Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Online ISBN: 9780471142720

DOI: 10.1002/0471142727

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Chemical Biology
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Intermolecular Interactions
  11. Laboratory Organisms and Animal Models
  12. Microbiology
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Mouse Biology
  17. Nucleic Acid Chemistry
  18. Pharmacology and Drug Discovery
  19. Protein Production, Purification, and Analysis
    1. Affinity Purification
    2. Conventional Chromatography
    3. Detection and Assay Methods
    4. Electrophoresis
    5. Extraction, Stabilization, and Concentration
    6. Peptides
    7. Production of Recombinant Proteins
      1. Affinity Purification of Proteins Binding to GST Fusion Proteins
      2. Amplification Using CHO Cell Expression Vectors
      3. Analysis of DNA-Protein Interactions Using Proteins Synthesized In Vitro from Cloned Genes
      4. Characterization of Recombinant Vaccinia Viruses and Their Products
      5. Enzymatic and Chemical Cleavage of Fusion Proteins
      6. Expression and Purification of Epitope-Tagged Multisubunit Protein Complexes from Mammalian Cells
      7. Expression and Purification of Glutathione-S-Transferase Fusion Proteins
      8. Expression and Purification of lacZ and trpE Fusion Proteins
      9. Expression and Purification of Maltose-Binding Protein Fusions
      10. Expression and Purification of Recombinant Proteins Using the Baculovirus System
      11. Expression and Purification of Thioredoxin Fusion Proteins
      12. Expression of Proteins Using Semliki Forest Virus Vectors
      13. Expression Using the T7 RNA Polymerase/Promoter System
      14. Expression Using Vectors with Phage λ Regulatory Sequences
      15. Gene Expression Using the Vaccinia Virus/T7 RNA Polymerase Hybrid System
      16. Generation of Recombinant Vaccinia Viruses
      17. Introduction to Expression by Fusion Protein Vectors
      18. Overview of Cell-Free Protein Synthesis: Historic Landmarks, Commercial Systems, and Expanding Applications
      19. Overview of Protein Expression in E. coli
      20. Overview of the Baculovirus Expression System
      21. Overview of the Vaccinia Virus Expression System
      22. Peptide Aptamers: Dominant “Genetic” Agents for Forward and Reverse Analysis of Cellular Processes
      23. Preparation of Cell Cultures and Vaccinia Virus Stocks
      24. Production of Disulfide-Bonded Proteins in Escherichia coli
      25. Protein Synthesis Using a Reconstituted Cell-Free System
      26. Selection of Transfected Mammalian Cells
      27. Transient Expression of Proteins Using COS Cells
    8. Protein Engineering
    9. Purification of Recombinant Proteins
  20. Proteomics
  21. RNA
  22. Safety
  23. Stem Cells
  24. Structural Analysis of Biomolecules
  25. Supporting Lab Techniques
  26. Toxicology

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