Current Protocols in Neuroscience

Current Protocols in Neuroscience

Online ISBN: 9780471142300

DOI: 10.1002/0471142301

Archive


 

Current Protocols in Neuroscience Supplements

2014:    Supplement 69     Supplement 68     Supplement 67     Supplement 66    
2013:    Supplement 65     Supplement 64     Supplement 63     Supplement 62    
2012:    Supplement 61     Supplement 60     Supplement 59     Supplement 58    
2011:    Supplement 57     Supplement 56     Supplement 55     Supplement 54    
2010:    Supplement 53     Supplement 52     Supplement 51     Supplement 50     
2009:    Supplement 49     Supplement 48     Supplement 47     Supplement 46    
2008:    Supplement 45     Supplement 44     Supplement 43     Supplement 42
2007:    Supplement 41     Supplement 40     Supplement 39     Supplement 38    
2006:    Supplement 37     Supplement 36     Supplement 35     Supplement 34    
2005:    Supplement 33     Supplement 32     Supplement 31     Supplement 30    
2004:    Supplement 29     Supplement 28     Supplement 27     Supplement 26    
2003:    Supplement 25     Supplement 24     Supplement 23     Supplement 22    
2002:    Supplement 21     Supplement 20     Supplement 19     Supplement 18    
2001:    Supplement 17     Supplement 16     Supplement 15     Supplement 14    
2000:    Supplement 13     Supplement 12     Supplement 11     Supplement 10      
1999:    Supplement 9       Supplement 8       Supplement 7       Supplement 6      
1998:    Supplement 5       Supplement 4       Supplement 3       Supplement 2      
1997:    Supplement 1       Core publication




Supplement 69, October 2014

Unit 1.21 How to Prepare Neuroanatomical Image Data
         Abstract | Full Text: HTML PDF

Unit 3.27 Primary Porcine Brain Microvessel Endothelial Cell isolation and Culture
         Abstract | Full Text: HTML PDF

Unit 8.31 Examining Object Location and Object Recognition Memory in Mice
         Abstract | Full Text: HTML PDF

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Supplement 68, July 2014

Unit 1.5 Transneuronal Circuit Analysis with Pseudorabies Viruses
         Abstract | Full Text: HTML PDF

Unit 9.49 "Drinking in the Dark" (DID): A Simple Mouse Model of Binge-Like Alcohol Intake
         Abstract | Full Text: HTML PDF

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Supplement 67, April 2014

Unit 2.8 3-D Reconstruction of Neurons from Multichannel Confocal Laser Scanning Image Series
         Abstract | Full Text: HTML PDF

Unit 9.47 Rodent Model of Activity-Based Anorexia
         Abstract | Full Text: HTML PDF

Unit 9.48 The 3-NP Model of Striatal Neurodegeneration
         Abstract | Full Text: HTML PDF

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Supplement 66, January 2014

Unit 2.21 Imaging mGluR5 Dynamics in Astrocytes Using Quantum Dots
         Abstract | Full Text: HTML PDF

Unit 2.22 Optical Clearing of Fixed Brain Samples Using SeeDB
         Abstract | Full Text: HTML PDF

Unit 8.30 Assessing Delay Discounting in Mice
         Abstract | Full Text: HTML PDF

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Supplement 65, October 2013

Unit 2.20 Single-Molecule Tracking Photoactivated Localization Microscopy to Map Nano-Scale Structure and Dynamics in Living Spines
         Abstract | Full Text: HTML PDF

Unit 4.35 Manipulating Gene Expression in Projection-Specific Neuronal Populations Using Combinatorial Viral Approaches
         Abstract | Full Text: HTML PDF

Unit 9.46 Modeling an Inflammation-Related Depressive Phenotype in Mice Using Bacille Calmette-Guérin
         Abstract | Full Text: HTML PDF

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Supplement 64, July 2013

Unit 2.19 Assembly of Synapses: Biomimetic Assays to Control Neurexin/Neuroligin Interactions at the Neuronal Surface
         Abstract | Full Text: HTML PDF

Unit 6.27 Incubation of Fear
         Abstract | Full Text: HTML PDF

Unit 9.44 A Choice-Based Screening Method for Compulsive Drug Users in Rats
         Abstract | Full Text: HTML PDF

Unit 9.45 Animal Models of Post-Traumatic Stress Disorder
         Abstract | Full Text: HTML PDF

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Supplement 63, April 2013

Unit 5.22 Quantifying Cellular Dynamics by Fluorescence Resonance Energy Transfer (FRET) Microscopy
         Abstract | Full Text: HTML PDF

Unit 7.4 Detection and Quantification of Neurotransmitters in Dialysates
         Abstract | Full Text: HTML PDF

Unit 9.42 Social Fear Conditioning as an Animal Model of Social Anxiety Disorder
         Abstract | Full Text: HTML PDF

Unit 9.43 The MAM Rodent Model of Schizophrenia
         Abstract | Full Text: HTML PDF

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Supplement 62, January 2013

Unit 2.18 Tracking Quantum Dot–Tagged Calcium Channels at Vertebrate Photoreceptor Synapses: Retinal Slices and Dissociated Cells
         Abstract | Full Text: HTML PDF

Unit 4.13 Generation of High-Titer Defective HSV-1 Vectors
         Abstract | Full Text: HTML PDF

Unit 6.26 Electrophysiology Tool Construction
         Abstract | Full Text: HTML PDF

Unit 9.41 Blast Traumatic Brain Injury in the Rat Using a Blast Overpressure Model
         Abstract | Full Text: HTML PDF

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Supplement 61, October 2012

Unit 2.17 Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F-actin
         Abstract | Full Text: HTML PDF

Unit 4.12 Overview of Gene Delivery into Cells Using HSV-1-Based Vectors
         Abstract | Full Text: HTML PDF

Unit 9.39 Prime-, Stress-, and Cue-Induced Reinstatement of Extinguished Drug-Reinforced Responding in Rats: Cocaine as the Prototypical Drug of Abuse
         Abstract | Full Text: HTML PDF

Unit 9.40 Rodent Models of Colorectal Distension
         Abstract | Full Text: HTML PDF

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Supplement 60, July 2012

Unit 3.26 Culturing of Cerebellar Granule Cells to Study Neuronal Migration: Gradient and Local Perfusion Assays
         Abstract | Full Text: HTML PDF

Unit 4.14 Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
         Abstract | Full Text: HTML PDF

Unit 7.23 High-Affinity Choline Uptake (HACU) and Choline Acetyltransferase (ChAT) Activity in Neuronal Cultures for Mechanistic and Drug Discovery Studies
         Abstract | Full Text: HTML PDF

Unit 7.24 Direct Bisulfite Sequencing for Examination of DNA Methylation with Gene and Nucleotide Resolution from Brain Tissues
         Abstract | Full Text: HTML PDF

Unit 8.29 Methods for Studying Habitual Behavior in Mice
         Abstract | Full Text: HTML PDF

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Supplement 59, April 2012

Unit 1.20 Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice
         Abstract | Full Text: HTML PDF

Unit 2.7 Characterizing Synaptic Vesicle Proteins Using Synaptosomal Fractions and Cultured Hippocampal Neurons
         Abstract | Full Text: HTML PDF

Unit 3.25 Culturing Embryonic Nasal Explants for Developmental and Physiological Study
         Abstract | Full Text: HTML PDF

Unit 5.30 A Protein Cross-Linking Assay for Measuring Cell Surface Expression of Glutamate Receptor Subunits in the Rodent Brain After In Vivo Treatments
         Abstract | Full Text: HTML PDF

Unit 9.38 Diet-Induced Models of Obesity (DIO) in Rodents
         Abstract | Full Text: HTML PDF

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Supplement 58, January 2012

Unit 2.15 Confocal Laser Scanning Microscopic Photoconversion: A New Method to Stabilize Fluorescently Labeled Cellular Elements for Electron Microscope Analysis
         Abstract | Full Text: HTML PDF

Unit 2.16 Use of Channelrhodopsin for Activation of CNS Neurons
         Abstract | Full Text: HTML PDF

Unit 3.24 Culture of Mouse Olfactory Sensory Neurons
         Abstract | Full Text: HTML PDF

Unit 8.28 Analysis of Skilled Forelimb Movement in Rats: The Single Pellet Reaching Test and Staircase Test
         Abstract | Full Text: HTML PDF

Unit 9.37 Pentylenetetrazol (PTZ) Kindling Model of Epilepsy
         Abstract | Full Text: HTML PDF

Unit 10.5 A Rubric for Extracting Idea Density from Oral Language Samples
         Abstract | Full Text: HTML PDF

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Supplement 57, October 2011

Unit 2.14 High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy
         Abstract | Full Text: HTML PDF

Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration
         Abstract | Full Text: HTML PDF

Unit 4.22 Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
         Abstract | Full Text: HTML PDF

Unit 8.27 A Mouse Model for Observational Fear Learning and the Empathetic Response
         Abstract | Full Text: HTML PDF

Appendix 1E Transfection by Electroporation
         Abstract | Full Text: HTML PDF

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Supplement 56, July 2011

Unit 2.2 Basic Confocal Microscopy
         Abstract | Full Text: HTML PDF

Unit 3.23 In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
         Abstract | Full Text: HTML PDF

Unit 8.26 Automated Three-Chambered Social Approach Task for Mice
         Abstract | Full Text: HTML PDF

Appendix 1K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
         Abstract | Full Text: HTML PDF

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Supplement 55, April 2011

Unit 2.13 DiOlistic Labeling in Fixed Brain Slices: Phenotype, Morphology, and Dendritic Spines
         Abstract | Full Text: HTML PDF

Unit 4.4 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
         Abstract | Full Text: HTML PDF

Unit 4.30 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
         Abstract | Full Text: HTML PDF

Unit 8.10A Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice
         Abstract | Full Text: HTML PDF

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Supplement 54, January 2011

Unit 6.13 Synaptic Plasticity in the Hippocampal Slice Preparation
         Abstract | Full Text: HTML PDF

Unit 9.35 Overview of Animal Models of Attention Deficit Hyperactivity Disorder (ADHD)
         Abstract | Full Text: HTML PDF

Unit 9.36 Open-Space Forced Swim Model of Depression for Mice
         Abstract | Full Text: HTML PDF

Unit 10.4 Clinical and Neuroimaging Assessments for Research Studies (Including Drug Trials) in Multiple Sclerosis
         Abstract | Full Text: HTML PDF

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Supplement 53, October 2010

Unit 3.22 A Method for Preparing Primary Retinal Cell Cultures for Evaluating the Neuroprotective and Neuritogenic Effect of Factors on Axotomized Mature CNS Neurons
         Abstract | Full Text: HTML PDF

Unit 4.21 Production and Titration of Lentiviral Vectors
         Abstract | Full Text: HTML PDF

Unit 8.5I Social Recognition Assay in the Rat
         Abstract | Full Text: HTML PDF

Unit 8.25 Modeling Appetitive Pavlovian-Instrumental Interactions in Mice
         Abstract | Full Text: HTML PDF

Unit 9.34 Drug-Induced Sexual Dysfunction in Rats
         Abstract | Full Text: HTML PDF

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Supplement 52, July 2010

Unit 6.25 Electroencephalography (EEG) and Event-Related Potentials (ERPs) with Human Participants
         Abstract | Full Text: HTML PDF

Unit 7.5 Analyzing Binding Data
         Abstract | Full Text: HTML PDF

Unit 7.22 Isolation of Mitochondria from the CNS
         Abstract | Full Text: HTML PDF

Unit 9.33 A Murine Model of Atypical Antipsychotic-Induced Weight Gain and Metabolic Dysregulation
         Abstract | Full Text: HTML PDF

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Supplement 51, April 2010

Unit 4.34 Transfection of Mouse Cochlear Explants by Electroporation
         Abstract | Full Text: HTML PDF

Unit 5.12 The Use of Phage Display in Neurobiology
         Abstract | Full Text: HTML PDF

Unit 5.29 Visualization of G Protein–Coupled Receptor (GPCR) Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC)
         Abstract | Full Text: HTML PDF

Unit 9.12 Operant Self-Administration Models for Testing the Neuropharmacological Basis of Ethanol Consumption in Rats
         Abstract | Full Text: HTML PDF

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Supplement 50, January 2010

Unit 1.19 Mapping Lineage Using BAC-Cre Reporter Lines
         Abstract | Full Text: HTML PDF

Unit 2.1 Fluorescence Microscopy: A Concise Guide to Current Imaging Methods
         Abstract | Full Text: HTML PDF

Unit 2.5 Leading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
         Abstract | Full Text: HTML PDF

Unit 4.24 Gene Transfer into Rat Brain Using Adenoviral Vectors
         Abstract | Full Text: HTML PDF

Unit 4.33 Generation of Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) Using Directed Molecular Evolution
         Abstract | Full Text: HTML PDF

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Supplement 49, October 2009

Unit 2.3 Imaging Nervous System Activity
         Abstract | Full Text: HTML PDF

Unit 6.24 EEG Recording in Rodents, with a Focus on Epilepsy
         Abstract | Full Text: HTML PDF

Unit 9.32 Recapitulation and Reversal of a Persistent Depression-like Syndrome in Rodents
         Abstract | Full Text: HTML PDF

