Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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Current Protocols in Immunology Supplements

2014:    Supplement 107     Supplement 106     Supplement 105     Supplement 104    
2013:    Supplement 103     Supplement 102     Supplement 101     Supplement 100    
2012:    Supplement 99     Supplement 98     Supplement 97     Supplement 96    
2011:    Supplement 95     Supplement 94     Supplement 93     Supplement 92    
2010:    Supplement 91     Supplement 90     Supplement 89     Supplement 88    
2009:    Supplement 87     Supplement 86     Supplement 85     Supplement 84    
2008:    Supplement 83     Supplement 82     Supplement 81     Supplement 80
2007:    Supplement 79     Supplement 78     Supplement 77     Supplement 76    
2006:    Supplement 75     Supplement 74     Supplement 73     Supplement 72     Supplement 71    
2005:    Supplement 70     Supplement 69     Supplement 68     Supplement 67     Supplement 66     Supplement 65    
2004:    Supplement 64     Supplement 63     Supplement 62     Supplement 61     Supplement 60     Supplement 59    
2003:    Supplement 58     Supplement 57     Supplement 56     Supplement 55     Supplement 54     Supplement 53    
2002:    Supplement 52     Supplement 51     Supplement 50     Supplement 49     Supplement 48     Supplement 47    
2001:    Supplement 46     Supplement 45     Supplement 44     Supplement 43     Supplement 42     Supplement 41    
2000:    Supplement 40     Supplement 39     Supplement 38     Supplement 37     Supplement 36     Supplement 35    
1999:    Supplement 34     Supplement 33     Supplement 32     Supplement 31     Supplement 30     Supplement 29    
1998:    Supplement 28     Supplement 27     Supplement 26     Supplement 25    
1997:    Supplement 24     Supplement 23     Supplement 22     Supplement 21    
1996:    Supplement 20     Supplement 19     Supplement 18     Supplement 17    
1995:    Supplement 16     Supplement 15     Supplement 14     Supplement 13    
1994:    Supplement 12     Supplement 11     Supplement 10     Supplement 9    
1993:    Supplement 8       Supplement 7       Supplement 6       Supplement 5    
1992:    Supplement 4       Supplement 3       Supplement 2       Supplement 1    
1991:    Core publication




  Supplement 107, November 2014

Unit 3.26 Isolation of Thymic Epithelial Cells and Analysis by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.41 Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis
         Abstract | Full Text:  HTML   PDF

Unit 15.27 T Cell Mediated Autoimmune Glomerular Disease in Mice
         Abstract | Full Text:  HTML   PDF

Unit 16.4 Isolation and Purification of Proteasomes from Primary Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 106, August 2014

Unit 2.18 Oil-Based Emulsion Vaccine Adjuvants
         Abstract | Full Text:  HTML   PDF

Unit 3.25 Isolation and Characterization of Mouse Innate Lymphoid Cells
         Abstract | Full Text:  HTML   PDF

Unit 5.10 Measurement of Peptide Binding to MHC Class II Molecules by Fluorescence Polarization
         Abstract | Full Text:  HTML   PDF

Unit 15.26 Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen
         Abstract | Full Text:  HTML   PDF

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  Supplement 105, April 2014

Unit 3.22 Isolation of Murine Natural Killer Cells
         Abstract | Full Text:  HTML   PDF

Unit 10.34 Immunologic Applications of Conditional Gene Modification Technology in the Mouse
         Abstract | Full Text:  HTML   PDF

Unit 14.36 Immunomagnetic Isolation of Pathogen-Containing Phagosomes and Apoptotic Blebs from Primary Phagocytes
         Abstract | Full Text:  HTML   PDF

Unit 19.6 Animal Models for Candidiasis
         Abstract | Full Text:  HTML   PDF

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  Supplement 104, February 2014

Unit 11.11 Experimental and Computational Tools for Analysis of Signaling Networks in Primary Cells
         Abstract | Full Text:  HTML   PDF

Unit 14.35 Isolation and Analysis of Mouse Microglial Cells
         Abstract | Full Text:  HTML   PDF

Unit 15.25 Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
         Abstract | Full Text:  HTML   PDF

Unit 22F.5 Differentiation and Characterization of Myeloid Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 103, November 2013

Unit 2.17 Single-Domain Antibodies and Their Utility
         Abstract | Full Text:  HTML   PDF

Unit 19.1 Schistosomiasis
         Abstract | Full Text:  HTML   PDF

Unit 21.4 Immunohistochemistry
         Abstract | Full Text:  HTML   PDF

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  Supplement 102, October 2013

Unit 2.5 Production of Monoclonal Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 12.14 Nonhuman Primate Models for HIV/AIDS Vaccine Development
         Abstract | Full Text:  HTML   PDF

Unit 14.34 Dynamic Quantitative Assays of Phagosomal Function
         Abstract | Full Text:  HTML   PDF

Unit 22F.12 A Practical Guide to Culturing Mouse and Human Bone Marrow Stromal Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 101, April 2013

Unit 14.29 Target-Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
         Abstract | Full Text:  HTML   PDF

Unit 14.33 Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 15.14 Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy
         Abstract | Full Text:  HTML   PDF

Unit 15.23 Mouse Model for Pyelonephritis
         Abstract | Full Text:  HTML   PDF

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  Supplement 100, February 2013

Unit 12.13 Evaluation of B Cell Function in Patients with HIV
         Abstract | Full Text:  HTML   PDF

Unit 14.31 Measurement of Phagocytic Engulfment of Apoptotic Cells by Macrophages Using pHrodo Succinimidyl Ester
         Abstract | Full Text:  HTML   PDF

Unit 14.32 Comparing Individual NK Cell Activity In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 15.8 Experimental Autoimmune Myasthenia Gravis in the Mouse
         Abstract | Full Text:  HTML   PDF

Unit 18.3 Measurement of MHC/Peptide Interactions by Gel Filtration or Monoclonal Antibody Capture
         Abstract | Full Text:  HTML   PDF

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  Supplement 99, November 2012

Unit 3.19 Isolation of Mouse Lymphocytes from Small Intestine Tissues
         Abstract | Full Text:  HTML   PDF

Unit 7.32 Isolation and Generation of Human Dendritic Cells
         Abstract | Full Text:  HTML   PDF

Unit 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
         Abstract | Full Text:  HTML   PDF

Unit 14.30 Measurement of Phagocytosis, Phagosome Acidification, and Intracellular Killing of Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Appendix 3G Cryopreservation and Thawing of Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 98, August 2012

Unit 3.24 Generation and Characterization of Mouse Basophils from Bone Marrow and Purification of Basophils from Spleen
         Abstract | Full Text:  HTML   PDF

Unit 7.31 Isolation of Human Eosinophils
         Abstract | Full Text:  HTML   PDF

Unit 22F.11 Differentiation and Characterization of Dendritic Cells from Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 97, April 2012

Unit 7.40 Isolation of Cells from the Feto-Maternal Interface
         Abstract | Full Text:  HTML   PDF

Unit 14.29 Target-Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
         Abstract | Full Text:  HTML   PDF

Unit 18.18 Analysis of the Targeting Properties of Fc Chimeric Proteins and Antibodies in Mice In Vivo
         Abstract | Full Text:  HTML   PDF

Unit 22F.10 Differentiation of Mast Cells from Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 96, February 2012

Unit 8.17 Single-Cell Phospho-Protein Analysis by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 14.28 Analysis of Gene Expression and Gene Silencing in Human Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 15.24 Mouse Models of Atherosclerosis
         Abstract | Full Text:  HTML   PDF

Unit 19.3 Animal Models for Toxoplasma gondii Infection
         Abstract | Full Text:  HTML   PDF

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  Supplement 95, November 2011

Unit 7.21B Immortalization of Human and Rhesus Macaque Primary Antigen-Specific T Cells by Retrovirally Transduced Telomerase Reverse Transcriptase
         Abstract | Full Text:  HTML   PDF

Unit 14.26 Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated by Phagocytes
         Abstract | Full Text:  HTML   PDF

Unit 14.27 Measuring Opsonic Phagocytosis via Fcγ Receptors and Complement Receptors on Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 15.23 Experimental Autoimmune Myasthenia Gravis in the Mouse
         Abstract | Full Text:  HTML   PDF

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  Supplement 94, August 2011

Unit 7.5 Isolation of Human B Cell Populations
         Abstract | Full Text:  HTML   PDF

Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 10.33 Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR
         Abstract | Full Text:  HTML   PDF

Unit 19.5 Animal Models of Mycobacteria Infection
         Abstract | Full Text:  HTML   PDF

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  Supplement 93, April 2011

Unit 11.7 Protein Tyrosine Phosphatase Assays
         Abstract | Full Text:  HTML   PDF

Unit 14.22 Quantitative Assessment of Macrophage Functions in Repair and Fibrosis
         Abstract | Full Text:  HTML   PDF

Unit 14.23 Measuring the Killing of Intracellular Pathogens: Leishmania
         Abstract | Full Text:  HTML   PDF

Unit 14.24 Measuring Matrix Metalloproteinase Activity in Macrophages and Polymorphonuclear Leukocytes
         Abstract | Full Text:  HTML   PDF

Unit 14.25 Measurement of Macrophage-Mediated Killing of Intracellular Bacteria, Including Francisella and Mycobacteria
         Abstract | Full Text:  HTML   PDF

Unit 19.4 Experimental Asexual Blood Stage Malaria Immunity
         Abstract | Full Text:  HTML   PDF

Unit 19.14 Infection of Mice with Francisella as an Immunological Model
         Abstract | Full Text:  HTML   PDF

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  Supplement 92, February 2011

Unit 14.18 Measurement of Tumor Cytolysis by Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.19 In Vivo and Ex Vivo Protocols for Measuring the Killing of Extracellular Pathogens by Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.20 Measurement of Macrophage Growth and Differentiation
         Abstract | Full Text:  HTML   PDF

Unit 14.21 Measurement of Type I Interferon Production
         Abstract | Full Text:  HTML   PDF

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  Supplement 91, November 2010

Unit 7.38 Measuring Mast Cell Mediator Release
         Abstract | Full Text:  HTML   PDF

Unit 7.39 Evaluation of Human Natural Killer Cell Activities in Whole Blood
         Abstract | Full Text:  HTML   PDF

Unit 10.17E Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models
         Abstract | Full Text:  HTML   PDF

Unit 14.16 Measuring TLR Function in Transfectants
         Abstract | Full Text:  HTML   PDF

Unit 14.17 Measurement of Myeloid Cell Immune Suppressive Activity
         Abstract | Full Text:  HTML   PDF

Unit 19.13 Cecal Ligation and Puncture
         Abstract | Full Text:  HTML   PDF

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  Supplement 90, August 2010

Unit 7.25 Isolation of Tissue Mast Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.37 Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines Derived from Peripheral Blood or Cord Blood
         Abstract | Full Text:  HTML   PDF

Unit 9.9 Epitope Mapping Using Gram-Positive Surface Display
         Abstract | Full Text:  HTML   PDF

Unit 11.9B Signal Transduction During Activation and Inhibition of Natural Killer Cells
         Abstract | Full Text:  HTML   PDF

Unit 14.11 Isolation and Functional Use of Human NKT Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 89, April 2010

Unit 2.16 Efficient Delivery of Antibody Into Living Cells Using Hemagglutinating Virus of Japan (HVJ) Envelope
         Abstract | Full Text:  HTML   PDF

Unit 8.15 Endoglycosidase and Glycoamidase Release of N-Linked Glycan
         Abstract | Full Text:  HTML   PDF

Unit 10.17D Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Detecting Tyrosine-Phosphorylated Proteins by Western Blot Analysis
         Abstract | Full Text:  HTML   PDF

Unit 15.5 Collagen-Induced Arthritis
         Abstract | Full Text:  HTML   PDF

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  Supplement 88, February 2010

Unit 14.15 Monocyte and Neutrophil Isolation and Migration Assays
         Abstract | Full Text:  HTML   PDF

Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
         Abstract | Full Text:  HTML   PDF

Unit 16.1 Choosing and Preparing Antigen-Presenting Cells
         Abstract | Full Text:  HTML   PDF

Unit 16.2 Presenting Exogenous Antigens to T Cells
         Abstract | Full Text:  HTML   PDF

Unit 18.17 The MHC Motif Viewer: A Visualization Tool for MHC Binding Motifs
         Abstract | Full Text:  HTML   PDF

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  Supplement 87, November 2009

Unit 5.9 Visualization of Multiprotein Complexes by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Phosphoinositide and Inositol Phosphate Analysis in Lymphocyte Activation
         Abstract | Full Text:  HTML   PDF

Unit 11.9D TRAF-Mediated TNFR-Family Signaling
         Abstract | Full Text:  HTML   PDF

Unit 14.14 Measuring Autophagy in Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 17.5 Preparation of Stable Single-Chain Trimers Engineered with Peptide, β2 Microglobulin, and MHC Heavy Chain
         Abstract | Full Text:  HTML   PDF

Unit 18.16 Generation of Peptide MHC Class I Monomers and Multimers Through Ligand Exchange
         Abstract | Full Text:  HTML   PDF

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  Supplement 86, August 2009

Unit 3.7 Isolation of Dendritic Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.8 Isolation of Mononuclear Cells from Tonsillar Tissue
         Abstract | Full Text:  HTML   PDF

Unit 9.8 Phage Display Selection, Analysis, and Prediction of B Cell Epitopes
         Abstract | Full Text:  HTML   PDF

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  Supplement 85, April 2009

Unit 2.9 Isolation of Murine and Human Immunoglobin M and Murine Immunoglobin D
         Abstract | Full Text:  HTML   PDF

Unit 2.9B Measurement of Human Serum IgD Levels
         Abstract | Full Text:  HTML   PDF

Unit 2.15 Vaccination of Mice with Baculovirus-Infected Insect Cells Expressing Antigenic Proteins
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
         Abstract | Full Text:  HTML   PDF

Unit 7.4 Immunomagnetic Purification of T Cell Subpopulations
         Abstract | Full Text:  HTML   PDF

Unit 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
         Abstract | Full Text:  HTML   PDF

Unit 20.11 The Immune Response to Tumors
         Abstract | Full Text:  HTML   PDF

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  Supplement 84, February 2009

Unit 4.4 Skin Allograft Rejection
         Abstract | Full Text:  HTML   PDF

Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
         Abstract | Full Text:  HTML   PDF

Unit 14.10 Human Natural Killers Receptors, Co-Receptors, and Their Ligands
         Abstract | Full Text:  HTML   PDF

Unit 20.10 Molecular Cloning and Characterization of MHC Class I- and II-Restricted Tumor Antigens Recognized by T Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 83, November 2008

Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
         Abstract | Full Text:  HTML   PDF

Unit 8.10 Immunoblotting and Immunodetection
         Abstract | Full Text:  HTML   PDF

Unit 8.10B Detection of Proteins on Blot Transfer Membranes
         Abstract | Full Text:  HTML   PDF

Unit 8.15 Endoglycosidase and Glycoamidase Release of N-Linked Oligosaccharides
         Full Text:  HTML   PDF

Unit 14.1 The Isolation and Characterization of Murine Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.2 Activation of Murine Macrophages
         Abstract | Full Text:  HTML   PDF

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  Supplement 82, August 2008

Unit 1.12 Techniques in Aseptic Rodent Surgery
         Abstract | Full Text:  HTML   PDF

Unit 2.4 Production of Polyclonal Antisera
         Abstract | Full Text:  HTML   PDF

Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 20.9 ErbB2 Transgenic Mice: A Tool for Investigation of the Immune Prevention and Treatment of Mammary Carcinomas
         Abstract | Full Text:  HTML   PDF

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  Supplement 81, May 2008

Unit 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras
         Abstract | Full Text:  HTML   PDF

Unit 15.21 Creation of "Humanized" Mice to Study Human Immunity
         Abstract | Full Text:  HTML   PDF

Unit 15.22 The K/BxN Arthritis Model
         Abstract | Full Text:  HTML   PDF

Unit 18.15 Characterizing Protein-Protein Interactions by Sedimentation Velocity Analytical Ultracentifrugation
         Abstract | Full Text:  HTML   PDF

Appendix 1X Paired Receptor Systems of the Innate Immune System
         Abstract | Full Text:  HTML   PDF

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  Supplement 80, February 2008

Unit 20.8 Carcinoembryonic Antigen Transgenic Mouse Models for Immunotherapy and Development of Cancer Vaccines
         Abstract | Full Text:  HTML   PDF

Unit 22B.2 Characterization of Mouse Hematopoietic Stem and Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
         Abstract | Full Text:  HTML   PDF

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  Supplement 79, November 2007

Unit 6.25 Measurement of Interleukin-17
         Abstract | Full Text:  HTML   PDF

Unit 15.18 Animal Models of Airway Sensitization
         Abstract | Full Text:  HTML   PDF

Unit 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
         Abstract | Full Text:  HTML   PDF

Unit 22A.2 Sources of Human and Murine Hematopoietic Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 78, August 2007

Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.30 Measurement of Interleukin-21
         Abstract | Full Text:  HTML   PDF

Unit 8.17 Single-Cell Phospho-Protein Analysis by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 15.7 Experimental Autoimmune Thyroiditis in the Mouse
         Abstract | Full Text:  HTML   PDF

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  Supplement 77, May 2007

Unit 1.2 Managing Immunocompromised Animals
         Abstract | Full Text:  HTML   PDF

Unit 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
         Abstract | Full Text:  HTML   PDF

Unit 10.32 Use of Bacterial Artificial Chromosomes in Generating Targeted Mutations in Human and Mouse Cytomegaloviruses
         Abstract | Full Text:  HTML   PDF

Unit 14.12 Toll-Like Receptors
         Abstract | Full Text:  HTML   PDF

Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
         Full Text:  HTML   PDF

Unit 16.3 Targeted Identification of Infection-Related HLA Class I–Presented Epitopes by Stable Isotope Tagging of Epitopes (SITE)
         Abstract | Full Text:  HTML   PDF

Unit 18.14 Measurement of Peptide Dissociation from MHC Class II Molecules
         Abstract | Full Text:  HTML   PDF

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  Supplement 76, February 2007

Unit 1.1 Animal Health Assurance
         Abstract | Full Text:  HTML   PDF

Unit 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Detection of Protein-Protein Interactions by Coprecipitation
         Abstract | Full Text:  HTML   PDF

Unit 18.13 Supported Planar Bilayers for Study of the Immunological Synapse
         Abstract | Full Text:  HTML   PDF

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  Supplement 75, November 2006

Unit 7.5 Isolation of Human B Cell Populations
         Abstract | Full Text:  HTML   PDF

Unit 8.4 One-Dimensional SDS Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF

Unit 22F.9 Differentiation of Multipotent Adult Progenitor Cells into Functional Endothelial and Smooth Muscle Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 74, August 2006

Unit 2.5 Production of Monoclonal Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 3.23 Generation, Isolation, and Maintenance of Rodent Mast Cells and Mast Cell Lines
         Abstract | Full Text:  HTML   PDF

Appendix 1Q Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

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  Supplement 73, June 2006

Unit 1.3 Handling and Restraint
         Abstract | Full Text:  HTML   PDF

Unit 1.5 Animal Identification
         Abstract | Full Text:  HTML   PDF

Unit 1.6 Parenteral Injections
         Abstract | Full Text:    PDF

Unit 1.7 Blood Collection
         Abstract | Full Text:  HTML   PDF

Unit 1.8 Euthanasia
         Abstract | Full Text:  HTML   PDF

Unit 14.13 Characterization and Functional Analysis of Mouse Invariant Natural T (iNKT) Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 72, April 2006

Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
         Abstract | Full Text:  HTML   PDF

Unit 3.13 Production of T H 1 and T H 2 Cell Lines and Clones
         Abstract | Full Text:  HTML   PDF

Unit 7.4B The Purification and Functional Analysis of Human CD4 CD25 high Regulatory T Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 71, February 2006

Unit 3.18 In Vitro Systems for the Study of T Cell Development: Fetal Thymus Organ Culture and OP9-DL1 Cell Coculture
         Abstract | Full Text:  HTML   PDF

Appendix 1J Web Sites of Interest to Immunologists
         Abstract | Full Text:  HTML   PDF

Appendix 1W Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors
         Abstract | Full Text:  HTML   PDF

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  Supplement 70, December 2005

Unit 7.6A Isolation of Human Monocyte Populations
         Abstract | Full Text:  HTML   PDF

Unit 7.6B Isolation and Purification of Human Intestinal Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Culture of HIV in Monocytes and Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 12.5 Detection Assays for HIV Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 69, October 2005

Unit 12.3 Infection of CD4 Primary T Cells and Cell Lines, Generation of Chronically Infected Cell Lines, and Induction of HIV Expression
         Abstract | Full Text:  HTML   PDF

Unit 22A.1 Large Animal Models for Stem and Progenitor Cell Analysis
         Abstract | Full Text:  HTML   PDF

Unit 22F.7 Differentiation of Human Erythroid Cells in Culture
         Abstract | Full Text:  HTML   PDF

Unit 22F.8 Differentiation and Characterization of T Cells
         Abstract | Full Text:  HTML   PDF

Appendix 1V Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
         Abstract | Full Text:  HTML   PDF

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  Supplement 68, August 2005

Unit 8.5 Two-Dimensional Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 13.8 Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
         Abstract | Full Text:  HTML   PDF

Unit 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
         Abstract | Full Text:  HTML   PDF

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  Supplement 67, June 2005

Unit 13.7 Assays for Complement Proteins Encoded in the Class III Region of Human MHC
         Abstract | Full Text:  HTML   PDF

Unit 22B.1 Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 22F.4 Differentiation of Peripheral Blood Monocytes into Dendritic Cells
         Abstract | Full Text:  HTML   PDF

Unit 22F.5 Differentiation and Characterization of Myeloid Cells
         Abstract | Full Text:  HTML   PDF

Unit 22F.6 Culture, Expansion, and Differentiation of Murine Megakaryocytes
         Abstract | Full Text:  HTML   PDF

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  Supplement 66, April 2005

Unit 7.18B Measurement of Cytokine Production Using Whole Blood
         Abstract | Full Text:  HTML   PDF

Unit 22A.1 Sources and Methods for Obtaining Stem and Progenitor Cells
         Full Text:  HTML   PDF

Unit 22F.1 Isolation of Prolymphocytes from Bone Marrow and Fetal Liver
         Abstract | Full Text:  HTML   PDF

Unit 22F.2 In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture
         Abstract | Full Text:  HTML   PDF

Unit 22F.3 Measurement of Natural Killer Cell Progenitor Activity in Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 65, February 2005

Unit 7.36 In Vitro Models of Human T Cell Anergy
         Abstract | Full Text:  HTML   PDF

Unit 12.12 Immunophenotyping of T Cell Subpopulations in HIV Disease
         Abstract | Full Text:  HTML   PDF

Unit 20.7 Identification of Human Tumor Antigens by Serological Analysis of Recombinant cDNA Expression Libraries (SEREX)
         Abstract | Full Text:  HTML   PDF

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  Supplement 64, December 2004

Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.11 Evaluating Neutralizing Antibodies Against HIV, SIV, and SHIV in Luciferase Reporter Gene Assays
         Abstract | Full Text:  HTML   PDF

Unit 17.4 In Situ Staining Using MHC Class I Tetramers
         Abstract | Full Text:  HTML   PDF

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  Supplement 63, October 2004

Unit 7.21 Herpesvirus saimiri Transformation of Human T Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 7.35 Assays for B Cell and Germinal Center Development
         Abstract | Full Text:  HTML   PDF

Unit 10.31 Measurement of Mouse T Cell Receptor Excision Circles
         Abstract | Full Text:  HTML   PDF

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  Supplement 62, August 2004

Unit 10.30 Analysis of Telomere Length and Telomerase Activity
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Culture of HIV in Monocytes and Macrophages
         Full Text:  HTML   PDF

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  Supplement 61, June 2004

Unit 6.29 Cytokine, Growth Factor, and Chemokine Ligand Database
         Abstract | Full Text:  HTML   PDF

Unit 18.12 Competition-Based Cellular Peptide Binding Assay for HLA Class I
         Abstract | Full Text:  HTML   PDF

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  Supplement 60, April 2004

Unit 3.12 Proliferative Assays for T Cell Function
         Abstract | Full Text:  HTML   PDF

Unit 5.7 Analysis of Cellular DNA Content by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.34 Isolation and Characterization of Human Natural Killer Cell Subsets
         Abstract | Full Text:  HTML   PDF

Unit 9.4 B Cell Epitope Mapping Using Synthetic Peptides
         Abstract | Full Text:  HTML   PDF

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  Supplement 59, February 2004

Unit 3.17 Measurement of Apoptosis and Other Forms of Cell Death
         Abstract | Full Text:  HTML   PDF

Unit 12.1 General Guidelines for Experimenting with HIV
         Abstract | Full Text:  HTML   PDF

Appendix 1U Large-Scale Cell Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 58, December 2003

Unit 8.9 Staining Proteins in Gels
         Abstract | Full Text:  HTML   PDF

Unit 11.8 Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells
         Abstract | Full Text:  HTML   PDF

Unit 13.6 Assays for the Mannan-Binding Lectin Pathway
         Abstract | Full Text:  HTML   PDF

Appendix 1T Heat-Shock Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 57, October 2003

Unit 3.9 Early Events in B Lymphocyte Activation
         Abstract | Full Text:  HTML   PDF

Unit 8.1A Solubilization of Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 8.1C Subcellular Fractionation of Tissue Culture Cells
         Abstract | Full Text:  HTML   PDF

Unit 18.11 Peptide Induction of Surface Expression of Class I MHC
         Abstract | Full Text:  HTML   PDF

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  Supplement 56, August 2003

Unit 3.8 Assays for B Lymphocyte Function
         Abstract | Full Text:  HTML   PDF

Unit 8.1B Purification of Organelles from Mammalian Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 55, June 2003

Unit 3.5A Fractionation of T and B Cells Using Magnetic Beads
         Abstract | Full Text:  HTML   PDF

Unit 9.2 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins
         Abstract | Full Text:  HTML   PDF

Unit 9.3 Production of Anti-Peptide Antisera
         Abstract | Full Text:  HTML   PDF

Unit 19.12 Animal Model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus
         Abstract | Full Text:  HTML   PDF

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  Supplement 54, April 2003

Unit 6.28 The In Vivo Cytokine Capture Assay for Measurement of Cytokine Production in the Mouse
         Abstract | Full Text:  HTML   PDF

Unit 10.29 Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression
         Abstract | Full Text:  HTML   PDF

Unit 12.7 Regulation of the HIV Promoter/Enhancer
         Abstract | Full Text:  HTML   PDF

Unit 12.10 Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors
         Abstract | Full Text:  HTML   PDF

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  Supplement 53, February 2003

Unit 15.6 Experimental Autoimmune Uveoretinitis in the Rat and Mouse
         Abstract | Full Text:  HTML   PDF

Unit 17.3 MHC-Peptide Tetramers to Visualize Antigen-Specific T Cells
         Abstract | Full Text:  HTML   PDF

Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens
         Full Text:  HTML   PDF

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  Supplement 52, December 2002

Unit 14.11 Isolation and Functional Use of Human NK T Cells
         Abstract | Full Text:  HTML   PDF

Unit 14.12 Toll Receptors
         Full Text:  HTML   PDF

Unit 15.20 Animal Models for SLE
         Abstract | Full Text:  HTML   PDF

Unit 18.10 Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation and Molecular Proximity
         Abstract | Full Text:  HTML   PDF

Appendix 1S Nomenclature and Serology of HLA Class I and Class II Alleles
         Abstract | Full Text:  HTML   PDF

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  Supplement 51, October 2002

Unit 6.13 Measurement of Mouse and Human Interleukin 9
         Abstract | Full Text:  HTML   PDF

Unit 11.9D TRAF-Mediated TNFR-Family Signaling
         Full Text:  HTML   PDF

Unit 11.10 Isolation and Use of Rafts
         Abstract | Full Text:  HTML   PDF

Appendix 1R Receptors Involved in the Regulation of Murine NK Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 50, August 2002

Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry
         Full Text:  HTML   PDF

Unit 9.8 Phage Display Selection and Analysis of Ab-Binding Epitopes
         Full Text:  HTML   PDF

Unit 18.9 Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance
         Abstract | Full Text:  HTML   PDF

Appendix 3J Detection and Quantitation of Radiolabeled Proteins and DNA in Gels and Blots
         Abstract | Full Text:  HTML   PDF

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  Supplement 49, June 2002

Unit 5.8 Multiparameter FACS Analysis
         Abstract | Full Text:  HTML   PDF

Unit 7.5 Isolation of B Cell Populations
         Full Text:  HTML

Unit 9.2 Synthetic Peptides for Production of Antibodies that Recognize Intact Proteins

Unit 21.4 Immunohistochemistry
         Abstract | Full Text:  HTML   PDF

Appendix 1M Listing, Location, Binding Motifs, and Expression of Nonclassical Class I and Related Genes and Molecules
         Abstract | Full Text:  HTML   PDF

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  Supplement 48, April 2002

Unit 10.19A Construction of Immunoglobulin Fusion Proteins
         Abstract | Full Text:  HTML   PDF

Unit 10.19B Phage Display of Single-Chain Antibody Constructs
         Abstract | Full Text:  HTML   PDF

Unit 21.1 Proper Alignment and Adjustment of the Light Microscope
         Abstract | Full Text:  HTML   PDF

Unit 21.2 Fluorescence Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 21.3 Immunofluorescence Staining
         Abstract | Full Text:  HTML   PDF

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  Supplement 47, February 2002

Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions
         Abstract | Full Text:  HTML   PDF

Unit 7.19 Generation and Maintenance of Cloned Human T Cell Lines
         Abstract | Full Text:  HTML   PDF

Unit 7.20 Generation of HTLV-I-Transformed T Cell Lines
         Abstract | Full Text:  HTML   PDF

Unit 9.1 Introduction to Peptide Synthesis
         Abstract | Full Text:  HTML   PDF

Appendix 4B Monoclonal Antibodies to Mouse Cell-Surface Antigens
         Abstract | Full Text:  HTML   PDF

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  Supplement 46, December 2001

Unit 6.18 Measurement of Interleukin-13
         Abstract | Full Text:  HTML   PDF

Unit 6.27 Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay
         Abstract | Full Text:  HTML   PDF

Unit 8.9 Staining Proteins in Gels

Unit 14.10 Human Natural Killer Receptors and Their Ligands
         Full Text:  HTML   PDF

Unit 20.6 Models for Lymphoma
         Abstract | Full Text:  HTML   PDF

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  Supplement 45, October 2001

Unit 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries
         Abstract | Full Text:  HTML   PDF

Unit 15.19 Induction of TNBS Colitis in Mice
         Abstract | Full Text:  HTML   PDF

Unit 20.5 The TRAMP Mouse as a Model for Prostate Cancer
         Abstract | Full Text:  HTML   PDF

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  Supplement 44, August 2001

Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACS Calibur
         Abstract | Full Text:  HTML   PDF

Unit 6.26 Measurement of Human and Mouse Interleukin 18
         Abstract | Full Text:  HTML   PDF

Unit 11.9C Apoptosis Signaling Pathways
         Abstract | Full Text:  HTML   PDF

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  Supplement 43, June 2001

Unit 1.11 Surgical Removal of Mouse Salivary Glands
         Abstract | Full Text:  HTML   PDF

Unit 20.4 The P815 Mastocytoma Tumor Model
         Abstract | Full Text:  HTML   PDF

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  Supplement 42, April 2001

Unit 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
         Full Text:  HTML   PDF

Unit 12.4 Culture of HIV in Monocytes and Macrophages

Unit 12.5 Detection Assays for HIV Proteins
         Full Text:  HTML   PDF

Unit 19.11 Influenza Virus
         Abstract | Full Text:  HTML   PDF

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  Supplement 41, February 2001

Unit 6.11 Measurement of TGF-β in Biological Fluids
         Abstract | Full Text:  HTML   PDF

Unit 6.25 Measurement of Interleukin 17
         Full Text:  HTML   PDF

Unit 8.3 Immunoprecipitation
         Abstract | Full Text:  HTML   PDF

Appendix 1Q Safe Use of Radioisotopes
         Full Text:  HTML   PDF

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  Supplement 40, December 2000

Unit 2.6 Monoclonal Antibody Supernatant and Ascites Fluid Production
         Abstract | Full Text:  HTML   PDF

Unit 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
         Full Text:  HTML   PDF

Appendix 1O Nomenclature of the Human T Cell Receptor Genes
         Abstract | Full Text:  HTML   PDF

Appendix 1P Nomenclature of the Human Immunoglobulin Genes
         Abstract | Full Text:  HTML   PDF

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  Supplement 39, October 2000

Unit 9.4 Production of Anipeptide Antisera

Unit 20.1 B16 as a Mouse Model for Human Melanoma
         Abstract | Full Text:  HTML   PDF

Unit 20.2 Mouse 4T1 Breast Tumor Model
         Abstract | Full Text:  HTML   PDF

Unit 20.3 Mouse SaI Sarcoma Tumor Model
         Abstract | Full Text:  HTML   PDF

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  Supplement 38, August 2000

Unit 2.10A Fragmentation of Immunoglobulin M
         Abstract | Full Text:  HTML   PDF

