Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
      1. Analysis of Phosphorylation of Unlabeled Proteins
      2. Analysis of Serine-Threonine Kinase Specificity Using Arrayed Positional Scanning Peptide Libraries
      3. Analysis of Protein Tyrosine Phosphatases and Substrates
      4. Characterization of Recombinant Vaccinia Viruses and Their Products
      5. Design and Use of Analog-Sensitive Protein Kinases
      6. Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
      7. Detection of Phosphorylation by Enzymatic Techniques
      8. Detection of Protein-Protein Interactions by Coprecipitation
      9. Detection of Proteins on Blot Transfer Membranes
      10. Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
      11. Determination of Protein-DNA Sequence Specificity by PCR-Assisted Binding-Site Selection
      12. DNase I Footprint Analysis of Protein-DNA Binding
      13. Fluorescent Peptide Assays for Protein Kinases
      14. Immunization of Mice
      15. Immunoblotting and Immunodetection
      16. Inhibition of N-Linked Glycosylation
      17. Analysis of Proteins
      18. Isotype Determination of Antibodies
      19. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
      20. Metabolic Labeling with Amino Acids
      21. Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions
      22. Mobility Shift DNA-Binding Assay Using Gel Electrophoresis
      23. One-Dimensional SDS Gel Electrophoresis of Proteins
      24. Overview of Protein Phosphorylation
      25. Parallel Protein Detection by Solid-Phase Proximity Ligation Assay with Real-Time PCR or Sequencing
      26. Pathway Modulators and Inhibitors
      27. Permeabilization Strategies to Study Protein Phosphorylation
      28. Using Phospho-Motif Antibodies to Determine Kinase Substrates
      29. Phosphoamino Acid Analysis
      30. Phosphopeptide Mapping and Identification of Phosphorylation Sites
      31. Quantitative Amino Acid Analysis
      32. Rapid Separation of Protein-Bound DNA from Free DNA Using Nitrocellulose Filters
      33. Serine/Threonine Protein Phosphatase Assays
      34. Solution Radioimmunoassay of Proteins and Peptides
      35. Spectrophotometric and Colorimetric Determination of Protein Concentration
      36. Staining Proteins in Gels
      37. Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
      38. The Detection of MAPK Signaling
      39. The Combined Use of the Thermofluor Assay and ThermoQ Analytical Software for the Determination of Protein Stability and Buffer Optimization as an Aid in Protein Crystallization
      40. Use of Protein Phosphatase Inhibitors
      41. UV Crosslinking of Proteins to Nucleic Acids
      42. Yeast One-Hybrid Screening for DNA-Protein Interactions
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptides
    7. Production of Recombinant Proteins
    8. Protein Engineering
    9. Purification of Recombinant Proteins
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology