Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture
  6. Cytometry
  7. Gene Expression
  8. Gene Expression
  9. Genetics and Genomics
  10. Imaging and Microscopy
  11. Immunization
  12. Immunology
  13. Immunology
  14. Immunology
    1. Adjuvants
    2. Animal Models
    3. Animal Models
    4. Antigen Presentation
    5. Antigen Processing
    6. Antigens
    7. Autoimmunity
    8. Cell Activation
    9. Complement
    10. Cytokines
    11. Detection and Analysis of HIV
    12. Engineering Immune Molecules and Receptors
    13. Flow Cytometry
    14. Hematopoiesis
    15. Host-Pathogen Interactions
    16. Immune Cell Function
    17. Immune Disease
    18. Immunization
    19. Immunofluorescence and Cell Sorting
    20. Immunologic Study in Humans
      1. 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
      2. A Practical Guide to Culturing Mouse and Human Bone Marrow Stromal Cells
      3. A Real-Time Cytotoxicity Assay as an Alternative to the Standard Chromium-51 Release Assay for Measurement of Human NK and T Cell Cytotoxic Activity
      4. Assays for B Cell and Germinal Center Development
      5. Cell Adhesion Under Hydrodynamic Flow Conditions
      6. Culture of HIV in Monocytes and Macrophages
      7. Differentiation and Characterization of Dendritic Cells from Human Embryonic Stem Cells
      8. Detection Assays for HIV Proteins
      9. Detection of Cytokine Receptors by Flow Cytometry
      10. Detection of Unseparated Human Lymphocytes by Flow Cytometry
      11. Differentiation of Mast Cells from Embryonic Stem Cells
      12. ELISPOT Assay for Measurement of Antigen-Specific and Polyclonal Antibody Responses
      13. Analysis of Gene Expression and Gene Silencing in Human Macrophages
      14. General Guidelines for Experimenting with HIV
      15. Generation and Maintenance of Cloned Human T Cell Lines
      16. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
      17. Generation of HTLV-I-Transformed T Cell Lines
      18. Infection of CD4+ Primary T Cells and Cell Lines, Generation of Chronically Infected Cell Lines, and Induction of HIV Expression
      19. Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines Derived from Peripheral Blood or Cord Blood
      20. Isolation and Functional Use of Human NKT Cells
      21. In Vitro Models of Human T Cell Anergy
      22. Creation of “Humanized” Mice to Study Human Immunity
      23. Herpesvirus saimiri Transformation of Human T Lymphocytes
      24. Immortalization of Human and Rhesus Macaque Primary Antigen-Specific T Cells by Retrovirally Transduced Telomerase Reverse Transcriptase
      25. Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies
      26. Immunomagnetic Purification of T Cell Subpopulations
      27. Induction and Measurement of In Vivo Antibody Responses
      28. Induction and Quantification of NETosis
      29. Induction of Polyclonal Immunoglobulin Synthesis
      30. Isolation and Purification of Human Intestinal Macrophages
      31. The Purification and Functional Analysis of Human CD4+CD25high Regulatory T Cells
      32. Isolation and Characterization of Human Natural Killer Cell Subsets
      33. Isolation and Functional Analysis of Human Neutrophils
      34. Isolation and Generation of Human Dendritic Cells
      35. Isolation and Quantitation of HIV in Peripheral Blood
      36. Isolation of Cells from the Feto-Maternal Interface
      37. Isolation of Human B Cell Populations
      38. Isolation of Human Basophils
      39. Isolation of Human Eosinophils
      40. Isolation of Human Intestinal Mucosal Mononuclear Cells
      41. Isolation of Human NK Cells and Generation of LAK Activity
      42. Isolation of Mononuclear Cells from Tonsillar Tissue
      43. Isolation of T Cells Using Rosetting Procedures
      44. Isolation of Tissue Mast Cells
      45. Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
      46. Measurement of Cellular Adhesion Under Static Conditions
      47. Measurement of Cytokine Production Using Whole Blood
      48. Measurement of Cytotoxic Activity of NK/LAK Cells
      49. Measurement of In Vitro Specific-Antibody Synthesis
      50. Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
      51. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
      52. Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
      53. Measurement of Proliferative Responses of Cultured Lymphocytes
      54. Measurement of Prostaglandins and Other Eicosanoids
      55. Measuring Degranulation of Mast Cells
      56. Models to Study NK Cell Biology and Possible Clinical Application
      57. Isolation of Human Monocyte Populations
      58. Purification of T Cell Subpopulations
      59. Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis
      60. Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR
      61. Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest
      62. Universal Precautions: Necessary Safety Procedures When Handling Human Blood, Body Fluids, and Specimens
      63. Venipuncture Procedures: Sources of Human Peripheral Blood Cells, Serum, and Plasma
    21. In Vitro Assays for Immune Cell Function
    22. In Vitro Assays for Lymphocyte Function
    23. In Vivo Assays for Immune Cell Function
    24. In Vivo Assays for Lymphocyte Function
    25. Induction of Immune Responses
    26. Inflammatory Disease Models
    27. Innate Immunity
    28. Ligand-Receptor Interactions
    29. Major Histocompatibility
    30. Models of Autoimmune and Inflammatory Disease
    31. Models of Pulmonary Disease
    32. Molecular Immunology
    33. Regulation of Immune Responses
    34. Stem and Progenitor Cells
    35. Toll-Like Receptors
    36. Tumor immunology
  15. Intermolecular Interactions
  16. Laboratory Organisms and Animal Models
  17. Microbiology
  18. Molecular Biology
  19. Molecular Biology
  20. Neuroscience
  21. Preparation and Analysis of DNA and RNA
  22. Protein Production, Purification, and Analysis
  23. Proteomics
  24. RNA
  25. Safety
  26. Stem Cells
  27. Structural Analysis of Biomolecules
  28. Supporting Lab Techniques