Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

About this Book

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What's New in Current Protocols in Molecular Biology
Supplement 106, April 2014

Unit 1.16 Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Abstract | Full Text: HTML PDF

Unit 4.21 Next-Generation Sequencing RNA-Seq Library Construction
Abstract | Full Text: HTML PDF

Unit 7.16 Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms
Abstract | Full Text: HTML PDF

Unit 28.4 Detection of Mycoplasma Contamination in Cell Cultures
Abstract | Full Text: HTML PDF

Unit 28.5 Eradication of Mycoplasma Contaminations from Cell Cultures
Abstract | Full Text: HTML PDF

An essential tool for anyone at the forefront of molecular biology research, Current Protocols in Molecular Biology—the first Current Protocols title—remains the benchmark by which all other protocol resources are judged. With an extensive range of information, from basic methods to advanced procedures, Current Protocols in Molecular Biology provides incomparable coverage of this ever-expanding field.

A subscription gives you access to all the content in the collection plus four quarterly issues of new and updated content. Current Protocols in Molecular Biology...

  • covers basic methods, such as nucleic acid isolation, purification, and quantition
  • contains updated information and protocols on rapidly changing areas such as Next-Generation Sequencing, RNAi, and zincfinger nucleases
  • offers advanced procedures for microarray analysis, chromatin assembly and analysis, single-cell analysis and gene silencing, among others
  • explores specialized areas, such as metabolomics

Edited by: Fred M. Ausubel (Massachusetts General Hospital and Harvard Medical School); Roger Brent (Fred Hutchinson Cancer Research Center); Robert E. Kingston (Massachusetts General Hospital and Harvard Medical School); David D. Moore (Baylor College of Medicine); J.G. Seidman (Harvard Medical School); John A. Smith (University of Alabama at Birmingham); Kevin Struhl (Harvard Medical School). Guest Editors: Lisa M. Albright (Austin, Texas); Donald M. Coen (Harvard Medical School); Andrew F. Gardner (New England Biolabs); Ruslan I. Sadreyev (Massachusetts General Hospital and Harvard Medical School); Barton E. Slatko (New England Biolabs); Carolyn L. Smith (Baylor College of Medicine); Ajit Varki (University of California San Diego); Mary C. Wildermuth (University of California Berkeley). Past Guest Editors: Mark L. Borowsky (Massachusetts General Hospital); Reuben Shaw (The Salk Institute for Biological Studies)

Developmental Editor: Gwen P. Taylor

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.

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