Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Cell Culture
  5. Cytometry
  6. Gene Expression
  7. Genetics and Genomics
  8. Imaging and Microscopy
  9. Immunology
  10. Immunology
    1. Adjuvants
    2. Animal Models
    3. Animal Models
    4. Antigen Presentation
    5. Antigen Processing
    6. Antigens
    7. Autoimmunity
    8. Cell Activation
    9. Complement
    10. Cytokines
    11. Detection and Analysis of HIV
    12. Engineering Immune Molecules and Receptors
    13. Flow Cytometry
    14. Hematopoiesis
    15. Host-Pathogen Interactions
    16. Immune Cell Function
    17. Immune Disease
      1. 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
      2. Assays for B Cell and Germinal Center Development
      3. Evaluation of B Cell Function in Patients with HIV
      4. Cell Adhesion Under Hydrodynamic Flow Conditions
      5. Culture of HIV in Monocytes and Macrophages
      6. Detection Assays for HIV Proteins
      7. Detection of HIV DNA and RNA Using PCR
      8. Detection of Unseparated Human Lymphocytes by Flow Cytometry
      9. Evaluating Neutralizing Antibodies Against HIV, SIV, and SHIV in Luciferase Reporter Gene Assays
      10. General Guidelines for Experimenting with HIV
      11. Generation and Maintenance of Cloned Human T Cell Lines
      12. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
      13. Generation of HTLV-I-Transformed T Cell Lines
      14. Infection of CD4+ Primary T Cells and Cell Lines, Generation of Chronically Infected Cell Lines, and Induction of HIV Expression
      15. In Vitro Models of Human T Cell Anergy
      16. Herpesvirus saimiri Transformation of Human T Lymphocytes
      17. Immunomagnetic Purification of T Cell Subpopulations
      18. Immunophenotyping of T Cell Subpopulations in HIV Disease
      19. In Situ Hybridization for Detection of HIV RNA
      20. In Vitro Evaluation of Experimental Agents for Anti-HIV Activity
      21. Induction and Measurement of In Vivo Antibody Responses
      22. Induction of Polyclonal Immunoglobulin Synthesis
      23. Isolation and Purification of Human Intestinal Macrophages
      24. The Purification and Functional Analysis of Human CD4+CD25high Regulatory T Cells
      25. Isolation and Characterization of Human Natural Killer Cell Subsets
      26. Isolation and Functional Analysis of Neutrophils
      27. Isolation and Quantitation of HIV in Peripheral Blood
      28. Isolation of Human Basophils
      29. Isolation of Human Intestinal Mucosal Mononuclear Cells
      30. Isolation of Human NK Cells and Generation of LAK Activity
      31. Isolation of Mononuclear Cells from Tonsillar Tissue
      32. Isolation of T Cells Using Rosetting Procedures
      33. Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
      34. Measurement of Cellular Adhesion Under Static Conditions
      35. Measurement of Cytokine Production Using Whole Blood
      36. Measurement of Cytotoxic Activity of NK/LAK Cells
      37. Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
      38. Measurement of In Vitro Specific-Antibody Synthesis
      39. Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
      40. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
      41. Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
      42. Measurement of Prostaglandins and Other Eicosanoids
      43. Measuring Degranulation of Mast Cells
      44. Isolation of Human Monocyte Populations
      45. Purification of T Cell Subpopulations
      46. Regulation of the HIV Promoter/Enhancer
      47. Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors
    18. Immunization
    19. Immunofluorescence and Cell Sorting
    20. Immunologic Study in Humans
    21. In Vitro Assays for Immune Cell Function
    22. In Vitro Assays for Lymphocyte Function
    23. In Vivo Assays for Immune Cell Function
    24. In Vivo Assays for Lymphocyte Function
    25. Induction of Immune Responses
    26. Inflammatory Disease Models
    27. Innate Immunity
    28. Ligand-Receptor Interactions
    29. Major Histocompatibility
    30. Models of Autoimmune and Inflammatory Disease
    31. Models of Pulmonary Disease
    32. Regulation of Immune Responses
    33. Stem and Progenitor Cells
    34. Toll-Like Receptors
    35. Tumor immunology
  11. Intermolecular Interactions
  12. Laboratory Organisms and Animal Models
  13. Microbiology
  14. Molecular Biology
  15. Molecular Biology
  16. Neuroscience
  17. Preparation and Analysis of DNA and RNA
  18. Protein Production, Purification, and Analysis
  19. Proteomics
  20. RNA
  21. Safety
  22. Stem Cells
  23. Structural Analysis of Biomolecules
  24. Supporting Lab Techniques