Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
      1. E. coli Genome Manipulation by P1 Transduction
      2. Enzymatic and Chemical Cleavage of Fusion Proteins
      3. Expression and Purification of Glutathione-S-Transferase Fusion Proteins
      4. Expression and Purification of lacZ and trpE Fusion Proteins
      5. Expression and Purification of Maltose-Binding Protein Fusions
      6. Expression and Purification of Thioredoxin Fusion Proteins
      7. Expression Using the T7 RNA Polymerase/Promoter System
      8. Expression Using Vectors with Phage λ Regulatory Sequences
      9. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      10. Growing Lambda-Derived Vectors
      11. Growth in Liquid Media
      12. Growth on Solid Media
      13. Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
      14. Introduction of Plasmid DNA into Cells
      15. Introduction to Expression by Fusion Protein Vectors
      16. Introduction to Lambda Phages
      17. Introduction to Plasmid Biology
      18. Introduction to Vectors Derived from Filamentous Phages
      19. Lambda as a Cloning Vector
      20. Large-Scale Preparation of Plasmid DNA
      21. Media Preparation and Bacteriological Tools
      22. Minipreps of Plasmid DNA
      23. Overview of Post Cohen-Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly
      24. Overview of Protein Expression in E. coli
      25. Phage-Based Expression Cloning to Identify Interacting Proteins
      26. Plating Lambda Phage to Generate Plaques
      27. Preparation of Poly(A)+ RNA
      28. Preparation of Templates for DNA Sequencing
      29. Preparing and Using M13-Derived Vectors
      30. Preparing Lambda DNA from Phage Lysates
      31. Production of a Complete cDNA Library
      32. Production of a Genomic DNA Library
      33. Selected Topics from Classical Bacterial Genetics
      34. Site-Specific Recombinational Cloning Using Gateway and In-Fusion Cloning Schemes
      35. Transient Expression of Proteins Using COS Cells
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology