Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
      1. Construction of Mutant Alleles in Saccharomyces cerevisiae without Cloning: Overview and the Delitto Perfetto Method
      2. You have free access to this content
        CRISPR-Cas9-Guided Genome Engineering in C. elegans
      3. You have free access to this content
        CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants
      4. You have free access to this content
        CRISPR/Cas9-Directed Genome Editing of Cultured Cells
      5. Design and Assembly of Large Synthetic DNA Constructs
      6. Engineering Designer Transcription Activator--Like Effector Nucleases (TALENs) by REAL or REAL-Fast Assembly
      7. Directed Mutagenesis Using the Polymerase Chain Reaction
      8. EMS and UV Mutagenesis in Yeast
      9. Engineering Designer Nucleases with Customized Cleavage Specificities
      10. Engineering Customized TALE Nucleases (TALENs) and TALE Transcription Factors by Fast Ligation-Based Automatable Solid-Phase High-Throughput (FLASH) Assembly
      11. Enrichment of Error-Free Synthetic DNA Sequences by CEL I Nuclease
      12. Gene Synthesis: Assembly of Target Sequences Using Mutually Priming Long Oligonucleotides
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        Genome Editing in Human Cells Using CRISPR/Cas Nucleases
      14. Genome-Wide Transposon Mutagenesis in Yeast
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        Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology
      16. Linker-Scanning Mutagenesis of DNA
      17. Mutagenesis with Degenerate Oligonucleotides: Creating Numerous Mutations in a Small DNA Sequence
      18. Next-Gen Sequencing-Based Mapping and Identification of Ethyl Methanesulfonate-Induced Mutations in Arabidopsis thaliana
      19. Oligonucleotide-Directed Mutagenesis without Phenotypic Selection
      20. Overview of Post Cohen-Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly
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        Practical Considerations for Using Pooled Lentiviral CRISPR Libraries
      22. Random Mutagenesis by PCR
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology