Current Protocols in Immunology

Current Protocols in Immunology

Online ISBN: 9780471142737

DOI: 10.1002/0471142735

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  1. Bioinformatics
  2. Bioinformatics
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture
  6. Cytometry
  7. Gene Expression
  8. Gene Expression
  9. Genetics and Genomics
  10. Imaging and Microscopy
  11. Immunization
  12. Immunology
  13. Immunology
  14. Immunology
    1. Adjuvants
    2. Animal Models
    3. Animal Models
    4. Antigen Presentation
    5. Antigen Processing
    6. Antigens
    7. Autoimmunity
    8. Cell Activation
    9. Complement
    10. Cytokines
    11. Detection and Analysis of HIV
    12. Engineering Immune Molecules and Receptors
    13. Flow Cytometry
    14. Hematopoiesis
    15. Host-Pathogen Interactions
    16. Immune Cell Function
    17. Immune Disease
    18. Immunization
    19. Immunofluorescence and Cell Sorting
    20. Immunologic Study in Humans
    21. In Vitro Assays for Immune Cell Function
      1. 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC)
      2. A Practical Guide to Culturing Mouse and Human Bone Marrow Stromal Cells
      3. Activation of Murine Macrophages
      4. Analysis of Telomere Length and Telomerase Activity
      5. Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity
      6. Assays for B Lymphocyte Function
      7. Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces
      8. Cell Adhesion Under Hydrodynamic Flow Conditions
      9. Comparing Individual NK Cell Activity In Vitro
      10. Construction of Human-SCID Chimeric Mice
      11. Culture of HIV in Monocytes and Macrophages
      12. Culture, Expansion, and Differentiation of Murine Megakaryocytes from Fetal Liver, Bone Marrow, and Spleen
      13. Differentiation and Characterization of Dendritic Cells from Human Embryonic Stem Cells
      14. Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay
      15. Detection of Unseparated Human Lymphocytes by Flow Cytometry
      16. Early Events in B Lymphocyte Activation
      17. ELISPOT Assay to Detect Cytokine-Secreting Murine and Human Cells
      18. Evaluating Human T Cell Receptor Gene Expression by PCR
      19. Generation and Characterization of Mouse Basophils from Bone Marrow and Purification of Basophils from Spleen
      20. Generation and Maintenance of Cloned Human T Cell Lines
      21. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
      22. Generation of HTLV-I-Transformed T Cell Lines
      23. Granule Enzyme Exocytosis Assay for Cytotoxic T Lymphocyte Activation
      24. Isolation and Functional Use of Human NKT Cells
      25. In Vitro Models of Human T Cell Anergy
      26. Herpesvirus saimiri Transformation of Human T Lymphocytes
      27. Targeted Identification of Infection-Related HLA Class I–Presented Epitopes by Stable Isotope Tagging of Epitopes (SITE)
      28. Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP-Labeled Anti-Cytokine Antibodies
      29. In Situ Staining Using MHC Class I Tetramers
      30. In Vitro Systems for the Study of T Cell Development: Fetal Thymus Organ Culture and OP9-DL1 Cell Coculture
      31. Induction and Measurement of Cytotoxic T Lymphocyte Activity
      32. Induction and Measurement of In Vivo Antibody Responses
      33. Induction of Polyclonal Immunoglobulin Synthesis
      34. Isolation and Purification of Human Intestinal Macrophages
      35. Isolation and Analysis of Mouse Microglial Cells
      36. The Purification and Functional Analysis of Human CD4+CD25high Regulatory T Cells
      37. Isolation and Characterization of Human Natural Killer Cell Subsets
      38. Isolation and Functional Analysis of Human Neutrophils
      39. Isolation and Generation of Human Dendritic Cells
      40. Isolation of Cells from the Feto-Maternal Interface
      41. Isolation of Dendritic Cells
      42. Isolation of Human B Cell Populations
      43. Isolation of Human Basophils
      44. Isolation of Human Intestinal Mucosal Mononuclear Cells
      45. Isolation of Human NK Cells and Generation of LAK Activity
      46. Isolation of Mononuclear Cells from Tonsillar Tissue
      47. Isolation of Mouse Lymphocytes from Small Intestine Tissues
      48. Isolation of Mouse Neutrophils
      49. Isolation of T Cells Using Rosetting Procedures
      50. Isolation of Whole Mononuclear Cells from Peripheral Blood and Cord Blood
      51. Measurement of Bacterial Ingestion and Killing by Macrophages
      52. Measurement of Cellular Adhesion Under Static Conditions
      53. Measurement of Cytokine Production Using Whole Blood
      54. Measurement of Cytotoxic Activity of NK/LAK Cells
      55. Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
      56. Measurement of Human and Murine Interleukin 2 and Interleukin 4
      57. Measurement of In Vitro Specific-Antibody Synthesis
      58. Measurement of Intercellular Conjugates by Flow Cytometry
      59. Measurement of Interferon-Mediated Antiviral Activity of Macrophages
      60. Measurement of Interleukin 6
      61. Measurement of Interleukin-17
      62. Measurement of Interleukin-21
      63. Measurement of Intracellular Ions by Flow Cytometry
      64. Measurement of Mouse and Human Interleukin 5
      65. Measuring Opsonic Phagocytosis via Fcγ Receptors and Complement Receptors on Macrophages
      66. Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
      67. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
      68. Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique
      69. Measurement of Proliferative Responses of Cultured Lymphocytes
      70. Measurement of Prostaglandins and Other Eicosanoids
      71. Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay
      72. Measurement of Type I Interferon Production
      73. Measuring Apoptosis by Microscopy and Flow Cytometry
      74. Measuring Degranulation of Mast Cells
      75. Measuring Matrix Metalloproteinase Activity in Macrophages and Polymorphonuclear Leukocytes
      76. Measuring the Killing of Intracellular Pathogens: Leishmania
      77. Molecular Analysis of Mouse T Cell Receptor Expression Using PCR
      78. Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers
      79. Isolation of Human Monocyte Populations
      80. Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression
      81. Oxidative Metabolism of Murine Macrophages
      82. Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated by Phagocytes
      83. Measurement of Phagocytosis, Phagosome Acidification, and Intracellular Killing of Staphylococcus aureus
      84. Production of Monoclonal Antibodies
      85. Production of Mouse T Cell Hybridomas
      86. Production of TH1 and TH2 Cell Lines and Clones
      87. Proliferative Assays for B Cell Function
      88. Purification of T Cell Subpopulations
      89. Quantitation of Functional T Cells by Limiting Dilution
      90. Spectratype/Immunoscope Analysis of the Expressed TCR Repertoire
      91. Target-Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
      92. The Isolation and Characterization of Murine Macrophages
      93. Measurement of Tumor Cytolysis by Macrophages
      94. Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
      95. Use of Proteasome Inhibitors
    22. In Vitro Assays for Lymphocyte Function
    23. In Vivo Assays for Immune Cell Function
    24. In Vivo Assays for Lymphocyte Function
    25. Induction of Immune Responses
    26. Inflammatory Disease Models
    27. Innate Immunity
    28. Ligand-Receptor Interactions
    29. Major Histocompatibility
    30. Models of Autoimmune and Inflammatory Disease
    31. Models of Pulmonary Disease
    32. Molecular Immunology
    33. Regulation of Immune Responses
    34. Stem and Progenitor Cells
    35. Toll-Like Receptors
    36. Tumor immunology
  15. Intermolecular Interactions
  16. Laboratory Organisms and Animal Models
  17. Microbiology
  18. Molecular Biology
  19. Molecular Biology
  20. Neuroscience
  21. Preparation and Analysis of DNA and RNA
  22. Protein Production, Purification, and Analysis
  23. Proteomics
  24. RNA
  25. Safety
  26. Stem Cells
  27. Structural Analysis of Biomolecules
  28. Supporting Lab Techniques