Current Protocols in Molecular Biology
Online ISBN: 9780471142720
DOI: 10.1002/0471142727
Archive
Current Protocols in Molecular Biology Supplements
2013: Supplement 102 Supplement 101
2012: Supplement 100 Supplement 99 Supplement 98 Supplement 97
2011: Supplement 96 Supplement 95 Supplement 94 Supplement 93
2010: Supplement 92 Supplement 91 Supplement 90 Supplement 89
2009: Supplement 88 Supplement 87 Supplement 86 Supplement 85
2008: Supplement 84 Supplement 83 Supplement 82 Supplement 81
2007: Supplement 80 Supplement 79 Supplement 78 Supplement 77
2006: Supplement 76 Supplement 75 Supplement 74 Supplement 73
2005: Supplement 72 Supplement 71 Supplement 70 Supplement 69
2004: Supplement 68 Supplement 67 Supplement 66 Supplement 65
2003: Supplement 64 Supplement 63 Supplement 62 Supplement 61
2002: Supplement 60 Supplement 59 Supplement 58 Supplement 57
2001: Supplement 56 Supplement 55 Supplement 54 Supplement 53
2000: Supplement 52 Supplement 51 Supplement 50 Supplement 49
1999: Supplement 48 Supplement 47 Supplement 46 Supplement 45
1998: Supplement 44 Supplement 43 Supplement 42 Supplement 41
1997: Supplement 40 Supplement 39 Supplement 38 Supplement 37
1996: Supplement 36 Supplement 35 Supplement 34 Supplement 33
1995: Supplement 32 Supplement 31 Supplement 30 Supplement 29
1994: Supplement 28 Supplement 27 Supplement 26 Supplement 25
1993: Supplement 24 Supplement 23 Supplement 22 Supplement 21
1992: Supplement 20 Supplement 19 Supplement 18 Supplement 17
1991: Supplement 16 Supplement 15 Supplement 14 Supplement 13
1990: Supplement 12 Supplement 11 Supplement 10 Supplement 9
1989: Supplement 8 Supplement 7 Supplement 6 Supplement 5
1988: Supplement 4 Supplement 3 Supplement 2 Supplement 1
1987: Core publication
Supplement 102, April 2013
Unit 2.14 DNA Isolation from Mammalian Samples
Abstract | Full Text: HTML PDF
Unit 4.15 Synthesis and Labeling of RNA In Vitro
Abstract | Full Text: HTML PDF
Unit 7.12 Exome Sequencing by Targeted Enrichment
Abstract | Full Text: HTML PDF
Unit 14.15 Basic Image Analysis and Manipulation in ImageJ
Abstract | Full Text: HTML PDF
Unit 21.26 A Detailed Protocol for Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)
Abstract | Full Text: HTML PDF
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Supplement 101, January 2013
Unit 7.11 Multiplex Illumina Sequencing Using DNA Barcoding
Abstract | Full Text: HTML PDF
Unit 14.22 Using CellX to Quantify Intracellular Events
Abstract | Full Text: HTML PDF
Unit 18.20 Using Phospho-Motif Antibodies to Determine Kinase Substrates
Abstract | Full Text: HTML PDF
Unit 19.1 Detecting the Signatures of Adaptive Evolution in Protein-Coding Genes
Abstract | Full Text: HTML PDF
Unit 21.25 Capture Hybridization Analysis of RNA Targets (CHART)
Abstract | Full Text: HTML PDF
Unit 22.1 Overview of DNA Microarrays: Types, Applications, and Their Future
Abstract | Full Text: HTML PDF
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Supplement 100, October 2012
Unit 12.15 Engineering Designer Transcription Activator–Like Effector Nucleases (TALENs) by REAL or REAL-Fast Assembly
Abstract | Full Text: HTML PDF
Unit 14.18 Using Cell-ID 1.4 with R for Microscope-Based Cytometry
Abstract | Full Text: HTML PDF
Unit 21.24 ChIP-exo Method for Identifying Genomic Location of DNA-Binding Proteins with Near-Single-Nucleotide Accuracy
Abstract | Full Text: HTML PDF
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Supplement 99, July 2012
Unit 3.23 Design and Assembly of Large Synthetic DNA Constructs
Abstract | Full Text: HTML PDF
Unit 3.24 Enrichment of Error-Free Synthetic DNA Sequences by CEL I Nuclease
Abstract | Full Text: HTML PDF
Unit 3.25 Database of DNA Polymerases
Abstract | Full Text: HTML PDF
Unit 10.29 Biochemical Analysis of Protein SUMOylation
Abstract | Full Text: HTML PDF
Unit 10.30 Assays for Investigating deSUMOylation Enzymes
Abstract | Full Text: HTML PDF
Unit 21.23 Single Nucleotide Analysis of Cytosine Methylation by Whole-Genome Shotgun Bisulfite Sequencing
Abstract | Full Text: HTML PDF
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Supplement 98, April 2012
Unit 2.13 Preparation of Genomic DNA from Mammalian Sperm
Abstract | Full Text: HTML PDF
Unit 4.13 RASL-seq for Massively Parallel and Quantitative Analysis of Gene Expression
Abstract | Full Text: HTML PDF
Unit 4.14 Native Elongating Transcript Sequencing (NET-seq)
Abstract | Full Text: HTML PDF
Unit 14.21 Use of YouScope to Implement Systematic Microscopy Protocols
Abstract | Full Text: HTML PDF
Unit 18.19 Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
Abstract | Full Text: HTML PDF
Unit 30.2 Targeted Metabolomics
Abstract | Full Text: HTML PDF
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Supplement 97, January 2012
Unit 10.2A One-Dimensional SDS Gel Electrophoresis of Proteins
Abstract | Full Text: HTML PDF
Unit 12.14 In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
Abstract | Full Text: HTML PDF
Unit 19.4 Protein Databases on the Internet
Abstract | Full Text: HTML PDF
Unit 23.15 Reprogramming of Mouse, Rat, Pig, and Human Fibroblasts into iPS Cells
Abstract | Full Text: HTML PDF
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Supplement 96, October 2011
Unit 3.11 Exonucleases
Abstract | Full Text: HTML PDF
Unit 7.0 Introduction and Historical Overview of DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 7.1 Overview of DNA Sequencing Strategies
Abstract | Full Text: HTML PDF
Unit 7.2 “First Generation” Automated DNA Sequencing Technology
Abstract | Full Text: HTML PDF
Unit 7.5 High-Throughput Sequencing of PCR Products Tagged with Universal Primers Using 454 Life Sciences System
Abstract | Full Text: HTML PDF
Unit 12.13 Engineering Designer Nucleases with Customized Cleavage Specificities
Abstract | Full Text: HTML PDF
Unit 19.11 Phylogenetic Analysis of Protein Sequence Data Using the Randomized Axelerated Maximum Likelihood (RAXML) Program
Abstract | Full Text: HTML PDF
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Supplement 95, July 2011
Unit 15.10 Stem-Loop RT-qPCR for miRNAs
Abstract | Full Text: HTML PDF
Unit 17.6 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
Abstract | Full Text: HTML PDF
Unit 21.22 Simultaneous Single-Molecule Mapping of Protein-DNA Interactions and DNA Methylation by MAPit
Abstract | Full Text: HTML PDF
Unit 23.14 Recombineering-Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
Abstract | Full Text: HTML PDF
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Supplement 94, April 2011
Unit 3.14 DNA Ligases
Abstract | Full Text: HTML PDF
Unit 3.22 Gene and Genome Construction in Yeast
Abstract | Full Text: HTML PDF
Unit 4.12 Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease
Abstract | Full Text: HTML PDF
Unit 10.28 The Combined Use of the Thermofluor Assay and ThermoQ Analytical Software for the Determination of Protein Stability and Buffer Optimization as an Aid in Protein Crystallization
Abstract | Full Text: HTML PDF
Unit 21.21 DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions In Vivo
Abstract | Full Text: HTML PDF
Unit 25B.10 Digital Multiplexed Gene Expression Analysis Using the NanoString nCounter System
Abstract | Full Text: HTML PDF
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Supplement 93, January 2011
Unit 3.12 Endonucleases
Abstract | Full Text: HTML PDF
Unit 13.10B Conditional Depletion of Nuclear Proteins by the Anchor Away System
Abstract | Full Text: HTML PDF
Unit 25B.9 Quantification of Gene Transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) Using 1 to 2 μg Total RNA
Abstract | Full Text: HTML PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract | Full Text: HTML PDF
Appendix 3J Microvolume Quantitation of Nucleic Acids
Abstract | Full Text: HTML PDF
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Supplement 92, October 2010
Unit 7.10 Single Molecule Sequencing with a HeliScope Genetic Analysis System
Abstract | Full Text: HTML PDF
Unit 9.3 Transfection of Electroporation
Abstract | Full Text: HTML PDF
Unit 13.2B Drug-Induced Permeabilization of S. cerevisiae
Abstract | Full Text: HTML PDF
Unit 14.20 Computer Control of Microscopes Using μManager
Abstract | Full Text: HTML PDF
Unit 18.18 Serine/Threonine Protein Phosphatase Assays
Abstract | Full Text: HTML PDF
Unit 21.20 Genome-Wide Location Analysis by Pull Down of In Vivo Biotinylated Transcription Factors
Abstract | Full Text: HTML PDF
Appendix 6 Pathway Modulators and Inhibitors
Abstract | Full Text: HTML PDF
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Supplement 91, July 2010
Unit 7.9 Bisulfite Sequencing of DNA
Abstract | Full Text: HTML PDF
Unit 18.14 Analysis of Serine-Threonine Kinase Specificity Using Arrayed Positional Scanning Peptide Libraries
Abstract | Full Text: HTML PDF
Unit 18.15 Visualization of Kinase Activity with FRET-Based Activity Biosensors
Abstract | Full Text: HTML PDF
Unit 18.16 Analysis of Protein Tyrosine Phosphatases and Substrates
Abstract | Full Text: HTML PDF
Unit 18.17 Fluorescent Peptide Assays for Protein Kinases
Abstract | Full Text: HTML PDF
Unit 21.19 ChIP-Seq: A Method for Global Identification of Regulatory Elements in the Genome
Abstract | Full Text: HTML PDF
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Supplement 90, April 2010
Unit 5.12 Normalizing cDNA Libraries
Abstract | Full Text: HTML PDF
Unit 10.26 Isolation of Proteins by Heat-Induced Extraction from Formalin-Fixed, Paraffin-Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis
Abstract | Full Text: HTML PDF
Unit 10.27 Extraction of Proteins from Formalin-Fixed, Paraffin-Embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/Mass Spectrometry Analysis
Abstract | Full Text: HTML PDF
Unit 21.18 Installation of Site-Specific Methylation into Histones Using Methyl Lysine Analogs
Abstract | Full Text: HTML PDF
Unit 30.1 Untargeted Metabolomics
Abstract | Full Text: HTML PDF
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Supplement 89, January 2010
Unit 4.11 RNA-Seq: A Method for Comprehensive Transcriptome Analysis
Abstract | Full Text: HTML PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release from N-Linked Glycans
Abstract | Full Text: HTML PDF
Unit 19.10 Galaxy: A Web-Based Genome Analysis Tool for Experimentalists
Abstract | Full Text: HTML PDF
Unit 21.15 Chromatin Interaction Analysis Using Paired-End Tag Sequence
Abstract | Full Text: HTML PDF
Unit 21.16 Mapping Networks of Protein-Mediated Physical Interactions Between Chromatin Elements
Abstract | Full Text: HTML PDF
Unit 21.17 Methylation-Sensitive Single-Molecule Analysis of Chromatin Structure
Abstract | Full Text: HTML PDF
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Supplement 88, October 2009
Unit 10.25 Preparation of Proteins and Peptides for Mass Spectrometry Analysis in a Bottom-Up Proteomics Workflow
Abstract | Full Text: HTML PDF
Unit 15.9 Heat-Activatable Primers for Hot-Start PCR and Hot-Start One-Step RT-PCR: Endpoint and Real-Time Experiments
Abstract | Full Text: HTML PDF
Unit 19.9 The UCSC Genome Browser: What Every Molecular Biologist Should Know
Abstract | Full Text: HTML PDF
Unit 24.2 Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
Abstract | Full Text: HTML PDF
Unit 24.3 In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
Abstract | Full Text: HTML PDF
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Supplement 87, July 2009
Unit 17.4 Metabolic Radiolabeling of Animal Cell Glycoconjugates
Abstract | Full Text: HTML PDF
Unit 19.8 Resources for Small Regulatory RNAs
Abstract | Full Text: HTML PDF
Unit 24.6 Directed Evolution of Proteins In Vitro Using Compartmentalization in Emulsions
Abstract | Full Text: HTML PDF
Unit 25A.3 Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA
Abstract | Full Text: HTML PDF
Appendix 6 Pathway Modulators and Inhibitors
Abstract | Full Text: HTML PDF
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Supplement 86, April 2009
Unit 3.21 DNA Cloning and Engineering by Uracil Excision
Abstract | Full Text: HTML PDF
Unit 9.5 Selection of Transfected Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 19.7 Using PATIMDB to Create Bacterial Transposon Insertion Mutant Libraries
Abstract | Full Text: HTML PDF
Unit 20.7 Two-Hybrid Dual Bait System
Abstract | Full Text: HTML PDF
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Supplement 85, January 2009
Unit 10.6 Staining Proteins in Gels
Abstract | Full Text: HTML PDF
Unit 22.4 Overview of mRNA Expression Profiling Using DNA Microarrays
Abstract | Full Text: HTML PDF
Unit 22.5 Pattern Discovery in Expression Profiling Data
Abstract | Full Text: HTML PDF
Unit 23.13 Recombineering-Based Procedure for Creating Cre/loxP Conditional Knockouts in the Mouse
Abstract | Full Text: HTML PDF
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Supplement 84, October 2008
Unit 3.5 DNA-Dependent DNA Polymerases
Abstract | Full Text: HTML PDF
Unit 3.6 Template-Independent DNA Polymerases
Abstract | Full Text: HTML PDF
Unit 3.7 RNA-Dependent DNA Polymerases
Abstract | Full Text: HTML PDF
Unit 3.8 RNA Polymerases
Abstract | Full Text: HTML PDF
Unit 3.9 DNA Repair Enzymes
Abstract | Full Text: HTML PDF
Unit 3.13 Ribonucleases
Abstract | Full Text: HTML PDF
Unit 3.15 RNA Ligases
Abstract | Full Text: HTML PDF
Unit 10.7 Detection of Proteins on Blot Transfer Membranes
Abstract | Full Text: HTML PDF
Unit 14.18 Using Cell-ID 1.4 with R for Microscope-Based Cytometry
Abstract | Full Text: HTML PDF
Unit 14.19 Visualization of Microscopy-Based Spectral Imaging Data from Multi-Label Tissue Sections
Abstract | Full Text: HTML PDF
Unit 29B.5 Dietary Manipulation of Mouse Metabolism
Abstract | Full Text: HTML PDF
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Supplement 83, July 2008
Unit 10.8 Immunoblotting and Immunodetection
Abstract | Full Text: HTML PDF
Unit 11.10 Production of Monoclonal Antibody Supernatant and Ascites Fluid
Abstract | Full Text: HTML PDF
Unit 26.8 Using Morpholinos to Control Gene Expression
Abstract | Full Text: HTML PDF
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Supplement 82, April 2008
Unit 13.2 Growth and Manipulation of Yeast
Abstract | Full Text: HTML PDF
Unit 13.3B EMS and UV Mutagenesis in Yeast
Abstract | Full Text: HTML PDF
Unit 14.8 In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets
Abstract | Full Text: HTML PDF
Unit 14.17 Using CellProfiler for Automatic Identification and Measurement of Biological Objects in Images
Abstract | Full Text: HTML PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Abstract | Full Text: HTML PDF
Unit 23.5 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
Abstract | Full Text: HTML PDF
Unit 23.6 Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout)
Abstract | Full Text: HTML PDF
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Supplement 81, January 2008
Unit 7.1 Overview of DNA Sequencing Strategies
Abstract | Full Text: HTML PDF
Unit 9.4 Tranfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
Abstract | Full Text: HTML PDF
Unit 11.12 Production of Polyclonal Antisera
Abstract | Full Text: HTML PDF
Unit 14.6 Immunohistochemistry
Abstract | Full Text: HTML PDF
Unit 14.11 Basic Confocal Microscopy
Abstract | Full Text: HTML PDF
Unit 26.7 Analysis of Small Endogenous RNAs
Abstract | Full Text: HTML PDF
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Supplement 80, October 2007
Unit 21.14 Chromosome Conformation Capture Carbon Copy Technology
Abstract | Full Text: HTML PDF
Unit 24.1 Overview of Receptors from Combinatorial Nucleic Acid and Protein Libraries
Abstract | Full Text: HTML PDF
Unit 25B.6 Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
Abstract | Full Text: HTML PDF
Unit 29A.4 Tissue Collection for Systematic Phenotyping in the Mouse
Abstract | Full Text: HTML PDF
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Supplement 79, July 2007
Unit 1.17 E. coli Genome Manipulation by P1 Transduction
Abstract | Full Text: HTML PDF
Unit 14.7 In Situ Hybridization and Detection Using Nonisotopic Probes
Abstract | Full Text: HTML PDF
Unit 21.12 Paired-End diTagging for Transcriptome and Genome Analysis
Abstract | Full Text: HTML PDF
Unit 21.13 ChIP-chip for Genome-Wide Analysis of Protein Binding in Mammalian Cells
Abstract | Full Text: HTML PDF
Appendix 1E Radioactivity
Abstract | Full Text: HTML PDF
Appendix 1F Safe Use of Radioisotopes
Abstract | Full Text: HTML PDF
Supplement 78, April 2007
Unit 1.16 Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Abstract | Full Text: HTML PDF
Unit 3.17 Constructing Recombinant DNA Molecules by PCR
Abstract | Full Text: HTML PDF
Unit 4.10 Identification of Newly Transcribed RNA
Abstract | Full Text: HTML PDF
Unit 16.25 Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
Abstract | Full Text: HTML PDF
Unit 29B.4 Histopathology in Mouse Metabolic Investigations
Abstract | Full Text: HTML PDF
Supplement 77, January 2007
Unit 18.13 Isolation of Phosphopeptides by Immobilized Metal Ion Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 19.6 Analysis and Management of Microarray Gene Expression Data
Abstract | Full Text: HTML PDF
Unit 29B.3 Evaluation of Glucose Homeostasis
Abstract | Full Text: HTML PDF
Supplement 76, October 2006
Unit 7.8 Polony DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 10.1A Spectrophotometric and Colorimetric Determination of Protein Concentration
Abstract | Full Text: HTML PDF
Unit 20.5 Detection of Protein-Protein Interactions by Coprecipitation
Abstract | Full Text: HTML PDF
Unit 23.1 Overview of Gene Targeting by Homologous Recombination
Abstract | Full Text: HTML PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract | Full Text: HTML PDF
Supplement 75, July 2006
Unit 10.2A One-Dimensional SDS Gel Electrophoresis of Proteins
Abstract | Full Text: HTML PDF
Unit 12.1 Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 21.12 Paired-End diTagging for Transcriptome and Genome Analysis
Full Text: HTML PDF
Unit 27.4 RNA Immunoprecipitation for Determining RNA-Protein Associations In Vivo
Abstract | Full Text: HTML PDF
Unit 29A.3 Collection of Blood and Plasma from the Mouse
Abstract | Full Text: HTML PDF
Unit 29B.2 Lipid and Bile Acid Analysis
Abstract | Full Text: HTML PDF
Supplement 74, April 2006
Unit 3.20 Recombinational Cloning
Abstract | Full Text: HTML PDF
Unit 10.24 Solution Radioimmunoassay of Proteins and Peptides
Abstract | Full Text: HTML PDF
Unit 14.16 Three-Dimensional Reconstruction of Tissues
Abstract | Full Text: HTML PDF
Unit 21.11 Mapping Chromatin Interactions by Chromosome Conformation Capture
Abstract | Full Text: HTML PDF
Appendix 3F Techniques for Mammalian Cell Tissue Culture
Abstract | Full Text: HTML PDF
Supplement 73, January 2006
Unit 15.8 High-Throughput Real-Time Quantitative Reverse Transcription PCR
Abstract | Full Text: HTML PDF
Unit 18.12 The Detection of MAPK Signaling
Abstract | Full Text: HTML PDF
Unit 29A.1 Uses of Forward and Reverse Genetics in Mice to Study Gene Function
Abstract | Full Text: HTML PDF
Unit 29A.2 Minimizing Variation Due to Genotype and Environment
Abstract | Full Text: HTML PDF
Unit 29B.1 Evaluation of Energy Homeostasis
Abstract | Full Text: HTML PDF
Supplement 72, October 2005
Unit 10.24 Solution Radioimmunoassay of Proteins and Peptides
Full Text: HTML PDF
Unit 21.10 Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE)
Abstract | Full Text: HTML PDF
Unit 26.4 Cloning of Small RNA Molecules
Abstract | Full Text: HTML PDF
Unit 26.6 RNAi in Transgenic Plants
Abstract | Full Text: HTML PDF
Supplement 71, July 2005
Unit 15.8 High-Throughput Real-Time Quantitative Reverse Transcription PCR
Full Text: HTML PDF
Unit 23.10 Transgenic Mouse Colony Management
Abstract | Full Text: HTML PDF
Unit 23.11 Modification and Production of BAC Transgenes
Abstract | Full Text: HTML PDF
Unit 23.12 Regulation of Transgene Expression Using Tetracycline
Abstract | Full Text: HTML PDF
Unit 28.3 Establishment and Culture of Human Skin Fibroblasts
Abstract | Full Text: HTML PDF
Supplement 70, April 2005
Unit 1.16 Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Full Text: HTML PDF
Unit 19.5 Basic Protein Sequence Analysis
Abstract | Full Text: HTML PDF
Unit 21.8 Analysis of Protein Co-Occupancy by Quantitative Sequential Chromatin Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 28.1 Preparation, Culture, and Immortalization of Mouse Embryonic Fibroblasts
Abstract | Full Text: HTML PDF
Unit 28.2 Isolation and Immortalization of Lymphocytes
Abstract | Full Text: HTML PDF
Appendix 1A Common Abbreviations
Abstract |Full Text: HTML PDF
Supplement 69, January 2005
Unit 10.23 Difference Gel Electrophoresis (DIGE) Using CyDye DIGE Fluor Minimal Dyes
Abstract | Full Text: HTML PDF
Unit 16.24 Helper-Dependent Adenoviral Vectors
Abstract | Full Text: HTML PDF
Unit 21.1 Micrococcal Nuclease Analysis of Chromatin Structure
Abstract | Full Text: HTML PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
Abstract | Full Text: HTML PDF
Unit 22.5 Pattern Discovery in Expression Profiling Data
Full Text: HTML PDF
Supplement 68, October 2004
Unit 2.8 Capillary Electrophoresis of DNA
Abstract | Full Text: HTML PDF
Unit 19.4 Protein Databases on the Internet
Abstract | Full Text: HTML PDF
Unit 21.8 Analysis of Protein Co-Occupancy by Quantitative Sequential Chromatin Immunoprecipitation
Full Text: HTML PDF
Unit 21.9 Defining In Vivo Targets of Nuclear Proteins by Chromatin Immunoprecipitation and Microarray Analysis
Abstract | Full Text: HTML PDF
Unit 23.9 Transgenic Mouse Production By Zygote Injection
Abstract | Full Text: HTML PDF
Supplement 67, July 2004
Unit 4.9 Analysis of RNA by Northern and Slot Blot Hybridization
Abstract | Full Text: HTML PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 18.12 The Detection of MAPK Signaling
Full Text: HTML PDF
Unit 22.4 Overview of mRNA Expression Profiling Using Microarrays
Full Text: HTML PDF
Supplement 66, April 2004
Unit 10.5 Overview of Digital Electrophoresis Analysis
Abstract | Full Text: HTML PDF
Unit 10.8 Immunoblotting and Immunodetection
Full Text: HTML PDF
Unit 14.9 Whole-Mount In Situ Hybridization and Detection of RNA s in Vertebrate Embryos and Isolated Organs
Abstract | Full Text: HTML PDF
Unit 18.11 Design and Use of Analog-Sensitive Protein Kinases
Abstract | Full Text: HTML PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Full Text: HTML PDF
Supplement 65, January 2004
Unit 16.9 Overview of the Baculovirus Expression System
Abstract | Full Text: HTML PDF
Unit 16.10 Maintenance of Insect Cell Cultures and Generation of Recombinant Baculoviruses
Abstract | Full Text: HTML PDF
Unit 16.11 Expression and Purification of Recombinant Proteins Using the Baculovirus System
Abstract | Full Text: HTML PDF
Unit 20.8 Interaction Trap/Two-Hybrid System to Identify Loss-of-Interaction Mutant Proteins
Abstract | Full Text: HTML PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
Full Text: HTML PDF
Unit 26.5 RNA Interference in Cultured Drosophila Cells
Abstract | Full Text: HTML PDF
Supplement 64, October 2003
Unit 10.7 Detection of Proteins on Blot Transfer Membranes
Full Text: HTML PDF
Unit 13.14 Overview of Schizosaccharomyces pombe
Abstract | Full Text: HTML PDF
Unit 13.15 S. pombe Strain Maintenance and Media
Abstract | Full Text: HTML PDF
Unit 13.16 Growth and Manipulation of S. pombe
Abstract | Full Text: HTML PDF
Unit 13.17 Introduction of DNA into S. pombe Cells
Abstract | Full Text: HTML PDF
Unit 26.4 Cloning of Small RNA Molecules
Full Text: HTML PDF
Appendix 5 Vectors
Abstract | Full Text: HTML PDF
Supplement 63, July 2003
Unit 9.1 Calcium Phosphate Transfection
Abstract | Full Text: HTML PDF
Unit 10.6 Staining Proteins in Gels
Full Text: HTML PDF
Unit 14.15 Overview of Image Analysis, Image Importing, and Image Processing Using Freeware
Abstract | Full Text: HTML PDF
Unit 27.1 Agarose Gel Separation/Isolation of RNA-Protein Complexes
Abstract | Full Text: HTML PDF
Unit 27.2 Identification of RNA Binding Proteins by UV Cross-Linking
Abstract | Full Text: HTML PDF
Unit 27.3 Purification of Functional RNA-Protein Complexes Using MS 2-MBP
Abstract | Full Text: HTML PDF
Supplement 62, April 2003
Unit 9.15 Human Somatic Cell Gene Targeting
Abstract | Full Text: HTML PDF
Unit 18.10 Use of Protein Phosphatase Inhibitors
Abstract | Full Text: HTML PDF
Unit 26.1 Overview of RNA Interference and Related Processes
Abstract | Full Text: HTML PDF
Unit 26.2 Gene Silencing by RNAi in Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 26.3 RNA Interference in Caenorhabditis Elegans
Abstract | Full Text: HTML PDF
Appendix 1A Common Abbreviations
Full Text: HTML PDF
Supplement 61, January 2003
Unit 1.16 Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Full Text: HTML PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
Unit 25B.6 Serial Analysis of Gene Expression (SAGE)
Full Text: HTML PDF
Unit 25B.8 Gene Expression Analysis of a Single or Few Cells
Abstract | Full Text: HTML PDF
Supplement 60, October 2002
Unit 16.12 Transient Expression of Proteins Using COS Cells
Abstract | Full Text: HTML PDF
Unit 16.13 Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 16.14 Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
Abstract | Full Text: HTML PDF
Unit 16.21 Overview of the HIV-1 Lentiviral Vector System
Abstract | Full Text: HTML PDF
Unit 16.22 Generation of HIV-1-Based Lentiviral Vector Particles
Abstract | Full Text: HTML PDF
Unit 16.23 Amplification Using CHO Cell Expression Vectors
Abstract | Full Text: HTML PDF
Unit 20.7 Two-Hybrid Dual Bait System
Full Text: HTML PDF
Unit 25B.7 Representational Difference Analysis
Abstract | Full Text: HTML PDF
Supplement 59, July 2002
Unit 1.1 Media Preparation and Bacteriological Tools
Abstract | Full Text: HTML PDF
Unit 1.2 Growth in Liquid Media
Abstract | Full Text: HTML PDF
Unit 1.3 Growth on Solid Media
Abstract | Full Text: HTML PDF
Unit 1.4 Selected Topics from Classical Bacterial Genetics
Abstract | Full Text: HTML PDF
Unit 2.1A Purification and Concentration of DNA from Aqueous Solutions
Abstract | Full Text: HTML PDF
Unit 2.6 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
Abstract | Full Text: HTML PDF
Unit 11.15 Introduction to Peptide Synthesis
Abstract | Full Text: HTML PDF
Unit 11.16 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
Abstract | Full Text: HTML PDF
Appendix 1K Compendium of Drugs Commonly Used in Molecular Biology Research
Abstract | Full Text: HTML PDF
Supplement 58, April 2002
Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture Cells
Abstract | Full Text: HTML PDF
Unit 21.7 Chromatin Assembly Using Drosophila Systems
Abstract | Full Text: HTML PDF
Unit 25A.2 Preparation of Single Cells from Solid Tissues for Analysis by PCR
Abstract | Full Text: HTML PDF
Appendix 1H Safe Use of Hazardous Chemicals
Abstract | Full Text: HTML PDF
Supplement 57, January 2002
Unit 17.6 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
Abstract | Full Text: HTML PDF
Unit 23.8 Mouse Colony Management
Abstract | Full Text: HTML PDF
Unit 25B.5 AFLP-Based Transcript Profiling
Abstract | Full Text: HTML PDF
Unit 25B.6 Serial Analysis of Gene Expression (SAGE)
Supplement 56, October 2001
Unit 2.4 Preparation of Genomic DNA from Bacteria
Abstract | Full Text: HTML PDF
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Abstract | Full Text: HTML PDF
Unit 15.2 Direct DNA Sequencing of PCR Products
Abstract | Full Text: HTML PDF
Unit 15.3 Ligation-Mediated PCR for Genomic Sequencing and Footprinting
Abstract | Full Text: HTML PDF
Unit 15.4 Molecular Cloning of PCR Products
Abstract | Full Text: HTML PDF
Unit 15.5 Enzymatic Amplification of RNA by PCR (RT-PCR)
Abstract | Full Text: HTML PDF
Unit 15.7 Quantitation of Rare DNAs by PCR
Abstract | Full Text: HTML PDF
Unit 25B.3 Differential Display of mRNA by PCR
Abstract | Full Text: HTML PDF
Unit 25B.4 Restriction-Mediated Differential Display (RMDD)
Abstract | Full Text: HTML PDF
Supplement 55, July 2001
Unit 5.9 Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
Abstract | Full Text: HTML PDF
Unit 12.12 Yeast One-Hybrid Screening for DNA-Protein Interactions
Abstract | Full Text: HTML PDF
Unit 14.3 In Situ Hybridization to Cellular RNA
Abstract | Full Text: HTML PDF
Unit 20.6 Identification of Protein Interactions by Far Western Analysis
Abstract | Full Text: HTML PDF
Unit 25A.1 Laser Capture Microdissection
Abstract | Full Text: HTML PDF
Unit 25B.1 Production of a Subtracted cDNA Library
Abstract | Full Text: HTML PDF
Unit 25B.2 PCR-Based Subtractive cDNA Cloning
Abstract | Full Text: HTML PDF
Supplement 54, April 2001
Unit 10.12 HPLC of Peptides and Proteins: Preparation and System Set-Up
Abstract | Full Text: HTML PDF
Unit 10.13 HPLC of Peptides and Proteins: Standard Operating Conditions
Abstract | Full Text: HTML PDF
Unit 10.14 Reversed-Phase Isolation of Peptides
Abstract | Full Text: HTML PDF
Unit 16.22 Generation of HIV-1-Based Lentiviral Vector Particles
Unit 21.6 Assembly of Nucleosomal Templates by Salt Dialysis
Abstract | Full Text: HTML PDF
Supplement 53, January 2001
Unit 11.18 Identification of Polyol-Responsive Monoclonal Antibodies for Use in Immunoaffinity Chromatography
Abstract | Full Text: HTML PDF
Unit 22.3 Profiling Human Gene Expression with cDNA Microarrays
Abstract | Full Text: HTML PDF
Unit 23.7 Chimeric Mouse Production by Microinjection
Abstract | Full Text: HTML PDF
Unit 24.5 Protein Selection Using mRNA Display
Abstract | Full Text: HTML PDF
Supplement 52, October 2000
Unit 23.4 Mouse Embryonic Stem (ES) Cell Isolation
Abstract | Full Text: HTML PDF
Unit 23.5 Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
Full Text: HTML PDF
Unit 23.6 Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout)
Full Text: HTML PDF
Unit 24.2 Design, Synthesis, and Amplification of DNA Pools for Construction of Combinatorial Pools and Libraries
Full Text: HTML PDF
Unit 24.3 In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
Full Text: HTML PDF
Unit 24.4 Peptide Aptamers: Dominant "Genetic" Agents for Forward and Reverse Analysis of Cellular Processes
Abstract | Full Text: HTML PDF
Supplement 51, July 2000
Unit 2.5A Agarose Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 2.5B Pulsed-Field Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 8.3 Random Mutagenesis by PCR
Abstract | Full Text: HTML PDF
Unit 10.6 Staining Proteins in Gels
Unit 13.3 Genome-Wide Transposon Mutagenesis in Yeast
Abstract | Full Text: HTML PDF
Unit 19.2 Sequence Databases: Integrated Information Retrieval and Data Submission
Abstract | Full Text: HTML PDF
Unit 23.1 Overview of Gene Targeting by Homologous Recombination
Full Text: HTML PDF
Unit 23.2 Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation
Abstract | Full Text: HTML PDF
Unit 23.3 Mouse Embryonic Stem (ES) Cell Culture
Abstract | Full Text: HTML PDF
Supplement 50, April 2000
Unit 11.13 In Vitro Antibody Production
Abstract | Full Text: HTML PDF
Unit 11.14 Purification of Immunoglobulin G Fraction from Antiserum, Ascites Fluid, or Hybridoma Supernatant
Abstract | Full Text: HTML PDF
Unit 11.17 Determination of the Specific Antibody Titer
Abstract | Full Text: HTML PDF
Unit 14.14 Whole-Mount Histochemical Detection of β-Galactosidase Activity
Abstract | Full Text: HTML PDF
Unit 19.1 Internet Basics for Biologists
Abstract | Full Text: HTML PDF
Unit 19.4 Protein Databases on the Internet
Full Text: HTML PDF
Unit 21.5 Isolation of Histones and Nucleosome Cores from Mammalian Cells
Abstract | Full Text: HTML PDF
Supplement 49, January 2000
Unit 14.12 Measurement of In Situ Hybridization
Abstract | Full Text: HTML PDF
Unit 14.13 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis
Abstract | Full Text: HTML PDF
Unit 22.1 Overview of Nucleic Acid Arrays
Abstract | Full Text: HTML PDF
Unit 22.2 Preparation of mRNA for Expression Monitoring
Abstract | Full Text: HTML PDF
Appendix 1J Common Conversion Factors
Abstract | Full Text: HTML PDF
Supplement 48, October 1999
Unit 9.4 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
Full Text: HTML PDF
Unit 10.16 Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
Full Text: HTML PDF
Unit 17.13B Analysis of Glycosaminoglycans with Polysaccharide Lyases
Abstract | Full Text: HTML PDF
Unit 18.9 Phosphopeptide Mapping and Identification of Phosphorylation Sites
Abstract | Full Text: HTML PDF
Unit 21.4 DN ase I and Hydroxyl Radical Characterization of Chromatin Complexes
Abstract | Full Text: HTML PDF
Appendix 3A Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
Abstract | Full Text: HTML PDF
Supplement 47, July 1999
Unit 7.0 Introduction to DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 7.4A DNA Sequencing by the Dideoxy Method
Abstract | Full Text: HTML PDF
Unit 7.4B Dideoxy DNA Sequencing with Chemiluminescent Detection
Abstract | Full Text: HTML PDF
Unit 10.2A One-Dimensional SDS Gel Electrophoresis of Proteins
Full Text: HTML PDF
Unit 10.2B One-Dimensional Electrophoresis Using Nondenaturing Conditions
Abstract | Full Text: HTML PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
Supplement 46, April 1999
Unit 7.1 DNA Sequencing Strategies
Full Text: HTML PDF
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Unit 19.3 Sequence Similarity Searching Using the BLAST Family of Programs
Abstract | Full Text: HTML PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Full Text: HTML PDF
Unit 20.5 Detection of Protein-Protein Interactions by Coprecipitation
Full Text: HTML PDF
Supplement 45, January 1999
Unit 2.7 Separation of Small DNA Fragments by Conventional Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 2.8 Capillary Electrophoresis of DNA
Full Text: HTML PDF
Unit 9.15 Human Somatic Cell Gene Targeting
Unit 9.16 Production of Heterozygous Mutant Cell Line
Unit 9.17 Production of Homozygous Mutant
Unit 21.1 Micrococcal Nuclease Analysis of Chromatin Structure
Full Text: HTML PDF
Unit 21.2 Separation of Histone Variants and Post-Translationally Modified Isoforms by Triton/Acetic Acid/Urea Polyacrylamide Gel Electrophoresis
Abstract | Full Text: HTML PDF
Supplement 44, October 1998
Unit 10.9 Gel-Filtration Chromatography
Abstract | Full Text: HTML PDF
Unit 10.10 Ion-Exchange Chromatography
Abstract | Full Text: HTML PDF
Unit 10.18 Metabolic Labeling with Amino Acids
Abstract | Full Text: HTML PDF
Unit 14.10 Principles and Application of Fluorescence Microscopy
Abstract | Full Text: HTML PDF
Unit 14.11 Basic Confocal Microscopy
Full Text: HTML PDF
Supplement 43, July 1998
Unit 10.12 HPLC of Peptides and Proteins: Preparation and System Set-Up
Unit 16.13 Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
Unit 16.15 Overview of the Vaccinia Virus Expression System
Abstract | Full Text: HTML PDF
Unit 16.16 Preparation of Cell Cultures and Vaccinia Virus Stocks
Abstract | Full Text: HTML PDF
Unit 16.17 Generation of Recombinant Vaccinia Viruses
Abstract | Full Text: HTML PDF
Unit 16.18 Characterization of Recombinant Vaccinia Viruses and Their Products
Abstract | Full Text: HTML PDF
Unit 16.19 Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
Abstract | Full Text: HTML PDF
Appendix 1I Commonly Used Detergents
Abstract | Full Text: HTML PDF
Appendix 3I Statistics for the Molecular Biologist: Group Comparisons
Abstract | Full Text: HTML PDF
Supplement 42, April 1998
Unit 2.1A Purification and Concentration of DNA from Aqueous Solutions
Unit 2.1B Purification of DNA by Anion-Exchange Chromatography
Abstract | Full Text: HTML PDF
Unit 2.2 Preparation of Genomic DNA from Mammalian Tissue
Abstract | Full Text: HTML PDF
Unit 2.11 Synthesis and Purification of Oligonucleotides
Abstract | Full Text: HTML PDF
Unit 2.12 Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 19.1 Internet Basics for Biologists
Unit 19.2 Sequence Databases: Integrated Information Retrieval and Data Submission
Supplement 41, January 1998
Unit 1.5 Introduction to Plasmid Biology
Abstract | Full Text: HTML PDF
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Abstract | Full Text: HTML PDF
Unit 10.5 Overview of Digital Electrophoresis Analysis
Unit 10.15 Purification of Recombinant Proteins and Study of Protein Interaction by Epitope Tagging
Abstract | Full Text: HTML PDF
Unit 10.22 Protein Identification and Characterization by Mass Spectrometry
Abstract | Full Text: HTML PDF
Supplement 40, October 1997
Unit 9.2 Transfection Using DEAE-Dextran
Abstract | Full Text: HTML PDF
Unit 18.7 Assays of Protein Kinases Using Exogenous Substrates
Abstract | Full Text: HTML PDF
Unit 18.8 Permeabilization Strategies to Study Protein Phosphorylation
Abstract | Full Text: HTML PDF
Unit 20.4 Surface Plasmon Resonance for Measurements of Biological Interest
Abstract | Full Text: HTML PDF
Appendix 3G Importing Biological Materials
Abstract | Full Text: HTML PDF
Appendix 3H Kinetic Assay Methods
Abstract | Full Text: HTML PDF
Supplement 39, July 1997
Unit 9.5 Selection of Transfected Mammalian Cells
Full Text: HTML PDF
Unit 10.8 Immunoblotting and Immunodetection
Unit 13.6 Yeast Vectors and Assays for Expression of Cloned Genes
Abstract | Full Text: HTML PDF
Unit 13.9 Manipulation of Plasmids from Yeast Cells
Abstract | Full Text: HTML PDF
Unit 13.10 Manipulation of Cloned Yeast DNA
Abstract | Full Text: HTML PDF
Unit 13.11 Preparation of Yeast DNA
Abstract | Full Text: HTML PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Unit 20.3 Phage-Based Expression Cloning to Identify Interacting Proteins
Abstract | Full Text: HTML PDF
Supplement 38, April 1997
Unit 10.21 Overview of Peptide and Protein Analysis by Mass Spectrometry
Abstract | Full Text: HTML PDF
Unit 16.9 Overview of the Baculovirus Expression System
Unit 16.10 Maintenance of Insect Cell Cultures and Generation of Recombinant Baculoviruses
Unit 16.11 Expression and Purification of Recombinant Proteins Using the Baculovirus System
Unit 16.21 Overview of the HIV-1 Lentiviral Vector System
Supplement 37, January 1997
Unit 1.8 Introduction of Plasmid DNA into Cells
Abstract | Full Text: HTML PDF
Unit 4.9 Analysis of RNA by Northern and Slot Blot Hybridization
Full Text: HTML PDF
Unit 8.5 Directed Mutagenesis Using the Polymerase Chain Reaction
Abstract | Full Text: HTML PDF
Unit 9.12 Large-Scale Preparation and Concentration of Retrovirus Stocks
Abstract | Full Text: HTML PDF
Unit 11.12 In Vitro Antibody Production
Full Text: HTML PDF
Supplement 36, October 1996
Unit 4.2 Guanidine Methods for Total RNA Preparation
Abstract | Full Text: HTML PDF
Unit 9.9 Overview of the Retrovirus Transduction System
Abstract | Full Text: HTML PDF
Unit 9.10 Preparation of a Specific Retrovirus Producer Cell Line
Abstract | Full Text: HTML PDF
Unit 9.11 Transient Transfection Methods for Preparation of High-Titer Retroviral Supernatants
Abstract | Full Text: HTML PDF
Unit 9.13 Detection of Helper Virus in Retrovirus Stocks
Abstract | Full Text: HTML PDF
Unit 9.14 Retrovirus Infection of Cells In Vitro and In Vivo
Abstract | Full Text: HTML PDF
Unit 10.11A Immunoaffinity Chromatography
Abstract | Full Text: HTML PDF
Unit 10.11B Metal-Chelate Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 12.2 Mobility Shift DNA-Binding Assay Using Gel Electrophoresis
Abstract | Full Text: HTML PDF
Supplement 35, July 1996
Unit 10.1A Spectrophotometric and Colorimetric Determination of Protein Concentration
Full Text: HTML PDF
Unit 10.1B Quantitative Amino Acid Analysis
Abstract | Full Text: HTML PDF
Unit 14.9 Whole-Mount In Situ Hybridization and Detection of RNA s in Vertebrate Embryos and Isolated Organs
Unit 18.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides
Abstract | Full Text: HTML PDF
Appendix 2 Commonly Used Reagents and Equipment
Abstract | Full Text: HTML PDF
Appendix 3E Introduction of Restriction Enzyme Recognition Sequences by Silent Mutation
Abstract | Full Text: HTML PDF
Appendix 3F Techniques for Mammalian Cell Tissue Culture
Full Text: HTML PDF
Supplement 34, April 1996
Unit 5.9 Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
Unit 5.10 Amplification of a Bacteriophage Library
Abstract | Full Text: HTML PDF
Unit 5.11 Amplification of Cosmid and Plasmid Libraries
Abstract | Full Text: HTML PDF
Unit 9.7C Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics in Vivo
Abstract | Full Text: HTML PDF
Unit 10.20 Capillary Electrophoresis of Proteins and Peptides
Abstract | Full Text: HTML PDF
Appendix 1H Safe Use of Hazardous Chemicals
Supplement 33, January 1996
Unit 12.11 Determination of Protein-DNA Sequence Specificity by PCR-Assisted Binding-Site Selection
Abstract | Full Text: HTML PDF
Unit 18.1 Overview of Protein Phosphorylation
Abstract | Full Text: HTML PDF
Unit 18.5 Detection of Phosphorylation by Enzymatic Techniques
Abstract | Full Text: HTML PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Unit 20.2 Affinity Purification of Proteins Binding to GST Fusion Proteins
Abstract | Full Text: HTML PDF
Appendix 1A Common Abbreviations
Appendix 1B Useful Measurements and Data
Abstract | Full Text: HTML PDF
Appendix 1C Characteristics of Amino Acids
Abstract | Full Text: HTML PDF
Appendix 1D Characteristics of Nucleic Acids
Abstract | Full Text: HTML PDF
Appendix 1E Radioactivity
Full Text: HTML PDF
Appendix 1F Safe Use of Radioisotopes
Full Text: HTML PDF
Appendix 1G Centrifuges and Rotors
Abstract | Full Text: HTML PDF
Supplement 32, October 1995
Unit 15.7 Quantitation of Rare DNAs by PCR
Unit 17.9 Direct Chemical Analysis of Glycoconjugates for Carbohydrates
Abstract | Full Text: HTML PDF
Unit 17.11 Synthetic Glycosides as Primers of Oligosaccharide Biosynthesis and Inhibitors of Glycoprotein and Proteoglycan Assembly
Abstract | Full Text: HTML PDF
Unit 17.10B Inhibition of Glycolipid Biosynthesis
Abstract | Full Text: HTML PDF
Unit 17.14A Preparation of Glycopeptides
Abstract | Full Text: HTML PDF
Unit 17.14B Detection of Individual Glycosylation Sites on Glycoproteins
Abstract | Full Text: HTML PDF
Unit 17.16 Acid Hydrolysis for Release of Monosaccharides
Abstract | Full Text: HTML PDF
Unit 17.17A Enzymatic Release of Oligosaccharides from Glycolipids
Abstract | Full Text: HTML PDF
Unit 17.17B Endo-β-Galactosidases and Keratanase
Abstract | Full Text: HTML PDF
Unit 17.18 Analysis of Monosaccharides
Abstract | Full Text: HTML PDF
Unit 17.19A Total Compositional Analysis by High-Performance Liquid Chromatography or Gas-Liquid Chromatography
Abstract | Full Text: HTML PDF
Unit 17.19B Composition of Labeled Monosaccharides from Glycosaminoglycans
Abstract | Full Text: HTML PDF
Unit 17.20 Analysis of Oligosaccharide Negative Charge by Anion-Exchange Chromatography
Abstract | Full Text: HTML PDF
Unit 17.21A HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides
Abstract | Full Text: HTML PDF
Unit 17.21B Fractionation and Analysis of Neutral Oligosaccharides by HPLC
Abstract | Full Text: HTML PDF
Unit 17.22A Nitrous Acid Degradation of Glycosaminoglycans
Abstract | Full Text: HTML PDF
Unit 17.22B Analysis of Disaccharides and Tetrasaccharides Released from Glycosaminoglycans
Abstract | Full Text: HTML PDF
Unit 17.23 Analysis of Sulfate Esters by Solvolysis or Hydrolysis
Abstract | Full Text: HTML PDF
Unit 19.3 Sequence Similarity Searching Using the BLAST Family of Programs
Supplement 31, July 1995
Unit 3.1 Digestion of DNA with Restriction Endonucleases
Abstract | Full Text: HTML PDF
Unit 10.2 One-Dimensional SDS Gel Electrophoresis of Proteins
Unit 14.7 In situ Hybridization and Detection Using Nonisotopic Probes
Full Text: HTML PDF
Unit 14.8 In situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets
Full Text: HTML PDF
Unit 17.15B β-Elimination for Release of O -GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides
Abstract | Full Text: HTML PDF
Supplement 30, April 1995
Unit 7.7 Computer Manipulation of DNA and Protein Sequences
Abstract | Full Text: HTML PDF
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Unit 18.1 Overview of Protein Phosphorylation
Unit 18.2 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 18.3 Phosphoamino Acid Analysis
Abstract | Full Text: HTML PDF
Unit 18.4 Analysis of Phosphorylation of Unlabeled Proteins
Abstract | Full Text: HTML PDF
Unit 19.1 Internet Basics for Biologists
Unit 19.2 Sequence Databases: Information Retrieval and Data Submission
Supplement 29, January 1995
Unit 5.5 Conversion of mRNA into Double-Stranded cDNA
Abstract | Full Text: HTML PDF
Unit 9.6 Overview of Genetic Reporter Systems
Abstract | Full Text: HTML PDF
Unit 9.7B Nonisotopic Assays for Reporter Gene Activity
Abstract | Full Text: HTML PDF
Unit 17.15A β-Elimination for Release of O -Linked Glycosaminoglycans from Proteoglycans
Abstract | Full Text: HTML PDF
Appendix 1 Safe Use of Radioisotopes
Appendix 5 Vectors
Supplement 28, October 1994
Unit 16.1 Overview of Protein Expression in E. coli
Abstract | Full Text: HTML PDF
Unit 16.4A Introduction to Expression by Fusion Protein Vectors
Abstract | Full Text: HTML PDF
Unit 16.4B Enzymatic and Chemical Cleavage of Fusion Proteins
Abstract | Full Text: HTML PDF
Unit 16.5 Expression and Purification of lacZ and trpE Fusion Proteins
Abstract | Full Text: HTML PDF
Unit 16.6 Expression and Purification of Maltose-Binding Protein Fusions
Abstract | Full Text: HTML PDF
Unit 16.7 Expression and Purification of Glutathione- S -Transferase Fusion Proteins
Abstract | Full Text: HTML PDF
Unit 16.8 Expression and Purification of Thioredoxin Fusion Proteins
Abstract | Full Text: HTML PDF
Unit 16.10 Preparation of Insect Cell Cultures and Baculovirus Stock
Unit 16.11 Generation of Recombinant Baculoviruses and Analysis of Recombinant Protein Expression
Appendix 3A Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
Appendix 3B Silanizing Glassware
Abstract | Full Text: HTML PDF
Appendix 3C Dialysis and Ultrafiltration
Abstract | Full Text: HTML PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Appendix 3E Introduction of Restriction Enzyme Recognition Sequences by Silent Mutation
Supplement 27, July 1994
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Unit 2.3 Preparation of Genomic DNA from Plant Tissue
Abstract | Full Text: HTML PDF
Unit 6.12 Recombination-Based Assay (RBA) for Screening Bacteriophage Lambda Libraries
Abstract | Full Text: HTML PDF
Unit 13.14 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Unit 17.13B Analysis of Glycosaminoglycans with Polysaccharide Lyases
Supplement 26, April 1994
Unit 2.6 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
Unit 4.10 Identification of Newly Transcribed RNA
Full Text: HTML PDF
Unit 7.4B Dideoxy DNA Sequencing with Chemiluminescent Detection
Unit 15.8 Differential Display of mRNA by CPR
Unit 17.5 Chemical Labeling of Carbohydrates by Oxidation and Sodium Borohydride Reduction
Abstract | Full Text: HTML PDF
Supplement 25, January 1994
Unit 2.1 Purification of DNA Using Glass Beads
Unit 2.7 Separation of Small DNA Fragments by Conventional Gel Electrophoresis
Unit 12.3 Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions
Abstract | Full Text: HTML PDF
Unit 16.20 Expression of Proteins Using Semliki Forest Virus Vectors
Abstract | Full Text: HTML PDF
Supplement 24, October 1993
Unit 2.14 Purification of DNA Using Anion Exchange Chromatography
Unit 3.17 Constructing Recombinant DNA Molecules by the Polymerase Chain Reaction
Full Text: HTML PDF
Unit 10.11B Metal-Chelate Affinity Chromatography
Unit 12.10 Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography
Abstract | Full Text: HTML PDF
Unit 13.11 Preparation of Yeast DNA
Supplement 23, July 1993
Unit 4.9 Analysis of RNA by Northern and Slot Blot Hybridization
Unit 6.11 Use of Monoclonal Antibodies for Expression Cloning
Abstract | Full Text: HTML PDF
Unit 9.15 Overview of Gene Targeting by Homologous Recombination
Unit 9.16 Production of Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
Unit 9.17 Production of Homozygous Mutant Embryonic Stem Cell Line (Double Knockout)
Unit 13.12 Preparation of Yeast RNA
Abstract | Full Text: HTML PDF
Unit 17.7 Lectin Analysis of Proteins Blotted onto Filters
Abstract | Full Text: HTML PDF
Unit 17.14 Preparation of Glycopeptides
Supplement 22, April 1993
Unit 17.1 Special Considerations for Glycoproteins and Their Purification
Abstract | Full Text: HTML PDF
Unit 17.2 Special Considerations for Proteoglycans and Glycosaminoglycans and Their Purification
Abstract | Full Text: HTML PDF
Unit 17.3 Special Considerations for Glycolipids and Their Purification
Abstract | Full Text: HTML PDF
Unit 17.4 Metabolic Radiolabeling of Animal Cell Glycoconjugates
Full Text: HTML PDF
Unit 17.6 Analysis of Saccharide Structure and Function Using Glycotransferases
Unit 17.8 Detection of Glycophospholipid Anchors on Proteins
Abstract | Full Text: HTML PDF
Unit 17.9 Phenol-Sulfuric Acid Assay for Hexoses and Pentoses
Unit 17.10 Inhibition of N-Linked Glycosylation
Unit 17.12 Sialidases
Abstract | Full Text: HTML PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
Supplement 21, January 1993
Unit 2.9A Southern Blotting
Abstract | Full Text: HTML PDF
Unit 2.9B Dot and Slot Blotting of DNA
Abstract | Full Text: HTML PDF
Unit 2.10 Hybridization Analysis of DNA Blots
Abstract | Full Text: HTML PDF
Unit 7.3 Preparation of Templates for DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 7.4 DNA Sequencing by the Dideoxy Method
Unit 11.11 Purification of Monoclonal Antibodies
Abstract | Full Text: HTML PDF
Supplement 20, October 1992
Unit 3.18 Labeling and Colorimetric Detection of Nonisotopic Probes
Abstract | Full Text: HTML PDF
Unit 3.19 Chemiluminescent Detection of Nonisotopic Probes
Abstract | Full Text: HTML PDF
Unit 4.8 Primer Extension
Abstract | Full Text: HTML PDF
Unit 6.9 Overview of Strategies for Screening YAC Libraries and Analyzing YAC Clones
Abstract | Full Text: HTML PDF
Unit 6.10 Analysis of Isolated YAC Clones
Abstract | Full Text: HTML PDF
Unit 10.3 Two-Dimensional Gel Electrophoresis Using the ISO-DALT System
Abstract | Full Text: HTML PDF
Unit 15.5 Ligand-Mediated PCR for Genomic Sequencing and Footprinting
Supplement 19, July 1992
Unit 3.1 Digestion of DNA with Restriction Endonucleases
Unit 7.7 Computer Analysis of DNA and and Protein Sequences
Unit 10.19 Isolation of Proteins for Microsequence Analysis
Abstract | Full Text: HTML PDF
Unit 13.1 Preparation of Yeast Media
Abstract | Full Text: HTML PDF
Unit 13.2 Growth and Manipulation of Yeast
Full Text: HTML PDF
Unit 15.7 Molecular Cloning of PCR Products
Supplement 18, April 1992
Unit 5.8A Production of a Complete cDNA Library
Abstract | Full Text: HTML PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis Using the O'Farrell System
Full Text: HTML PDF
Unit 10.8 Immunoblotting and Immunodetection
Unit 11.3 Isotype Determination of Antibodies
Abstract | Full Text: HTML PDF
Unit 11.4 Immunization of Mice
Abstract | Full Text: HTML PDF
Unit 11.10 Production of Monoclonal Antibody Supernatant and Ascites Fluid
Full Text: HTML PDF
Unit 13.7 Introduction of DNA into Yeast Cells
Abstract | Full Text: HTML PDF
Unit 16.14 Amplification Using CHO Cell Expression Vectors
Unit 16.19 Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
Supplement 17, January 1992
Unit 7.5 DNA Sequencing by the Chemical Method
Abstract | Full Text: HTML PDF
Unit 9.10 Overview of the Retrovirus Transduction System
Unit 9.11 Preparation of a Specific Retrovirus Producer Cell Line
Unit 9.12 Large-Scale Preparation and Concentration of Retrovirus Stocks
Unit 9.13 Detection of Helper Virus in Retrovirus Stocks
Unit 9.14 Retrovirus Infection of Cells In Vitro and In Vivo
Unit 15.6 cDNA Amplification Using One-Sided (Anchored) PCR
Abstract | Full Text: HTML PDF
Supplement 16, October 1991
Unit 7.1 DNA Sequencing Strategies
Unit 7.2 Constructing Nested Deletions for Use in DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 7.3 Preparation of Templates for DNA Sequencing
Unit 7.4 DNA Sequencing by the Dideoxy Method
Unit 7.6 Denaturing Gel Electrophoresis for Sequencing
Abstract | Full Text: HTML PDF
Unit 15.2 Direct DNA Sequencing of PCR Products
Supplement 15, July 1991
Unit 1.6 Minipreps of Plasmid DNA
Abstract | Full Text: HTML PDF
Unit 4.4 Preparation of Bacterial RNA
Abstract | Full Text: HTML PDF
Unit 8.5 Mutagenesis by the Polymerase Chain Reaction
Unit 11.2 Enzyme-Linked Immunosorbent Assays (ELISA) for Detection of Antigens
Abstract | Full Text: HTML PDF
Unit 16.15 Overview of the Vaccinia Virus Expression System
Unit 16.16 Preparation of Cell Cultures and Vaccinia Virus Stocks
Unit 16.17 Generation of Recombinant Vaccinia Viruses
Unit 16.18 Characterization of Recombinant Vaccinia Viruses and Their Products
Supplement 14, April 1991
Unit 4.2 Guanidine Methods for Total RNA Preparation
Unit 9.4 Liposome-Mediated Transfection
Unit 15.5 Ligation-Mediated PCR for Genomic Sequencing and Footprinting
Unit 16.12 Overview of Protein Expression in Mammalian Cells
Unit 16.13 Transient Expression of Proteins Using COS Cells
Supplement 13, January 1991
Unit 5.6 Ligation of Linkers or Adapters to Double-Stranded cDNA
Abstract | Full Text: HTML PDF
Unit 10.2 One-Dimensional Gel Electrophoresis of Proteins
Unit 10.6 Staining Proteins in Gels
Unit 10.18 Biosynthetic Labeling of Proteins
Unit 12.7 Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
Abstract | Full Text: HTML PDF
Unit 14.5 Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides
Abstract | Full Text: HTML PDF
Supplement 12, October 1990
Unit 3.18 Nonisotopic Labeling of DNA Using Nick Translation
Unit 9.6 Reporter System Using Firefly Luciferase
Unit 13.3 Mutagenesis of Yeast Cells
Appendix 1 Characteristics of Amino Acids
Appendix 1 Characteristics of Nucleic Acids
Supplement 11, July 1990
Unit 3.1 Digestion of DNA with Restriction Endonucleases
Unit 9.1 Calcium Phosphate Transfection
Unit 12.1 Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
Full Text: HTML PDF
Unit 16.1 Overview of Protein Expression in E. coli
Unit 16.2 Expression Using the T7 RNA Polymerase/Promoter System
Abstract | Full Text: HTML PDF
Unit 16.3 Expression Using Vectors with Phage Regulatory Sequences
Abstract | Full Text: HTML PDF
Supplement 10, April 1990
Unit 1.13 Preparing Lambda DNA from Phage Lysates
Abstract | Full Text: HTML PDF
Unit 11.1 Conjugation of Enzymes to Antibodies
Abstract | Full Text: HTML PDF
Unit 11.2 Enzyme-Linked Immunosorbent Assays (ELISA) for Detection of Antigens
Unit 16.4 Introduction to Expression by Fusion Protein Vectors
Unit 16.5 Expression and Purification of lacZ and trpE Fusion Proteins
Unit 16.6 Expression and Purification of Maltose-Binding Protein Fusions
Unit 16.7 Expression and Purification of Glutathione- S -Transferase Fusion Proteins
Unit 16.8 Overview of the Baculoviral Expression System
Unit 16.9 Preparation of Insect Cell Cultures and Baculovirus Stock
Unit 16.10 Purification and Transfection of Baculoviral DNA for Generating Recombinant Viruses
Unit 16.11 Generation of Recombinant Baculoviruses and Analysis of Recombinant Protein Expression
Supplement 9, January 1990
Unit 2.9 Electroblotting and UV Crosslinking of DNA
Unit 3.5 DNA-Dependent DNA Polymerases
Full Text: HTML PDF
Unit 4.10 Identification of Newly Transcribed RNA
Unit 9.6 Phase-Extraction Assay for CAT Activity
Unit 13.13 Preparation of Protein Extracts from Yeast
Abstract | Full Text: HTML PDF
Unit 15.1 Enzymatic Amplification of DNA by Polymerase Chain Reaction: Standard Procedures and Optimization
Unit 15.2 Direct DNA Sequencing of Polymerase Chain Reaction Products
Unit 15.3 Quantitation of Rare DNAs by the Polymerase Chain Reaction
Unit 15.4 Enzymatic Amplification of RNA by the Polymerase Chain Reaction
Supplement 8, October 1989
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 2.11 Synthesis of Oligonucleotides
Unit 3.13 Ribonucleases
Abstract | Full Text: HTML PDF
Unit 10.17 Synthesizing Proteins In Vitro by Transcription and Translation of Cloned Genes
Abstract | Full Text: HTML PDF
Unit 12.9 Analysis of DNA-Protein Interactions Using Proteins Synthesized In Vitro from Cloned Genes
Abstract | Full Text: HTML PDF
Appendix 3 Commonly Used Biochemical Techniques
Supplement 7, July 1989
Unit 12.4 DNase I Footprint Analysis of Protein-DNA Binding
Abstract | Full Text: HTML PDF
Unit 14.1 Fixation, Embedding, and Sectioning of Tissues, Embryos, and Single Cells
Abstract | Full Text: HTML PDF
Unit 14.2 Cryosectioning
Abstract | Full Text: HTML PDF
Unit 14.3 In Situ Hybridization to Cellular RNA
Unit 14.4 Detection of Hybridized Probe
Abstract | Full Text: HTML PDF
Unit 14.5 Counterstaining and Mounting of Autoradiographed In Situ Hybridization Slides
Unit 14.6 Immunohistochemistry
Full Text: HTML PDF
Supplement 6, April 1989
Unit 2.9 Southern Blotting and Hybridization
Unit 2.12 Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis
Unit 8.2B Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
Abstract | Full Text: HTML PDF
Unit 11.12 Production of Polyclonal Antisera in Rabbits
Unit 11.13 Purification of Immunoglobin G Fraction from Antiserum, Ascites Fluid, or Hydridoma Supernatant
Unit 11.14 Selection of an Immugenic Peptide
Unit 11.15 Production of Antipeptide Antibodies
Unit 11.16 Determination of the Specific Antibody Titer
Supplement 5, January 1989
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 3.1 Digestion of DNA with Restriction Endonucleases
Unit 13.1 Preparation of Yeast Media
Unit 13.2 Growth and Manipulation of Yeast
Unit 13.4 Yeast Cloning Vectors and Genes
Abstract | Full Text: HTML PDF
Unit 13.6 Yeast Cloning Vectors and Genes
Unit 13.7 Introduction of DNA into Yeast Cells
Unit 13.8 Cloning Yeast Genes by Complementation
Abstract | Full Text: HTML PDF
Unit 13.9 Segregation of Plasmids from Yeast Cells
Unit 13.10 Manipulation of Cloned Yeast DNA
Unit 13.11 Preparation of Yeast DNA
Unit 13.12 Preparation of Yeast RNA
Supplement 4, October 1988
Unit 2.5A Agarose Gel Electrophoresis
Unit 2.5B Pulsed-Field Gel Electrophoresis
Unit 2.10 Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography
Unit 3.5 DNA-Dependent DNA Polymerases
Unit 3.17 The Polymerase Chain Reaction: In Vitro Enzymatic Amplification of DNA
Unit 5.8A Production of a Completed cDNA Library
Unit 5.8B Production of a Subtracted cDNA Library
Supplement 3, July 1988
Unit 12.2 Mobility Shift DNA-Binding Assay Using Gel Electrophoresis
Unit 12.3 Methylation Interference Assay for Analysis of DNA-Protein Interactions
Unit 12.5 UV Crosslinking of Proteins to Nucleic Acids
Abstract | Full Text: HTML PDF
Unit 12.6 Purification of DNA-Binding Proteins Using Biotin/Streptavidin Affinity Systems
Abstract | Full Text: HTML PDF
Unit 12.7 Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
Unit 12.8 Rapid Separation of Protein-Bound DNA from Free DNA Using Nitrocellulose Filters
Abstract | Full Text: HTML PDF
Supplement 2, April 1988
Unit 6.4 Using Synthetic Oligonucleotides as Probes
Abstract | Full Text: HTML PDF
Unit 7.3 Construction of Nested Deletions for DNA Sequencing
Unit 7.4 Sequencing by the Dideoxy Method
Unit 8.4 Linker-Scanning Mutagenesis of DNA
Abstract | Full Text: HTML PDF
Unit 10.15 High-Performance Chromatofocusing and Hydrophobic-Interaction Chromatography
Appendix 1 Standard Measurements, Data, and Abbreviations
Supplement 1, January 1988
Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture Cells
Unit 4.6 S1 Analysis of Messenger RNA Using Single-Stranded DNA Probes
Abstract | Full Text: HTML PDF
Unit 11.1 Conjugation of Enzymes to Antibodies
Unit 11.2 Enzyme-Linked Immunosorbent Assays (ELISA) for Detection of Antigens
Unit 11.3 Immunization of Mice
Unit 11.4 Direct Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Antibodies in Serum and Hybridoma Supernatants
Unit 11.5 Preparation of Myeloma Cells
Abstract | Full Text: HTML PDF
Unit 11.6 Preparation of Mouse Feeder Cells for Fusion and Cloning
Abstract | Full Text: HTML PDF
Unit 11.7 Fusion of Myeloma Cells with Immune Spleen Cells
Abstract | Full Text: HTML PDF
Unit 11.8 Cloning of Hybridoma Cell Lines by Limiting Dilution
Abstract | Full Text: HTML PDF
Unit 11.9 Freezing and Recovery of Hybridoma Cell Lines
Abstract | Full Text: HTML PDF
Unit 11.10 Production of Ascites Fluid
Appendix 3 Commonly Used Biochemical Techniques
Core, 1987
Unit 1.1 Media Preparation and Bacteriological Tools
Unit 1.2 Growth in Liquid Media
Unit 1.3 Growth on Solid Media
Unit 1.4 Selected Topics from Classical Bacterial Genetics
Unit 1.5 Introduction to Plasmid Biology
Unit 1.6 Minipreps of Plasmid DNA
Unit 1.7 CsCl/Ethidium Bromide Preparations of Plasmid DNA
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 1.9 Introduction to Lambda Phages
Abstract | Full Text: HTML PDF
Unit 1.10 Lambda as a Cloning Vector
Abstract | Full Text: HTML PDF
Unit 1.11 Plating Lambda Phage to Generate Plaques
Abstract | Full Text: HTML PDF
Unit 1.12 Growing Lambda-Derived Vectors
Abstract | Full Text: HTML PDF
Unit 1.13 Making Phage DNA from Liquid Lysates
Unit 1.14 Introduction to Vectors Derived from Filamentous Phages
Abstract | Full Text: HTML PDF
Unit 1.15 Preparing and Using M13-Derived Vectors
Abstract | Full Text: HTML PDF
Unit 2.1 Purification and Concentration of DNA from Aqueous Solutions
Unit 2.2 Preparation of Genomic DNA from Mammalian Tissue
Unit 2.3 Preparation of Genomic DNA from Plant Tissue
Unit 2.4 Preparation of Genomic DNA from Bacteria
Unit 2.5 Agarose Gel Electrophoresis
Unit 2.6 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
Unit 2.7 Nondenaturing Polyacrylamide Gel Electrophoresis
Unit 2.8 Sieving Agarose Gel Electrophoresis
Unit 2.9 Southern Blotting and Hybridization
Unit 3.1 Digestion of DNA with Restriction Endonucleases
Unit 3.2 Mapping by Multiple Endonuclease Digestions
Abstract | Full Text: HTML PDF
Unit 3.3 Mapping by Partial Endonuclease Digestions
Abstract | Full Text: HTML PDF
Unit 3.4 Reagents and Radioisotopes Used to Manipulate Nucleic Acids
Abstract | Full Text: HTML PDF
Unit 3.5 DNA-Dependent DNA Polymerases
Unit 3.6 Template-Independent DNA Polymerases
Full Text: HTML PDF
Unit 3.7 RNA-Dependent DNA Polymerases
Full Text: HTML PDF
Unit 3.8 DNA-Dependent RNA Polymerases
Full Text: HTML PDF
Unit 3.9 DNA-Independent RNA Polymerases
Full Text: HTML PDF
Unit 3.10 Phosphatases and Kinases
Abstract | Full Text: HTML PDF
Unit 3.11 Exonucleases
Abstract | Full Text: HTML PDF
Unit 3.12 Endonucleases
Abstract | Full Text: HTML PDF
Unit 3.13 Nonspecific Double-Stranded Endonucleases
Full Text: HTML PDF
Unit 3.14 DNA Ligases
Abstract | Full Text: HTML PDF
Unit 3.15 RNA Ligases
Full Text: HTML PDF
Unit 3.16 Subcloning of DNA Fragments
Abstract | Full Text: HTML PDF
Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture Cells
Unit 4.2 Guanidine Methods for Total RNA Preparation
Unit 4.3 Phenol/ SDS Method for Plant RNA Preparation
Abstract | Full Text: HTML PDF
Unit 4.4 Preparation of Bacterial RNA
Unit 4.5 Preparation of Poly(A) RNA
Abstract | Full Text: HTML PDF
Unit 4.6 S1 Analysis of Messenger RNA Using Single-Stranded DNA Probes
Unit 4.7 Ribonuclease Protection Assay
Abstract | Full Text: HTML PDF
Unit 4.8 Primer Extension
Unit 4.9 Analysis of RNA by Northern Hybridization
Unit 4.10 Identification of Newly Transcribed RNA
Unit 5.1 Genomic DNA Libraries
Abstract | Full Text: HTML PDF
Unit 5.2 cDNA Libraries
Abstract | Full Text: HTML PDF
Unit 5.3 Size Fractionation Using Sucrose Gradients
Abstract | Full Text: HTML PDF
Unit 5.4 Size Fractionation Using Agarose Gels
Abstract | Full Text: HTML PDF
Unit 5.5 Conversion of mRNA into Double-Stranded cDNA
Unit 5.6 Methylation and Addition of Linkers to Double-Stranded cDNA
Unit 5.7 Production of a Genomic Library
Abstract | Full Text: HTML PDF
Unit 5.8 Production of a cDNA Library
Unit 5.9 Amplification of a Bacteriophage Lambda Library
Unit 5.10 Amplification of Cosmid and Plasmid Libraries
Unit 6.1 Plating and Transferring Bacteriophage Libraries
Abstract | Full Text: HTML PDF
Unit 6.2 Plating and Transferring Cosmid and Plasmid Libraries
Abstract | Full Text: HTML PDF
Unit 6.3 Using DNA Fragments as Probes
Abstract | Full Text: HTML PDF
Unit 6.4 Using Synthetic Oligonucleotides as Probes
Unit 6.5 Purification of Bacteriophage Clones
Abstract | Full Text: HTML PDF
Unit 6.6 Purification of Cosmid and Plasmid Clones
Abstract | Full Text: HTML PDF
Unit 6.7 Immunoscreening of Fusion Proteins Produced in Lambda Plaques
Abstract | Full Text: HTML PDF
Unit 6.8 Immunoscreening after Hybrid Selection and Translation
Abstract | Full Text: HTML PDF
Unit 7.1 Overview of DNA Sequencing Strategies
Unit 7.2 Computer-Assisted DNA Sequence Data Entry and DNA Sequence Manipulation
Unit 7.3 Construction of Nested Deletions for DNA Sequencing Using Bal 31 Exonuclease
Unit 7.4 Sequencing by the Dideoxy Method
Unit 7.5 Sequencing by the Chemical Method
Unit 7.6 Rapid DNA Sequencing by the Chemical Degradation Method Utilizing Specialized Vectors for Cloning Nested Deletions
Unit 8.1 Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
Abstract | Full Text: HTML PDF
Unit 8.2 Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
Unit 8.3 Region-Specific Mutagenesis
Unit 9.1 Calcium Phosphate Transfection
Unit 9.2 Transfection Using DEAE-Dextran
Unit 9.3 Transfection by Electroporation
Abstract | Full Text: HTML PDF
Unit 9.4 Optimization of Transfection
Unit 9.5 Stable Transfer of Genes into Mammalian Cells
Unit 9.6 Harvest and Assay for Chloramphenicol Acetyltransferase
Unit 9.7 Assay for Human Growth Hormone
Unit 9.8 Direct Analysis of RNA after Transfection
Abstract | Full Text: HTML PDF
Unit 9.9 Amplification Using Dihydrofolate Reductase
Unit 10.1 Colorimetric Methods
Unit 10.2 One-Dimensional Gel Electrophoresis
Unit 10.3 Two-Dimensional Gel Electrophoresis Using the ISO-DALT System
Unit 10.4 Two-Dimensional Gel Electrophoresis Using the O'Farrell System
Unit 10.5 Electroelution of Proteins from Stained Gels
Unit 10.6 Detection of Protein in Gels
Unit 10.7 Detection of Proteins on Blot Transfer Membranes
Unit 10.8 Western Blotting
Unit 10.9 Gel-Filtration Chromatography
Unit 10.10 Ion-Exchange Chromatography
Unit 10.11 Immunoaffinity Chromatography
Unit 10.12 Reversed-Phase High-Performance Liquid Chromatography
Unit 10.13 Ion-Exchange High-Performance Liquid Chromatography
Unit 10.14 Size-Exclusion High-Performance Liquid Chromatography
Unit 10.15 High-Performance Hydrophobic-Interaction Chromatography
Unit 10.16 Immunoprecipitation
Appendix 1 Standard Abbreviations and Useful Data
Appendix 2 Stock Solutions and Buffers
Appendix 3 Commonly Used Biochemical Techniques