Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
    1. Detection in Cells and Tissues
    2. Differential Expression
    3. Gene Silencing/RNAi
    4. Microarrays and Data Analysis
    5. Transcription and Translation
      1. Analysis of RNA by Northern and Slot Blot Hybridization
      2. Calcium Phosphate Transfection
      3. Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
      4. Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease
      5. Direct Analysis of RNA after Transfection
      6. A Detailed Protocol for Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)
      7. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      8. Genome-Wide Annotation and Quantitation of Translation by Ribosome Profiling
      9. Guanidine Methods for Total RNA Preparation
      10. Heat-Activatable Primers for Hot-Start PCR and Hot-Start One-Step RT-PCR: Endpoint and Real-Time Experiments
      11. Identification of Newly Transcribed RNA
      12. In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
      13. Isotopic Assays for Reporter Gene Activity
      14. Multiplex Illumina Sequencing Using DNA Barcoding
      15. Native Elongating Transcript Sequencing (NET-seq)
      16. Nonisotopic Assays for Reporter Gene Activity
      17. Normalizing cDNA Libraries
      18. Overview of Genetic Reporter Systems
      19. Parallel WGA and WTA for Comparative Genome and Transcriptome NGS Analysis Using Tiny Cell Numbers
      20. Phenol/SDS Method for Plant RNA Preparation
      21. Preparation of Bacterial RNA
      22. Preparation of Cytoplasmic RNA from Tissue Culture Cells
      23. Preparation of Poly(A)+ RNA
      24. Primer Extension
      25. Quantification of microRNA Expression with Next-Generation Sequencing
      26. RASL-seq for Massively Parallel and Quantitative Analysis of Gene Expression
      27. Ribonuclease Protection Assay
      28. Ribosomal RNA Depletion for Efficient Use of RNA-Seq Capacity
      29. S1 Analysis of Messenger RNA Using Single-Stranded DNA Probes
      30. Analysis of Protein Co-Occupancy by Quantitative Sequential Chromatin Immunoprecipitation
      31. Single-Cell Genome and Transcriptome Sequencing Library Construction Using Combination of MDA and Nextera Library Prep Method
      32. Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
      33. Transfection Using DEAE-Dextran
      34. Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics in Vivo
      35. Whole-Transcriptome Amplification of Single Cells for Next-Generation Sequencing
      36. Yeast Vectors and Assays for Expression of Cloned Genes
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology