Current Protocols in Molecular Biology
Online ISBN: 9780471142720
Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.
First published in 1987, Current Protocols in Molecular Biology established the gold standard for protocol publications. With regularly updated and new material, this extensive collection of protocols ranges from the most basic techniques for isolating and manipulating nucleic acids to a wide range of advanced and specialized methods. Researchers at all levels of expertise can find well-tested and carefully annotated methods to suit their needs, with sufficient detail to ensure successful duplication in their own laboratories. The novice molecular biologist will find such fundamental methods as streaking cells, extracting nucleic acids, and working with enzymes. Advanced researchers will find methods for analysis of DNA, RNA, proteins, and chromatin; analysis of protein-DNA, protein-RNA, and protein-protein interactions; cloning; sequencing; mutagenesis; immunohistochemistry and in situ hybridization; RNA interference; analysis of gene expression; combinatorial libraries; and even species-specific chapters for yeast and mice.
molecular biologists • biochemists • yeast geneticists • mouse geneticists • immunologists • developmental biologists • cell biologists • neuroscientists
bacterial genetics, chromosomes, cloning, DNA, informatics, molecular biology, model organisms, mutagenesis, PCR, polymerase chain reaction, proteins, RNA, sequencing, transfection, yeast genetics, mouse genetics