Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

Mission Statement

Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.

Current Protocols in Immunology is the comprehensive resource for protocols used to study the cellular, sub-cellular, genetic, and molecular properties of the immune system. From basic methods used to generate and measure antibody and cell-mediated responses to experimental strategies and current approaches to studying complex immunoregulatory properties of lymphoid cell populations, Current Protocols in Immunology offers step-by-step instructions written by experts from throughout the world. The publication includes methods that employ animal models to investigate the unanswered questions related to the physiology of the immune system as well as the nature of immune-based diseases and host responses to pathogens. It also includes an extensive number of protocols that utilize human-derived cell populations and tissues, and much more.

The target audience for Current Protocols in Immunology is anyone who is interested in studying basic and pathophysiologic features of the immune system as well as the dynamics of host-pathogen interactions. Investigators working in disciplines other than immunology will also find the methods described in Current Protocols in Immunology useful because of the many applications of sensitive immune-based assays used to investigate questions in their fields of interest. No prior experience in the field of immunology is assumed. Thus, protocols are written in sufficient detail so that readers can easily follow and perform the analyses described in Current Protocols in Immunology on their own, making it the ideal companion for experimentalists in all biological disciplines.


immunologists • cell biologists • molecular biologists • biologists • geneticists • systems biologists • developmental biologists


immunology, antibodies, B-lymphocytes, T-lymphocytes, NK cells, phagocytes, innate immunity, antigen-presentation, autoimmunity, immunodeficiency, antigen-processing, cytokines, chemokines, genes, laboratory protocols, Current Protocols