Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

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  1. Bioinformatics
  2. Cell Biology
    1. Adhesion
    2. Analysis of Proteins
      1. Assays for Investigating deSUMOylation Enzymes
      2. Biochemical Analysis of Protein SUMOylation
      3. Detection of Proteins on Blot Transfer Membranes
      4. Difference Gel Electrophoresis (DIGE) Using CyDye DIGE Fluor Minimal Dyes
      5. Enzyme-Linked Immunosorbent Assays (ELISA)
      6. Extraction of Proteins from Formalin-Fixed, Paraffin-Embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/Mass Spectrometry Analysis
      7. Gel-Filtration Chromatography
      8. Isolation of Proteins by Heat-Induced Extraction from Formalin-Fixed, Paraffin-Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis
      9. HPLC of Peptides and Proteins: Preparation and System Set-Up
      10. HPLC of Peptides and Proteins: Standard Operating Conditions
      11. Immunoblotting and Immunodetection
      12. Ion-Exchange Chromatography
      13. Isolation of Proteins for Microsequence Analysis
      14. Metabolic Labeling with Amino Acids
      15. Metal-Chelate Affinity Chromatography
      16. One-Dimensional Electrophoresis Using Nondenaturing Conditions
      17. Overview of Digital Electrophoresis Analysis
      18. Protein Identification and Characterization by Mass Spectrometry
      19. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
      20. Quantitative Amino Acid Analysis
      21. Reversed-Phase Isolation of Peptides
      22. Spectrophotometric and Colorimetric Determination of Protein Concentration
      23. Two-Dimensional Gel Electrophoresis
      24. Two-Dimensional Gel Electrophoresis Using the ISO-DALT System
      25. Untargeted Metabolomics
    3. Antibodies as Tools
    4. Apoptosis
    5. Cell Isolation and Culture
    6. Enzyme Assays
    7. Fluorescent Proteins as Probes of Structure and Function
    8. Histology
    9. Labeling Molecules
    10. Lipids and Fatty Acids
    11. Manipulating Gene Expression
    12. Microscopy and Imaging
    13. Molecular Biology Techniques
    14. Molecular Interactions
    15. Molecular Trafficking
    16. Nuclei and Chromosomes
    17. Signal Transduction
    18. Whole Organism and Tissue Analysis
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology