Current Protocols in Neuroscience
Online ISBN: 9780471142300
Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.
Current Protocols in Neuroscience is the comprehensive resource for researchers investigating the nervous system. Researchers at all levels of expertise will find sufficient detail to permit duplication of protocols. Protocols include such critical topics as neuroanatomical and imaging methods; analysis of cell proliferation, cell damage and programmed cell death; molecular methods ranging from transfection of cells to phage display and gene delivery using a variety of vectors; isolation, culture, and sorting of specific neuronal and glial cell populations; tissue slices; electrophysiology setup and recordings, including exquisite detail on patch clamping and voltage clamping techniques, along with examples of synaptic current and potential recordings; neurochemistry and pharmacology; and a wide array of animal models for behavior and for neurologic and psychiatric disorders. This compilation brings together hundreds of core and cutting edge techniques reflecting the breadth of the field of neuroscience itself, making it a valuable asset for anyone pursuing a multidisciplinary approach.
The target audience is both the novice and the experienced experimenter—the first-year graduate student or new post-doctoral fellow and the seasoned investigator who wants to branch out into a new area of research. Sufficient detail is provided to permit duplication of the protocols in any laboratory, whatever the disciplinary background or level of sophis¬tication.
neuroscientists investigating areas including physiology, cell biology, neuroanatomy, molecular biology, developmental biology, pharmacology, behavior, neurological disease.
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