Current Protocols in Neuroscience
Online ISBN: 9780471142300
DOI: 10.1002/0471142301
Archive
Current Protocols in Neuroscience Supplements
2012: Supplement 59 Supplement 58
2011: Supplement 57 Supplement 56 Supplement 55 Supplement 54
2010: Supplement 53 Supplement 52 Supplement 51 Supplement 50
2009: Supplement 49 Supplement 48 Supplement 47 Supplement 46
2008: Supplement 45 Supplement 44 Supplement 43 Supplement 42
2007: Supplement 41 Supplement 40 Supplement 39 Supplement 38
2006: Supplement 37 Supplement 36 Supplement 35 Supplement 34
2005: Supplement 33 Supplement 32 Supplement 31 Supplement 30
2004: Supplement 29 Supplement 28 Supplement 27 Supplement 26
2003: Supplement 25 Supplement 24 Supplement 23 Supplement 22
2002: Supplement 21 Supplement 20 Supplement 19 Supplement 18
2001: Supplement 17 Supplement 16 Supplement 15 Supplement 14
2000: Supplement 13 Supplement 12 Supplement 11 Supplement 10
1999: Supplement 9 Supplement 8 Supplement 7 Supplement 6
1998: Supplement 5 Supplement 4 Supplement 3 Supplement 2
1997: Supplement 1 Core publication
Supplement 59, April 2012
Unit 1.20 Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice
Abstract | Full Text: HTML PDF
Unit 2.7 Characterizing Synaptic Vesicle Proteins Using Synaptosomal Fractions and Cultured Hippocampal Neurons
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Unit 3.25 Culturing Embryonic Nasal Explants for Developmental and Physiological Study
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Unit 5.30 A Protein Cross-Linking Assay for Measuring Cell Surface Expression of Glutamate Receptor Subunits in the Rodent Brain After In Vivo Treatments
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Unit 9.38 Diet-Induced Models of Obesity (DIO) in Rodents
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Supplement 58, January 2012
Unit 2.15 Confocal Laser Scanning Microscopic Photoconversion: A New Method to Stabilize Fluorescently Labeled Cellular Elements for Electron Microscope Analysis
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Unit 2.16 Use of Channelrhodopsin for Activation of CNS Neurons
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Unit 3.24 Culture of Mouse Olfactory Sensory Neurons
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Unit 8.28 Analysis of Skilled Forelimb Movement in Rats: The Single Pellet Reaching Test and Staircase Test
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Unit 9.37 Pentylenetetrazol (PTZ) Kindling Model of Epilepsy
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Unit 10.5 A Rubric for Extracting Idea Density from Oral Language Samples
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Supplement 57, October 2011
Unit 2.14 High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy
Abstract | Full Text: HTML PDF
Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration
Abstract | Full Text: HTML PDF
Unit 4.22 Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
Abstract | Full Text: HTML PDF
Unit 8.27 A Mouse Model for Observational Fear Learning and the Empathetic Response
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Appendix 1E Transfection by Electroporation
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Supplement 56, July 2011
Unit 2.2 Basic Confocal Microscopy
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Unit 3.23 In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
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Unit 8.26 Automated Three-Chambered Social Approach Task for Mice
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Appendix 1K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
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Supplement 55, April 2011
Unit 2.13 DiOlistic Labeling in Fixed Brain Slices: Phenotype, Morphology, and Dendritic Spines
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Unit 4.4 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
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Unit 4.30 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
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Unit 8.10A Rodent Models of Depression: Forced Swim and Tail Suspension Behavioral Despair Tests in Rats and Mice
Abstract | Full Text: HTML PDF
Supplement 54, January 2011
Unit 6.13 Synaptic Plasticity in the Hippocampal Slice Preparation
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Unit 9.35 Overview of Animal Models of Attention Deficit Hyperactivity Disorder (ADHD)
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Unit 9.36 Open-Space Forced Swim Model of Depression for Mice
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Unit 10.4 Clinical and Neuroimaging Assessments for Research Studies (Including Drug Trials) in Multiple Sclerosis
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Supplement 53, October 2010
Unit 3.22 A Method for Preparing Primary Retinal Cell Cultures for Evaluating the Neuroprotective and Neuritogenic Effect of Factors on Axotomized Mature CNS Neurons
Abstract | Full Text: HTML PDF
Unit 4.21 Production and Titration of Lentiviral Vectors
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Unit 8.5I Social Recognition Assay in the Rat
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Unit 8.25 Modeling Appetitive Pavlovian-Instrumental Interactions in Mice
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Unit 9.34 Drug-Induced Sexual Dysfunction in Rats
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Supplement 52, July 2010
Unit 6.25 Electroencephalography (EEG) and Event-Related Potentials (ERPs) with Human Participants
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Unit 7.5 Analyzing Binding Data
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Unit 7.22 Isolation of Mitochondria from the CNS
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Unit 9.33 A Murine Model of Atypical Antipsychotic-Induced Weight Gain and Metabolic Dysregulation
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Supplement 51, April 2010
Unit 4.34 Transfection of Mouse Cochlear Explants by Electroporation
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Unit 5.12 The Use of Phage Display in Neurobiology
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Unit 5.29 Visualization of G Protein–Coupled Receptor (GPCR) Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC)
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Unit 9.12 Operant Self-Administration Models for Testing the Neuropharmacological Basis of Ethanol Consumption in Rats
Abstract | Full Text: HTML PDF
Supplement 50, January 2010
Unit 1.19 Mapping Lineage Using BAC-Cre Reporter Lines
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Unit 2.1 Fluorescence Microscopy: A Concise Guide to Current Imaging Methods
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Unit 2.5 Leading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
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Unit 4.24 Gene Transfer into Rat Brain Using Adenoviral Vectors
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Unit 4.33 Generation of Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) Using Directed Molecular Evolution
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Supplement 49, October 2009
Unit 2.3 Imaging Nervous System Activity
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Unit 6.24 EEG Recording in Rodents, with a Focus on Epilepsy
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Unit 9.32 Recapitulation and Reversal of a Persistent Depression-like Syndrome in Rodents
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Unit 10.1 Assessment of Parkinson Disease Manifestations
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Unit 10.2 EEG Recording and Analysis for Sleep Research
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Unit 10.3 Neuropsychological Assessment in the Diagnosis of Alzheimer's Disease
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Supplement 48, July 2009
Unit 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR
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Unit 5.5 Production of Polyclonal Antisera
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Unit 7.4 Detection and Quantification of Neurotransmitters in Dialysates
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Unit 7.17 Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
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Unit 8.24 Simple Behavioral Assessment of Mouse Olfaction
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Appendix 4I Assessing Reproductive Status/Stages in Mice
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Supplement 47, April 2009
Unit 3.6 Differentiation of Embryonic Stem Cells
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Unit 4.32 Transfection of Cultured Primary Neurons via Nucleofection
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Unit 7.1 Overview of Brain Microdialysis
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Unit 7.2 Microdialysis in Rodents
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Unit 8.23 Fear Extinction in Rodents
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Supplement 46, January 2009
Unit 1.18 Evolution of Neurotoxins: From Research Modalities to Clinical Realities
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Unit 5.28 Identifying Novel Protein-Protein Interactions Using Co-Immunoprecipitation and Mass Spectroscopy
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Unit 9.31 An Animal Model of Premenstrual Dysphoric Disorder Sensitive to Antidepressants
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Supplement 45, October 2008
Unit 2.12 The Importance of Titrating Antibodies for Immunocytochemical Methods
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Unit 4.23 Gene Transfer into Neural Cells In Vitro Using Adenoviral Vectors
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Unit 4.28 Gene Expression Analysis of Neural Cells and Tissues Using DNA Microarrays
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Unit 9.30 Animal Models of Obsessive-Compulsive Disorder
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Supplement 44, July 2008
Unit 3.21 Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer
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Unit 4.31 Conditional Gene Expression and Targeting in Neuroscience Research
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Unit 7.18 Assessment of Cell Viability in Primary Neuronal Cultures
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Unit 9.28 The Majchrowicz Binge Alcohol Protocol: An Intubation Technique to Study Alcohol Dependence in Rats
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Unit 9.29 Vapor Inhalation of Alcohol in Rats
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Supplement 43, April 2008
Unit 1.17 Retrograde Axonal Tracing with Fluorescent Markers
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Unit 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function
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Unit 2.11 Ballistic Labeling with Fluorescent Dyes and Indicators
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Unit 3.20 Dissection and Culture of Embryonic Spinal Commissural Neurons
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Unit 9.27 Schedule-Induced Polydipsia: A Rat Model of Obsessive-Compulsive Disorder
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Supplement 42, January 2008
Unit 1.16 Biochemical Fractionation of Brain Tissue for Studies of Receptor Distribution and Trafficking
Abstract | Full Text: HTML PDF (421K)
Unit 3.10 Neural Stem Cell Transplantation in Mouse Brain
Abstract | Full Text: HTML PDF (599K)
Unit 9.26 Overview of Mouse Assays of Ethanol Intoxication
Abstract | Full Text: HTML PDF (241K)
Appendix 4B Mouse and Rat Anesthesia and Analgesia
Abstract | Full Text: HTML PDF (693K)
Supplement 41, October 2007
Unit 4.22 Alphaviruses: Semliki Forest Virus and Sindbis Virus Vectors for Gene Transfer into Neurons
Abstract | Full Text: HTML PDF (670K)
Unit 5.27 Split-Ubiquitin System for Identifying Protein-Protein Interactions in Membrane and Full-Length Proteins
Abstract | Full Text: HTML PDF (1219K)
Unit 8.9 Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
Abstract | Full Text: HTML PDF (322K)
Unit 9.25 Ratings of L-DOPA-Induced Dyskinesia in the Unilateral 6-OHDA Lesion Model of Parkinson's Disease in Rats and Mice
Abstract | Full Text: HTML PDF (640K)
Supplement 40, July 2007
Unit 4.14 Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
Abstract | Full Text: HTML PDF (414K)
Unit 4.29 Overview of Gene Targeting by Homologous Recombination
Abstract | Full Text: HTML PDF (680K)
Unit 5.7 Production of Antisera Using Fusion Proteins
Abstract | Full Text: HTML PDF (253K)
Unit 8.1 Locomotor Behavior
Abstract | Full Text: HTML PDF (195K)
Supplement 39, April 2007
Unit 1.15 Histochemical Methods for the Detection of Apoptosis in the Nervous System
Abstract | Full Text: HTML PDF (277K)
Unit 5.26 Lentiviral Strategies for RNAi Knockdown of Neuronal Genes
Abstract | Full Text: HTML PDF (362K)
Unit 8.22 Delay Discounting and Impulsive Choice in the Rat
Abstract | Full Text: HTML PDF (701K)
Unit 9.24 Overview of Animal Models of Schizophrenia
Abstract | Full Text: HTML PDF (176K)
Supplement 38, January 2007
Unit 6.22 Whole-Cell Recording In Vivo
Abstract | Full Text: HTML PDF (142K)
Unit 6.23 Electrophysiological Recordings from Neonatal Neocortical Brain Slices
Abstract | Full Text: HTML PDF (181K)
Unit 7.21 Assays for Cyclic Nucleotide-Specific Phosphodiesterases (PDEs) in the Central Nervous System (PDE1, PDE2, PDE4, and PDE10)
Abstract | Full Text: HTML PDF (271K)
Appendix 3B Techniques for Mammalian Cell Tissue Culture
Abstract | Full Text: HTML PDF (228K)
Supplement 37, October 2006
Unit 1.14 Anterograde Axonal Tract Tracing
Abstract | Full Text: HTML PDF (440K)
Unit 3.9 Quantitative Analysis of In Vivo Cell Proliferation
Abstract | Full Text: HTML PDF (317K)
Unit 4.21 Production and Titration of Lentiviral Vectors
Abstract | Full Text: HTML PDF (829K)
Unit 6.20 Caged Molecules: Principles and Practical Considerations
Abstract | Full Text: HTML PDF (854K)
Unit 6.21 Photolysis of Caged Neurotransmitters: Theory and Procedures for Light Delivery
Abstract | Full Text: HTML PDF (323K)
Supplement 36, July 2006
Unit 2.10 Whole-Mount Immunohistochemistry of the Brain
Abstract | Full Text: HTML PDF (323K)
Unit 3.19 Rodent Sensory Neuron Culture and Analysis
Abstract | Full Text: HTML PDF (244K)
Unit 9.23A Binge Eating in Rats Produced by Combining Dieting with Stress
Abstract | Full Text: HTML PDF (226K)
Unit 9.23B Binge Eating in Rats with Limited Access to Vegetable Shortening
Abstract | Full Text: HTML PDF (163K)
Unit 9.23C Sugar Bingeing in Rats
Abstract | Full Text: HTML PDF (153K)
Unit 9.23D Characterization of Energy Expenditure in Rodents by Indirect Calorimetry
Abstract | Full Text: HTML PDF (342K)
Supplement 35, April 2006
Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use for In Vitro and In Vivo Administration
Abstract | Full Text: HTML PDF (591K)
Unit 5.24 Immunoprecipitation
Abstract | Full Text: HTML PDF (525K)
Unit 5.25 Detection of Protein-Protein Interactions by Coprecipitation
Abstract | Full Text: HTML PDF (261K)
Supplement 34, January 2006
Unit 5.22 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
Abstract | Full Text: HTML PDF (335K)
Unit 5.23 Monitoring Protein-Protein Interactions in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
Abstract | Full Text: HTML PDF (274K)
Unit 8.4 Application of Experimental Stressors in Laboratory Rodents
Abstract | Full Text: HTML PDF (144K)
Unit 8.21A Overview of Methods for Assessing the Mouse Auditory System
Abstract | Full Text: HTML PDF (39K)
Unit 8.21B Measurement of the Auditory Brainstem Response (ABR) to Study Auditory Sensitivity in Mice
Abstract | Full Text: HTML PDF (116K)
Unit 8.21C Assessment of Cochlear Function in Mice: Distortion-Product Otoacoustic Emissions
Abstract | Full Text: HTML PDF (550K)
Unit 8.21D Behavioral Assessment of Hearing in Mice—Conditioned Suppression
Abstract | Full Text: HTML PDF (231K)
Supplement 33, October 2005
Unit 3.18 Fluorescence-Based Sorting of Neural Stem Cells and Progenitors
Abstract | Full Text: HTML PDF (1025K)
Unit 8.20 Olfactometry with Mice
Abstract | Full Text: HTML PDF (586K)
Unit 9.22 Overview on Rodent Models of Alzheimer's Disease
Abstract | Full Text: HTML PDF (149K)
Appendix 4F Parenteral Injections
Abstract | Full Text: HTML PDF (137K)
Appendix 4G Blood Collection
Abstract | Full Text: HTML PDF (112K)
Appendix 4H Euthanasia
Abstract | Full Text: HTML PDF (44K)
Supplement 32, July 2005
Unit 2.8 3-D Reconstruction of Neurons from Multichannel Confocal Laser Scanning Image Series
Abstract | Full Text: HTML PDF (899K)
Unit 2.9 Two-Photon Imaging in Live Rodents
Abstract | Full Text: HTML PDF (707K)
Unit 9.20 Chronic Intravenous Drug Self-Administration in Rats and Mice
Abstract | Full Text: HTML PDF (1482K)
Unit 9.21 Intravenous Self-Administration Techniques in Monkeys
Abstract | Full Text: HTML PDF (241K)
Supplement 31, April 2005
Unit 5.20 An Overview on the Generation of BAC Transgenic Mice for Neuroscience Research
Abstract | Full Text: HTML PDF (158K)
Unit 5.21 Modification of Bacterial Artificial Chromosomes (BACs) and Preparation of Intact BAC DNA for Generation of Transgenic Mice
Abstract | Full Text: HTML PDF (227K)
Unit 8.5H Assaying Aspects of Attention and Impulse Control in Mice Using the 5-Choice Serial Reaction Time Task
Abstract | Full Text: HTML PDF (348K)
Unit 9.19 Chemoconvulsant Model of Chronic Spontaneous Seizures
Abstract | Full Text: HTML PDF (127K)
Supplement 30, January 2005
Unit 7.15 Scintillation Proximity Assay
Abstract | Full Text: HTML PDF (843K)
Unit 8.19 Defensive Responses to Predator Threat in the Rat and Mouse
Abstract | Full Text: HTML PDF (352K)
Supplement 29, October 2004
Unit 5.19 Immunoblotting and Immunodetection
Abstract | Full Text: HTML PDF (304K)
Unit 6.19 Patch-Clamp Recording from Neuronal Dendrites
Abstract | Full Text: HTML PDF (287K)
Unit 8.6C Direct, Continuous Behavioral Analysis of Drug Action on Feeding
Abstract | Full Text: HTML PDF (116K)
Unit 8.6E Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
Abstract | Full Text: HTML PDF (120K)
Unit 9.18 Animal Models of Painful Diabetic Neuropathy: The STZ Rat Model
Abstract | Full Text: HTML PDF (104K)
Supplement 28, July 2004
Unit 2.7 Multidisciplinary Approaches for Characterizing Synaptic Vesicle Proteins
Abstract | Full Text: HTML PDF (249K)
Unit 8.5G Social Transmission of Food Preference in Mice
Abstract | Full Text: HTML PDF (138K)
Unit 9.2 Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
Abstract | Full Text: HTML PDF (338K)
Appendix 5B Databases and Web Sites for Neurogenetics
Abstract | Full Text: HTML PDF (54K)
Supplement 27, April 2004
Unit 1.13 Quantified Assessment of Terminal Density and Innervation
Abstract | Full Text: HTML PDF (355K)
Unit 4.16 Protein Expression in the Drosophila Schneider 2 Cell System
Abstract | Full Text: HTML PDF (173K)
Unit 8.5C Cued and Contextual Fear Conditioning in Mice
Abstract | Full Text: HTML PDF (509K)
Unit 8.6G Macronutrient Selection in Experimental Animals
Abstract | Full Text: HTML PDF (246K)
Appendix 4D Handling and Restraint
Abstract | Full Text: HTML PDF (248K)
Appendix 4E Animal Identification
Abstract | Full Text: HTML PDF (94K)
Supplement 26, January 2004
Unit 1.12 Intracellular and Juxtacellular Staining with Biocytin
Abstract | Full Text: HTML PDF (222K)
Unit 3.2 Long-Term Culture of Hippocampal Neurons
Abstract | Full Text: HTML PDF (359K)
Unit 6.3 Fabrication of Patch Pipets
Abstract | Full Text: HTML PDF (1035K)
Unit 8.3 Animal Tests of Anxiety
Abstract | Full Text: HTML PDF (238K)
Unit 8.5A Assessment of Spatial Memory Using the Radial Arm Maze and Morris Water Maze
Abstract | Full Text: HTML PDF (170K)
Supplement 25, October 2003
Unit 1.2 Immunohistochemical Localization of Proteins in the Nervous System
Abstract | Full Text: HTML PDF (179K)
Unit 1.3 Hybridization Histochemistry of Neural Transcripts
Abstract | Full Text: HTML PDF (249K)
Unit 6.18 Recording in the Cerebellar Slice
Abstract | Full Text: HTML PDF (215K)
Unit 8.5F Assessment of Learning and Memory Using the Autoshaping of Operant Responding in Mice
Abstract | Full Text: HTML PDF (249K)
Unit 8.18 Assessment of Developmental Milestones in Rodents
Abstract | Full Text: HTML PDF (186K)
Supplement 24, July 2003
Unit 1.11 Counting Cells in Sectioned Material: A Suite of Techniques, Tools, and Tips
Abstract | Full Text: HTML PDF (312K)
Unit 8.17 Assessment of Murine Startle Reactivity, Prepulse Inhibition, and Habituation
Abstract | Full Text: HTML PDF (383K)
Unit 9.17 Measurement of Panic-Like Responses Following Intravenous Infusion of Sodium Lactate in Panic-Prone Rats
Abstract | Full Text: HTML PDF (526K)
Supplement 23, April 2003
Unit 1.10 Quantitative In Situ Hybridization for the Study of Gene Expression at the Regional and Cellular Levels
Abstract | Full Text: HTML PDF (289K)
Unit 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function
Full Text: HTML PDF (211K)
Unit 5.1 RNA Analysis by Nuclease Protection
Abstract | Full Text: HTML PDF (594K)
Unit 6.17 Imaging Nervous System Activity with Voltage-Sensitive Dyes
Abstract | Full Text: HTML PDF (403K)
Unit 9.16 Inducing Photochemical Cortical Lesions in Rat Brain
Abstract | Full Text: HTML PDF (368K)
Supplement 22, January 2003
Unit 8.5E Assessment of Sustained and Divided Attention in Rats
Abstract | Full Text: HTML PDF (167K)
Unit 8.16 Mouse Social Recognition and Preference
Abstract | Full Text: HTML PDF (451K)
Unit 9.14 Models of Neuropathic Pain in the Rat
Abstract | Full Text: HTML PDF (225K)
Unit 9.15 Place Preference Test in Rodents
Abstract | Full Text: HTML PDF (318K)
Appendix 5A Internet Resources for Genomic, Bioinformatics, and Medical Genetics Information
Abstract | Full Text: HTML PDF (68K)
Supplement 21, October 2002
Unit 4.29 Overview of Gene Targeting by Homologous Recombination
Full Text: HTML PDF (163K)
Unit 4.30 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
Abstract | Full Text: HTML PDF (166K)
Unit 6.16 Chronic Recording of Extracellular Neuronal Activity in Behaving Animals
Abstract | Full Text: HTML PDF (278K)
Unit 8.5D Social Learning of Food Preferences in Rodents: Rapid Appetitive Learning
Abstract | Full Text: HTML PDF (124K)
Supplement 20, July 2002
Unit 2.5 Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
Full Text: HTML PDF (198K)
Unit 4.28 Gene Expression Analysis Using c DNA Microarrays
Full Text: HTML PDF (273K)
Unit 9.13 Models of Amyotrophic Lateral Sclerosis
Abstract | Full Text: HTML PDF (418K)
Supplement 19, April 2002
Unit 4.27 cDNA Library Construction from Single Cells
Abstract | Full Text: HTML PDF (230K)
Unit 6.15 Preparation and Maintenance of Organotypic Cultures for Multi-Electrode Array Recordings
Abstract | Full Text: HTML PDF (194K)
Unit 8.11B Fear-Potentiated Startle in Mice
Abstract | Full Text: HTML PDF (301K)
Unit 9.11 Intravenous Self-Administration of Ethanol in Mice
Abstract | Full Text: HTML PDF (185K)
Unit 9.12 Preclinical Models to Evaluate Potential Pharmacotherapeutic Agents in Treating Alcoholism and Studying the Neuropharmacological Bases of Ethanol-Seeking Behaviors in Rats
Full Text: HTML PDF (354K)
Supplement 18, February 2002
Unit 2.4 Electronic Imaging in Neuroscience
Abstract | Full Text: HTML PDF (369K)
Unit 5.18 Using Phage Display in Neurobiology
Abstract | Full Text: HTML PDF (268K)
Unit 6.14 Amperometry and Cyclic Voltammetry with Carbon Fiber Microelectrodes at Single Cells
Abstract | Full Text: HTML PDF (323K)
Unit 7.20 Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid â-Peptides
Abstract | Full Text: HTML PDF (143K)
Supplement 17, October 2001
Unit 8.14 Ultrasonic Vocalizations in Rat and Mouse Pups
Abstract | Full Text: HTML PDF (197K)
Unit 8.15 Parental Behaviors in Rats and Mice
Abstract | Full Text: HTML PDF (425K)
Unit 9.8 A Murine Model of Retroviral Encephalopathy
Abstract | Full Text: HTML PDF (444K)
Unit 9.9 Animal Models of Retroviral Encephalopathies: Feline Model
Abstract | Full Text: HTML PDF (368K)
Unit 9.10 Overview of Rodent Models for Obesity Research
Abstract | Full Text: HTML PDF (213K)
Appendix 4C Animal Health Assurance
Abstract | Full Text: HTML PDF (77K)
Supplement 16, August 2001
Unit 1.9 Detection of DNA Damage in Tissue Sections by In Situ Nick Translation
Abstract | Full Text: HTML PDF (245K)
Unit 4.25 Overview of Nucleic Acid Arrays
Abstract | Full Text: HTML PDF (300K)
Unit 4.26 Preparation of m RNA for Expression Monitoring
