Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
    1. Adhesion
    2. Analysis of Proteins
    3. Antibodies as Tools
    4. Apoptosis
    5. Cancer
    6. Cell Cycle Analysis
    7. Cell Isolation and Culture
    8. Enzyme Assays
    9. Fluorescent Proteins as Probes of Structure and Function
    10. Histology
    11. Labeling Molecules
    12. Lipids and Fatty Acids
    13. Manipulating Gene Expression
    14. Microscopy and Imaging
    15. Molecular Biology Techniques
      1. BioTAP-XL: Cross-linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin-Associated Complexes
      2. Chemiluminescent Detection of Nonisotopic Probes
      3. Constructing Recombinant DNA Molecules by PCR
      4. You have free access to this content
        CRISPR/Cas9-Directed Genome Editing of Cultured Cells
      5. Digestion of DNA with Restriction Endonucleases
      6. DNA Isolation from Mammalian Samples
      7. DNA Ligases
      8. DNA Repair Enzymes
      9. DNA-Dependent DNA Polymerases
      10. Endonucleases
      11. Exonucleases
      12. A Detailed Protocol for Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)
      13. Genome-Wide Annotation and Quantitation of Translation by Ribosome Profiling
      14. Immunoblotting and Immunodetection
      15. Introduction of Restriction Enzyme Recognition Sequences by Silent Mutation
      16. Labeling and Colorimetric Detection of Nonisotopic Probes
      17. Laser Microdissection–Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA
      18. Mapping by Multiple Endonuclease Digestions
      19. Mapping by Partial Endonuclease Digestions
      20. Mapping Networks of Protein-Mediated Physical Interactions Between Chromatin Elements
      21. Mapping Regulatory Factors by Immunoprecipitation from Native Chromatin
      22. Phosphatases and Kinases
      23. Preparation of Genomic DNA from Mammalian Sperm
      24. Protein Interaction Profile Sequencing (PIP-seq)
      25. RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5′-Complete cDNAs
      26. Reagents and Radioisotopes Used to Manipulate Nucleic Acids
      27. Ribonucleases
      28. RNA Isolation from Mammalian Samples
      29. RNA Ligases
      30. RNA Polymerases
      31. RNA-Dependent DNA Polymerases
      32. Selection of Transfected Mammalian Cells
      33. Site-Specific Recombinational Cloning Using Gateway and In-Fusion Cloning Schemes
      34. Size Fractionation Using Agarose Gels
      35. Size Fractionation Using Sucrose Gradients
      36. Stem-Loop RT-qPCR for miRNAs
      37. Subcloning of DNA Fragments
      38. Template-Independent DNA Polymerases
      39. Whole-Mount Histochemical Detection of β-Galactosidase Activity
    16. Molecular Interactions
    17. Molecular Trafficking
    18. Nuclei and Chromosomes
    19. Plant Biology
    20. Signal Transduction
    21. Viruses
    22. Whole Organism and Tissue Analysis
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Gene Expression
  8. Genetics and Genomics
  9. Imaging and Microscopy
  10. Immunology
  11. Intermolecular Interactions
  12. Laboratory Organisms and Animal Models
  13. Microbiology
  14. Microbiology
  15. Molecular Biology
  16. Molecular Biology
  17. Molecular Biology
  18. Mouse Biology
  19. Nucleic Acid Chemistry
  20. Pharmacology and Drug Discovery
  21. Protein Production, Purification, and Analysis
  22. Proteomics
  23. RNA
  24. Safety
  25. Stem Cells
  26. Structural Analysis of Biomolecules
  27. Supporting Lab Techniques
  28. Toxicology

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