Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Gene Expression
  8. Genetics and Genomics
  9. Imaging and Microscopy
  10. Immunology
  11. Intermolecular Interactions
  12. Laboratory Organisms and Animal Models
  13. Microbiology
  14. Microbiology
  15. Molecular Biology
  16. Molecular Biology
  17. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
      1. Construction of Mutant Alleles in Saccharomyces cerevisiae without Cloning: Overview and the Delitto Perfetto Method
      2. You have free access to this content
        CRISPR-Cas9-Guided Genome Engineering in C. elegans
      3. You have free access to this content
        CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants
      4. You have free access to this content
        CRISPR/Cas9-Directed Genome Editing of Cultured Cells
      5. Design and Assembly of Large Synthetic DNA Constructs
      6. Engineering Designer Transcription Activator--Like Effector Nucleases (TALENs) by REAL or REAL-Fast Assembly
      7. Directed Mutagenesis Using the Polymerase Chain Reaction
      8. EMS and UV Mutagenesis in Yeast
      9. Engineering Designer Nucleases with Customized Cleavage Specificities
      10. Engineering Customized TALE Nucleases (TALENs) and TALE Transcription Factors by Fast Ligation-Based Automatable Solid-Phase High-Throughput (FLASH) Assembly
      11. Enrichment of Error-Free Synthetic DNA Sequences by CEL I Nuclease
      12. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
      13. Genome Editing in Human Cells Using CRISPR/Cas Nucleases
      14. Genome-Wide Transposon Mutagenesis in Yeast
      15. You have free access to this content
        Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology
      16. Linker-Scanning Mutagenesis of DNA
      17. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      18. Next-Gen Sequencing-Based Mapping and Identification of Ethyl Methanesulfonate-Induced Mutations in Arabidopsis thaliana
      19. Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans
      20. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      21. Overview of Post Cohen-Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly
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        Practical Considerations for Using Pooled Lentiviral CRISPR Libraries
      23. Random Mutagenesis by PCR
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  18. Mouse Biology
  19. Nucleic Acid Chemistry
  20. Pharmacology and Drug Discovery
  21. Protein Production, Purification, and Analysis
  22. Proteomics
  23. RNA
  24. Safety
  25. Stem Cells
  26. Structural Analysis of Biomolecules
  27. Supporting Lab Techniques
  28. Toxicology