Unit 2.10B Purification of Human IgA
         Abstract | Full Text:  HTML   PDF

Unit 6.12 Biological Responses to Chemokine Superfamily Members
         Abstract | Full Text:  HTML   PDF

Unit 10.28 Spectratype/Immunoscope Analysis of the Expressed TCR Repertoire
         Abstract | Full Text:  HTML   PDF

Unit 12.6 Detection of HIV DNA and RNA Using PCR
         Abstract | Full Text:  HTML   PDF

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  Supplement 37, June 2000

Unit 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay
         Abstract | Full Text:  HTML   PDF

Unit 11.10 Isolation and Use of Rafts

Appendix 1N Overview of Protein Folds in the Immune System
         Abstract | Full Text:  HTML   PDF

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  Supplement 36, April 2000

Unit 18.7 Biotin Labeling and Quantitation of Cell-Surface Proteins
         Abstract | Full Text:  HTML   PDF

Unit 19.8 Helicobacter Animal Models
         Abstract | Full Text:  HTML   PDF

Unit 19.9 Animal Model for Infection with Listeria monocytogenes
         Abstract | Full Text:  HTML   PDF

Unit 19.10 Animal Models Using Lymphocytic Choriomeningitis Virus
         Abstract | Full Text:  HTML   PDF

Appendix 1I Peptide Binding Motifs for MHC Class I and II Molecules
         Abstract | Full Text:  HTML   PDF

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  Supplement 35, February 2000

Unit 11.9A Key Molecules Involved in Receptor-Mediated Lymphocyte Activation
         Abstract | Full Text:  HTML   PDF

Unit 11.9B Signal Transduction During Natural Killer Cell Activation
         Abstract | Full Text:  HTML   PDF

Unit 17.2 Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers
         Abstract | Full Text:  HTML   PDF

Appendix 1J Web Sites of Interest to Immunologists
         Full Text:  HTML   PDF

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  Supplement 34, December 1999

Unit 9.6 Synthesis and Application of Peptide Dendrimers As Protein Mimetics
         Abstract | Full Text:  HTML   PDF

Unit 15.18 Animal Models of Airway Sensitization
         Full Text:  HTML   PDF

Unit 17.1 Bacteriophage Library Construction and Selection of Recombinant Antibodies
         Abstract | Full Text:  HTML   PDF

Appendix 2A Common Media, Buffers, and Stock Solutions
         Abstract | Full Text:  HTML   PDF

Appendix 2B Equipment
         Abstract | Full Text:  HTML   PDF

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  Supplement 33, October 1999

Unit 7.33 Measurement of Prostaglandins and Other Eicosanoids
         Abstract | Full Text:  HTML   PDF

Unit 18.5 Measurement of the Kinetics of Biomolecular Interactions Using the IAsys Resonant Mirror Biosensor
         Abstract | Full Text:  HTML   PDF

Unit 18.6 Affinity-Based Biosensors for Biomolecular Interaction Analysis
         Abstract | Full Text:  HTML   PDF

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  Supplement 32, August 1999

Unit 15.15 Mercuric Chloride–Induced Autoimmunity
         Abstract | Full Text:  HTML   PDF

Unit 15.16 The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases
         Abstract | Full Text:  HTML   PDF

Unit 15.17 Autoimmune Ovarian Disease Induced by Immunization with Zona Pellucida (ZP3) Peptide
         Abstract | Full Text:  HTML   PDF

Unit 19.7 A Mouse Model for Cytomegalovirus Infection
         Abstract | Full Text:  HTML   PDF

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  Supplement 31, June 1999

Unit 3.22 Isolation of Murine Natural Killer Cells
         Abstract | Full Text:  HTML   PDF

Unit 10.17C Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T Cell–Based Systems
         Abstract | Full Text:  HTML   PDF

Unit 15.14 Autoimmune Myocarditis
         Abstract | Full Text:  HTML   PDF

Unit 18.4 Measurement of Peptide-MHC Interactions in Solution Using the Spin Column Filtration Assay
         Abstract | Full Text:  HTML   PDF

Appendix 4C Monoclonal Antibodies to Rat Leukocyte Surface Antigens, MHC Antigens, and Immunoglobulins
         Abstract | Full Text:  HTML   PDF

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  Supplement 30, April 1999

Unit 15.12 Induction of Autoimmune Disease by Depletion of Regulatory T Cells
         Abstract | Full Text:  HTML   PDF

Unit 15.13 Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Depletion of Regulatory T Cells
         Abstract | Full Text:  HTML   PDF

Unit 19.5 Animal Models of Mycobacteria Infection
         Abstract | Full Text:  HTML   PDF

Unit 19.6 Animal Models for Candidiasis
         Abstract | Full Text:  HTML   PDF

Appendix 1G HLA Type of EBV-Transformed Human B Cell Lines
         Abstract |Full Text:  HTML   PDF

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  Supplement 29, February 1999

Unit 15.11 Animal Models of IgA Nephropathy
         Abstract | Full Text:  HTML   PDF

Unit 19.4 Experimental Asexual Blood Stage Malaria Immunity
         Abstract | Full Text:  HTML   PDF

Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens

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  Supplement 28, December 1998

Unit 11.8 Measurement of Mitogen-Activated Protein Kinase (MAPK) in Activated T Cells

Unit 19.1 Schistosomiasis
         Abstract | Full Text:  HTML   PDF

Unit 19.2 Animal Models for the Analysis of Immune Responses to Leishmaniasis
         Abstract | Full Text:  HTML   PDF

Unit 19.3 Animal Models for Toxoplasma gondii Infection
         Abstract | Full Text:  HTML   PDF

Appendix 1M Apoptosis Signaling Pathways

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  Supplement 27, September 1998

Unit 1.4 Anesthesia
         Abstract | Full Text:  HTML   PDF

Unit 2.14 Nucleic Acid Immunizations
         Abstract | Full Text:  HTML   PDF

Unit 4.3 Animal Models of Acute and Chronic Graft-Versus-Host Disease
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Preparation of Bacterial Plasmid DNA
         Abstract | Full Text:  HTML   PDF

Appendix 1K Human MHC Genes and Products
         Abstract | Full Text:  HTML   PDF

Appendix 1L Mouse MHC Genes and Products
         Abstract | Full Text:  HTML   PDF

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  Supplement 26, June 1998

Unit 6.21 Detection of Cytokine Receptors by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry

Unit 8.10 Immunoblotting and Immunodetection
         Full Text:  HTML   PDF

Unit 15.10 Streptococcal Cell Wall Arthritis
         Abstract | Full Text:  HTML   PDF

Unit 18.2 Detection of Low-Affinity Ligand-Receptor Interactions at the Cell Surface with Fluorescent Microspheres
         Abstract | Full Text:  HTML   PDF

Unit 18.3 Measurement of MHC/Peptide Interactions by Gel Filtration
         Abstract | Full Text:  HTML   PDF

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  Supplement 25, March, 1998

Unit 3.7 Isolation of Dendritic Cells
         Full Text:  HTML   PDF

Unit 3.16 Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation
         Abstract | Full Text:  HTML   PDF

Unit 3.17 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis

Unit 4.8 Construction of Human-SCID Chimeric Mice
         Abstract | Full Text:  HTML   PDF

Unit 18.1 Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces
         Abstract | Full Text:  HTML   PDF

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  Supplement 24, December 1997

Unit 3.13 Production of T Cell Clones
         Full Text:  HTML   PDF

Unit 3.14 Production of Mouse T Cell Hybridomas
         Abstract | Full Text:  HTML   PDF

Unit 10.20 Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
         Abstract | Full Text:  HTML   PDF

Unit 10.21 Quantitation of Rare DNAs by PCR
         Abstract | Full Text:  HTML   PDF

Unit 15.9 The NOD Mouse: A Model for Insulin-Dependent Diabetes Mellitus
         Abstract | Full Text:  HTML   PDF

Unit 17.1 Bacteriophage Library Construction and Selection of Recombinant Antibodies

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  Supplement 23, September 1997

Unit 6.1 Measurement of Lymphokine Receptors
         Abstract | Full Text:  HTML   PDF

Unit 7.32 Isolation and Generation of Human Dendritic Cells
         Abstract | Full Text:  HTML   PDF

Unit 16.1 Choosing and Preparing Antigen-Presenting Cells
         Full Text:  HTML   PDF

Unit 16.2 Presenting Exogenous Antigen to T Cells
         Full Text:  HTML   PDF

Appendix 1J Key Molecules Involved in Receptor-Mediated Lymphocyte Activation

Appendix 3O Gram Staining
         Abstract | Full Text:  HTML   PDF

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  Supplement 22, June 1997

Unit 3.20 Isolation of Mouse Neutrophils
         Abstract | Full Text:  HTML   PDF

Unit 3.21 Isolation of Mouse Intrahepatic Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 6.23 Measurement of Interleukin 16
         Abstract | Full Text:  HTML   PDF

Unit 9.7 Synthesis of Multiple Peptides on Plastic Pins
         Abstract | Full Text:  HTML   PDF

Unit 10.27 Molecular Analysis of Mouse T Cell Receptor Expression Using PCR
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Using Protein Kinase and Protein Phosphatase Inhibitors to Dissect Protein Phosphorylation Pathways
         Abstract | Full Text:  HTML   PDF

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  Supplement 21, March 1997

Unit 2.7 Purification of Immunoglobulin G
         Abstract | Full Text:  HTML   PDF

Unit 2.8 Fragmentation of Immunoglobulin G
         Abstract | Full Text:  HTML   PDF

Unit 2.9 Purification of Immunoglobulin M and Immunoglobulin D
         Full Text:  HTML   PDF

Unit 2.10 Fragmentation of Immunoglobin M

Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity
         Full Text:  HTML   PDF

Unit 15.8 Experimental Autoimmune Myasthenia Gravis in the Mouse
         Abstract | Full Text:  HTML   PDF

Appendix 3A Monitoring Cell Growth
         Abstract | Full Text:  HTML   PDF

Appendix 3B Trypan Blue Exclusion Test of Cell Viability
         Abstract | Full Text:  HTML   PDF

Appendix 3C Wright-Giemsa and Nonspecific Esterase Staining of Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3D Labeling Cells in Microtiter Plates for Determination of [ 3 H]Thymidine Uptake
         Abstract | Full Text:  HTML   PDF

Appendix 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
         Abstract | Full Text:  HTML   PDF

Appendix 3F Obtaining Human Peripheral Blood Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3G Cryopreservation of Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3H Dialysis and Concentration of Protein Solutions
         Abstract | Full Text:  HTML   PDF

Appendix 3I Column Chromatography of Antibodies and Fragments
         Abstract |Full Text:  HTML   PDF

Appendix 3K Silanizing Glassware
         Abstract |Full Text:  HTML   PDF

Appendix 3L Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
         Abstract |Full Text:  HTML   PDF

Appendix 3M Preparing Frozen Bacterial Stocks
         Abstract |Full Text:  HTML   PDF

Appendix 3N Transformation of E. coli by Electroporation
         Abstract |Full Text:  HTML   PDF

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  Supplement 20, December 1996

Unit 5.8 Immunohistochemistry

Unit 7.31 Isolation of Human Eosinophils
         Abstract | Full Text:  HTML   PDF

Unit 11.6 Production of Antibodies that Recognize Specific Tyrosine-Phosphorylated Peptides

Unit 15.5 Collagen-Induced Arthritis
         Full Text:  HTML   PDF

Unit 15.6 Experimental Autoimmune Uveoretinitis in the Rat and Mouse

Unit 15.7 Experimental Autoimmune Thyroiditis in the Mouse
         Full Text:  HTML   PDF

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  Supplement 19, September 1996

Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
         Full Text:  HTML   PDF

Unit 7.30 Isolation of Human Intestinal Mucosal Mononuclear Cells
         Abstract | Full Text:  HTML   PDF

Unit 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
         Full Text:  HTML   PDF

Unit 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
         Full Text:  HTML   PDF

Unit 15.3 The BB Rat as a Model of Human Insulin-Dependent Diabetes Mellitus
         Abstract | Full Text:  HTML   PDF

Unit 15.4 Adjuvant Arthritis in the Rat
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, June 1996

Unit 2.9 Purification of Immunoglobulin M and Immunoglobulin D

Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF, Erythropoietin, Steel Factor, and Flt-3 Ligand
         Abstract | Full Text:  HTML   PDF

Unit 6.13 Measurement of Mouse and Human Interleukin 9

Unit 6.14 Measurement of Mouse and Human Interleukin 10
         Abstract | Full Text:  HTML   PDF

Unit 6.15 Measurement of Human Interleukin 11
         Abstract | Full Text:  HTML   PDF

Unit 6.22 Measurement of Interleukin 15
         Abstract | Full Text:  HTML   PDF

Unit 8.2 Immunoaffinity Chromatography
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, March 1996

Unit 6.6 Measurement of Interleukin 6
         Abstract | Full Text:  HTML   PDF

Unit 6.7 Measurement of Interleukin 7
         Abstract | Full Text:  HTML   PDF

Unit 7.7 Isolation of Human NK Cells and Generation of LAK Activity
         Abstract | Full Text:  HTML   PDF

Unit 7.14 Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
         Abstract | Full Text:  HTML   PDF

Unit 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
         Abstract | Full Text:  HTML   PDF

Unit 7.18 Measurement of Cytotoxic Activity of NK/LAK Cells
         Abstract | Full Text:  HTML   PDF

Appendix 1A Abbreviations Used in this Manual
         Abstract | Full Text:  HTML   PDF

Appendix 1B Amino Acids
         Abstract | Full Text:  HTML   PDF

Appendix 1C Commonly Used Mouse Strains
         Abstract | Full Text:  HTML   PDF

Appendix 1D Commonly Used Detergents
         Abstract | Full Text:  HTML   PDF

Appendix 1E Murine Models of Immunodeficiency and Autoimmune Disease
         Abstract | Full Text:  HTML   PDF

Appendix 1F Mouse Endogenous Superantigens: Ms and Mls-like Determinants Encoded by Mouse Retroviruses
         Abstract | Full Text:  HTML   PDF

Appendix 1G HLA Type of EBV-Transformed Human B Cell Lines

Appendix 1H Commonly Used Rat Strains
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, December 1995

Unit 2.11 Preparation of Immune Stimulating Complexes (ISCOMs) as Adjuvants
         Abstract | Full Text:  HTML   PDF

Unit 3.17 Morphological, Biochemical, and Flow Cytometric Assays of Apoptosis

Unit 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
         Full Text:  HTML   PDF

Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry

Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan

Unit 7.5 Isolation of B Cell Populations

Appendix 1I Peptide Binding Motifs for MHC Class I and II Molecules

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  Supplement 15, September 1995

Unit 1.7 Blood Collection
         Full Text:  HTML   PDF

Unit 1.8 Euthanasia
         Full Text:  HTML   PDF

Unit 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes, Peyer's Patch, and Lamina Propria Cells
         Abstract | Full Text:  HTML   PDF

Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4
         Abstract | Full Text:  HTML   PDF

Unit 6.16 Measurement of Human and Mouse Interleukin-12
         Abstract | Full Text:  HTML   PDF

Unit 6.21 Detection of Cytokine Receptors by Flow Cytometry

Unit 7.29 Cell Adhesion Under Hydrodynamic Flow Conditions
         Abstract | Full Text:  HTML   PDF

Appendix 4A Monoclonal Antibodies to Human Cell Surface Antigens

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  Supplement 14, June 1995

Unit 1.1 Managing Immunocompromised Animals
         Full Text:  HTML   PDF

Unit 1.2 Managing Immunocompromised Animals
         Full Text:  HTML   PDF

Unit 1.3 Handling and Restraint
         Full Text:  HTML   PDF

Unit 1.4 Anesthesia

Unit 1.5 Animal Identification
         Full Text:  HTML   PDF

Unit 1.6 Parenteral Injections
         Full Text:  HTML   PDF

Unit 2.13 Production of Bispecific Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry
         Full Text:  HTML   PDF

Unit 10.26 Evaluating Human T Cell Receptor Gene Expression by PCR
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, March 1995

Unit 2.4 Production of Polyclonal Antisera
         Full Text:  HTML   PDF

Unit 6.12 Measurement of α and β Chemokines

Unit 6.20 Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 8.16 Biotinylation and Analysis of Membrane-Bound and Soluble Proteins
         Abstract | Full Text:  HTML   PDF

Unit 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
         Full Text:  HTML   PDF

Unit 14.9 Measurement of Interferon-Mediated Antiviral Activity of Macrophages
         Abstract | Full Text:  HTML   PDF

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  Supplement 12, December 1994

Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions

Unit 10.17A Stable Transfer of Genes into Mammalian Cells
         Abstract | Full Text:  HTML   PDF

Unit 10.17B Transfection of Lymphoid Cells
         Abstract | Full Text:  HTML   PDF

