Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

Online ISBN: 9780471142706

DOI: 10.1002/0471142700

Mission Statement

Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.

Current Protocols in Nucleic Acid Chemistry is the comprehensive resource for detailed protocols related to nucleosides, nucleotides, and nucleic acids. CPNC brings together hundreds of core and cutting edge techniques for synthesis and analysis. Annotated steps provide a level of bench experience that is not found in the primary literature, vastly improving the ease with which researchers can duplicate the methods. Synthesis protocols include characterization data needed to verify that the desired products are obtained. Topics range from preparation of modified and unmodified nucleosides and their phosphoramidites, nucleoside protection strategies, nucleoside phosphorylation, standard oligonucleotide synthesis using phosphoramidite or H-phosphonate building blocks, large-scale synthesis methods, synthesis of conjugated oligonucleotides, siRNA synthesis, design and preparation of biologically active nucleosides and prodrugs, nucleic acid structure, nucleic acid binding molecules, nanotechnology, and combinatorial libraries.

The target audience for Current Protocols in Nucleic Acid Chemistry is anyone who is interested in applying bioinformatic techniques to their own research questions, and no prior experience in the field is assumed. Protocols are written in sufficient detail so that readers can easily follow and perform the analyses described in Current Protocols in Nucleic Acid Chemistry on their own, making it the ideal companion for experimentalists in all biological disciplines.


chemists and biologists studying nucleosides, nucleotides, oligonucleotides and nucleic acids, including their design, synthesis, analysis, and therapeutic application


DNA, RNA, nucleoside, nucleotide, oligonucleotide, nucleic acid, protecting group, chemistry, analytical chemistry, synthetic chemistry, biophysics, structure, laboratory protocols, methods, Current Protocols