Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
      1. AFLP-Based Transcript Profiling
      2. Bisulfite Sequencing of DNA
      3. Characterization of Recombinant Vaccinia Viruses and Their Products
      4. Conditional Depletion of Nuclear Proteins by the Anchor Away System
      5. Detection of Hybridized Probe
      6. Differential Display of mRNA by PCR
      7. Directed Mutagenesis Using the Polymerase Chain Reaction
      8. A Detailed Protocol for Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)
      9. Gene Expression Analysis of a Single or Few Cells
      10. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      11. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
      12. Helicase-Dependent Amplification of Nucleic Acids
      13. Helper-Dependent Adenoviral Vectors
      14. In Situ Hybridization and Detection Using Nonisotopic Probes
      15. In Situ Hybridization to Cellular RNA
      16. In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets
      17. In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
      18. Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
      19. Laser Capture Microdissection
      20. Linker-Scanning Mutagenesis of DNA
      21. Loop-Mediated Isothermal Amplification for Detection of Nucleic Acids
      22. Manipulation of Cloned Yeast DNA
      23. Manipulation of Plasmids from Yeast Cells
      24. Multiplex Illumina Sequencing Using DNA Barcoding
      25. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      26. Native Elongating Transcript Sequencing (NET-seq)
      27. Normalizing cDNA Libraries
      28. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      29. Overview of the HIV-1 Lentiviral Vector System
      30. Overview of the Vaccinia Virus Expression System
      31. Paired-End diTagging for Transcriptome and Genome Analysis
      32. PCR-Based Subtractive cDNA Cloning
      33. Preparation of Protein Extracts from Yeast
      34. Preparation of Single Cells from Solid Tissues for Analysis by PCR
      35. Production of a Subtracted cDNA Library
      36. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
      37. RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5′-Complete cDNAs
      38. Random Mutagenesis by PCR
      39. RASL-seq for Massively Parallel and Quantitative Analysis of Gene Expression
      40. Representational Difference Analysis
      41. Restriction-Mediated Differential Display (RMDD)
      42. Genome-Wide Annotation and Quantitation of Translation by Ribosome Profiling
      43. RNA Immunoprecipitation for Determining RNA-Protein Associations In Vivo
      44. Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
      45. Visualizing Genomes with Oligopaint FISH Probes
      46. Whole-Mount In Situ Hybridization and Detection of RNAs in Vertebrate Embryos and Isolated Organs
      47. Yeast Cloning Vectors and Genes
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology