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Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
    1. Bacteria
    2. Invertebrates
    3. Mammalian
      1. Amplification Using CHO Cell Expression Vectors
      2. Characterization of Recombinant Vaccinia Viruses and Their Products
      3. Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
      4. Expression of Proteins Using Semliki Forest Virus Vectors
      5. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      6. Generation of HIV-1-Based Lentiviral Vector Particles
      7. Generation of Recombinant Vaccinia Viruses
      8. Helper-Dependent Adenoviral Vectors
      9. Establishment and Culture of Human Skin Fibroblasts
      10. Human Somatic Cell Gene Targeting
      11. Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
      12. Isolation and Immortalization of Lymphocytes
      13. Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation
      14. Preparation, Culture, and Immortalization of Mouse Embryonic Fibroblasts
      15. Mouse Embryonic Stem (ES) Cell Culture
      16. Mouse Embryonic Stem (ES) Cell Isolation
      17. Overview of the Baculovirus Expression System
      18. Overview of the HIV-1 Lentiviral Vector System
      19. Overview of the Vaccinia Virus Expression System
      20. Preparation of Cell Cultures and Vaccinia Virus Stocks
      21. Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
      22. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
      23. Techniques for Mammalian Cell Tissue Culture
      24. Transient Expression of Proteins Using COS Cells
    4. Viruses
    5. Yeasts and Fungi
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Molecular Biology
  14. Mouse Biology
  15. Nucleic Acid Chemistry
  16. Pharmacology and Drug Discovery
  17. Protein Production, Purification, and Analysis
  18. Proteomics
  19. RNA
  20. Safety
  21. Stem Cells
  22. Structural Analysis of Biomolecules
  23. Supporting Lab Techniques
  24. Toxicology

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