Unit 10.1 Assessment of Parkinson Disease Manifestations
         Abstract | Full Text: HTML PDF

Unit 10.2 EEG Recording and Analysis for Sleep Research
         Abstract | Full Text: HTML PDF

Unit 10.3 Neuropsychological Assessment in the Diagnosis of Alzheimer's Disease
         Abstract | Full Text: HTML PDF

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Supplement 48, July 2009

Unit 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR
         Abstract | Full Text: HTML PDF

Unit 5.5 Production of Polyclonal Antisera
         Abstract | Full Text: HTML PDF

Unit 7.4 Detection and Quantification of Neurotransmitters in Dialysates
         Abstract | Full Text: HTML PDF

Unit 7.17 Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
         Abstract | Full Text: HTML PDF

Unit 8.24 Simple Behavioral Assessment of Mouse Olfaction
         Abstract | Full Text: HTML PDF

Appendix 4I Assessing Reproductive Status/Stages in Mice
         Abstract | Full Text: HTML PDF

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Supplement 47, April 2009

Unit 3.6 Differentiation of Embryonic Stem Cells
         Abstract | Full Text: HTML PDF

Unit 4.32 Transfection of Cultured Primary Neurons via Nucleofection
         Abstract | Full Text: HTML PDF

Unit 7.1 Overview of Brain Microdialysis
         Abstract | Full Text: HTML PDF

Unit 7.2 Microdialysis in Rodents
         Abstract | Full Text: HTML PDF

Unit 8.23 Fear Extinction in Rodents
         Abstract | Full Text: HTML PDF

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Supplement 46, January 2009

Unit 1.18 Evolution of Neurotoxins: From Research Modalities to Clinical Realities
         Abstract | Full Text: HTML PDF

Unit 5.28 Identifying Novel Protein-Protein Interactions Using Co-Immunoprecipitation and Mass Spectroscopy
         Abstract | Full Text: HTML PDF

Unit 9.31 An Animal Model of Premenstrual Dysphoric Disorder Sensitive to Antidepressants
         Abstract | Full Text: HTML PDF

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Supplement 45, October 2008

Unit 2.12 The Importance of Titrating Antibodies for Immunocytochemical Methods
         Abstract | Full Text: HTML PDF

Unit 4.23 Gene Transfer into Neural Cells In Vitro Using Adenoviral Vectors
         Abstract | Full Text: HTML PDF

Unit 4.28 Gene Expression Analysis of Neural Cells and Tissues Using DNA Microarrays
         Abstract | Full Text: HTML PDF

Unit 9.30 Animal Models of Obsessive-Compulsive Disorder
         Abstract | Full Text: HTML PDF

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Supplement 44, July 2008

Unit 3.21 Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer
         Abstract | Full Text: HTML PDF

Unit 4.31 Conditional Gene Expression and Targeting in Neuroscience Research
         Abstract | Full Text: HTML PDF

Unit 7.18 Assessment of Cell Viability in Primary Neuronal Cultures
         Abstract | Full Text: HTML PDF

Unit 9.28 The Majchrowicz Binge Alcohol Protocol: An Intubation Technique to Study Alcohol Dependence in Rats
         Abstract | Full Text: HTML PDF

Unit 9.29 Vapor Inhalation of Alcohol in Rats
         Abstract | Full Text: HTML PDF

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Supplement 43, April 2008

Unit 1.17 Retrograde Axonal Tracing with Fluorescent Markers
         Abstract | Full Text: HTML PDF

Unit 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function
         Abstract | Full Text: HTML PDF

Unit 2.11 Ballistic Labeling with Fluorescent Dyes and Indicators
         Abstract | Full Text: HTML PDF

Unit 3.20 Dissection and Culture of Embryonic Spinal Commissural Neurons
         Abstract | Full Text: HTML PDF

Unit 9.27 Schedule-Induced Polydipsia: A Rat Model of Obsessive-Compulsive Disorder
         Abstract | Full Text: HTML PDF

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Supplement 42, January 2008

Unit 1.16 Biochemical Fractionation of Brain Tissue for Studies of Receptor Distribution and Trafficking
         Abstract | Full Text: HTML PDF (421K)

Unit 3.10 Neural Stem Cell Transplantation in Mouse Brain
         Abstract | Full Text: HTML PDF (599K)

Unit 9.26 Overview of Mouse Assays of Ethanol Intoxication
         Abstract | Full Text: HTML PDF (241K)

Appendix 4B Mouse and Rat Anesthesia and Analgesia
         Abstract | Full Text: HTML PDF (693K)

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Supplement 41, October 2007

Unit 4.22 Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
         Abstract | Full Text: HTML PDF (670K)

Unit 5.27 Split-Ubiquitin System for Identifying Protein-Protein Interactions in Membrane and Full-Length Proteins
         Abstract | Full Text: HTML PDF (1219K)

Unit 8.9 Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
         Abstract | Full Text: HTML PDF (322K)

Unit 9.25 Ratings of L-DOPA-Induced Dyskinesia in the Unilateral 6-OHDA Lesion Model of Parkinson's Disease in Rats and Mice
         Abstract | Full Text: HTML PDF (640K)

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  Supplement 40, July 2007

Unit 4.14 Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
         Abstract | Full Text:  HTML   PDF (414K)

Unit 4.29 Overview of Gene Targeting by Homologous Recombination
         Abstract | Full Text:  HTML   PDF (680K)

Unit 5.7 Production of Antisera Using Fusion Proteins
         Abstract | Full Text:  HTML   PDF (253K)

Unit 8.1 Locomotor Behavior
         Abstract | Full Text:  HTML   PDF (195K)

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  Supplement 39, April 2007

Unit 1.15 Histochemical Methods for the Detection of Apoptosis in the Nervous System
         Abstract | Full Text:  HTML   PDF (277K)

Unit 5.26 Lentiviral Strategies for RNAi Knockdown of Neuronal Genes
         Abstract | Full Text:  HTML   PDF (362K)

Unit 8.22 Delay Discounting and Impulsive Choice in the Rat
         Abstract | Full Text:  HTML   PDF (701K)

Unit 9.24 Overview of Animal Models of Schizophrenia
         Abstract | Full Text:  HTML   PDF (176K)

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  Supplement 38, January 2007

Unit 6.22 Whole-Cell Recording In Vivo
         Abstract | Full Text:  HTML   PDF (142K)

Unit 6.23 Electrophysiological Recordings from Neonatal Neocortical Brain Slices
         Abstract | Full Text:  HTML   PDF (181K)

Unit 7.21 Assays for Cyclic Nucleotide-Specific Phosphodiesterases (PDEs) in the Central Nervous System (PDE1, PDE2, PDE4, and PDE10)
         Abstract | Full Text:  HTML   PDF (271K)

Appendix 3B Techniques for Mammalian Cell Tissue Culture
         Abstract | Full Text:  HTML   PDF (228K)

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  Supplement 37, October 2006

Unit 1.14 Anterograde Axonal Tract Tracing
         Abstract | Full Text:  HTML   PDF (440K)

Unit 3.9 Quantitative Analysis of In Vivo Cell Proliferation
         Abstract | Full Text:  HTML   PDF (317K)

Unit 4.21 Production and Titration of Lentiviral Vectors
         Abstract | Full Text:  HTML   PDF (829K)

Unit 6.20 Caged Molecules: Principles and Practical Considerations
         Abstract | Full Text:  HTML   PDF (854K)

Unit 6.21 Photolysis of Caged Neurotransmitters: Theory and Procedures for Light Delivery
         Abstract | Full Text:  HTML   PDF (323K)

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  Supplement 36, July 2006

Unit 2.10 Whole-Mount Immunohistochemistry of the Brain
         Abstract | Full Text:  HTML   PDF (323K)

Unit 3.19 Rodent Sensory Neuron Culture and Analysis
         Abstract | Full Text:  HTML   PDF (244K)

Unit 9.23A Binge Eating in Rats Produced by Combining Dieting with Stress
         Abstract | Full Text:  HTML   PDF (226K)

Unit 9.23B Binge Eating in Rats with Limited Access to Vegetable Shortening
         Abstract | Full Text:  HTML   PDF (163K)

Unit 9.23C Sugar Bingeing in Rats
         Abstract | Full Text:  HTML   PDF (153K)

Unit 9.23D Characterization of Energy Expenditure in Rodents by Indirect Calorimetry
         Abstract | Full Text:  HTML   PDF (342K)

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  Supplement 35, April 2006

Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use for In Vitro and In Vivo Administration
         Abstract | Full Text:  HTML   PDF (591K)

Unit 5.24 Immunoprecipitation
         Abstract | Full Text:  HTML   PDF (525K)

Unit 5.25 Detection of Protein-Protein Interactions by Coprecipitation
         Abstract | Full Text:  HTML   PDF (261K)

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  Supplement 34, January 2006

Unit 5.22 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
         Abstract | Full Text:  HTML   PDF (335K)

Unit 5.23 Monitoring Protein-Protein Interactions in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
         Abstract | Full Text:  HTML   PDF (274K)

Unit 8.4 Application of Experimental Stressors in Laboratory Rodents
         Abstract | Full Text:  HTML   PDF (144K)

Unit 8.21A Overview of Methods for Assessing the Mouse Auditory System
         Abstract | Full Text:  HTML   PDF (39K)

Unit 8.21B Measurement of the Auditory Brainstem Response (ABR) to Study Auditory Sensitivity in Mice
         Abstract | Full Text:  HTML   PDF (116K)

Unit 8.21C Assessment of Cochlear Function in Mice: Distortion-Product Otoacoustic Emissions
         Abstract | Full Text:  HTML   PDF (550K)

Unit 8.21D Behavioral Assessment of Hearing in Mice—Conditioned Suppression
         Abstract | Full Text:  HTML   PDF (231K)

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  Supplement 33, October 2005

Unit 3.18 Fluorescence-Based Sorting of Neural Stem Cells and Progenitors
         Abstract | Full Text:  HTML   PDF (1025K)

Unit 8.20 Olfactometry with Mice
         Abstract | Full Text:  HTML   PDF (586K)

Unit 9.22 Overview on Rodent Models of Alzheimer's Disease
         Abstract | Full Text:  HTML   PDF (149K)

Appendix 4F Parenteral Injections
         Abstract | Full Text:  HTML   PDF (137K)

Appendix 4G Blood Collection
         Abstract | Full Text:  HTML   PDF (112K)

Appendix 4H Euthanasia
         Abstract | Full Text:  HTML   PDF (44K)

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  Supplement 32, July 2005

Unit 2.8 3-D Reconstruction of Neurons from Multichannel Confocal Laser Scanning Image Series
         Abstract | Full Text:  HTML   PDF (899K)

Unit 2.9 Two-Photon Imaging in Live Rodents
         Abstract | Full Text:  HTML   PDF (707K)

Unit 9.20 Chronic Intravenous Drug Self-Administration in Rats and Mice
         Abstract | Full Text:  HTML   PDF (1482K)

Unit 9.21 Intravenous Self-Administration Techniques in Monkeys
         Abstract | Full Text:  HTML   PDF (241K)

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  Supplement 31, April 2005

Unit 5.20 An Overview on the Generation of BAC Transgenic Mice for Neuroscience Research
         Abstract | Full Text:  HTML   PDF (158K)

Unit 5.21 Modification of Bacterial Artificial Chromosomes (BACs) and Preparation of Intact BAC DNA for Generation of Transgenic Mice
         Abstract | Full Text:  HTML   PDF (227K)

Unit 8.5H Assaying Aspects of Attention and Impulse Control in Mice Using the 5-Choice Serial Reaction Time Task
         Abstract | Full Text:  HTML   PDF (348K)

Unit 9.19 Chemoconvulsant Model of Chronic Spontaneous Seizures
         Abstract | Full Text:  HTML   PDF (127K)

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  Supplement 30, January 2005

Unit 7.15 Scintillation Proximity Assay
         Abstract | Full Text:  HTML   PDF (843K)

Unit 8.19 Defensive Responses to Predator Threat in the Rat and Mouse
         Abstract | Full Text:  HTML   PDF (352K)

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  Supplement 29, October 2004

Unit 5.19 Immunoblotting and Immunodetection
         Abstract | Full Text:  HTML   PDF (304K)

Unit 6.19 Patch-Clamp Recording from Neuronal Dendrites
         Abstract | Full Text:  HTML   PDF (287K)

Unit 8.6C Direct, Continuous Behavioral Analysis of Drug Action on Feeding
         Abstract | Full Text:  HTML   PDF (116K)

Unit 8.6E Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
         Abstract | Full Text:  HTML   PDF (120K)

Unit 9.18 Animal Models of Painful Diabetic Neuropathy: The STZ Rat Model
         Abstract | Full Text:  HTML   PDF (104K)

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  Supplement 28, July 2004

Unit 2.7 Multidisciplinary Approaches for Characterizing Synaptic Vesicle Proteins
         Abstract | Full Text:  HTML   PDF (249K)

Unit 8.5G Social Transmission of Food Preference in Mice
         Abstract | Full Text:  HTML   PDF (138K)

Unit 9.2 Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
         Abstract | Full Text:  HTML   PDF (338K)

Appendix 5B Databases and Web Sites for Neurogenetics
         Abstract | Full Text:  HTML   PDF (54K)

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  Supplement 27, April 2004

Unit 1.13 Quantified Assessment of Terminal Density and Innervation
         Abstract | Full Text:  HTML   PDF (355K)

Unit 4.16 Protein Expression in the Drosophila Schneider 2 Cell System
         Abstract | Full Text:  HTML   PDF (173K)

Unit 8.5C Cued and Contextual Fear Conditioning in Mice
         Abstract | Full Text:  HTML   PDF (509K)

Unit 8.6G Macronutrient Selection in Experimental Animals
         Abstract | Full Text:  HTML   PDF (246K)

Appendix 4D Handling and Restraint
         Abstract | Full Text:  HTML   PDF (248K)

Appendix 4E Animal Identification
         Abstract | Full Text:  HTML   PDF (94K)

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  Supplement 26, January 2004

Unit 1.12 Intracellular and Juxtacellular Staining with Biocytin
         Abstract | Full Text:  HTML   PDF (222K)

Unit 3.2 Long-Term Culture of Hippocampal Neurons
         Abstract | Full Text:  HTML   PDF (359K)

Unit 6.3 Fabrication of Patch Pipets
         Abstract | Full Text:  HTML   PDF (1035K)

Unit 8.3 Animal Tests of Anxiety
         Abstract | Full Text:  HTML   PDF (238K)

Unit 8.5A Assessment of Spatial Memory Using the Radial Arm Maze and Morris Water Maze
         Abstract | Full Text:  HTML   PDF (170K)

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  Supplement 25, October 2003

Unit 1.2 Immunohistochemical Localization of Proteins in the Nervous System
         Abstract | Full Text:  HTML   PDF (179K)

Unit 1.3 Hybridization Histochemistry of Neural Transcripts
         Abstract | Full Text:  HTML   PDF (249K)

Unit 6.18 Recording in the Cerebellar Slice
         Abstract | Full Text:  HTML   PDF (215K)

Unit 8.5F Assessment of Learning and Memory Using the Autoshaping of Operant Responding in Mice
         Abstract | Full Text:  HTML   PDF (249K)

Unit 8.18 Assessment of Developmental Milestones in Rodents
         Abstract | Full Text:  HTML   PDF (186K)

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  Supplement 24, July 2003

Unit 1.11 Counting Cells in Sectioned Material: A Suite of Techniques, Tools, and Tips
         Abstract | Full Text:  HTML   PDF (312K)

Unit 8.17 Assessment of Murine Startle Reactivity, Prepulse Inhibition, and Habituation
         Abstract | Full Text:  HTML   PDF (383K)

Unit 9.17 Measurement of Panic-Like Responses Following Intravenous Infusion of Sodium Lactate in Panic-Prone Rats
         Abstract | Full Text:  HTML   PDF (526K)

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  Supplement 23, April 2003

Unit 1.10 Quantitative In Situ Hybridization for the Study of Gene Expression at the Regional and Cellular Levels
         Abstract | Full Text:  HTML   PDF (289K)

Unit 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function
         Full Text:  HTML   PDF (211K)

Unit 5.1 RNA Analysis by Nuclease Protection
         Abstract | Full Text:  HTML   PDF (594K)

Unit 6.17 Imaging Nervous System Activity with Voltage-Sensitive Dyes
         Abstract | Full Text:  HTML   PDF (403K)

Unit 9.16 Inducing Photochemical Cortical Lesions in Rat Brain
         Abstract | Full Text:  HTML   PDF (368K)

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  Supplement 22, January 2003

Unit 8.5E Assessment of Sustained and Divided Attention in Rats
         Abstract | Full Text:  HTML   PDF (167K)

Unit 8.16 Mouse Social Recognition and Preference
         Abstract | Full Text:  HTML   PDF (451K)

Unit 9.14 Models of Neuropathic Pain in the Rat
         Abstract | Full Text:  HTML   PDF (225K)

Unit 9.15 Place Preference Test in Rodents
         Abstract | Full Text:  HTML   PDF (318K)

Appendix 5A Internet Resources for Genomic, Bioinformatics, and Medical Genetics Information
         Abstract | Full Text:  HTML   PDF (68K)

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  Supplement 21, October 2002

Unit 4.29 Overview of Gene Targeting by Homologous Recombination
         Full Text:  HTML   PDF (163K)

Unit 4.30 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
         Abstract | Full Text:  HTML   PDF (166K)

Unit 6.16 Chronic Recording of Extracellular Neuronal Activity in Behaving Animals
         Abstract | Full Text:  HTML   PDF (278K)

Unit 8.5D Social Learning of Food Preferences in Rodents: Rapid Appetitive Learning
         Abstract | Full Text:  HTML   PDF (124K)

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  Supplement 20, July 2002

Unit 2.5 Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
         Full Text:  HTML   PDF (198K)

Unit 4.28 Gene Expression Analysis Using c DNA Microarrays
         Full Text:  HTML   PDF (273K)

Unit 9.13 Models of Amyotrophic Lateral Sclerosis
         Abstract | Full Text:  HTML   PDF (418K)

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  Supplement 19, April 2002

Unit 4.27 cDNA Library Construction from Single Cells
         Abstract | Full Text:  HTML   PDF (230K)

Unit 6.15 Preparation and Maintenance of Organotypic Cultures for Multi-Electrode Array Recordings
         Abstract | Full Text:  HTML   PDF (194K)

Unit 8.11B Fear-Potentiated Startle in Mice
         Abstract | Full Text:  HTML   PDF (301K)

Unit 9.11 Intravenous Self-Administration of Ethanol in Mice
         Abstract | Full Text:  HTML   PDF (185K)

Unit 9.12 Preclinical Models to Evaluate Potential Pharmacotherapeutic Agents in Treating Alcoholism and Studying the Neuropharmacological Bases of Ethanol-Seeking Behaviors in Rats
         Full Text:  HTML   PDF (354K)

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  Supplement 18, February 2002

Unit 2.4 Electronic Imaging in Neuroscience
         Abstract | Full Text:  HTML   PDF (369K)

Unit 5.18 Using Phage Display in Neurobiology
         Abstract | Full Text:  HTML   PDF (268K)

Unit 6.14 Amperometry and Cyclic Voltammetry with Carbon Fiber Microelectrodes at Single Cells
         Abstract | Full Text:  HTML   PDF (323K)

Unit 7.20 Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid â-Peptides
         Abstract | Full Text:  HTML   PDF (143K)

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  Supplement 17, October 2001

Unit 8.14 Ultrasonic Vocalizations in Rat and Mouse Pups
         Abstract | Full Text:  HTML   PDF (197K)

Unit 8.15 Parental Behaviors in Rats and Mice
         Abstract | Full Text:  HTML   PDF (425K)

Unit 9.8 A Murine Model of Retroviral Encephalopathy
         Abstract | Full Text:  HTML   PDF (444K)

Unit 9.9 Animal Models of Retroviral Encephalopathies: Feline Model
         Abstract | Full Text:  HTML   PDF (368K)

Unit 9.10 Overview of Rodent Models for Obesity Research
         Abstract | Full Text:  HTML   PDF (213K)

Appendix 4C Animal Health Assurance
         Abstract | Full Text:  HTML   PDF (77K)

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  Supplement 16, August 2001

Unit 1.9 Detection of DNA Damage in Tissue Sections by In Situ Nick Translation
         Abstract | Full Text:  HTML   PDF (245K)

Unit 4.25 Overview of Nucleic Acid Arrays
         Abstract | Full Text:  HTML   PDF (300K)

Unit 4.26 Preparation of m RNA for Expression Monitoring
         Abstract | Full Text:  HTML   PDF (152K)

Unit 6.13 Synaptic Plasticity in the Hippocampal Slice Preparation
         Abstract | Full Text:  HTML   PDF (257K)

Unit 8.5C Cued and Contextual Fear Conditioning in Mice
         Full Text:  HTML   PDF (510K)

Unit 8.13 Latent Inhibition
         Abstract | Full Text:  HTML   PDF (272K)

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  Supplement 15, May 2001

Unit 1.8 Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH)
         Abstract | Full Text:  HTML   PDF (248K)

Unit 5.17 Analysis of RNA by Northern and Slot-Blot Hybridization
         Abstract | Full Text:  HTML   PDF (225K)

Unit 7.19 In Vivo Measurement of Blood-Brain Barrier Permeability
         Abstract | Full Text:  HTML   PDF (347K)

Unit 8.12 Motor Coordination and Balance in Rodents
         Abstract | Full Text:  HTML   PDF (263K)

Appendix 4B Mouse and Rat Anesthesia and Analgesia
           HTML   PDF (461K)

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  Supplement 14, February 2001

Unit 1.7 Targeted Toxins
         Abstract | Full Text:  HTML   PDF (391K)

Unit 5.15 Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics In Vivo
         Abstract | Full Text:  HTML   PDF (127K)

Unit 5.16 Green Fluorescent Protein in the Study of Neuronal Signaling Pathways
         Abstract | Full Text:  HTML   PDF (213K)

Unit 8.10A Rodent Models of Depression: Forced Swimming and Tail Suspension Behavioral Despair Tests in Rats and Mice
         Abstract | Full Text:  HTML   PDF (137K)

Unit 8.10B Rodent Models of Depression: Learned Helplessness Using a Triadic Design in Rats
         Abstract | Full Text:  HTML   PDF (306K)

Unit 8.10C Rodent Models of Depression: Learned Helplessness Induced in Mice
         Abstract | Full Text:  HTML   PDF (194K)

Unit 8.11A Fear-Potentiated Startle in Rats
         Abstract | Full Text:  HTML   PDF (239K)

Unit 9.7 Experimental Autoimmune Encephalomyelitis (EAE)
         Abstract | Full Text:  HTML   PDF (193K)

Appendix 4A High Precision Stereotaxic Surgery in Mice
         Abstract | Full Text:  HTML   PDF (139K)

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  Supplement 13, November 2000

Unit 4.22 Alphavirus-Mediated Gene Transfer into Neurons
           HTML   PDF (468K)

Unit 4.23 Gene Transfer into Neural Cells In Vitro Using Adenoviral Vectors
         Full Text:  HTML   PDF (495K)

Unit 4.24 Gene Transfer into Rat Brain Using Adenoviral Vectors
         Full Text:  HTML   PDF (1294K)

Unit 7.18 Assessment of Cell Viability in Primary Neuronal Cultures
         Full Text:  HTML   PDF (301K)

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  Supplement 12, August 2000

Unit 4.21 Production of High-Titer Lentiviral Vectors
         Full Text:  HTML   PDF (174K)

Unit 9.5 Rodent Models of Global Cerebral Ischemia
         Abstract | Full Text:  HTML   PDF (601K)

Unit 9.6 Rodent Models of Focal Cerebral Ischemia
         Abstract | Full Text:  HTML   PDF (413K)

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  Supplement 11, May 2000

Unit 1.6 Metabolic Mapping
         Abstract | Full Text:  HTML   PDF (408K)

Unit 4.20 Calcium Flux Assay in Xenopus Oocytes
         Abstract | Full Text:  HTML   PDF (114K)

Unit 5.14 Detection of Protein Phosphorylation in Tissues and Cells
         Abstract | Full Text:  HTML   PDF (266K)

Unit 7.17 Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
         Abstract | Full Text:  HTML   PDF (315K)

Unit 8.6G Macronutrient Selection in Experimental Animals
         Full Text:  HTML   PDF (257K)

Appendix 1L Introduction of Plasmid DNA into Cells
         Abstract | Full Text:  HTML   PDF (60K)

Appendix 1M Digestion of DNA with Restriction Endonucleases
         Abstract | Full Text:  HTML   PDF (134K)

Appendix 1N Agarose Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF (77K)

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  Supplement 10, February 2000

Unit 4.18 Overview of the Baculovirus Expression System
         Abstract | Full Text:  HTML   PDF (103K)

Unit 4.19 Protein Expression in the Baculovirus System
         Abstract | Full Text:  HTML   PDF (197K)

Unit 6.12 Voltage Clamp Recordings from Xenopus Oocytes
         Abstract | Full Text:  HTML   PDF (239K)

Unit 8.3 Animal Tests of Anxiety

Appendix 1F Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
         Abstract | Full Text:  HTML   PDF (305K)

Appendix 1J Preparation of Bacterial Plasmid DNA
         Abstract | Full Text:  HTML   PDF (137K)

Appendix 1K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
         Abstract | Full Text:  HTML   PDF (127K)

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  Supplement 9, November 1999

Unit 1.5 Transneuronal Circuit Analysis With Pseudorabies Viruses
         Abstract | Full Text:  HTML   PDF (1031K)

Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration
         Full Text:  HTML   PDF (302K)

Unit 6.11 Preparation and Maintenance of Organotypic Slice Cultures of CNS Tissue
         Abstract | Full Text:  HTML   PDF (883K)

Unit 7.16 Peripheral Type Benzodiazepine Binding Sites as a Tool for the Detection and Quantification of CNS Injury
         Abstract | Full Text:  HTML   PDF (139K)

Unit 9.4 Preclinical Models of Parkinson's Disease
         Abstract | Full Text:  HTML   PDF (905K)