Unit 10.25 Double-Stranded DNA Sequence Analysis
         Abstract | Full Text:  HTML   PDF

Unit 14.5 Oxidative Metabolism of Murine Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.6 Measurement of Bacterial Ingestion and Killing by Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.7 Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity
         Abstract | Full Text:  HTML   PDF

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  Supplement 11, September 1994

Unit 7.28 Measurement of Cellular Adhesion Under Static Conditions
         Abstract | Full Text:  HTML   PDF

Unit 13.5 Isolation and Functional Assay of the Membrane Complement Inhibitors CD55 (DAF) and CD59 (MIRL)
         Abstract | Full Text:  HTML   PDF

Unit 14.1 Isolation of Murine Macrophages
         Full Text:  HTML   PDF

Unit 14.2 Characterization of Murine Macrophages
         Full Text:  HTML   PDF

Unit 14.3 Characterization of Human Monocytes/Macrophages
         Abstract | Full Text:  HTML   PDF

Unit 14.4 Activation of Murine Macrophages
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, June 1994

Unit 4.6 Assessment of Lymphocyte Development in Radiation Bone Marrow Chimeras
         Full Text:  HTML   PDF

Unit 6.18 Measurement of IL-13

Unit 6.19 ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
         Full Text:  HTML   PDF

Unit 9.7 Synthesis of Peptides Immobilized on Polypropylene Pins

Unit 9.8 Use of Synthetic Peptide Combinatorial Libraries

Unit 13.3 Isolation and Purification of C3 from Human Plasma
         Abstract | Full Text:  HTML   PDF

Unit 13.4 Assays for Membrane Complement Receptors
         Abstract | Full Text:  HTML   PDF

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  Supplement 9, March 1994

Unit 8.13 Metabolic Radiolabeling of Animal Cell Glycoconjugates
         Abstract | Full Text:  HTML   PDF

Unit 8.14 Inhibition of N-Linked Glycosylation
         Abstract | Full Text:  HTML   PDF

Unit 8.15 Release of Saccharides from Glycoconjugates
         Full Text:  HTML   PDF

Unit 13.1 Classical Pathway Evaluation
         Abstract | Full Text:  HTML   PDF

Unit 13.2 Alternative Pathway Evaluation
         Abstract | Full Text:  HTML   PDF

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  Supplement 8, December 1993

Unit 3.5B Isolation of Ly-1/CD5 B Cells by Cell Sorting

Unit 7.26 Measuring Degranulation of Mast Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.27 51 Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
         Abstract | Full Text:  HTML   PDF

Unit 10.1 Purification and Concentration of DNA from Aqueous Solutions
         Abstract | Full Text:  HTML   PDF

Unit 10.5 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels
         Abstract | Full Text:  HTML   PDF

Unit 10.24 cDNA Amplification Using One-Sided (Anchored) PCR
         Abstract | Full Text:  HTML   PDF

Unit 12.9 In Vitro Evaluation of Experimental Agents for Anti-HIV Activity
         Abstract | Full Text:  HTML   PDF

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  Supplement 7, September 1993

Unit 9.6 Synthesis and Use of MAP Peptides

Unit 10.12 Analysis of RNA by Northern and Slot-Blot Hybridization
         Abstract | Full Text:  HTML   PDF

Unit 11.4 Immune-Complex Assays for Tyrosine Protein Kinases
         Abstract | Full Text:  HTML   PDF

Unit 11.5 Assessment of the Effects of Tyrosine Protein Kinase Inhibitors
         Abstract | Full Text:  HTML   PDF

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  Supplement 6, June 1993

Unit 3.19 Isolation of Mouse Small Intestinal Intraepithelial Lymphocytes

Unit 4.5 Delayed-Type Hypersensitivity
         Abstract | Full Text:  HTML   PDF

Unit 7.23 Isolation and Functional Analysis of Neutrophils
         Abstract | Full Text:  HTML   PDF

Unit 7.24 Isolation of Human Basophils
         Abstract | Full Text:  HTML   PDF

Unit 8.10 Immunoblotting and Immunodetection

Unit 10.6A Southern Blotting
         Abstract | Full Text:  HTML   PDF

Unit 10.6B Hybridization Analysis of DNA Blots
         Abstract | Full Text:  HTML   PDF

Unit 12.8 In Situ Hybridization for Detection of HIV RNA
         Abstract | Full Text:  HTML   PDF

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  Supplement 5, March 1993

Unit 12.1 General Guidelines for Experimenting with HIV

Unit 12.2 Isolation and Quantitation of HIV in Peripheral Blood
         Abstract | Full Text:  HTML   PDF

Unit 12.3 Infection of CD4 Primary Cell Lines and Induction of HIV
         Full Text:  HTML

Unit 12.4 Culture of HIV in Monocytes and Macrophages

Unit 12.5 Detection Assays for HIV Proteins

Unit 12.6 Detection of HIV DNA and RNA Using PCR

Unit 12.7 Regulation of the HIV Promoter/Enhancer

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  Supplement 4, December 1992

Unit 2.11 Preparation of Immune Stimulating Complexes (ISCOMs) as Adjuvants

Unit 3.18 Fetal Thymus Organ Culture for T Cell Development Studies
         Full Text:  HTML   PDF

Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan

Unit 6.16 Measurement of Human and Mouse Interleukin 12

Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand)
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Isolation of Proteins for Microsequence Analysis
         Full Text:  HTML   PDF

Unit 9.5 Use of Bromoacetylation for Preparing Conformationally Constrained Cyclic and Polymeric Peptides and Peptide-Carrier Conjugates

Unit 10.20 Enzymatic Amplification of DNA by PCR

Unit 10.21 Quantitation of Rare DNAs by PCR

Unit 10.22 Detection of Isotype Switch Rearrangement in Bulk Culture by PCR
         Abstract | Full Text:  HTML   PDF

Unit 10.23 Detection of Cytokine mRNA Expression by PCR
         Abstract | Full Text:  HTML   PDF

Appendix 1 Abbreviations and Useful Data

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  Supplement 3, September 1992

Unit 3.7 Enrichment of Dendritic Cells

Unit 3.17 Morphological and Biochemical Assays of Apoptosis

Unit 10.12 Analysis of RNA by Northern and Slot-Blot Hybridization

Unit 10.13 Calcium Phosphate Transfection
         Abstract | Full Text:  HTML   PDF

Unit 10.14 Transfection Using DEAE-Dextran
         Abstract | Full Text:  HTML   PDF

Unit 10.15 Transfection by Electroporation
         Abstract | Full Text:  HTML   PDF

Unit 10.16 Liposome-Mediated Transfection
         Abstract | Full Text:  HTML   PDF

Unit 10.17 Use of Monoclonal Antibodies for Expression Cloning

Unit 10.18 Use of Monoclonal Antibodies for Expression Cloning
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Analysis of Inositol Phospholipid Turnover During Lymphocyte Activation
         Full Text:  HTML   PDF

Unit 11.2 Preparation and Analysis of Phosphorylated Proteins
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Antiphosphotyrosine Blotting
         Full Text:  HTML   PDF

Appendix 1 Abbreviations and Useful Data

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  Supplement 2, June 1992

Unit 1.10 Survival Surgery: Removal of the Spleen or Thymus
         Abstract | Full Text:  HTML   PDF

Unit 4.4 Skin Allograft Rejection
         Full Text:  HTML   PDF

Unit 6.13 Measurement of Mouse and Human Interleukin 9

Unit 6.14 Measurement of Mouse and Human Interleukin 10

Unit 6.15 Measurement of Human Interleukin 11

Unit 10.1 Purification and Concentration of DNA from Aqueous Solutions

Unit 10.2 Preparation of Genomic DNA from Mammalian Tissue
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Preparation of Bacterial Plasmid DNA

Unit 10.4 Agarose Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 10.5 Isolation and Purification of Large DNA Restriction Fragments from Agarose Gels

Unit 10.6 Southern Hybridization Using Nylon Filters

Unit 10.7 Deprotection of Oligonucleotides and Purification Using Denaturing PAGE
         Abstract | Full Text:  HTML   PDF

Unit 10.8 Digestion of DNA with Restriction Endonucleases
         Abstract | Full Text:  HTML   PDF

Unit 10.9 Reagents and Radioisotopes Used to Manipulate Nucleic Acids
         Abstract | Full Text:  HTML   PDF

Unit 10.10 Enzymatic Labeling of Nucleic Acids
         Abstract | Full Text:  HTML   PDF

Appendix 3K Silinazing GlasswareAutoradiography

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  Supplement 1, March 1992

Unit 2.2 Isotype Determination of Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 2.9 Purification of Immunoglobulin M

Unit 2.10 Fragmentation of Immunoglobulin M

Unit 3.16 Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation

Unit 6.12 Measurement of Human Neutrophil Attractant Protein-1 (NAP-1; IL8)

Unit 7.23 Isolation and Functional Analysis of Neutrophils

Unit 7.24 Isolation of Human Basophils

Unit 7.25 Isolation of Tissue Mast Cells
         Abstract | Full Text:  HTML   PDF

Unit 9.1 Peptide Synthesis

Unit 9.2 Purification and Analysis of Peptides

Unit 9.3 Selection of Immunogenic Peptides for Antisera Production

Unit 9.4 Production of Antipeptide Antisera

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  Core Publication, 1991

Unit 1.1 Animal Health Assurance

Unit 1.2 Managing Immunocompromised Animals

Unit 1.3 Handling and Restraint

Unit 1.4 Anesthesia

Unit 1.5 Animal Identification

Unit 1.6 Parenteral Injections

Unit 1.7 Blood Collection

Unit 1.8 Euthenasia

Unit 1.9 Removal of Lymphoid Organs
         Abstract | Full Text:  HTML   PDF

Unit 2.1 Enzyme-Linked Immunosorbent Assays
         Abstract | Full Text:  HTML   PDF

Unit 2.2 Isotype Determination of Antibodies

Unit 2.3 Double-Immunodiffusion Assay for Detecting Specific Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 2.4 Production of Polyclonal Antisera in Rabbits, Rats, Mice, and Hamsters

Unit 2.5 Production of Monoclonal Antibodies
         Full Text:  HTML   PDF

Unit 2.6 Monoclonal Antibody Supernatant and Ascites Fluid Production

Unit 2.7 Purification of Immunoglobin G

Unit 2.8 Fragmentation of Immunoglobin G

Unit 3.1 Isolation of Mouse Mononuclear Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.2 T Cell Enrichment By Nonadherence to Nylon
         Abstract | Full Text:  HTML   PDF

Unit 3.3 T Cell Enrichment by Cytotoxic Elimination of B Cells and Accessory Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.4 T Cell Depletion by Cytotoxic Elimination
         Abstract | Full Text:  HTML   PDF

Unit 3.5 Fractionation of T Cells and B Cells Using Panning Techniques

Unit 3.6 Depletion of Accessory Cells by Adherence to Sephadex G-10
         Abstract | Full Text:  HTML   PDF

Unit 3.7 Enrichment of Accessory Cells by Glass Adherence and Radioresistance

Unit 3.8 In Vitro Antibody Production

Unit 3.9 Early Parameters of B Cell Activation

Unit 3.10 Proliferative Assays for B Cell Function
         Abstract | Full Text:  HTML   PDF

Unit 3.11 Induction and Measurement of Cytotoxic T Lymphocyte Activity

Unit 3.12 Proliferative Assay for T Cell Function

Unit 3.13 Production of T Cell Clones

Unit 3.14 Production of Mouse T Cell Hydridomas

Unit 3.15 Quantitation of Functional T Cells by Limiting Dilution
         Abstract | Full Text:  HTML   PDF

Unit 4.1 In Vivo Depletion of CD4- and CD8-Specific T Cells
         Abstract | Full Text:  HTML   PDF

Unit 4.2 Contact Hypersensitivity
         Abstract | Full Text:  HTML   PDF

Unit 4.3 Graft-versus-Host Disease

Unit 5.1 Overview of Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 5.2 Analysis of Flow Cytometry Data
         Abstract | Full Text:  HTML   PDF

Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry

Unit 5.4 Flow Cytometry Analysis Using the Becton Dickinson FACScan

Unit 5.5 Measurement of Intracellular Ions by Flow Cytometry

Unit 5.6 Measurement of Intercellular Conjugates by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 5.7 Use of Flow Cytometry for DNA Analysis

Unit 5.8 Identification of Cells in Tissue Sections

Unit 6.1 Measurement of Lymphokine Receptors

Unit 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1

Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4

Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Growth Factors

Unit 6.5 Measurement of Mouse and Human Interleukin 5
         Abstract | Full Text:  HTML   PDF

Unit 6.6 Measurement of Mouse and Human Interleukin 6

Unit 6.7 Measurement of Interleukin 7

Unit 6.8 Measurement of Mouse and Human Interferon γ
         Abstract | Full Text:  HTML   PDF

Unit 6.9 Measurement of Antiviral Activity Induced by Interferons α, β, and γ
         Abstract | Full Text:  HTML   PDF

Unit 6.10 Measurement of Tumor Necrosis Factor α and β
         Abstract | Full Text:  HTML   PDF

Unit 6.11 Measurement of Transforming Growth Factor β

Unit 7.1 Isolation of Whole Mononuclear Cells from Peripheral Blood

Unit 7.2 Isolation of T Cells Using Rosetting Procedures
         Abstract | Full Text:  HTML   PDF

Unit 7.3 Purification of T Cell Subpopulations
         Abstract | Full Text:  HTML   PDF

Unit 7.4 Immunomagnetic Purification of T Cell Subpopulations
         Text:  HTML   PDF

Unit 7.5 Isolation of B Cell Population

Unit 7.6 Isolation of Monocyte/Macrophage Populations
         Full Text:  HTML   PDF

Unit 7.7 Isolation of Cell Populations Containing NK Cells and LAK Precursor Cells

Unit 7.8 Isolation of Mononuclear Cells From Tonsillar Tissue
         Full Text:  HTML   PDF

Unit 7.9 Detection of Unseparated Human Lymphocytes by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.10 Measurement of Proliferative Responses of Cultured Lymphocytes
         Full Text:  HTML   PDF

Unit 7.11 Induction of Polyclonal Immunoglobulin Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 7.12 Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
         Abstract | Full Text:  HTML   PDF

Unit 7.13 Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
         Abstract | Full Text:  HTML   PDF

Unit 7.14 Measurement of Polyclonal Immunoglobin Synthesis Using the ELISPOT Assay

Unit 7.15 Measurement of In Vitro Specific-Antibody Synthesis
         Abstract | Full Text:  HTML   PDF

Unit 7.16 Induction and Measurement of In Vivo Antibody Responses
         Abstract | Full Text:  HTML   PDF

Unit 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function

Unit 7.18 Measurement of Cytotoxic NK/LAK Cells

Unit 7.19 Generation and Maintenance of T Cell Lines and Clones

Unit 7.20 Generation of HTLV-I-Transformed T Cell Lines

Unit 7.21 Generation of T Cell Hybridomas
         Full Text:  HTML   PDF

Unit 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
         Full Text:  HTML   PDF

Unit 8.1 Solubilization of Lymphocytes

Unit 8.2 Immunoaffinity Chromatography

Unit 8.3 Immunoprecipitation

Unit 8.4 One-Dimensional Gel Electrophoresis of Proteins
         Full Text:  HTML

Unit 8.5 Two-Dimensional Gel Electrophoresis Using the O'Farrell System
         Full Text:  HTML   PDF

Unit 8.6 Diagonal Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Isolation of Proteins for Microsequence Analysis

Unit 8.8 Electroelution of Proteins from Stained Gels
         Abstract | Full Text:  HTML   PDF

Unit 8.9 Staining Proteins in Gels

Unit 8.10 Immunoblotting

Unit 8.11 Iodination of Soluble and Membrane-Bound Proteins
         Abstract | Full Text:  HTML   PDF

Unit 8.12 Biosynthetic Labeling of Proteins
         Abstract | Full Text:  HTML   PDF

Appendix 1 Abbreviations and Useful Data

Appendix 2 Commonly Used Media, Buffers, and Stock Solutions

Appendix 3A Monitoring Cell Growth

Appendix 3B Trypan Blue Exclusion Test of Cell Viability

Appendix 3C Wright-Giemsa and Nonspecific Esterase Staining of Cells

Appendix 3D Labeling Cells in Microtiter Plates for Determination of [ 3 H]Thymidine Uptake

Appendix 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures

Appendix 3F Obtaining Human Peripheral Blood Cells

Appendix 3G Cryopreservation of Cells

Appendix 3H Dialysis and Concentration of Protein Solutions

Appendix 3I Column Chromatography of Antibodies and Fragments

Appendix 3J Autoradiography

Appendix 4 The CD System of Leukocyte Surface Molecules

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