Abstract | Full Text: HTML PDF (152K)
Unit 6.13 Synaptic Plasticity in the Hippocampal Slice Preparation
Abstract | Full Text: HTML PDF (257K)
Unit 8.5C Cued and Contextual Fear Conditioning in Mice
Full Text: HTML PDF (510K)
Unit 8.13 Latent Inhibition
Abstract | Full Text: HTML PDF (272K)
Supplement 15, May 2001
Unit 1.8 Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH)
Abstract | Full Text: HTML PDF (248K)
Unit 5.17 Analysis of RNA by Northern and Slot-Blot Hybridization
Abstract | Full Text: HTML PDF (225K)
Unit 7.19 In Vivo Measurement of Blood-Brain Barrier Permeability
Abstract | Full Text: HTML PDF (347K)
Unit 8.12 Motor Coordination and Balance in Rodents
Abstract | Full Text: HTML PDF (263K)
Appendix 4B Mouse and Rat Anesthesia and Analgesia
HTML PDF (461K)
Supplement 14, February 2001
Unit 1.7 Targeted Toxins
Abstract | Full Text: HTML PDF (391K)
Unit 5.15 Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics In Vivo
Abstract | Full Text: HTML PDF (127K)
Unit 5.16 Green Fluorescent Protein in the Study of Neuronal Signaling Pathways
Abstract | Full Text: HTML PDF (213K)
Unit 8.10A Rodent Models of Depression: Forced Swimming and Tail Suspension Behavioral Despair Tests in Rats and Mice
Abstract | Full Text: HTML PDF (137K)
Unit 8.10B Rodent Models of Depression: Learned Helplessness Using a Triadic Design in Rats
Abstract | Full Text: HTML PDF (306K)
Unit 8.10C Rodent Models of Depression: Learned Helplessness Induced in Mice
Abstract | Full Text: HTML PDF (194K)
Unit 8.11A Fear-Potentiated Startle in Rats
Abstract | Full Text: HTML PDF (239K)
Unit 9.7 Experimental Autoimmune Encephalomyelitis (EAE)
Abstract | Full Text: HTML PDF (193K)
Appendix 4A High Precision Stereotaxic Surgery in Mice
Abstract | Full Text: HTML PDF (139K)
Supplement 13, November 2000
Unit 4.22 Alphavirus-Mediated Gene Transfer into Neurons
HTML PDF (468K)
Unit 4.23 Gene Transfer into Neural Cells In Vitro Using Adenoviral Vectors
Full Text: HTML PDF (495K)
Unit 4.24 Gene Transfer into Rat Brain Using Adenoviral Vectors
Full Text: HTML PDF (1294K)
Unit 7.18 Assessment of Cell Viability in Primary Neuronal Cultures
Full Text: HTML PDF (301K)
Supplement 12, August 2000
Unit 4.21 Production of High-Titer Lentiviral Vectors
Full Text: HTML PDF (174K)
Unit 9.5 Rodent Models of Global Cerebral Ischemia
Abstract | Full Text: HTML PDF (601K)
Unit 9.6 Rodent Models of Focal Cerebral Ischemia
Abstract | Full Text: HTML PDF (413K)
Supplement 11, May 2000
Unit 1.6 Metabolic Mapping
Abstract | Full Text: HTML PDF (408K)
Unit 4.20 Calcium Flux Assay in Xenopus Oocytes
Abstract | Full Text: HTML PDF (114K)
Unit 5.14 Detection of Protein Phosphorylation in Tissues and Cells
Abstract | Full Text: HTML PDF (266K)
Unit 7.17 Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
Abstract | Full Text: HTML PDF (315K)
Unit 8.6G Macronutrient Selection in Experimental Animals
Full Text: HTML PDF (257K)
Appendix 1L Introduction of Plasmid DNA into Cells
Abstract | Full Text: HTML PDF (60K)
Appendix 1M Digestion of DNA with Restriction Endonucleases
Abstract | Full Text: HTML PDF (134K)
Appendix 1N Agarose Gel Electrophoresis
Abstract | Full Text: HTML PDF (77K)
Supplement 10, February 2000
Unit 4.18 Overview of the Baculovirus Expression System
Abstract | Full Text: HTML PDF (103K)
Unit 4.19 Protein Expression in the Baculovirus System
Abstract | Full Text: HTML PDF (197K)
Unit 6.12 Voltage Clamp Recordings from Xenopus Oocytes
Abstract | Full Text: HTML PDF (239K)
Unit 8.3 Animal Tests of Anxiety
Appendix 1F Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
Abstract | Full Text: HTML PDF (305K)
Appendix 1J Preparation of Bacterial Plasmid DNA
Abstract | Full Text: HTML PDF (137K)
Appendix 1K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract | Full Text: HTML PDF (127K)
Supplement 9, November 1999
Unit 1.5 Transneuronal Circuit Analysis With Pseudorabies Viruses
Abstract | Full Text: HTML PDF (1031K)
Unit 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration
Full Text: HTML PDF (302K)
Unit 6.11 Preparation and Maintenance of Organotypic Slice Cultures of CNS Tissue
Abstract | Full Text: HTML PDF (883K)
Unit 7.16 Peripheral Type Benzodiazepine Binding Sites as a Tool for the Detection and Quantification of CNS Injury
Abstract | Full Text: HTML PDF (139K)
Unit 9.4 Preclinical Models of Parkinson's Disease
Abstract | Full Text: HTML PDF (905K)
Supplement 8, August 1999
Unit 3.17 Isolation and Purification of Primary Schwann Cells
Abstract | Full Text: HTML PDF (128K)
Unit 4.2 PCR Cloning of Neural Gene Products
Abstract | Full Text: HTML PDF (189K)
Unit 4.15 The Substituted-Cysteine Accessibility Method ( SCAM ) to Elucidate Membrane Protein Structure
Abstract | Full Text: HTML PDF (146K)
Unit 4.16 Protein Expression in the Drosophila Schneider 2 Cell System
Full Text: HTML PDF (170K)
Unit 5.13 Fluorescent In Situ Transcription in Cells and Tissues
Abstract | Full Text: HTML PDF (204K)
Unit 9.3 Preclinical Models of Hepatic Encephalopathy
Abstract | Full Text: HTML PDF (384K)
Supplement 7, May 1999
Unit 3.16 Gene Expression in Transgenic Mice Using Neural Promoters
Abstract | Full Text: HTML PDF (216K)
Unit 5.12 The Use of Phage Display in Neurobiology
Full Text: HTML PDF (280K)
Unit 6.10 Recording and Analyzing Synaptic Currents and Synaptic Potentials
Abstract | Full Text: HTML PDF (209K)
Unit 7.15 Scintillation Proximity Assay
Full Text: HTML PDF (553K)
Appendix 1I Preparation of RNA from Tissues and Cells
Abstract | Full Text: HTML PDF (154K)
Supplement 6, February 1999
Unit 4.12 Overview of Gene Delivery into Cells Using HSV -1-Based Vectors
Abstract | Full Text: HTML PDF (135K)
Unit 4.13 Generation of High-Titer Defective HSV-1 Vectors
Abstract | Full Text: HTML PDF (213K)
Unit 4.14 Gene Delivery Using Helper Virus–Free HSV-1 Amplicon Vectors
Full Text: HTML PDF (551K)
Unit 7.14 Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
Abstract | Full Text: HTML PDF (259K)
Unit 8.9 Models of Nociception: Hot-Plate, Tail-Flick, and Formalin Tests in Rodents
HTML PDF (183K)
Unit 9.1 Dural Inflammation Model of Migraine Pain
Abstract | Full Text: HTML PDF (148K)
Unit 9.2 Traumatic Brain Injury in the Rat Using the Fluid-Percussion Model
Full Text: HTML PDF (154K)
Appendix 1F Liposome-Mediated Transfection
Appendix 1G Purification and Concentration of DNA from Aqueous Solutions
Abstract | Full Text: HTML PDF (113K)
Appendix 1H Preparation of Genomic DNA from Mammalian Tissue
Abstract | Full Text: HTML PDF (69K)
Supplement 5, November 1998
Unit 3.15 Neuronal Transfection Using Particle-Mediated Gene Transfer
Abstract | Full Text: HTML PDF (695K)
Unit 5.10 Metal-Chelate Affinity Chromatography
Abstract | Full Text: HTML PDF (178K)
Unit 5.11 Immunoaffinity Chromatography
Abstract | Full Text: HTML PDF (149K)
Unit 6.9 Rapid Solution Application Methods
Abstract | Full Text: HTML PDF (789K)
Unit 7.12 Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
Abstract | Full Text: HTML PDF (139K)
Unit 7.13 Measurement of NO and NO Synthase
Abstract | Full Text: HTML PDF (250K)
Unit 8.6E Conditioned Flavor Aversions: Assessment of Drug-Induced Suppression of Food Intake
Full Text: HTML PDF (152K)
Unit 8.6F Conditioned Flavor Preferences: Evaluating Postingestive Reinforcement by Nutrients
Abstract | Full Text: HTML PDF (173K)
Supplement 4, August 1998
Unit 3.14 Generation and Utilization of Phosphorylation State–Specific Antibodies to Investigate Signaling Pathways
Abstract | Full Text: HTML PDF (379K)
Unit 7.10 Measurement of Chloride Movement in Neuronal Preparations
Abstract | Full Text: HTML PDF (377K)
Unit 7.11 Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
Abstract | Full Text: HTML PDF (226K)
Unit 8.5A Assessment of Spatial Memory Using the Radial Arm and Morris Water Mazes
Unit 8.5B Assessment of Spatial Memory Using the T Maze
Abstract | Full Text: HTML PDF (117K)
Unit 8.6D Sham Feeding in Rats with Chronic, Reversible Gastric Fistulas
Abstract | Full Text: HTML PDF (92K)
Unit 8.8 Measurement of Rodent Stereotyped Behavior
Abstract | Full Text: HTML PDF (165K)
Supplement 3, May 1998
Unit 3.11 Calcium Phosphate Transfection of DNA into Neurons in Primary Culture
Abstract | Full Text: HTML PDF (96K)
Unit 3.12 Purification of Oligodendrocytes and Their Progenitors Using Immunomagnetic Separation and Percoll Gradient Centrifugation
Abstract | Full Text: HTML PDF (435K)
Unit 3.13 Isolation and Generation of Oligodendrocytes by Immunopanning
Abstract | Full Text: HTML PDF (132K)
Unit 4.8 cDNA Expression Cloning in Mammalian Cells
Abstract | Full Text: HTML PDF (245K)
Unit 4.9 Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships
Abstract | Full Text: HTML PDF (98K)
Unit 4.10 Oligonucleotide-Directed Mutagenesis Without Phenotypic Selection
Abstract | Full Text: HTML PDF (122K)
Unit 4.11 Directed Mutagenesis Using the Polymerase Chain Reaction
Abstract | Full Text: HTML PDF (147K)
Unit 8.6A Overview of Methodological Approaches to the Study of Ingestive Behavior
Abstract | Full Text: HTML PDF (95K)
Unit 8.6B Basic Measures of Food Intake
Abstract | Full Text: HTML PDF (95K)
Unit 8.6C Direct, Continuous Behavioral Analysis of Drug Action on Feeding
Full Text: HTML PDF (166K)
Unit 8.7 Measurement of Startle Response, Prepulse Inhibition, and Habituation
Abstract | Full Text: HTML PDF (266K)
Supplement 2, February 1998
Unit 4.7 Transient Expression of Proteins Using COS Cells
Abstract | Full Text: HTML PDF (132K)
Unit 5.9 Overview of Membrane Protein Solubilization
Abstract | Full Text: HTML PDF (102K)
Unit 6.7 Patch-Pipet Recording in Brain Slices
Abstract | Full Text: HTML PDF (143K)
Unit 6.8 Single-Channel Recording
Abstract | Full Text: HTML PDF (362K)
Unit 7.7 Saturation Analysis of Ligand Binding Using a Centrifugation Procedure
Abstract | Full Text: HTML PDF (96K)
Unit 7.8 Ligand Characterization Using Microphysiometry
Abstract | Full Text: HTML PDF (385K)
