Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
    1. Carbohydrate Analysis
    2. Chromatin Assembly and Analysis
    3. Cloning and Recombination Methods
    4. Combinatorial Methods
    5. DNA-Protein Interactions
    6. E. coli, Plasmids, and Bacteriophages
    7. Enzymatic Manipulation of DNA and RNA
    8. Expression Analysis
    9. Gene Expression
    10. Gene Regulation
    11. Gene Silencing
    12. Informatics
    13. Introduction of DNA into Cells
    14. Labeling Methods
    15. Libraries
    16. Molecular Interactions
    17. Mouse Methods
    18. Mutagenesis
    19. Nucleic Acid Arrays
    20. Polymerase Chain Reaction
    21. Post-Translational Modification
    22. Preparation and Analysis of DNA and RNA
    23. Protein Analysis
      1. Analysis of Phosphorylation of Unlabeled Proteins
      2. Analysis of Protein Tyrosine Phosphatases and Substrates
      3. Assays of Protein Kinases Using Exogenous Substrates
      4. Basic Protein Sequence Analysis
      5. BioTAP-XL: Cross-linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin-Associated Complexes
      6. Capillary Electrophoresis of Proteins and Peptides
      7. Characteristics of Amino Acids
      8. Design and Use of Analog-Sensitive Protein Kinases
      9. Detection of Phosphorylation by Enzymatic Techniques
      10. Detection of Proteins on Blot Transfer Membranes
      11. Difference Gel Electrophoresis (DIGE) Using CyDye DIGE Fluor Minimal Dyes
      12. Enzyme-Linked Immunosorbent Assays (ELISA)
      13. Extraction of Proteins from Formalin-Fixed, Paraffin-Embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/Mass Spectrometry Analysis
      14. Gel-Filtration Chromatography
      15. Isolation of Proteins by Heat-Induced Extraction from Formalin-Fixed, Paraffin-Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis
      16. HPLC of Peptides and Proteins: Preparation and System Set-Up
      17. HPLC of Peptides and Proteins: Standard Operating Conditions
      18. Immunoaffinity Chromatography
      19. Immunoblotting and Immunodetection
      20. Immunoprecipitation
      21. Analysis of Proteins
      22. Introduction to Peptide Synthesis
      23. Ion-Exchange Chromatography
      24. Isolation of Phosphopeptides by Immobilized Metal Ion Affinity Chromatography
      25. Isolation of Proteins for Microsequence Analysis
      26. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
      27. Metabolic Labeling with Amino Acids
      28. Metal-Chelate Affinity Chromatography
      29. One-Dimensional Electrophoresis Using Nondenaturing Conditions
      30. One-Dimensional SDS Gel Electrophoresis of Proteins
      31. Overview of Digital Electrophoresis Analysis
      32. Overview of Protein Phosphorylation
      33. Parallel Protein Detection by Solid-Phase Proximity Ligation Assay with Real-Time PCR or Sequencing
      34. Pathway Modulators and Inhibitors
      35. Permeabilization Strategies to Study Protein Phosphorylation
      36. Using Phospho-Motif Antibodies to Determine Kinase Substrates
      37. Phosphoamino Acid Analysis
      38. Phosphopeptide Mapping and Identification of Phosphorylation Sites
      39. Preparation of Protein Extracts from Yeast
      40. Preparation of Proteins and Peptides for Mass Spectrometry Analysis in a Bottom-Up Proteomics Workflow
      41. Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides
      42. Protein Databases on the Internet
      43. Protein Identification and Characterization by Mass Spectrometry
      44. Purification of Recombinant Proteins from Cultured Mammalian Cells by HaloTag Technology
      45. Quantitative Amino Acid Analysis
      46. Quantitative Analysis of Protein-Protein Interactions by Split Firefly Luciferase Complementation in Plant Protoplasts
      47. Endoglycosidase and Glycoamidase Release of N-Linked Glycans
      48. Reversed-Phase Isolation of Peptides
      49. Separation of Histone Variants and Post-Translationally Modified Isoforms by Triton/Acetic Acid/Urea Polyacrylamide Gel Electrophoresis
      50. Solution Radioimmunoassay of Proteins and Peptides
      51. Spectrophotometric and Colorimetric Determination of Protein Concentration
      52. Staining Proteins in Gels
      53. The Detection of MAPK Signaling
      54. Two-Dimensional Gel Electrophoresis
      55. Two-Dimensional Gel Electrophoresis Using the ISO-DALT System
      56. Use of Protein Phosphatase Inhibitors
    24. Protein Expression
    25. Recombinant DNA Libraries
    26. RNA-Protein Interactions
    27. Sequencing
    28. Yeast Methods
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology