Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Detecting Mutations and Variants
  8. Gene Expression
  9. Genetics and Genomics
  10. High-Throughput Sequencing
  11. Imaging and Microscopy
  12. Immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Magnetic Resonance Imaging
  16. Microbiology
  17. Microbiology
  18. Molecular Biology
  19. Molecular Biology
  20. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. CRISPR-mediated gene editing
    6. DNA-Protein Interactions
    7. E. coli, Plasmids, and Bacteriophages
    8. Enzymatic Manipulation of DNA and RNA
      1. Constructing Recombinant DNA Molecules by PCR
      2. Conversion of mRNA into Double-Stranded cDNA
      3. Database of DNA Polymerases
      4. Design and Assembly of Large Synthetic DNA Constructs
      5. Dideoxy DNA Sequencing with Chemiluminescent Detection
      6. Digestion of DNA with Restriction Endonucleases
      7. Directed Mutagenesis Using the Polymerase Chain Reaction
      8. DNA Ligases
      9. DNA Repair Enzymes
      10. DNA Sequencing by the Dideoxy Method
      11. DNA-Dependent DNA Polymerases
      12. Endonucleases
      13. Engineering Designer Nucleases with Customized Cleavage Specificities
      14. Enrichment of Error-Free Synthetic DNA Sequences by CEL I Nuclease
      15. Exome Sequencing by Targeted Enrichment
      16. Exonucleases
      17. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      18. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
      19. Genome Editing in Human Cells Using CRISPR/Cas Nucleases
      20. Helicase-Dependent Amplification of Nucleic Acids
      21. Introduction of Restriction Enzyme Recognition Sequences by Silent Mutation
      22. Labeling and Colorimetric Detection of Nonisotopic Probes
      23. Ligation of Linkers or Adapters to Double-Stranded cDNA
      24. Linker-Scanning Mutagenesis of DNA
      25. Loop-Mediated Isothermal Amplification for Detection of Nucleic Acids
      26. Mapping by Multiple Endonuclease Digestions
      27. Mapping by Partial Endonuclease Digestions
      28. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      29. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      30. Overview of Post Cohen-Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly
      31. Phosphatases and Kinases
      32. Preparation of Templates for DNA Sequencing
      33. Pyrosequencing: Powerful and Quantitative Sequencing Technology
      34. Random Mutagenesis by PCR
      35. Random-Primed, Phi29 DNA Polymerase-Based Whole Genome Amplification
      36. Reagents and Radioisotopes Used to Manipulate Nucleic Acids
      37. Ribonucleases
      38. RNA Ligases
      39. RNA Polymerases
      40. RNA-Dependent DNA Polymerases
      41. Site-Specific Recombinational Cloning Using Gateway and In-Fusion Cloning Schemes
      42. Subcloning of DNA Fragments
      43. Synthesis and Labeling of RNA In Vitro
      44. Template-Independent DNA Polymerases
      45. Using DNA Fragments as Probes
      46. Using Synthetic Oligonucleotides as Probes
      47. Whole-Genome Amplification of Single-Cell Genomes for Next-Generation Sequencing
    9. Expression Analysis
    10. Gene Editing
    11. Gene Expression
    12. Gene Regulation
    13. Gene Silencing
    14. Informatics
    15. Introduction of DNA into Cells
    16. Labeling Methods
    17. Libraries
    18. Molecular Interactions
    19. Mouse Methods
    20. Mutagenesis
    21. Nucleic Acid Arrays
    22. Polymerase Chain Reaction
    23. Post-Translational Modification
    24. Preparation and Analysis of DNA and RNA
    25. Protein Analysis
    26. Protein Expression
    27. Recombinant DNA Libraries
    28. RNA-Protein Interactions
    29. Sequencing
    30. Yeast Methods
  21. Mouse Biology
  22. Mutagenesis
  23. Mutagenesis
  24. Neuroscience
  25. Nucleic Acid Chemistry
  26. Pharmacology and Drug Discovery
  27. Protein Production, Purification, and Analysis
  28. Proteomics
  29. RNA
  30. Safety
  31. Sequencing
  32. Stem Cells
  33. Structural Analysis of Biomolecules
  34. Supporting Lab Techniques
  35. Toxicology

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