Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
      1. Amplification of a Bacteriophage Library
      2. Amplification of Cosmid and Plasmid Libraries
      3. Analysis of Isolated YAC Clones
      4. cDNA Libraries
      5. Characterization of Recombinant Vaccinia Viruses and Their Products
      6. Cloning Yeast Genes by Complementation
      7. Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
      8. Construction of Mutant Alleles in Saccharomyces cerevisiae without Cloning: Overview and the Delitto Perfetto Method
      9. Design and Assembly of Large Synthetic DNA Constructs
      10. Directed Mutagenesis Using the Polymerase Chain Reaction
      11. DNA Cloning and Engineering by Uracil Excision
      12. DNA Ligases
      13. Enrichment of Error-Free Synthetic DNA Sequences by CEL I Nuclease
      14. Gene and Genome Construction in Yeast
      15. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      16. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
      17. Genomic DNA Libraries
      18. Growing Lambda-Derived Vectors
      19. Growth and Manipulation of S. pombe
      20. Immunoscreening after Hybrid Selection and Translation
      21. Immunoscreening of Fusion Proteins Produced in Lambda Plaques
      22. Introduction of Plasmid DNA into Cells
      23. Introduction to Lambda Phages
      24. Introduction to Plasmid Biology
      25. Introduction to Vectors Derived from Filamentous Phages
      26. Large-Scale Preparation of Plasmid DNA
      27. Library Generation by Gene Shuffling
      28. Linker-Scanning Mutagenesis of DNA
      29. Maintenance of Insect Cell Cultures and Generation of Recombinant Baculoviruses
      30. Manipulation of Cloned Yeast DNA
      31. Manipulation of Plasmids from Yeast Cells
      32. Minipreps of Plasmid DNA
      33. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      34. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      35. Overview of Strategies for Screening YAC Libraries and Analyzing YAC Clones
      36. Overview of the HIV-1 Lentiviral Vector System
      37. Plating and Transferring Bacteriophage Libraries
      38. Plating and Transferring Cosmid and Plasmid Libraries
      39. Plating Lambda Phage to Generate Plaques
      40. Preparing and Using M13-Derived Vectors
      41. Preparing Lambda DNA from Phage Lysates
      42. Purification of Bacteriophage Clones
      43. Purification of Cosmid and Plasmid Clones
      44. Random Mutagenesis by PCR
      45. Recombination-Based Assay (RBA) for Screening Bacteriophage Lambda Libraries
      46. Recombineering-Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
      47. Recombineering-Based Procedure for Creating Cre/loxP Conditional Knockouts in the Mouse
      48. Transfection by Electroporation
      49. Use of Monoclonal Antibodies for Expression Cloning
      50. Using DNA Fragments as Probes
      51. Using Synthetic Oligonucleotides as Probes
      52. Yeast Cloning Vectors and Genes
      53. Yeast Vectors and Assays for Expression of Cloned Genes
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology

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