Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Cell Culture
    1. Bacteria
    2. Mammalian
      1. Cryopreservation and Thawing of Cells
      2. Depletion of Accessory Cells by Adherence to Sephadex G-10
      3. Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay
      4. Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
      5. Fractionation of T and B Cells Using Magnetic Beads
      6. HLA Type of EBV-Transformed Human B Cell Lines
      7. In Vitro Systems for the Study of T Cell Development: Fetal Thymus Organ Culture and OP9-DL1 Cell Coculture
      8. Isolation of Dendritic Cells
      9. Isolation of Ly-1+/CD5+ B Cells by Cell Sorting
      10. Isolation of Mouse Intrahepatic Lymphocytes
      11. Isolation of Mouse Mononuclear Cells
      12. Isolation of Mouse Neutrophils
      13. Labeling Cells in Microtiter Plates for Determination of [3H]Thymidine Uptake
      14. Large-Scale Cell Culture
      15. Generation, Isolation, and Maintenance of Rodent Mast Cells and Mast Cell Lines
      16. Monitoring Cell Growth
      17. Obtaining Human Peripheral Blood Cells
      18. Production of TH1 and TH2 Cell Lines and Clones
      19. T Cell Depletion by Cytotoxic Elimination
      20. T Cell Enrichment by Cytotoxic Elimination of B Cells and Accessory Cells
      21. T Cell Enrichment By Nonadherence to Nylon
      22. The Isolation and Characterization of Murine Macrophages
      23. Trypan Blue Exclusion Test of Cell Viability
      24. Wright-Giemsa and Nonspecific Esterase Staining of Cells
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Immunology
  11. Intermolecular Interactions
  12. Laboratory Organisms and Animal Models
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Neuroscience
  17. Preparation and Analysis of DNA and RNA
  18. Protein Production, Purification, and Analysis
  19. Proteomics
  20. RNA
  21. Safety
  22. Stem Cells
  23. Structural Analysis of Biomolecules
  24. Supporting Lab Techniques