Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
      1. AFLP-Based Transcript Profiling
      2. Bisulfite Sequencing of DNA
      3. Characterization of Recombinant Vaccinia Viruses and Their Products
      4. Conditional Depletion of Nuclear Proteins by the Anchor Away System
      5. Detection of Hybridized Probe
      6. Differential Display of mRNA by PCR
      7. Directed Mutagenesis Using the Polymerase Chain Reaction
      8. A Detailed Protocol for Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE)
      9. Gene Expression Analysis of a Single or Few Cells
      10. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      11. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
      12. Genome-Wide Annotation and Quantitation of Translation by Ribosome Profiling
      13. Helicase-Dependent Amplification of Nucleic Acids
      14. Helper-Dependent Adenoviral Vectors
      15. In Situ Hybridization and Detection Using Nonisotopic Probes
      16. In Situ Hybridization to Cellular RNA
      17. In Situ Polymerase Chain Reaction and Hybridization to Detect Low-Abundance Nucleic Acid Targets
      18. In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
      19. Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
      20. Laser Microdissection–Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA
      21. Linker-Scanning Mutagenesis of DNA
      22. Loop-Mediated Isothermal Amplification for Detection of Nucleic Acids
      23. Manipulation of Cloned Yeast DNA
      24. Manipulation of Plasmids from Yeast Cells
      25. Mapping 3′ mRNA Isoforms on a Genomic Scale
      26. Multiplex Illumina Sequencing Using DNA Barcoding
      27. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      28. Native Elongating Transcript Sequencing (NET-seq)
      29. Normalizing cDNA Libraries
      30. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      31. Overview of the HIV-1 Lentiviral Vector System
      32. Overview of the Vaccinia Virus Expression System
      33. Paired-End diTagging for Transcriptome and Genome Analysis
      34. PCR-Based Subtractive cDNA Cloning
      35. Preparation of Protein Extracts from Yeast
      36. Preparation of Single Cells from Solid Tissues for Analysis by PCR
      37. Production of a Subtracted cDNA Library
      38. Production of Recombinant Adeno-Associated Viral Vectors for In Vitro and In Vivo Use
      39. RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5′-Complete cDNAs
      40. Random Mutagenesis by PCR
      41. RASL-seq for Massively Parallel and Quantitative Analysis of Gene Expression
      42. Representational Difference Analysis
      43. Restriction-Mediated Differential Display (RMDD)
      44. RNA Immunoprecipitation for Determining RNA-Protein Associations In Vivo
      45. Selective Depletion of Abundant RNAs to Enable Transcriptome Analysis of Low-Input and Highly Degraded Human RNA
      46. Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
      47. Transcriptome-Wide Identification of Pseudouridine Modifications Using Pseudo-seq
      48. Visualizing Genomes with Oligopaint FISH Probes
      49. Whole-Mount In Situ Hybridization and Detection of RNAs in Vertebrate Embryos and Isolated Organs
      50. Yeast Cloning Vectors and Genes
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology

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