Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Detecting Mutations and Variants
  8. Gene Expression
  9. Genetics and Genomics
  10. High-Throughput Sequencing
  11. Imaging and Microscopy
  12. Immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Magnetic Resonance Imaging
  16. Microbiology
  17. Microbiology
  18. Molecular Biology
  19. Molecular Biology
  20. Molecular Biology
  21. Mouse Biology
  22. Mutagenesis
  23. Mutagenesis
  24. Neuroscience
  25. Nucleic Acid Chemistry
  26. Pharmacology and Drug Discovery
  27. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
      1. Analysis of Phosphorylation of Unlabeled Proteins
      2. Analysis of Serine-Threonine Kinase Specificity Using Arrayed Positional Scanning Peptide Libraries
      3. Analysis of Protein Tyrosine Phosphatases and Substrates
      4. Characterization of Recombinant Vaccinia Viruses and Their Products
      5. Design and Use of Analog-Sensitive Protein Kinases
      6. Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
      7. Detection of Phosphorylation by Enzymatic Techniques
      8. Detection of Protein-Protein Interactions by Coprecipitation
      9. Detection of Proteins on Blot Transfer Membranes
      10. Detection, Purification, and Characterization of cDNA Clones Encoding DNA-Binding Proteins
      11. Determination of Protein-DNA Sequence Specificity by PCR-Assisted Binding-Site Selection
      12. DNase I Footprint Analysis of Protein-DNA Binding
      13. Fluorescent Peptide Assays for Protein Kinases
      14. Inhibition of N-Linked Glycosylation
      15. Analysis of Proteins
      16. Isotype Determination of Antibodies
      17. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
      18. Metabolic Labeling with Amino Acids
      19. Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions
      20. Mobility Shift DNA-Binding Assay Using Gel Electrophoresis
      21. One-Dimensional SDS Gel Electrophoresis of Proteins
      22. Overview of Protein Phosphorylation
      23. Parallel Protein Detection by Solid-Phase Proximity Ligation Assay with Real-Time PCR or Sequencing
      24. Pathway Modulators and Inhibitors
      25. Permeabilization Strategies to Study Protein Phosphorylation
      26. Using Phospho-Motif Antibodies to Determine Kinase Substrates
      27. Phosphoamino Acid Analysis
      28. Phosphopeptide Mapping and Identification of Phosphorylation Sites
      29. Quantitative Amino Acid Analysis
      30. Rapid Separation of Protein-Bound DNA from Free DNA Using Nitrocellulose Filters
      31. Serine/Threonine Protein Phosphatase Assays
      32. Solution Radioimmunoassay of Proteins and Peptides
      33. Spectrophotometric and Colorimetric Determination of Protein Concentration
      34. Staining Proteins in Gels
      35. Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
      36. The Detection of MAPK Signaling
      37. The Combined Use of the Thermofluor Assay and ThermoQ Analytical Software for the Determination of Protein Stability and Buffer Optimization as an Aid in Protein Crystallization
      38. Use of Protein Phosphatase Inhibitors
      39. UV Crosslinking of Proteins to Nucleic Acids
      40. Yeast One-Hybrid Screening for DNA-Protein Interactions
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptides
    7. Production of Recombinant Proteins
    8. Protein Engineering
    9. Purification of Recombinant Proteins
  28. Proteomics
  29. RNA
  30. Safety
  31. Sequencing
  32. Stem Cells
  33. Structural Analysis of Biomolecules
  34. Supporting Lab Techniques
  35. Toxicology

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