Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture
  6. Cytometry
  7. Gene Expression
  8. Genetics and Genomics
  9. Imaging and Microscopy
  10. Immunology
  11. Immunology
  12. Immunology
    1. Adjuvants
    2. Animal Models
    3. Animal Models
    4. Antigen Presentation
    5. Antigen Processing
    6. Antigens
    7. Autoimmunity
    8. Cell Activation
    9. Complement
    10. Cytokines
    11. Detection and Analysis of HIV
    12. Engineering Immune Molecules and Receptors
    13. Flow Cytometry
    14. Hematopoiesis
    15. Host-Pathogen Interactions
    16. Immune Cell Function
    17. Immune Disease
    18. Immunization
    19. Immunofluorescence and Cell Sorting
    20. Immunologic Study in Humans
      1. 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
      2. A Practical Guide to Culturing Mouse and Human Bone Marrow Stromal Cells
      3. Assays for B Cell and Germinal Center Development
      4. Cell Adhesion Under Hydrodynamic Flow Conditions
      5. Culture of HIV in Monocytes and Macrophages
      6. Differentiation and Characterization of Dendritic Cells from Human Embryonic Stem Cells
      7. Detection Assays for HIV Proteins
      8. Detection of Cytokine Receptors by Flow Cytometry
      9. Detection of Unseparated Human Lymphocytes by Flow Cytometry
      10. Differentiation of Mast Cells from Embryonic Stem Cells
      11. ELISPOT Assay for Measurement of Antigen-Specific and Polyclonal Antibody Responses
      12. Analysis of Gene Expression and Gene Silencing in Human Macrophages
      13. General Guidelines for Experimenting with HIV
      14. Generation and Maintenance of Cloned Human T Cell Lines
      15. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
      16. Generation of HTLV-I-Transformed T Cell Lines
      17. Infection of CD4+ Primary T Cells and Cell Lines, Generation of Chronically Infected Cell Lines, and Induction of HIV Expression
      18. Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines Derived from Peripheral Blood or Cord Blood
      19. Isolation and Functional Use of Human NKT Cells
      20. In Vitro Models of Human T Cell Anergy
      21. Creation of “Humanized” Mice to Study Human Immunity
      22. Herpesvirus saimiri Transformation of Human T Lymphocytes
      23. Immortalization of Human and Rhesus Macaque Primary Antigen-Specific T Cells by Retrovirally Transduced Telomerase Reverse Transcriptase
      24. Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies
      25. Immunomagnetic Purification of T Cell Subpopulations
      26. Induction and Measurement of In Vivo Antibody Responses
      27. Induction of Polyclonal Immunoglobulin Synthesis
      28. Isolation and Purification of Human Intestinal Macrophages
      29. The Purification and Functional Analysis of Human CD4+CD25high Regulatory T Cells
      30. Isolation and Characterization of Human Natural Killer Cell Subsets
      31. Isolation and Functional Analysis of Human Neutrophils
      32. Isolation and Generation of Human Dendritic Cells
      33. Isolation and Quantitation of HIV in Peripheral Blood
      34. Isolation of Cells from the Feto-Maternal Interface
      35. Isolation of Human B Cell Populations
      36. Isolation of Human Basophils
      37. Isolation of Human Eosinophils
      38. Isolation of Human Intestinal Mucosal Mononuclear Cells
      39. Isolation of Human NK Cells and Generation of LAK Activity
      40. Isolation of Mononuclear Cells from Tonsillar Tissue
      41. Isolation of T Cells Using Rosetting Procedures
      42. Isolation of Tissue Mast Cells
      43. Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
      44. Measurement of Cellular Adhesion Under Static Conditions
      45. Measurement of Cytokine Production Using Whole Blood
      46. Measurement of Cytotoxic Activity of NK/LAK Cells
      47. Measurement of In Vitro Specific-Antibody Synthesis
      48. Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
      49. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
      50. Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
      51. Measurement of Proliferative Responses of Cultured Lymphocytes
      52. Measurement of Prostaglandins and Other Eicosanoids
      53. Measuring Degranulation of Mast Cells
      54. Models to Study NK Cell Biology and Possible Clinical Application
      55. Isolation of Human Monocyte Populations
      56. Purification of T Cell Subpopulations
      57. Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis
      58. Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR
    21. In Vitro Assays for Immune Cell Function
    22. In Vitro Assays for Lymphocyte Function
    23. In Vivo Assays for Immune Cell Function
    24. In Vivo Assays for Lymphocyte Function
    25. Induction of Immune Responses
    26. Inflammatory Disease Models
    27. Innate Immunity
    28. Ligand-Receptor Interactions
    29. Major Histocompatibility
    30. Models of Autoimmune and Inflammatory Disease
    31. Models of Pulmonary Disease
    32. Molecular Immunology
    33. Regulation of Immune Responses
    34. Stem and Progenitor Cells
    35. Toll-Like Receptors
    36. Tumor immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Microbiology
  16. Molecular Biology
  17. Molecular Biology
  18. Neuroscience
  19. Preparation and Analysis of DNA and RNA
  20. Protein Production, Purification, and Analysis
  21. Proteomics
  22. RNA
  23. Safety
  24. Stem Cells
  25. Structural Analysis of Biomolecules
  26. Supporting Lab Techniques