruption of Prepulse Inhibition (PPI) of Startle in Rats
Abstract | Full Text: HTML PDF
Unit 5.16 Models of Anxiety: Stress-Induced Hyperthermia (SIH) in Group-Housed Mice
Full Text: HTML PDF
Unit 8.2 Autoradiographic Localization of Growth Factor Receptors in Neuronal Tissues
Abstract | Full Text: HTML PDF
Unit 10.4 Place Preference Test in Rodents
Unit 10.5 Models of Neurological Disease (Substance Abuse): Self-Administration in Monkeys
Abstract | Full Text: HTML PDF
Unit 11.1 Overview of Electrophysiology
Abstract | Full Text: HTML PDF
Supplement 2, September 1998
Unit 1.8 Characterization of Neuronal Nicotinic Acetylcholine Receptors
Full Text: HTML PDF
Unit 1.13 Characterization of Corticotropin-Releasing Factor (CRF) Receptors
Abstract | Full Text: HTML PDF
Unit 2.6 Agonist-Stimulated [ 35 S]GTPγS Binding
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Unit 5.11 Models of Urogenital Dysfunction: Urinary Urge Incontinence (UUI) in Anesthetized Dog and Rat Models
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Unit 5.12 Models of Urogenital Dysfunction: Assessment of Volume-Evoked Micturition Reflex (VEMR) in the Conscious Rat by Cystometry
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Unit 5.13 Models of Dementia: Delayed Alternation in Aged Rats
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Unit 10.3 Toxicology in the Drug Discovery and Development Process
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Appendix 2B Standard Laboratory Equipment and Supplies
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Supplement 1, June 1998
Unit 1.10 Characterization of Angiotensin II Receptors
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Unit 1.11 Characterization of Neuropeptide Y (NPY) Receptors
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Unit 1.12 Characterization of Cholecystokinin (CCK) Receptors
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Unit 4.10 Tachykinin Receptor Assays
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Unit 4.11 Neuropeptide Y (NPY) Receptor Assays
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Unit 5.8 Models of Affective Illness: Behavioral Despair Tests in Rodents
Unit 5.9 Models of Affective Illness: Chronic Mild Stress
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Unit 5.10 Models of Urogenital Dysfunction: Benign Prostatic Hyperplasia (BPH)
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Core Publication, March 1998
Unit 1.1 Receptors as Drug Targets
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Unit 1.2 Receptor Theory
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Unit 1.3 Practical Aspects of Radioligand Binding
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Unit 1.4 Characterization of Opioid Receptors
Unit 1.5 Characterization of Adrenoceptors
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Unit 1.6 Characterization of Dopamine Receptors
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Unit 1.7 Characterization of GABA Receptors
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Unit 1.9 Characterization of P1 (Adenosine) Purinoceptors
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Unit 2.1 Overview of Signal Transduction
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Unit 2.2 Adenylyl and Guanylyl Cyclase Assays
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Unit 2.3 Overview of Phosphoinositide Hydrolysis
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Unit 2.4 Nitric Oxide Synthase Assays
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Unit 2.5 Analysis of Early Gene Responses
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Unit 3.1 Cyclooxygenase Assays
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Unit 3.2 HIV Protease Assays
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Unit 3.3 Topoisomerase Assays
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Unit 3.4 Protein Farnesyltransferase Assays
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Unit 4.1 Overview of Receptor Interactions of Agonists and Antagonists
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Unit 4.2 Overview of Length-Tension Relationships in Isolated Tissue
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Unit 4.3 Isolated Cardiac Muscle Assays
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Unit 4.4 Isolated Blood Vessel Assays
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Unit 4.5 α-Adrenoceptor Assays
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Unit 4.6 β-Adrenoceptor Assays
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Unit 4.7 P1 (Adenosine) Purinoceptor Assays
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Unit 4.8 Opioid Receptor Assays
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Unit 4.9 Angiotensin II Receptor Assays
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Unit 5.1 Models of Cardiovascular Disease: Measurement of Arrhythmogenic/Antiarrhythmic Activity in the Guinea Pig and Antihypertensive Activity in the Conscious Rat
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Unit 5.2 Models of Pulmonary Disease: Acute and Chronic Allergic Asthma in the Monkey and Acute and Chronic Viral Pulmonitis in the Mouse
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Unit 5.3 Gastrointestinal Models: Intestinal Transit and Ulcerogenic Activity in the Rat
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Unit 5.4 Models of Inflammation: Carrageenan-Induced Paw Edema in the Rat
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Unit 5.5 Models of Inflammation: Adjuvant-Induced Arthritis in the Rat
Unit 5.6 Models of Inflammation: Carrageenan Air Pouch in the Rat
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Unit 5.7 Models of Pain: Hot-Plate and Formalin Test in Rodents
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Unit 6.1 Overview of Receptor Cloning
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Unit 6.2 Receptor Reporter Systems
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Unit 6.3 Expression of Cloned Receptors in Mammalian Cell Lines
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Unit 7.1 Overview of Pharmacokinetics
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Unit 8.1 In Vitro Autoradiography
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Unit 9.1 Overview of Chemical Diversity
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Unit 10.1 Overview of Safety Pharmacology
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Unit 10.2 Models of Inflammation: Measuring Gastrointestinal Ulceration in the Rat
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Appendix 1A Enzyme Nomenclature
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Appendix 2A Common Stock Solutions, Buffers, and Media
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Appendix 3A Assays for Total Protein
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Appendix 3B Protein Analysis by SDS-PAGE and Detection by Coomassie Blue or Silver Staining
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Appendix 3C Purification and Concentration of DNA from Aqueous Solutions
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