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Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
    1. Care, Handling, and Husbandry
    2. Cell, Tissue, and Organ Culture
    3. Generation of Genetic Mutants and Animal Models
      1. Chimeric Mouse Production by Microinjection
      2. Modification and Production of BAC Transgenes
      3. Mouse Colony Management
      4. Mouse Embryo Fibroblast (MEF) Feeder Cell Preparation
      5. Mouse Embryonic Stem (ES) Cell Culture
      6. Mouse Embryonic Stem (ES) Cell Isolation
      7. Overview of Gene Targeting by Homologous Recombination
      8. Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
      9. Production of a Homozygous Mutant Embryonic Stem Cell Line (Double Knockout)
      10. Recombineering-Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
      11. Recombineering-Based Procedure for Creating Cre/loxP Conditional Knockouts in the Mouse
      12. Regulation of Transgene Expression Using Tetracycline
      13. Transgenic Mouse Production By Zygote Injection
      14. Uses of Forward and Reverse Genetics in Mice to Study Gene Function
    4. In Situ Labeling and Detection Methods
    5. Models of Gastrointestinal and Metabolic Disorders
    6. Strategies in Animal Model Experimentation
    7. Surgical Procedures
  12. Microbiology
  13. Molecular Biology
  14. Mouse Biology
  15. Nucleic Acid Chemistry
  16. Pharmacology and Drug Discovery
  17. Protein Production, Purification, and Analysis
  18. Proteomics
  19. RNA
  20. Safety
  21. Stem Cells
  22. Structural Analysis of Biomolecules
  23. Supporting Lab Techniques
  24. Toxicology

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