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  Supplement 8, August 1999

Unit 3.17 Isolation and Purification of Primary Schwann Cells
         Abstract | Full Text:  HTML   PDF (128K)

Unit 4.2 PCR Cloning of Neural Gene Products
         Abstract | Full Text:  HTML   PDF (189K)

Unit 4.15 The Substituted-Cysteine Accessibility Method ( SCAM ) to Elucidate Membrane Protein Structure
         Abstract | Full Text:  HTML   PDF (146K)

Unit 4.16 Protein Expression in the Drosophila Schneider 2 Cell System
         Full Text:  HTML   PDF (170K)

Unit 5.13 Fluorescent In Situ Transcription in Cells and Tissues
         Abstract | Full Text:  HTML   PDF (204K)

Unit 9.3 Preclinical Models of Hepatic Encephalopathy
         Abstract | Full Text:  HTML   PDF (384K)

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  Supplement 7, May 1999

Unit 3.16 Gene Expression in Transgenic Mice Using Neural Promoters
         Abstract | Full Text:  HTML   PDF (216K)

Unit 5.12 The Use of Phage Display in Neurobiology
         Full Text:  HTML   PDF (280K)

Unit 6.10 Recording and Analyzing Synaptic Currents and Synaptic Potentials
         Abstract | Full Text:  HTML   PDF (209K)

Unit 7.15 Scintillation Proximity Assay
         Full Text:  HTML   PDF (553K)

Appendix 1I Preparation of RNA from Tissues and Cells
         Abstract | Full Text:  HTML   PDF (154K)

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  Supplement 6, February 1999

Unit 4.12 Overview of Gene Delivery into Cells Using HSV -1-Based Vectors
         Abstract | Full Text:  HTML   PDF (135K)

Unit 4.13 Generation of High-Titer Defective HSV-1 Vectors
         Abstract | Full Text:  HTML   PDF (213K)

Unit 4.14 Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
         Full Text:  HTML   PDF (551K)

Unit 7.14 Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
         Abstract | Full Text:  HTML   PDF (259K)

Unit 8.9 Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
         HTML   PDF (183K)

Unit 9.1 Dural Inflammation Model of Migraine Pain
         Abstract | Full Text:  HTML   PDF (148K)

Unit 9.2 Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
         Full Text:  HTML   PDF (154K)

Appendix 1F Liposome-Mediated Transfection

Appendix 1G Purification and Concentration of DNA from Aqueous Solutions
         Abstract | Full Text:  HTML   PDF (113K)

Appendix 1H Preparation of Genomic DNA from Mammalian Tissue
         Abstract | Full Text:  HTML   PDF (69K)

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  Supplement 5, November 1998

Unit 3.15 Neuronal Transfection Using Particle-Mediated Gene Transfer
         Abstract | Full Text:  HTML   PDF (695K)

Unit 5.10 Metal-Chelate Affinity Chromatography
         Abstract | Full Text:  HTML   PDF (178K)

Unit 5.11 Immunoaffinity Chromatography
         Abstract | Full Text:  HTML   PDF (149K)

Unit 6.9 Rapid Solution Application Methods
         Abstract | Full Text:  HTML   PDF (789K)

Unit 7.12 Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
         Abstract | Full Text:  HTML   PDF (139K)

Unit 7.13 Measurement of NO and NO Synthase
         Abstract | Full Text:  HTML   PDF (250K)

Unit 8.6E Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
         Full Text:  HTML   PDF (152K)

Unit 8.6F Conditioned Flavor Preferences: Evaluating Postingestive Reinforcement by Nutrients
         Abstract | Full Text:  HTML   PDF (173K)

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  Supplement 4, August 1998

Unit 3.14 Generation and Utilization of Phosphorylation State–Specific Antibodies to Investigate Signaling Pathways
         Abstract | Full Text:  HTML   PDF (379K)

Unit 7.10 Measurement of Chloride Movement in Neuronal Preparations
         Abstract | Full Text:  HTML   PDF (377K)

Unit 7.11 Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
         Abstract | Full Text:  HTML   PDF (226K)

Unit 8.5A Assessment of Spatial Memory Using the Radial Arm and Morris Water Mazes

Unit 8.5B Assessment of Spatial Memory Using the T Maze
         Abstract | Full Text:  HTML   PDF (117K)

Unit 8.6D Sham Feeding in Rats with Chronic, Reversible Gastric Fistulas
         Abstract | Full Text:  HTML   PDF (92K)

Unit 8.8 Measurement of Rodent Stereotyped Behavior
         Abstract | Full Text:  HTML   PDF (165K)

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  Supplement 3, May 1998

Unit 3.11 Calcium Phosphate Transfection of DNA into Neurons in Primary Culture
         Abstract | Full Text:  HTML   PDF (96K)

Unit 3.12 Purification of Oligodendrocytes and Their Progenitors Using Immunomagnetic Separation and Percoll Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (435K)

Unit 3.13 Isolation and Generation of Oligodendrocytes by Immunopanning
         Abstract | Full Text:  HTML   PDF (132K)

Unit 4.8 cDNA Expression Cloning in Mammalian Cells
         Abstract | Full Text:  HTML   PDF (245K)

Unit 4.9 Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships
         Abstract | Full Text:  HTML   PDF (98K)

Unit 4.10 Oligonucleotide-Directed Mutagenesis Without Phenotypic Selection
         Abstract | Full Text:  HTML   PDF (122K)

Unit 4.11 Directed Mutagenesis Using the Polymerase Chain Reaction
         Abstract | Full Text:  HTML   PDF (147K)

Unit 8.6A Overview of Methodological Approaches to the Study of Ingestive Behavior
         Abstract | Full Text:  HTML   PDF (95K)

Unit 8.6B Basic Measures of Food Intake
         Abstract | Full Text:  HTML   PDF (95K)

Unit 8.6C Direct, Continuous Behavioral Analysis of Drug Action on Feeding
         Full Text:  HTML   PDF (166K)

Unit 8.7 Measurement of Startle Response, Prepulse Inhibition, and Habituation
         Abstract | Full Text:  HTML   PDF (266K)

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  Supplement 2, February 1998

Unit 4.7 Transient Expression of Proteins Using COS Cells
         Abstract | Full Text:  HTML   PDF (132K)

Unit 5.9 Overview of Membrane Protein Solubilization
         Abstract | Full Text:  HTML   PDF (102K)

Unit 6.7 Patch-Pipet Recording in Brain Slices
         Abstract | Full Text:  HTML   PDF (143K)

Unit 6.8 Single-Channel Recording
         Abstract | Full Text:  HTML   PDF (362K)

Unit 7.7 Saturation Analysis of Ligand Binding Using a Centrifugation Procedure
         Abstract | Full Text:  HTML   PDF (96K)

Unit 7.8 Ligand Characterization Using Microphysiometry
         Abstract | Full Text:  HTML   PDF (385K)

Unit 7.9 Uptake and Release of Neurotransmitters
         Abstract | Full Text:  HTML   PDF (241K)

Appendix 3B Techniques for Mammalian Cell Tissue Culture
         Full Text:  HTML   PDF (159K)

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  Supplement 1, November 1997

Unit 3.9 Quantitative Analysis of In Vivo Cell Proliferation
         Full Text:  HTML   PDF (348K)

Unit 3.10 Experimental Transplantation in the Embryonic, Neonatal, and Adult Mammalian Brain
         HTML   PDF (1013K)

Unit 7.6 Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites
         Abstract | Full Text:  HTML   PDF (233K)

Unit 8.6 Overview of Methodological Approaches to the Study of Ingestive Behavior

Unit 8.7 Basic Measures of Food Intake

Appendix 1B Optimization of Transfection
         Abstract | Full Text:  HTML   PDF (83K)

Appendix 1C Calcium Phosphate Transfection
         Abstract | Full Text:  HTML   PDF (121K)

Appendix 1D Transfection Using DEAE -Dextran
         Abstract | Full Text:  HTML   PDF (135K)

Appendix 1E Transfection by Electroporation
         Abstract | Full Text:  HTML   PDF (89K)

Appendix 3A Preparation of an Iodinated Radioligand
         Abstract | Full Text:  HTML   PDF (104K)

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  Core Publication, September 1997

Unit 1.1 Basic Neuroanatomical Methods
         Abstract | Full Text:  HTML   PDF (116K)

Unit 1.2 Immunohistochemical Localization of Neurochemicals

Unit 1.3 Hybridization Histochemistry of Neural Transcripts

Unit 1.4 Receptor Binding Techniques
         Abstract | Full Text:  HTML   PDF (205K)

Unit 2.1 Theory and Application of Fluorescence Microscopy
         Full Text:  HTML   PDF (347K)

Unit 2.2 Basic Confocal Microscopy
         Abstract | Full Text:  HTML   PDF (436K)

Unit 2.3 Imaging Nervous System Activity
         Abstract | Full Text:  HTML   PDF (157K)

Unit 3.1 Culture of Neuroepithelial Stem Cells
         Abstract | Full Text:  HTML   PDF (90K)

Unit 3.2 Long-Term Culture of Hippocampal Neurons

Unit 3.3 Culture of Substantia Nigra Neurons
         Abstract | Full Text:  HTML   PDF (296K)

Unit 3.4 Isolation and Purification of Primary Oligodendrocyte Precursors
         Abstract | Full Text:  HTML   PDF (125K)

Unit 3.5 Isolation and Purification of Primary Rodent Astrocytes
         Abstract | Full Text:  HTML   PDF (181K)

Unit 3.6 Differentiation of Embryonic Stem Cells
         HTML   PDF (142K)

Unit 3.7 Immortalizing Central Nervous System Cells
         Abstract | Full Text:  HTML   PDF (184K)

Unit 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR
         Abstract | Full Text:  HTML   PDF (888K)

Unit 4.1 Homology Cloning of cDNA or Genomic DNA
         Abstract | Full Text:  HTML   PDF (97K)

Unit 4.2 PCR Cloning of Neural Gene Products

Unit 4.3 Expression Cloning of Neural Genes Using Xenopus laevis Oocytes
         Abstract | Full Text:  HTML   PDF (248K)

Unit 4.4 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
         Abstract | Full Text:  HTML   PDF (369K)

Unit 4.5 Overview of Neural Gene Expression
         Abstract | Full Text:  HTML   PDF (99K)

Unit 4.6 Selection of Transfected Mammalian Cells
         Abstract | Full Text:  HTML   PDF (244K)

Unit 5.1 RNA Analysis by Nuclease Protection

Unit 5.2 Differential Display of mRNA by PCR
         Abstract | Full Text:  HTML   PDF (268K)

Unit 5.3 Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System
         Abstract | Full Text:  HTML   PDF (258K)

Unit 5.4 Reducing Gene Expression in the Brain via Antisense Methods
         Abstract | Full Text:  HTML   PDF (176K)

Unit 5.5 Production of Polyclonal Antisera
         Abstract | Full Text:  HTML   PDF (140K)

Unit 5.6 Production of Antipeptide Antisera
         Abstract | Full Text:  HTML   PDF (227K)

Unit 5.7 Production of Antisera Using Fusion Proteins
         HTML   PDF (186K)

Unit 5.8 Epitope Tagging of Recombinant Proteins
         Abstract | Full Text:  HTML   PDF (161K)

Unit 6.1 The Electrophysiology Setup
         Abstract | Full Text:  HTML   PDF (109K)

Unit 6.2 Principles of Signal Conditioning
         Abstract | Full Text:  HTML   PDF (203K)

Unit 6.3 Fabrication of Patch Pipets

Unit 6.4 Preparation of Hippocampal Brain Slices
         Abstract | Full Text:  HTML   PDF (145K)

Unit 6.5 Acute Isolation of Neurons from the Mature Mammalian Central Nervous System
         Abstract | Full Text:  HTML   PDF (235K)

Unit 6.6 Whole-Cell Voltage Clamp Recording
         Abstract | Full Text:  HTML   PDF (343K)

Unit 7.1 Overview of Microdialysis
         HTML   PDF (315K)

Unit 7.2 Microdialysis in Rodents
         HTML   PDF (293K)

Unit 7.3 Microdialysis in Nonhuman Primates
         Abstract | Full Text:  HTML   PDF (461K)

Unit 7.4 Detection and Quantification of Neurotransmitters in Dialysates
         Abstract | Full Text:  HTML   PDF (214K)

Unit 7.5 Analyzing Radioligand Binding Data
         Full Text:  HTML   PDF (461K)

Unit 8.1 Locomotor Behavior
         HTML   PDF (133K)

Unit 8.2 Sexual and Reproductive Behaviors
         Abstract | Full Text:  HTML   PDF (247K)

Unit 8.3 Animal Tests of Anxiety

Unit 8.4 Application of Experimental Stressors in Laboratory Rodents
         HTML   PDF (166K)

Unit 8.5 Assessment of Spatial Memory Using the Radial Arm and Morris Water Mazes

Appendix 1A Molecular Biology References
         Abstract | Full Text:  HTML   PDF (67K)

Appendix 2A Common Stock Solutions, Buffers, and Media
         Abstract | Full Text:  HTML   PDF (107K)

Appendix 2B Standard Laboratory Equipment
         Abstract | Full Text:  HTML   PDF (62K)

Selected Suppliers of Reagents and Equipment
         Abstract | Full Text:  HTML   PDF (250K)

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