Unit 7.9 Uptake and Release of Neurotransmitters
Abstract | Full Text: HTML PDF (241K)
Appendix 3B Techniques for Mammalian Cell Tissue Culture
Full Text: HTML PDF (159K)
Supplement 1, November 1997
Unit 3.9 Quantitative Analysis of In Vivo Cell Proliferation
Full Text: HTML PDF (348K)
Unit 3.10 Experimental Transplantation in the Embryonic, Neonatal, and Adult Mammalian Brain
HTML PDF (1013K)
Unit 7.6 Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites
Abstract | Full Text: HTML PDF (233K)
Unit 8.6 Overview of Methodological Approaches to the Study of Ingestive Behavior
Unit 8.7 Basic Measures of Food Intake
Appendix 1B Optimization of Transfection
Abstract | Full Text: HTML PDF (83K)
Appendix 1C Calcium Phosphate Transfection
Abstract | Full Text: HTML PDF (121K)
Appendix 1D Transfection Using DEAE -Dextran
Abstract | Full Text: HTML PDF (135K)
Appendix 1E Transfection by Electroporation
Abstract | Full Text: HTML PDF (89K)
Appendix 3A Preparation of an Iodinated Radioligand
Abstract | Full Text: HTML PDF (104K)
Core Publication, September 1997
Unit 1.1 Basic Neuroanatomical Methods
Abstract | Full Text: HTML PDF (116K)
Unit 1.2 Immunohistochemical Localization of Neurochemicals
Unit 1.3 Hybridization Histochemistry of Neural Transcripts
Unit 1.4 Receptor Binding Techniques
Abstract | Full Text: HTML PDF (205K)
Unit 2.1 Theory and Application of Fluorescence Microscopy
Full Text: HTML PDF (347K)
Unit 2.2 Basic Confocal Microscopy
Abstract | Full Text: HTML PDF (436K)
Unit 2.3 Imaging Nervous System Activity
Abstract | Full Text: HTML PDF (157K)
Unit 3.1 Culture of Neuroepithelial Stem Cells
Abstract | Full Text: HTML PDF (90K)
Unit 3.2 Long-Term Culture of Hippocampal Neurons
Unit 3.3 Culture of Substantia Nigra Neurons
Abstract | Full Text: HTML PDF (296K)
Unit 3.4 Isolation and Purification of Primary Oligodendrocyte Precursors
Abstract | Full Text: HTML PDF (125K)
Unit 3.5 Isolation and Purification of Primary Rodent Astrocytes
Abstract | Full Text: HTML PDF (181K)
Unit 3.6 Differentiation of Embryonic Stem Cells
HTML PDF (142K)
Unit 3.7 Immortalizing Central Nervous System Cells
Abstract | Full Text: HTML PDF (184K)
Unit 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR
Abstract | Full Text: HTML PDF (888K)
Unit 4.1 Homology Cloning of cDNA or Genomic DNA
Abstract | Full Text: HTML PDF (97K)
Unit 4.2 PCR Cloning of Neural Gene Products
Unit 4.3 Expression Cloning of Neural Genes Using Xenopus laevis Oocytes
Abstract | Full Text: HTML PDF (248K)
Unit 4.4 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Abstract | Full Text: HTML PDF (369K)
Unit 4.5 Overview of Neural Gene Expression
Abstract | Full Text: HTML PDF (99K)
Unit 4.6 Selection of Transfected Mammalian Cells
Abstract | Full Text: HTML PDF (244K)
Unit 5.1 RNA Analysis by Nuclease Protection
Unit 5.2 Differential Display of mRNA by PCR
Abstract | Full Text: HTML PDF (268K)
Unit 5.3 Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System
Abstract | Full Text: HTML PDF (258K)
Unit 5.4 Reducing Gene Expression in the Brain via Antisense Methods
Abstract | Full Text: HTML PDF (176K)
Unit 5.5 Production of Polyclonal Antisera
Abstract | Full Text: HTML PDF (140K)
Unit 5.6 Production of Antipeptide Antisera
Abstract | Full Text: HTML PDF (227K)
Unit 5.7 Production of Antisera Using Fusion Proteins
HTML PDF (186K)
Unit 5.8 Epitope Tagging of Recombinant Proteins
Abstract | Full Text: HTML PDF (161K)
Unit 6.1 The Electrophysiology Setup
Abstract | Full Text: HTML PDF (109K)
Unit 6.2 Principles of Signal Conditioning
Abstract | Full Text: HTML PDF (203K)
Unit 6.3 Fabrication of Patch Pipets
Unit 6.4 Preparation of Hippocampal Brain Slices
Abstract | Full Text: HTML PDF (145K)
Unit 6.5 Acute Isolation of Neurons from the Mature Mammalian Central Nervous System
Abstract | Full Text: HTML PDF (235K)
Unit 6.6 Whole-Cell Voltage Clamp Recording
Abstract | Full Text: HTML PDF (343K)
Unit 7.1 Overview of Microdialysis
HTML PDF (315K)
Unit 7.2 Microdialysis in Rodents
HTML PDF (293K)
Unit 7.3 Microdialysis in Nonhuman Primates
Abstract | Full Text: HTML PDF (461K)
Unit 7.4 Detection and Quantification of Neurotransmitters in Dialysates
Abstract | Full Text: HTML PDF (214K)
Unit 7.5 Analyzing Radioligand Binding Data
Full Text: HTML PDF (461K)
Unit 8.1 Locomotor Behavior
HTML PDF (133K)
Unit 8.2 Sexual and Reproductive Behaviors
Abstract | Full Text: HTML PDF (247K)
Unit 8.3 Animal Tests of Anxiety
Unit 8.4 Application of Experimental Stressors in Laboratory Rodents
HTML PDF (166K)
Unit 8.5 Assessment of Spatial Memory Using the Radial Arm and Morris Water Mazes
Appendix 1A Molecular Biology References
Abstract | Full Text: HTML PDF (67K)
Appendix 2A Common Stock Solutions, Buffers, and Media
Abstract | Full Text: HTML PDF (107K)
Appendix 2B Standard Laboratory Equipment
Abstract | Full Text: HTML PDF (62K)
Selected Suppliers of Reagents and Equipment
Abstract | Full Text: HTML PDF (250K)