Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
    1. Bacteria
    2. Invertebrates
    3. Mammalian
      1. Amplification Using CHO Cell Expression Vectors
      2. Assaying Cell Cycle Status Using Flow Cytometry
      3. Characterization of Recombinant Vaccinia Viruses and Their Products
      4. Detection of Mycoplasma Contamination in Cell Cultures
      5. Eradication of Mycoplasma Contaminations from Cell Cultures
      6. Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
      7. Expression of Proteins Using Semliki Forest Virus Vectors
      8. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      9. Generation of HIV-1-Based Lentiviral Vector Particles
      10. Generation of Recombinant Vaccinia Viruses
      11. Helper-Dependent Adenoviral Vectors
      12. Establishment and Culture of Human Skin Fibroblasts
      13. Human Somatic Cell Gene Targeting
      14. Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
      15. Isolation and Immortalization of Lymphocytes
      16. Label-Free Biomedical Imaging of Lipids by Stimulated Raman Scattering Microscopy
      17. Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation
      18. Preparation, Culture, and Immortalization of Mouse Embryonic Fibroblasts
      19. Mouse Embryonic Stem (ES) Cell Culture
      20. Mouse Embryonic Stem (ES) Cell Isolation
      21. Overview of the Baculovirus Expression System
      22. Overview of the HIV-1 Lentiviral Vector System
      23. Overview of the Vaccinia Virus Expression System
      24. Preparation of Cell Cultures and Vaccinia Virus Stocks
      25. Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
      26. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
      27. Techniques for Mammalian Cell Tissue Culture
      28. Transient Expression of Proteins Using COS Cells
    4. Viruses
    5. Yeasts and Fungi
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology