Current Protocols in Immunology
Online ISBN: 9780471142737
DOI: 10.1002/0471142735
Archive
Current Protocols in Immunology Supplements
2013: Supplement 101 Supplement 100
2012: Supplement 99 Supplement 98 Supplement 97 Supplement 96
2011: Supplement 95 Supplement 94 Supplement 93 Supplement 92
2010: Supplement 91 Supplement 90 Supplement 89 Supplement 88
2009: Supplement 87 Supplement 86 Supplement 85 Supplement 84
2008: Supplement 83 Supplement 82 Supplement 81 Supplement 80
2007: Supplement 79 Supplement 78 Supplement 77 Supplement 76
2006: Supplement 75 Supplement 74 Supplement 73 Supplement 72 Supplement 71
2005: Supplement 70 Supplement 69 Supplement 68 Supplement 67 Supplement 66 Supplement 65
2004: Supplement 64 Supplement 63 Supplement 62 Supplement 61 Supplement 60 Supplement 59
2003: Supplement 58 Supplement 57 Supplement 56 Supplement 55 Supplement 54 Supplement 53
2002: Supplement 52 Supplement 51 Supplement 50 Supplement 49 Supplement 48 Supplement 47
2001: Supplement 46 Supplement 45 Supplement 44 Supplement 43 Supplement 42 Supplement 41
2000: Supplement 40 Supplement 39 Supplement 38 Supplement 37 Supplement 36 Supplement 35
1999: Supplement 34 Supplement 33 Supplement 32 Supplement 31 Supplement 30 Supplement 29
1998: Supplement 28 Supplement 27 Supplement 26 Supplement 25
1997: Supplement 24 Supplement 23 Supplement 22 Supplement 21
1996: Supplement 20 Supplement 19 Supplement 18 Supplement 17
1995: Supplement 16 Supplement 15 Supplement 14 Supplement 13
1994: Supplement 12 Supplement 11 Supplement 10 Supplement 9
1993: Supplement 8 Supplement 7 Supplement 6 Supplement 5
1992: Supplement 4 Supplement 3 Supplement 2 Supplement 1
1991: Core publication
Supplement 101, April 2013
Unit 14.29 Target-Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
Abstract | Full Text: HTML PDF
Unit 14.33 Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy
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Unit 15.14 Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy
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Unit 15.23 Mouse Model for Pyelonephritis
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Supplement 100, February 2013
Unit 12.13 Evaluation of B Cell Function in Patients with HIV
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Unit 14.31 Measurement of Phagocytic Engulfment of Apoptotic Cells by Macrophages Using pHrodo Succinimidyl Ester
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Unit 14.32 Comparing Individual NK Cell Activity In Vitro
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Unit 15.8 Experimental Autoimmune Myasthenia Gravis in the Mouse
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Unit 18.3 Measurement of MHC/Peptide Interactions by Gel Filtration or Monoclonal Antibody Capture
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Supplement 99, November 2012
Unit 3.19 Isolation of Mouse Lymphocytes from Small Intestine Tissues
Abstract | Full Text: HTML PDF
Unit 7.32 Isolation and Generation of Human Dendritic Cells
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Unit 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
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Unit 14.30 Measurement of Phagocytosis, Phagosome Acidification, and Intracellular Killing of Staphylococcus aureus
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Appendix 3G Cryopreservation and Thawing of Cells
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Supplement 98, August 2012
Unit 3.24 Generation and Characterization of Mouse Basophils from Bone Marrow and Purification of Basophils from Spleen
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Unit 7.31 Isolation of Human Eosinophils
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Unit 22F.11 Differentiation and Characterization of Dendritic Cells from Human Embryonic Stem Cells
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Supplement 97, April 2012
Unit 7.40 Isolation of Cells from the Feto-Maternal Interface
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Unit 14.29 Target-Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
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Unit 18.18 Analysis of the Targeting Properties of Fc Chimeric Proteins and Antibodies in Mice In Vivo
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Unit 22F.10 Differentiation of Mast Cells from Embryonic Stem Cells
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Supplement 96, February 2012
Unit 8.17 Single-Cell Phospho-Protein Analysis by Flow Cytometry
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Unit 14.28 Analysis of Gene Expression and Gene Silencing in Human Macrophages
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Unit 15.24 Mouse Models of Atherosclerosis
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Unit 19.3 Animal Models for Toxoplasma gondii Infection
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Supplement 95, November 2011
Unit 7.21B Immortalization of Human and Rhesus Macaque Primary Antigen-Specific T Cells by Retrovirally Transduced Telomerase Reverse Transcriptase
Abstract | Full Text: HTML PDF
Unit 14.26 Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated by Phagocytes
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Unit 14.27 Measuring Opsonic Phagocytosis via Fcγ Receptors and Complement Receptors on Macrophages
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Unit 15.23 Experimental Autoimmune Myasthenia Gravis in the Mouse
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Supplement 94, August 2011
Unit 7.5 Isolation of Human B Cell Populations
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Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
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Unit 10.33 Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR
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Unit 19.5 Animal Models of Mycobacteria Infection
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Supplement 93, April 2011
Unit 11.7 Protein Tyrosine Phosphatase Assays
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Unit 14.22 Quantitative Assessment of Macrophage Functions in Repair and Fibrosis
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Unit 14.23 Measuring the Killing of Intracellular Pathogens: Leishmania
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Unit 14.24 Measuring Matrix Metalloproteinase Activity in Macrophages and Polymorphonuclear Leukocytes
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Unit 14.25 Measurement of Macrophage-Mediated Killing of Intracellular Bacteria, Including Francisella and Mycobacteria
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Unit 19.4 Experimental Asexual Blood Stage Malaria Immunity
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Unit 19.14 Infection of Mice with Francisella as an Immunological Model
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Supplement 92, February 2011
Unit 14.18 Measurement of Tumor Cytolysis by Macrophages
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Unit 14.19 In Vivo and Ex Vivo Protocols for Measuring the Killing of Extracellular Pathogens by Macrophages
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Unit 14.20 Measurement of Macrophage Growth and Differentiation
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Unit 14.21 Measurement of Type I Interferon Production
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Supplement 91, November 2010
Unit 7.38 Measuring Mast Cell Mediator Release
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Unit 7.39 Evaluation of Human Natural Killer Cell Activities in Whole Blood
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Unit 10.17E Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models
Abstract | Full Text: HTML PDF
Unit 14.16 Measuring TLR Function in Transfectants
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Unit 14.17 Measurement of Myeloid Cell Immune Suppressive Activity
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Unit 19.13 Cecal Ligation and Puncture
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Supplement 90, August 2010
Unit 7.25 Isolation of Tissue Mast Cells
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Unit 7.37 Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines Derived from Peripheral Blood or Cord Blood
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Unit 9.9 Epitope Mapping Using Gram-Positive Surface Display
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Unit 11.9B Signal Transduction During Activation and Inhibition of Natural Killer Cells
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Unit 14.11 Isolation and Functional Use of Human NKT Cells
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Supplement 89, April 2010
Unit 2.16 Efficient Delivery of Antibody Into Living Cells Using Hemagglutinating Virus of Japan (HVJ) Envelope
Abstract | Full Text: HTML PDF
Unit 8.15 Endoglycosidase and Glycoamidase Release of N-Linked Glycan
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Unit 10.17D Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro
Abstract | Full Text: HTML PDF
Unit 11.3 Detecting Tyrosine-Phosphorylated Proteins by Western Blot Analysis
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Unit 15.5 Collagen-Induced Arthritis
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Supplement 88, February 2010
Unit 14.15 Monocyte and Neutrophil Isolation and Migration Assays
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Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
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Unit 16.1 Choosing and Preparing Antigen-Presenting Cells
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Unit 16.2 Presenting Exogenous Antigens to T Cells
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Unit 18.17 The MHC Motif Viewer: A Visualization Tool for MHC Binding Motifs
Abstract | Full Text: HTML PDF
Supplement 87, November 2009
Unit 5.9 Visualization of Multiprotein Complexes by Flow Cytometry
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Unit 11.1 Phosphoinositide and Inositol Phosphate Analysis in Lymphocyte Activation
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Unit 11.9D TRAF-Mediated TNFR-Family Signaling
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Unit 14.14 Measuring Autophagy in Macrophages
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Unit 17.5 Preparation of Stable Single-Chain Trimers Engineered with Peptide, β2 Microglobulin, and MHC Heavy Chain
Abstract | Full Text: HTML PDF
Unit 18.16 Generation of Peptide MHC Class I Monomers and Multimers Through Ligand Exchange
Abstract | Full Text: HTML PDF
Supplement 86, August 2009
Unit 3.7 Isolation of Dendritic Cells
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Unit 7.8 Isolation of Mononuclear Cells from Tonsillar Tissue
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Unit 9.8 Phage Display Selection, Analysis, and Prediction of B Cell Epitopes
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Supplement 85, April 2009
Unit 2.9 Isolation of Murine and Human Immunoglobin M and Murine Immunoglobin D
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Unit 2.9B Measurement of Human Serum IgD Levels
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Unit 2.15 Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins
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Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
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Unit 7.4 Immunomagnetic Purification of T Cell Subpopulations
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Unit 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
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Unit 20.11 The Immune Response to Tumors
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Supplement 84, February 2009
Unit 4.4 Skin Allograft Rejection
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Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
Abstract | Full Text: HTML PDF
Unit 14.10 Human Natural Killers Receptors, Co-Receptors, and Their Ligands
Abstract | Full Text: HTML PDF
Unit 20.10 Molecular Cloning and Characterization of MHC Class I- and II-Restricted Tumor Antigens Recognized by T Cells
Abstract | Full Text: HTML PDF
Supplement 83, November 2008
Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
Abstract | Full Text: HTML PDF
Unit 8.10 Immunoblotting and Immunodetection
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Unit 8.10B Detection of Proteins on Blot Transfer Membranes
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Unit 8.15 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
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Unit 14.1 The Isolation and Characterization of Murine Macrophages
Abstract | Full Text: HTML PDF
Unit 14.2 Activation of Murine Macrophages
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Supplement 82, August 2008
Unit 1.12 Techniques in Aseptic Rodent Surgery
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Unit 2.4 Production of Polyclonal Antisera
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Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
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Unit 20.9 ErbB2 Transgenic Mice: A Tool for Investigation of the Immune Prevention and Treatment of Mammary Carcinomas
Abstract | Full Text: HTML PDF
Supplement 81, May 2008
Unit 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras
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Unit 15.21 Creation of "Humanized" Mice to Study Human Immunity
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Unit 15.22 The K/BxN Arthritis Model
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Unit 18.15 Characterizing Protein-Protein Interactions by Sedimentation Velocity Analytical Ultracentifrugation
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Appendix 1X Paired Receptor Systems of the Innate Immune System
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Supplement 80, February 2008
Unit 20.8 Carcinoembryonic Antigen Transgenic Mouse Models for Immunotherapy and Development of Cancer Vaccines
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Unit 22B.2 Characterization of Mouse Hematopoietic Stem and Progenitor Cells
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Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
Abstract | Full Text: HTML PDF
Supplement 79, November 2007
Unit 6.25 Measurement of Interleukin-17
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Unit 15.18 Animal Models of Airway Sensitization
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Unit 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
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Unit 22A.2 Sources of Human and Murine Hematopoietic Stem Cells
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Supplement 78, August 2007
Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
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Unit 6.30 Measurement of Interleukin-21
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Unit 8.17 Single-Cell Phospho-Protein Analysis by Flow Cytometry
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Unit 15.7 Experimental Autoimmune Thyroiditis in the Mouse
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Supplement 77, May 2007
Unit 1.2 Managing Immunocompromised Animals
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Unit 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
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Unit 10.32 Use of Bacterial Artificial Chromosomes in Generating Targeted Mutations in Human and Mouse Cytomegaloviruses
Abstract | Full Text: HTML PDF
Unit 14.12 Toll-Like Receptors
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Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
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Unit 16.3 Targeted Identification of Infection-Related HLA Class I–Presented Epitopes by Stable Isotope Tagging of Epitopes (SITE)
Abstract | Full Text: HTML PDF
Unit 18.14 Measurement of Peptide Dissociation from MHC Class II Molecules
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Supplement 76, February 2007
Unit 1.1 Animal Health Assurance
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Unit 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
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Unit 8.7 Detection of Protein-Protein Interactions by Coprecipitation
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Unit 18.13 Supported Planar Bilayers for Study of the Immunological Synapse
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Supplement 75, November 2006
Unit 7.5 Isolation of Human B Cell Populations
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Unit 8.4 One-Dimensional SDS Gel Electrophoresis of Proteins
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Unit 22F.9 Differentiation of Multipotent Adult Progenitor Cells into Functional Endothelial and Smooth Muscle Cells
Abstract | Full Text: HTML PDF
Supplement 74, August 2006
Unit 2.5 Production of Monoclonal Antibodies
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Unit 3.23 Generation, Isolation, and Maintenance of Rodent Mast Cells and Mast Cell Lines
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Appendix 1Q Safe Use of Radioisotopes
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Supplement 73, June 2006
Unit 1.3 Handling and Restraint
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Unit 1.5 Animal Identification
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Unit 1.6 Parenteral Injections
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Unit 1.7 Blood Collection
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Unit 1.8 Euthanasia
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Unit 14.13 Characterization and Functional Analysis of Mouse Invariant Natural T (iNKT) Cells
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Supplement 72, April 2006
Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
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Unit 3.13 Production of T H 1 and T H 2 Cell Lines and Clones
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Unit 7.4B The Purification and Functional Analysis of Human CD4 CD25 high Regulatory T Cells
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Supplement 71, February 2006
Unit 3.18 In Vitro Systems for the Study of T Cell Development: Fetal Thymus Organ Culture and OP9-DL1 Cell Coculture
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Appendix 1J Web Sites of Interest to Immunologists
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Appendix 1W Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors
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Supplement 70, December 2005
Unit 7.6A Isolation of Human Monocyte Populations
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Unit 7.6B Isolation and Purification of Human Intestinal Macrophages
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Unit 12.4 Culture of HIV in Monocytes and Macrophages
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Unit 12.5 Detection Assays for HIV Proteins
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Supplement 69, October 2005
Unit 12.3 Infection of CD4 Primary T Cells and Cell Lines, Generation of Chronically Infected Cell Lines, and Induction of HIV Expression
Abstract | Full Text: HTML PDF
Unit 22A.1 Large Animal Models for Stem and Progenitor Cell Analysis
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Unit 22F.7 Differentiation of Human Erythroid Cells in Culture
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Unit 22F.8 Differentiation and Characterization of T Cells
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Appendix 1V Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
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Supplement 68, August 2005
Unit 8.5 Two-Dimensional Gel Electrophoresis
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Unit 13.8 Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
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Unit 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
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Supplement 67, June 2005
Unit 13.7 Assays for Complement Proteins Encoded in the Class III Region of Human MHC
Abstract | Full Text: HTML PDF
Unit 22B.1 Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells
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Unit 22F.4 Differentiation of Peripheral Blood Monocytes into Dendritic Cells
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Unit 22F.5 Differentiation and Characterization of Myeloid Cells
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Unit 22F.6 Culture, Expansion, and Differentiation of Murine Megakaryocytes
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Supplement 66, April 2005
Unit 7.18B Measurement of Cytokine Production Using Whole Blood
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Unit 22A.1 Sources and Methods for Obtaining Stem and Progenitor Cells
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Unit 22F.1 Isolation of Prolymphocytes from Bone Marrow and Fetal Liver
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Unit 22F.2 In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture
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Unit 22F.3 Measurement of Natural Killer Cell Progenitor Activity in Culture
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Supplement 65, February 2005
Unit 7.36 In Vitro Models of Human T Cell Anergy
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Unit 12.12 Immunophenotyping of T Cell Subpopulations in HIV Disease
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Unit 20.7 Identification of Human Tumor Antigens by Serological Analysis of Recombinant cDNA Expression Libraries (SEREX)
Abstract | Full Text: HTML PDF
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Supplement 64, December 2004
Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
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Unit 12.11 Evaluating Neutralizing Antibodies Against HIV, SIV, and SHIV in Luciferase Reporter Gene Assays
Abstract | Full Text: HTML PDF
Unit 17.4 In Situ Staining Using MHC Class I Tetramers
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Supplement 63, October 2004
Unit 7.21 Herpesvirus saimiri Transformation of Human T Lymphocytes
Abstract | Full Text: HTML PDF
Unit 7.35 Assays for B Cell and Germinal Center Development
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Unit 10.31 Measurement of Mouse T Cell Receptor Excision Circles
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Supplement 62, August 2004
Unit 10.30 Analysis of Telomere Length and Telomerase Activity
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Unit 12.4 Culture of HIV in Monocytes and Macrophages
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Supplement 61, June 2004
Unit 6.29 Cytokine, Growth Factor, and Chemokine Ligand Database
Abstract | Full Text: HTML PDF
Unit 18.12 Competition-Based Cellular Peptide Binding Assay for HLA Class I
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Supplement 60, April 2004
Unit 3.12 Proliferative Assays for T Cell Function
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Unit 5.7 Analysis of Cellular DNA Content by Flow Cytometry
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Unit 7.34 Isolation and Characterization of Human Natural Killer Cell Subsets
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Unit 9.4 B Cell Epitope Mapping Using Synthetic Peptides
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Supplement 59, February 2004
Unit 3.17 Measurement of Apoptosis and Other Forms of Cell Death
Abstract | Full Text: HTML PDF
Unit 12.1 General Guidelines for Experimenting with HIV
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Appendix 1U Large-Scale Cell Culture
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Supplement 58, December 2003
Unit 8.9 Staining Proteins in Gels
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Unit 11.8 Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells
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Unit 13.6 Assays for the Mannan-Binding Lectin Pathway
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Appendix 1T Heat-Shock Proteins
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Supplement 57, October 2003
Unit 3.9 Early Events in B Lymphocyte Activation
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Unit 8.1A Solubilization of Lymphocytes
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Unit 8.1C Subcellular Fractionation of Tissue Culture Cells
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Unit 18.11 Peptide Induction of Surface Expression of Class I MHC
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Supplement 56, August 2003
Unit 3.8 Assays for B Lymphocyte Function
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Unit 8.1B Purification of Organelles from Mammalian Cells
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Supplement 55, June 2003
Unit 3.5A Fractionation of T and B Cells Using Magnetic Beads
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Unit 9.2 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
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Unit 9.3 Production of Anti-Peptide Antisera
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Unit 19.12 Animal Model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus
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Supplement 54, April 2003
Unit 6.28 The In Vivo Cytokine Capture Assay for Measurement of Cytokine Production in the Mouse
Abstract | Full Text: HTML PDF
Unit 10.29 Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression
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Unit 12.7 Regulation of the HIV Promoter/Enhancer
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Unit 12.10 Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors
Abstract | Full Text: HTML PDF
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Supplement 53, February 2003
Unit 15.6 Experimental Autoimmune Uveoretinitis in the Rat and Mouse
Abstract | Full Text: HTML PDF
Unit 17.3 MHC-Peptide Tetramers to Visualize Antigen-Specific T Cells
Abstract | Full Text: HTML PDF
Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
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Supplement 52, December 2002
Unit 14.11 Isolation and Functional Use of Human NK T Cells
Abstract | Full Text: HTML PDF
Unit 14.12 Toll Receptors
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Unit 15.20 Animal Models for SLE
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Unit 18.10 Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation and Molecular Proximity
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Appendix 1S Nomenclature and Serology of HLA Class I and Class II Alleles
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Supplement 51, October 2002
Unit 6.13 Measurement of Mouse and Human Interleukin 9
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Unit 11.9D TRAF-Mediated TNFR-Family Signaling
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Unit 11.10 Isolation and Use of Rafts
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Appendix 1R Receptors Involved in the Regulation of Murine NK Cells
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Supplement 50, August 2002
Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
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Unit 9.8 Phage Display Selection and Analysis of Ab-Binding Epitopes
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Unit 18.9 Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance
Abstract | Full Text: HTML PDF
Appendix 3J Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
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Supplement 49, June 2002
Unit 5.8 Multiparameter FACS Analysis
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Unit 7.5 Isolation of B Cell Populations
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Unit 9.2 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
Unit 21.4 Immunohistochemistry
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Appendix 1M Listing, Location, Binding Motifs, and Expression of Nonclassical Class I and Related Genes and Molecules
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Supplement 48, April 2002
Unit 10.19A Construction of Immunoglobulin Fusion Proteins
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Unit 10.19B Phage Display of Single-Chain Antibody Constructs
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Unit 21.1 Proper Alignment and Adjustment of the Light Microscope
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Unit 21.2 Fluorescence Microscopy
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Unit 21.3 Immunofluorescence Staining
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Supplement 47, February 2002
Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions
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Unit 7.19 Generation and Maintenance of Cloned Human T Cell Lines
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Unit 7.20 Generation of HTLV-I-Transformed T Cell Lines
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Unit 9.1 Introduction to Peptide Synthesis
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Appendix 4B Monoclonal Antibodies to Mouse Cell-Surface Antigens
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Supplement 46, December 2001
Unit 6.18 Measurement of Interleukin-13
Abstract | Full Text: HTML PDF
Unit 6.27 Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay
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Unit 8.9 Staining Proteins in Gels
Unit 14.10 Human Natural Killer Receptors and Their Ligands
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Unit 20.6 Models for Lymphoma
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Supplement 45, October 2001
Unit 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
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Unit 15.19 Induction of TNBS Colitis in Mice
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Unit 20.5 The TRAMP Mouse as a Model for Prostate Cancer
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Supplement 44, August 2001
Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
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Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACS Calibur
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Unit 6.26 Measurement of Human and Mouse Interleukin 18
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Unit 11.9C Apoptosis Signaling Pathways
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Supplement 43, June 2001
Unit 1.11 Surgical Removal of Mouse Salivary Glands
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Unit 20.4 The P815 Mastocytoma Tumor Model
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Supplement 42, April 2001
Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
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Unit 12.4 Culture of HIV in Monocytes and Macrophages
Unit 12.5 Detection Assays for HIV Proteins
Full Text: HTML PDF
Unit 19.11 Influenza Virus
Abstract | Full Text: HTML PDF
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Supplement 41, February 2001
Unit 6.11 Measurement of TGF-β in Biological Fluids
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Unit 6.25 Measurement of Interleukin 17
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Unit 8.3 Immunoprecipitation
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Appendix 1Q Safe Use of Radioisotopes
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Supplement 40, December 2000
Unit 2.6 Monoclonal Antibody Supernatant and Ascites Fluid Production
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Unit 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
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Appendix 1O Nomenclature of the Human T Cell Receptor Genes
Abstract | Full Text: HTML PDF
Appendix 1P Nomenclature of the Human Immunoglobulin Genes
Abstract | Full Text: HTML PDF
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Supplement 39, October 2000
Unit 9.4 Production of Anipeptide Antisera
Unit 20.1 B16 as a Mouse Model for Human Melanoma
Abstract | Full Text: HTML PDF
Unit 20.2 Mouse 4T1 Breast Tumor Model
Abstract | Full Text: HTML PDF
Unit 20.3 Mouse SaI Sarcoma Tumor Model
Abstract | Full Text: HTML PDF
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Supplement 38, August 2000
Unit 2.10A Fragmentation of Immunoglobulin M
Abstract | Full Text: HTML PDF
Unit 2.10B Purification of Human IgA
Abstract | Full Text: HTML PDF
Unit 6.12 Biological Responses to Chemokine Superfamily Members
Abstract | Full Text: HTML PDF
Unit 10.28 Spectratype/Immunoscope Analysis of the Expressed TCR Repertoire
Abstract | Full Text: HTML PDF
Unit 12.6 Detection of HIV DNA and RNA Using PCR
Abstract | Full Text: HTML PDF
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Supplement 37, June 2000
Unit 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay
Abstract | Full Text: HTML PDF
Unit 11.10 Isolation and Use of Rafts
Appendix 1N Overview of Protein Folds in the Immune System
Abstract | Full Text: HTML PDF
Supplement 36, April 2000
Unit 18.7 Biotin Labeling and Quantitation of Cell-Surface Proteins
Abstract | Full Text: HTML PDF
Unit 19.8 Helicobacter Animal Models
Abstract | Full Text: HTML PDF
Unit 19.9 Animal Model for Infection with Listeria monocytogenes
Abstract | Full Text: HTML PDF
Unit 19.10 Animal Models Using Lymphocytic Choriomeningitis Virus
Abstract | Full Text: HTML PDF
Appendix 1I Peptide Binding Motifs for MHC Class I and II Molecules
Abstract | Full Text: HTML PDF
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Supplement 35, February 2000
Unit 11.9A Key Molecules Involved in Receptor-Mediated Lymphocyte Activation
Abstract | Full Text: HTML PDF
Unit 11.9B Signal Transduction During Natural Killer Cell Activation
Abstract | Full Text: HTML PDF
Unit 17.2 Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers
Abstract | Full Text: HTML PDF
Appendix 1J Web Sites of Interest to Immunologists
Full Text: HTML PDF
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Supplement 34, December 1999
Unit 9.6 Synthesis and Application of Peptide Dendrimers As Protein Mimetics
Abstract | Full Text: HTML PDF
Unit 15.18 Animal Models of Airway Sensitization
Full Text: HTML PDF
Unit 17.1 Bacteriophage Library Construction and Selection of Recombinant Antibodies
Abstract | Full Text: HTML PDF
Appendix 2A Common Media, Buffers, and Stock Solutions
Abstract | Full Text: HTML PDF
Appendix 2B Equipment
Abstract | Full Text: HTML PDF
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Supplement 33, October 1999
Unit 7.33 Measurement of Prostaglandins and Other Eicosanoids
Abstract | Full Text: HTML PDF
Unit 18.5 Measurement of the Kinetics of Biomolecular Interactions Using the IAsys Resonant Mirror Biosensor
Abstract | Full Text: HTML PDF
Unit 18.6 Affinity-Based Biosensors for Biomolecular Interaction Analysis
Abstract | Full Text: HTML PDF
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Supplement 32, August 1999
Unit 15.15 Mercuric Chloride–Induced Autoimmunity
Abstract | Full Text: HTML PDF
Unit 15.16 The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases
Abstract | Full Text: HTML PDF
Unit 15.17 Autoimmune Ovarian Disease Induced by Immunization with Zona Pellucida (ZP3) Peptide
Abstract | Full Text: HTML PDF
Unit 19.7 A Mouse Model for Cytomegalovirus Infection
Abstract | Full Text: HTML PDF
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Supplement 31, June 1999
Unit 3.22 Isolation of Murine Natural Killer Cells
Abstract | Full Text: HTML PDF
Unit 10.17C Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T Cell–Based Systems
Abstract | Full Text: HTML PDF
Unit 15.14 Autoimmune Myocarditis
Abstract | Full Text: HTML PDF
Unit 18.4 Measurement of Peptide-MHC Interactions in Solution Using the Spin Column Filtration Assay
Abstract | Full Text: HTML PDF
Appendix 4C Monoclonal Antibodies to Rat Leukocyte Surface Antigens, MHC Antigens, and Immunoglobulins
Abstract | Full Text: HTML PDF
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Supplement 30, April 1999
Unit 15.12 Induction of Autoimmune Disease by Depletion of Regulatory T Cells
Abstract | Full Text: HTML PDF
Unit 15.13 Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Depletion of Regulatory T Cells
Abstract | Full Text: HTML PDF
Unit 19.5 Animal Models of Mycobacteria Infection
Abstract | Full Text: HTML PDF
Unit 19.6 Animal Models for Candidiasis
Abstract | Full Text: HTML PDF
Appendix 1G HLA Type of EBV-Transformed Human B Cell Lines
Abstract |Full Text: HTML PDF
Supplement 29, February 1999
Unit 15.11 Animal Models of IgA Nephropathy
Abstract | Full Text: HTML PDF
Unit 19.4 Experimental Asexual Blood Stage Malaria Immunity
Abstract | Full Text: HTML PDF
Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
Supplement 28, December 1998
Unit 11.8 Measurement of Mitogen-Activated Protein Kinase (MAPK) in Activated T Cells
Unit 19.1 Schistosomiasis
Abstract | Full Text: HTML PDF
Unit 19.2 Animal Models for the Analysis of Immune Responses to Leishmaniasis
Abstract | Full Text: HTML PDF
Unit 19.3 Animal Models for Toxoplasma gondii Infection
Abstract | Full Text: HTML PDF
Appendix 1M Apoptosis Signaling Pathways
Supplement 27, September 1998
Unit 1.4 Anesthesia
Abstract | Full Text: HTML PDF
Unit 2.14 Nucleic Acid Immunizations
Abstract | Full Text: HTML PDF
Unit 4.3 Animal Models of Acute and Chronic Graft-Versus-Host Disease
Abstract | Full Text: HTML PDF
Unit 10.3 Preparation of Bacterial Plasmid DNA
Abstract | Full Text: HTML PDF
Appendix 1K Human MHC Genes and Products
Abstract | Full Text: HTML PDF
Appendix 1L Mouse MHC Genes and Products
Abstract | Full Text: HTML PDF
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Supplement 26, June 1998
Unit 6.21 Detection of Cytokine Receptors by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
Unit 8.10 Immunoblotting and Immunodetection
Full Text: HTML PDF
Unit 15.10 Streptococcal Cell Wall Arthritis
Abstract | Full Text: HTML PDF
Unit 18.2 Detection of Low-Affinity Ligand-Receptor Interactions at the Cell Surface with Fluorescent Microspheres
Abstract | Full Text: HTML PDF
Unit 18.3 Measurement of MHC/Peptide Interactions by Gel Filtration
Abstract | Full Text: HTML PDF
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Supplement 25, March, 1998
Unit 3.7 Isolation of Dendritic Cells
Full Text: HTML PDF
Unit 3.16 Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation
Abstract | Full Text: HTML PDF
Unit 3.17 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis
Unit 4.8 Construction of Human-SCID Chimeric Mice
Abstract | Full Text: HTML PDF
Unit 18.1 Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces
Abstract | Full Text: HTML PDF
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Supplement 24, December 1997
Unit 3.13 Production of T Cell Clones
Full Text: HTML PDF
Unit 3.14 Production of Mouse T Cell Hybridomas
Abstract | Full Text: HTML PDF
Unit 10.20 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Abstract | Full Text: HTML PDF
Unit 10.21 Quantitation of Rare DNAs by PCR
Abstract | Full Text: HTML PDF
Unit 15.9 The NOD Mouse: A Model for Insulin-Dependent Diabetes Mellitus
Abstract | Full Text: HTML PDF
Unit 17.1 Bacteriophage Library Construction and Selection of Recombinant Antibodies
Supplement 23, September 1997
Unit 6.1 Measurement of Lymphokine Receptors
Abstract | Full Text: HTML PDF
Unit 7.32 Isolation and Generation of Human Dendritic Cells
Abstract | Full Text: HTML PDF
Unit 16.1 Choosing and Preparing Antigen-Presenting Cells
Full Text: HTML PDF
Unit 16.2 Presenting Exogenous Antigen to T Cells
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Appendix 1J Key Molecules Involved in Receptor-Mediated Lymphocyte Activation
Appendix 3O Gram Staining
Abstract | Full Text: HTML PDF
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Supplement 22, June 1997
Unit 3.20 Isolation of Mouse Neutrophils
Abstract | Full Text: HTML PDF
Unit 3.21 Isolation of Mouse Intrahepatic Lymphocytes
Abstract | Full Text: HTML PDF
Unit 6.23 Measurement of Interleukin 16
Abstract | Full Text: HTML PDF
Unit 9.7 Synthesis of Multiple Peptides on Plastic Pins
Abstract | Full Text: HTML PDF
Unit 10.27 Molecular Analysis of Mouse T Cell Receptor Expression Using PCR
Abstract | Full Text: HTML PDF
Unit 11.7 Using Protein Kinase and Protein Phosphatase Inhibitors to Dissect Protein Phosphorylation Pathways
Abstract | Full Text: HTML PDF
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Supplement 21, March 1997
Unit 2.7 Purification of Immunoglobulin G
Abstract | Full Text: HTML PDF
Unit 2.8 Fragmentation of Immunoglobulin G
Abstract | Full Text: HTML PDF
Unit 2.9 Purification of Immunoglobulin M and Immunoglobulin D
Full Text: HTML PDF
Unit 2.10 Fragmentation of Immunoglobin M
Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
Full Text: HTML PDF
Unit 15.8 Experimental Autoimmune Myasthenia Gravis in the Mouse
Abstract | Full Text: HTML PDF
Appendix 3A Monitoring Cell Growth
Abstract | Full Text: HTML PDF
Appendix 3B Trypan Blue Exclusion Test of Cell Viability
Abstract | Full Text: HTML PDF
Appendix 3C Wright-Giemsa and Nonspecific Esterase Staining of Cells
Abstract | Full Text: HTML PDF
Appendix 3D Labeling Cells in Microtiter Plates for Determination of [ 3 H]Thymidine Uptake
Abstract | Full Text: HTML PDF
Appendix 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
Abstract | Full Text: HTML PDF
Appendix 3F Obtaining Human Peripheral Blood Cells
Abstract | Full Text: HTML PDF
Appendix 3G Cryopreservation of Cells
Abstract | Full Text: HTML PDF
Appendix 3H Dialysis and Concentration of Protein Solutions
Abstract | Full Text: HTML PDF
Appendix 3I Column Chromatography of Antibodies and Fragments
Abstract |Full Text: HTML PDF
Appendix 3K Silanizing Glassware
Abstract |Full Text: HTML PDF
Appendix 3L Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract |Full Text: HTML PDF
Appendix 3M Preparing Frozen Bacterial Stocks
Abstract |Full Text: HTML PDF
Appendix 3N Transformation of E. coli by Electroporation
Abstract |Full Text: HTML PDF
Supplement 20, December 1996
Unit 5.8 Immunohistochemistry
Unit 7.31 Isolation of Human Eosinophils
Abstract | Full Text: HTML PDF
Unit 11.6 Production of Antibodies that Recognize Specific Tyrosine-Phosphorylated Peptides
Unit 15.5 Collagen-Induced Arthritis
Full Text: HTML PDF
Unit 15.6 Experimental Autoimmune Uveoretinitis in the Rat and Mouse
Unit 15.7 Experimental Autoimmune Thyroiditis in the Mouse
Full Text: HTML PDF
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Supplement 19, September 1996
Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
Full Text: HTML PDF
Unit 7.30 Isolation of Human Intestinal Mucosal Mononuclear Cells
Abstract | Full Text: HTML PDF
Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
Full Text: HTML PDF
Unit 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
Full Text: HTML PDF
Unit 15.3 The BB Rat as a Model of Human Insulin-Dependent Diabetes Mellitus
Abstract | Full Text: HTML PDF
Unit 15.4 Adjuvant Arthritis in the Rat
Abstract | Full Text: HTML PDF
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Supplement 18, June 1996
Unit 2.9 Purification of Immunoglobulin M and Immunoglobulin D
Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF, Erythropoietin, Steel Factor, and Flt-3 Ligand
Abstract | Full Text: HTML PDF
Unit 6.13 Measurement of Mouse and Human Interleukin 9
Unit 6.14 Measurement of Mouse and Human Interleukin 10
Abstract | Full Text: HTML PDF
Unit 6.15 Measurement of Human Interleukin 11
Abstract | Full Text: HTML PDF
Unit 6.22 Measurement of Interleukin 15
Abstract | Full Text: HTML PDF
Unit 8.2 Immunoaffinity Chromatography
Abstract | Full Text: HTML PDF
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Supplement 17, March 1996
Unit 6.6 Measurement of Interleukin 6
Abstract | Full Text: HTML PDF
Unit 6.7 Measurement of Interleukin 7
Abstract | Full Text: HTML PDF
Unit 7.7 Isolation of Human NK Cells and Generation of LAK Activity
Abstract | Full Text: HTML PDF
Unit 7.14 Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
Abstract | Full Text: HTML PDF
Unit 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
Abstract | Full Text: HTML PDF
Unit 7.18 Measurement of Cytotoxic Activity of NK/LAK Cells
Abstract | Full Text: HTML PDF
Appendix 1A Abbreviations Used in this Manual
Abstract | Full Text: HTML PDF
Appendix 1B Amino Acids
Abstract | Full Text: HTML PDF
Appendix 1C Commonly Used Mouse Strains
Abstract | Full Text: HTML PDF
Appendix 1D Commonly Used Detergents
Abstract | Full Text: HTML PDF
Appendix 1E Murine Models of Immunodeficiency and Autoimmune Disease
Abstract | Full Text: HTML PDF
Appendix 1F Mouse Endogenous Superantigens: Ms and Mls-like Determinants Encoded by Mouse Retroviruses
Abstract | Full Text: HTML PDF
Appendix 1G HLA Type of EBV-Transformed Human B Cell Lines
Appendix 1H Commonly Used Rat Strains
Abstract | Full Text: HTML PDF
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Supplement 16, December 1995
Unit 2.11 Preparation of Immune Stimulating Complexes (ISCOMs) as Adjuvants
Abstract | Full Text: HTML PDF
Unit 3.17 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis
Unit 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
Full Text: HTML PDF
Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan
Unit 7.5 Isolation of B Cell Populations
Appendix 1I Peptide Binding Motifs for MHC Class I and II Molecules
Supplement 15, September 1995
Unit 1.7 Blood Collection
Full Text: HTML PDF
Unit 1.8 Euthanasia
Full Text: HTML PDF
Unit 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes, Peyer's Patch, and Lamina Propria Cells
Abstract | Full Text: HTML PDF
Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4
Abstract | Full Text: HTML PDF
Unit 6.16 Measurement of Human and Mouse Interleukin-12
Abstract | Full Text: HTML PDF
Unit 6.21 Detection of Cytokine Receptors by Flow Cytometry
Unit 7.29 Cell Adhesion Under Hydrodynamic Flow Conditions
Abstract | Full Text: HTML PDF
Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
Supplement 14, June 1995
Unit 1.1 Managing Immunocompromised Animals
Full Text: HTML PDF
Unit 1.2 Managing Immunocompromised Animals
Full Text: HTML PDF
Unit 1.3 Handling and Restraint
Full Text: HTML PDF
Unit 1.4 Anesthesia
Unit 1.5 Animal Identification
Full Text: HTML PDF
Unit 1.6 Parenteral Injections
Full Text: HTML PDF
Unit 2.13 Production of Bispecific Antibodies
Abstract | Full Text: HTML PDF
Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
Full Text: HTML PDF
Unit 10.26 Evaluating Human T Cell Receptor Gene Expression by PCR
Abstract | Full Text: HTML PDF
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Supplement 13, March 1995
Unit 2.4 Production of Polyclonal Antisera
Full Text: HTML PDF
Unit 6.12 Measurement of α and β Chemokines
Unit 6.20 Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies
Abstract | Full Text: HTML PDF
Unit 8.16 Biotinylation and Analysis of Membrane-Bound and Soluble Proteins
Abstract | Full Text: HTML PDF
Unit 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
Full Text: HTML PDF
Unit 14.9 Measurement of Interferon-Mediated Antiviral Activity of Macrophages
Abstract | Full Text: HTML PDF
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Supplement 12, December 1994
Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions
Unit 10.17A Stable Transfer of Genes into Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 10.17B Transfection of Lymphoid Cells
Abstract | Full Text: HTML PDF
Unit 10.25 Double-Stranded DNA Sequence Analysis
Abstract | Full Text: HTML PDF
Unit 14.5 Oxidative Metabolism of Murine Macrophages
Abstract | Full Text: HTML PDF
Unit 14.6 Measurement of Bacterial Ingestion and Killing by Macrophages
Abstract | Full Text: HTML PDF
Unit 14.7 Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity
Abstract | Full Text: HTML PDF
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Supplement 11, September 1994
Unit 7.28 Measurement of Cellular Adhesion Under Static Conditions
Abstract | Full Text: HTML PDF
Unit 13.5 Isolation and Functional Assay of the Membrane Complement Inhibitors CD55 (DAF) and CD59 (MIRL)
Abstract | Full Text: HTML PDF
Unit 14.1 Isolation of Murine Macrophages
Full Text: HTML PDF
Unit 14.2 Characterization of Murine Macrophages
Full Text: HTML PDF
Unit 14.3 Characterization of Human Monocytes/Macrophages
Abstract | Full Text: HTML PDF
Unit 14.4 Activation of Murine Macrophages
Abstract | Full Text: HTML PDF
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Supplement 10, June 1994
Unit 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras
Full Text: HTML PDF
Unit 6.18 Measurement of IL-13
Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
Full Text: HTML PDF
Unit 9.7 Synthesis of Peptides Immobilized on Polypropylene Pins
Unit 9.8 Use of Synthetic Peptide Combinatorial Libraries
Unit 13.3 Isolation and Purification of C3 from Human Plasma
Abstract | Full Text: HTML PDF
Unit 13.4 Assays for Membrane Complement Receptors
Abstract | Full Text: HTML PDF
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Supplement 9, March 1994
Unit 8.13 Metabolic Radiolabeling of Animal Cell Glycoconjugates
Abstract | Full Text: HTML PDF
Unit 8.14 Inhibition of N-Linked Glycosylation
Abstract | Full Text: HTML PDF
Unit 8.15 Release of Saccharides from Glycoconjugates
Full Text: HTML PDF
Unit 13.1 Classical Pathway Evaluation
Abstract | Full Text: HTML PDF
Unit 13.2 Alternative Pathway Evaluation
Abstract | Full Text: HTML PDF
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Supplement 8, December 1993
Unit 3.5B Isolation of Ly-1/CD5 B Cells by Cell Sorting
Unit 7.26 Measuring Degranulation of Mast Cells
Abstract | Full Text: HTML PDF
Unit 7.27 51 Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
Abstract | Full Text: HTML PDF
Unit 10.1 Purification and Concentration of DNA from Aqueous Solutions
Abstract | Full Text: HTML PDF
Unit 10.5 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
Abstract | Full Text: HTML PDF
Unit 10.24 cDNA Amplification Using One-Sided (Anchored) PCR
Abstract | Full Text: HTML PDF
Unit 12.9 In Vitro Evaluation of Experimental Agents for Anti-HIV Activity
Abstract | Full Text: HTML PDF
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Supplement 7, September 1993
Unit 9.6 Synthesis and Use of MAP Peptides
Unit 10.12 Analysis of RNA by Northern and Slot-Blot Hybridization
Abstract | Full Text: HTML PDF
Unit 11.4 Immune-Complex Assays for Tyrosine Protein Kinases
Abstract | Full Text: HTML PDF
Unit 11.5 Assessment of the Effects of Tyrosine Protein Kinase Inhibitors
Abstract | Full Text: HTML PDF
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Supplement 6, June 1993
Unit 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes
Unit 4.5 Delayed-Type Hypersensitivity
Abstract | Full Text: HTML PDF
Unit 7.23 Isolation and Functional Analysis of Neutrophils
Abstract | Full Text: HTML PDF
Unit 7.24 Isolation of Human Basophils
Abstract | Full Text: HTML PDF
Unit 8.10 Immunoblotting and Immunodetection
Unit 10.6A Southern Blotting
Abstract | Full Text: HTML PDF
Unit 10.6B Hybridization Analysis of DNA Blots
Abstract | Full Text: HTML PDF
Unit 12.8 In Situ Hybridization for Detection of HIV RNA
Abstract | Full Text: HTML PDF
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Supplement 5, March 1993
Unit 12.1 General Guidelines for Experimenting with HIV
Unit 12.2 Isolation and Quantitation of HIV in Peripheral Blood
Abstract | Full Text: HTML PDF
Unit 12.3 Infection of CD4 Primary Cell Lines and Induction of HIV
Full Text: HTML
Unit 12.4 Culture of HIV in Monocytes and Macrophages
Unit 12.5 Detection Assays for HIV Proteins
Unit 12.6 Detection of HIV DNA and RNA Using PCR
Unit 12.7 Regulation of the HIV Promoter/Enhancer
Supplement 4, December 1992
Unit 2.11 Preparation of Immune Stimulating Complexes (ISCOMs) as Adjuvants
Unit 3.18 Fetal Thymus Organ Culture for T Cell Development Studies
Full Text: HTML PDF
Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan
Unit 6.16 Measurement of Human and Mouse Interleukin 12
Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand)
Abstract | Full Text: HTML PDF
Unit 8.7 Isolation of Proteins for Microsequence Analysis
Full Text: HTML PDF
Unit 9.5 Use of Bromoacetylation for Preparing Conformationally Constrained Cyclic and Polymeric Peptides and Peptide-Carrier Conjugates
Unit 10.20 Enzymatic Amplification of DNA by PCR
Unit 10.21 Quantitation of Rare DNAs by PCR
Unit 10.22 Detection of Isotype Switch Rearrangement in Bulk Culture by PCR
Abstract | Full Text: HTML PDF
Unit 10.23 Detection of Cytokine mRNA Expression by PCR
Abstract | Full Text: HTML PDF
Appendix 1 Abbreviations and Useful Data
Supplement 3, September 1992
Unit 3.7 Enrichment of Dendritic Cells
Unit 3.17 Morphological and Biochemical Assays of Apoptosis
Unit 10.12 Analysis of RNA by Northern and Slot-Blot Hybridization
Unit 10.13 Calcium Phosphate Transfection
Abstract | Full Text: HTML PDF
Unit 10.14 Transfection Using DEAE-Dextran
Abstract | Full Text: HTML PDF
Unit 10.15 Transfection by Electroporation
Abstract | Full Text: HTML PDF
Unit 10.16 Liposome-Mediated Transfection
Abstract | Full Text: HTML PDF
Unit 10.17 Use of Monoclonal Antibodies for Expression Cloning
Unit 10.18 Use of Monoclonal Antibodies for Expression Cloning
Abstract | Full Text: HTML PDF
Unit 11.1 Analysis of Inositol Phospholipid Turnover During Lymphocyte Activation
Full Text: HTML PDF
Unit 11.2 Preparation and Analysis of Phosphorylated Proteins
Abstract | Full Text: HTML PDF
Unit 11.3 Antiphosphotyrosine Blotting
Full Text: HTML PDF
Appendix 1 Abbreviations and Useful Data
Supplement 2, June 1992
Unit 1.10 Survival Surgery: Removal of the Spleen or Thymus
Abstract | Full Text: HTML PDF
Unit 4.4 Skin Allograft Rejection
Full Text: HTML PDF
Unit 6.13 Measurement of Mouse and Human Interleukin 9
Unit 6.14 Measurement of Mouse and Human Interleukin 10
Unit 6.15 Measurement of Human Interleukin 11
Unit 10.1 Purification and Concentration of DNA from Aqueous Solutions
Unit 10.2 Preparation of Genomic DNA from Mammalian Tissue
Abstract | Full Text: HTML PDF
Unit 10.3 Preparation of Bacterial Plasmid DNA
Unit 10.4 Agarose Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 10.5 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
Unit 10.6 Southern Hybridization Using Nylon Filters
Unit 10.7 Deprotection of Oligonucleotides and Purification Using Denaturing PAGE
Abstract | Full Text: HTML PDF
Unit 10.8 Digestion of DNA with Restriction Endonucleases
Abstract | Full Text: HTML PDF
Unit 10.9 Reagents and Radioisotopes Used to Manipulate Nucleic Acids
Abstract | Full Text: HTML PDF
Unit 10.10 Enzymatic Labeling of Nucleic Acids
Abstract | Full Text: HTML PDF
Appendix 3K Silinazing GlasswareAutoradiography
Supplement 1, March 1992
Unit 2.2 Isotype Determination of Antibodies
Abstract | Full Text: HTML PDF
Unit 2.9 Purification of Immunoglobulin M
Unit 2.10 Fragmentation of Immunoglobulin M
Unit 3.16 Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation
Unit 6.12 Measurement of Human Neutrophil Attractant Protein-1 (NAP-1; IL8)
Unit 7.23 Isolation and Functional Analysis of Neutrophils
Unit 7.24 Isolation of Human Basophils
Unit 7.25 Isolation of Tissue Mast Cells
Abstract | Full Text: HTML PDF
Unit 9.1 Peptide Synthesis
Unit 9.2 Purification and Analysis of Peptides
Unit 9.3 Selection of Immunogenic Peptides for Antisera Production
Unit 9.4 Production of Antipeptide Antisera
Core Publication, 1991
Unit 1.1 Animal Health Assurance
Unit 1.2 Managing Immunocompromised Animals
Unit 1.3 Handling and Restraint
Unit 1.4 Anesthesia
Unit 1.5 Animal Identification
Unit 1.6 Parenteral Injections
Unit 1.7 Blood Collection
Unit 1.8 Euthenasia
Unit 1.9 Removal of Lymphoid Organs
Abstract | Full Text: HTML PDF
Unit 2.1 Enzyme-Linked Immunosorbent Assays
Abstract | Full Text: HTML PDF
Unit 2.2 Isotype Determination of Antibodies
Unit 2.3 Double-Immunodiffusion Assay for Detecting Specific Antibodies
Abstract | Full Text: HTML PDF
Unit 2.4 Production of Polyclonal Antisera in Rabbits, Rats, Mice, and Hamsters
Unit 2.5 Production of Monoclonal Antibodies
Full Text: HTML PDF
Unit 2.6 Monoclonal Antibody Supernatant and Ascites Fluid Production
Unit 2.7 Purification of Immunoglobin G
Unit 2.8 Fragmentation of Immunoglobin G
Unit 3.1 Isolation of Mouse Mononuclear Cells
Abstract | Full Text: HTML PDF
Unit 3.2 T Cell Enrichment By Nonadherence to Nylon
Abstract | Full Text: HTML PDF
Unit 3.3 T Cell Enrichment by Cytotoxic Elimination of B Cells and Accessory Cells
Abstract | Full Text: HTML PDF
Unit 3.4 T Cell Depletion by Cytotoxic Elimination
Abstract | Full Text: HTML PDF
Unit 3.5 Fractionation of T Cells and B Cells Using Panning Techniques
Unit 3.6 Depletion of Accessory Cells by Adherence to Sephadex G-10
Abstract | Full Text: HTML PDF
Unit 3.7 Enrichment of Accessory Cells by Glass Adherence and Radioresistance
Unit 3.8 In Vitro Antibody Production
Unit 3.9 Early Parameters of B Cell Activation
Unit 3.10 Proliferative Assays for B Cell Function
Abstract | Full Text: HTML PDF
Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
Unit 3.12 Proliferative Assay for T Cell Function
Unit 3.13 Production of T Cell Clones
Unit 3.14 Production of Mouse T Cell Hydridomas
Unit 3.15 Quantitation of Functional T Cells by Limiting Dilution
Abstract | Full Text: HTML PDF
Unit 4.1 In Vivo Depletion of CD4- and CD8-Specific T Cells
Abstract | Full Text: HTML PDF
Unit 4.2 Contact Hypersensitivity
Abstract | Full Text: HTML PDF
Unit 4.3 Graft-versus-Host Disease
Unit 5.1 Overview of Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 5.2 Analysis of Flow Cytometry Data
Abstract | Full Text: HTML PDF
Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan
Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
Unit 5.6 Measurement of Intercellular Conjugates by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 5.7 Use of Flow Cytometry for DNA Analysis
Unit 5.8 Identification of Cells in Tissue Sections
Unit 6.1 Measurement of Lymphokine Receptors
Unit 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1
Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4
Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Growth Factors
Unit 6.5 Measurement of Mouse and Human Interleukin 5
Abstract | Full Text: HTML PDF
Unit 6.6 Measurement of Mouse and Human Interleukin 6
Unit 6.7 Measurement of Interleukin 7
Unit 6.8 Measurement of Mouse and Human Interferon γ
Abstract | Full Text: HTML PDF
Unit 6.9 Measurement of Antiviral Activity Induced by Interferons α, β, and γ
Abstract | Full Text: HTML PDF
Unit 6.10 Measurement of Tumor Necrosis Factor α and β
Abstract | Full Text: HTML PDF
Unit 6.11 Measurement of Transforming Growth Factor β
Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood
Unit 7.2 Isolation of T Cells Using Rosetting Procedures
Abstract | Full Text: HTML PDF
Unit 7.3 Purification of T Cell Subpopulations
Abstract | Full Text: HTML PDF
Unit 7.4 Immunomagnetic Purification of T Cell Subpopulations
Text: HTML PDF
Unit 7.5 Isolation of B Cell Population
Unit 7.6 Isolation of Monocyte/Macrophage Populations
Full Text: HTML PDF
Unit 7.7 Isolation of Cell Populations Containing NK Cells and LAK Precursor Cells
Unit 7.8 Isolation of Mononuclear Cells From Tonsillar Tissue
Full Text: HTML PDF
Unit 7.9 Detection of Unseparated Human Lymphocytes by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
Full Text: HTML PDF
Unit 7.11 Induction of Polyclonal Immunoglobulin Synthesis
Abstract | Full Text: HTML PDF
Unit 7.12 Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
Abstract | Full Text: HTML PDF
Unit 7.13 Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
Abstract | Full Text: HTML PDF
Unit 7.14 Measurement of Polyclonal Immunoglobin Synthesis Using the ELISPOT Assay
Unit 7.15 Measurement of In Vitro Specific-Antibody Synthesis
Abstract | Full Text: HTML PDF
Unit 7.16 Induction and Measurement of In Vivo Antibody Responses
Abstract | Full Text: HTML PDF
Unit 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
Unit 7.18 Measurement of Cytotoxic NK/LAK Cells
Unit 7.19 Generation and Maintenance of T Cell Lines and Clones
Unit 7.20 Generation of HTLV-I-Transformed T Cell Lines
Unit 7.21 Generation of T Cell Hybridomas
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Unit 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
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Unit 8.1 Solubilization of Lymphocytes
Unit 8.2 Immunoaffinity Chromatography
Unit 8.3 Immunoprecipitation
Unit 8.4 One-Dimensional Gel Electrophoresis of Proteins
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Unit 8.5 Two-Dimensional Gel Electrophoresis Using the O'Farrell System
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Unit 8.6 Diagonal Gel Electrophoresis
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Unit 8.7 Isolation of Proteins for Microsequence Analysis
Unit 8.8 Electroelution of Proteins from Stained Gels
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Unit 8.9 Staining Proteins in Gels
Unit 8.10 Immunoblotting
Unit 8.11 Iodination of Soluble and Membrane-Bound Proteins
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Unit 8.12 Biosynthetic Labeling of Proteins
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Appendix 1 Abbreviations and Useful Data
Appendix 2 Commonly Used Media, Buffers, and Stock Solutions
Appendix 3A Monitoring Cell Growth
Appendix 3B Trypan Blue Exclusion Test of Cell Viability
Appendix 3C Wright-Giemsa and Nonspecific Esterase Staining of Cells
Appendix 3D Labeling Cells in Microtiter Plates for Determination of [ 3 H]Thymidine Uptake
Appendix 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
Appendix 3F Obtaining Human Peripheral Blood Cells
Appendix 3G Cryopreservation of Cells
Appendix 3H Dialysis and Concentration of Protein Solutions
Appendix 3I Column Chromatography of Antibodies and Fragments
Appendix 3J Autoradiography
Appendix 4 The CD System of Leukocyte Surface Molecules