Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
    1. Affinity Purification
      1. Affinity Purification of Proteins Binding to GST Fusion Proteins
      2. Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
      3. Expression and Purification of Glutathione-S-Transferase Fusion Proteins
      4. Expression and Purification of lacZ and trpE Fusion Proteins
      5. Expression and Purification of Maltose-Binding Protein Fusions
      6. Expression and Purification of Thioredoxin Fusion Proteins
      7. Identification of Polyol-Responsive Monoclonal Antibodies for Use in Immunoaffinity Chromatography
      8. Immunoaffinity Chromatography
      9. Immunoprecipitation
      10. Isolation of Phosphopeptides by Immobilized Metal Ion Affinity Chromatography
      11. Metal-Chelate Affinity Chromatography
      12. Preparation of Proteins and Peptides for Mass Spectrometry Analysis in a Bottom-Up Proteomics Workflow
      13. Purification of DNA-Binding Proteins Using Biotin/Streptavidin Affinity Systems
      14. Purification of Immunoglobulin G Fraction from Antiserum, Ascites Fluid, or Hybridoma Supernatant
      15. Purification of Monoclonal Antibodies
      16. Purification of Recombinant Proteins and Study of Protein Interaction by Epitope Tagging
      17. Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography
    2. Conventional Chromatography
    3. Detection and Assay Methods
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptides
    7. Production of Recombinant Proteins
    8. Protein Engineering
    9. Purification of Recombinant Proteins
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology

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