Current Protocols in Cytometry

Current Protocols in Cytometry

Online ISBN: 9780471142959

DOI: 10.1002/0471142956

Archive


 

2014:    Supplement 70     Supplement 69     Supplement 68     Supplement 67    
2013:    Supplement 66     Supplement 65     Supplement 64     Supplement 63    
2012:    Supplement 62     Supplement 61     Supplement 60     Supplement 59    
2011:    Supplement 58     Supplement 57     Supplement 56     Supplement 55    
2010:    Supplement 54     Supplement 53     Supplement 52     Supplement 51     
2009:    Supplement 50     Supplement 49     Supplement 48     Supplement 47
2008:    Supplement 46     Supplement 45     Supplement 44     Supplement 43    
2007:    Supplement 42     Supplement 41     Supplement 40     Supplement 39    
2006:    Supplement 38     Supplement 37     Supplement 36     Supplement 35    
2005:    Supplement 34     Supplement 33     Supplement 32     Supplement 31    
2004:    Supplement 30     Supplement 29     Supplement 28     Supplement 27    
2003:    Supplement 26     Supplement 25     Supplement 24     Supplement 23    
2002:    Supplement 22     Supplement 21     Supplement 20     Supplement 19    
2001:    Supplement 18     Supplement 17     Supplement 16     Supplement 15    
2000:    Supplement 16     Supplement 13     Supplement 12     Supplement 11    
1999:    Supplement 10     Supplement 9       Supplement 8       Supplement 7      
1998:    Supplement 6       Supplement 5       Supplement 4       Supplement 3      
1997:    Supplement 2       Supplement 1      
1998:    Core publication




  Supplement 70, October 2014

Unit 2.23 FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.41 High-Resolution Cytometry for High-Content Cell Cycle Analysis
         Abstract | Full Text:  HTML   PDF

Unit 7.42 Confocal Microscopy for High-Resolution and High-Content Analysis of the Cell Cycle
         Abstract | Full Text:  HTML   PDF

Unit 12.36 Correlative Fluorescence and Electron Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 69, July 2014

Unit 8.14 Application of Flow-FISH for Dynamic Measurement of Telomere Length in Cell Division
         Abstract | Full Text:  HTML   PDF

Unit 9.47 Attenuation of Replication Stress–Induced Premature Cellular Senescence to Assess Anti-Aging Modalities
         Abstract | Full Text:  HTML   PDF

Unit 12.34 Imaging Autophagy
         Abstract | Full Text:  HTML   PDF

Unit 12.35 The Application of KillerRed for Acute Protein Inactivation in Living Cells
         Abstract | Full Text:  HTML   PDF

Unit 13.13 Application of the PrimRglo Assay Chemistry to Multiplexed Bead Assays
         Abstract | Full Text:  HTML   PDF

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  Supplement 68, April 2014

Unit 9.45 Measurement of Autophagy by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 9.46 Immunophenotyping of Paucicellular Samples
         Abstract | Full Text:  HTML   PDF

Unit 10.14 Intensity Calibration and Flat-Field Correction for Fluorescence Microscopes
         Abstract | Full Text:  HTML   PDF

Unit 12.33 A Rapid and Sensitive Automated Image-Based Approach for In Vitro and In Vivo Characterization of Cell Morphology and Quantification of Cell Number and Neurite Architecture
         Abstract | Full Text:  HTML   PDF

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  Supplement 67, January 2014

Unit 2.22 How to Build a Time-Gated Luminescence Microscope
         Abstract | Full Text:  HTML   PDF

Unit 9.43 Real-Time Detection of Protein Trafficking with High-Throughput Flow Cytometry (HTFC) and Fluorogen-Activating Protein (FAP) Base Biosensor
         Abstract | Full Text:  HTML   PDF

Unit 9.44 OpenSource Lab-on-a-Chip Physiometer for Accelerated Zebrafish Embryo Biotests
         Abstract | Full Text:  HTML   PDF

Unit 12.32 A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm
         Abstract | Full Text:  HTML   PDF

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  Supplement 66, October 2013

Unit 6.34 Flow Cytometry–Based Cytotoxicity and Antibody Binding Assay
         Abstract | Full Text:  HTML   PDF

Unit 9.42 Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.31 A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non-Vesicular Organelles
         Abstract | Full Text:  HTML   PDF

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  Supplement 65, July 2013

Unit 1.2 Fluidics
         Abstract | Full Text:  HTML   PDF

Unit 2.21 Building a Live Cell Microscope: What You Need and How to Do It
         Abstract | Full Text:  HTML   PDF

Unit 9.41 Kinetic Viability Assays Using DRAQ7 Probe
         Abstract | Full Text:  HTML   PDF

Unit 11.20 Cytometry in Malaria—A Practical Replacement for Microscopy?
         Abstract | Full Text:  HTML   PDF

Unit 12.30 A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures
         Abstract | Full Text:  HTML   PDF

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  Supplement 64, April 2013

Unit 9.2 Assessment of Cell Viability
         Abstract | Full Text:  HTML   PDF

Unit 9.11 Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes
         Abstract | Full Text:  HTML   PDF

Unit 9.23 Flow Cytometry of the Side Population (SP)
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Time-Lapse Microscopy Approaches to Track Cell Cycle and Lineage Progression at the Single-Cell Level
         Abstract | Full Text:  HTML   PDF

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  Supplement 63, January 2013

Unit 1.27 Spectral Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 1.28 Evaluation and Purchase of an Analytical Flow Cytometer: Some of the Numerous Factors to Consider
         Abstract | Full Text:  HTML   PDF

Unit 2.1 Contrast Enhancement in Light Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 2.20 Comparative and Practical Aspects of Localization-Based Super-Resolution Imaging
         Abstract | Full Text:  HTML   PDF

Unit 9.40 Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 62, October 2012

Unit 2.17 Super-Resolution Microscopy: A Comparative Treatment
         Abstract | Full Text:  HTML   PDF

Unit 2.18 Quantitative Fluorescent Speckle Microscopy (QFSM) to Measure Actin Dynamics
         Abstract | Full Text:  HTML   PDF

Unit 2.19 Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching (FRAP)
         Abstract | Full Text:  HTML   PDF

Unit 12.9 Live-Animal Imaging of Renal Function by Multiphoton Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 61, July 2012

Unit 6.33 Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues
         Abstract | Full Text:  HTML   PDF

Unit 9.39 Ex Vivo Imaging of Excised Tissue Using Vital Dyes and Confocal Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 10.18 Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org)
         Abstract | Full Text:  HTML   PDF

Unit 12.29 Total Internal Reflection Fluorescence (TIRF) Microscopy Illuminator for Improved Imaging of Cell Surface Events
         Abstract | Full Text:  HTML   PDF

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  Supplement 60, April 2012

Unit 1.26 Fountain Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.26 Two-Photon Imaging of the Immune System
         Abstract | Full Text:  HTML   PDF

Unit 12.27 Near-Infrared Molecular Probes for In Vivo Imaging
         Abstract | Full Text:  HTML   PDF

Unit 12.28 Live Imaging of the Lung
         Abstract | Full Text:  HTML   PDF

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  Supplement 59, January 2012

Unit 1.25 Capture of Fluorescence Decay Times by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 9.38 A Violet Ratiometric Membrane Probe for the Detection of Apoptosis
         Abstract | Full Text:  HTML   PDF

Unit 12.24 Practical Methods for Molecular In Vivo Optical Imaging
         Abstract | Full Text:  HTML   PDF

Unit 12.25 Characterization of Surface FAS—Quantitative Morphological Analysis Using Quantitative Imaging Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 58, October 2011

Unit 2.2 Microscope Objectives
         Abstract | Full Text:  HTML   PDF

Unit 2.3 Light Microscopy Digital Imaging
         Abstract | Full Text:  HTML   PDF

Unit 9.37 Assessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress
         Abstract | Full Text:  HTML   PDF

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  Supplement 57, July 2011

Unit 7.40 Zinc Fixation for Flow Cytometry Analysis of Intracellular and Surface Epitopes, DNA Content, and Cell Proliferation
         Abstract | Full Text:  HTML   PDF

Unit 12.22 Setting Up and Running an Advanced Light Microscopy and Imaging Facility
         Abstract | Full Text:  HTML   PDF

Unit 12.23 Photoactivation and Imaging of Optical Highlighter Fluorescent Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 56, April 2011

Unit 2.4 Optical Filters for Wavelength Selection in Fluorescence Instrumentation
         Abstract | Full Text:  HTML   PDF

Unit 6.32 Quantitative Assessment of Pancreatic Islets Using Laser Scanning Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.2 Critical Aspects in Analysis of Cellular DNA Content
         Abstract | Full Text:  HTML   PDF

Unit 7.39 High-Resolution Cell Cycle and DNA Ploidy Analysis in Tissue Samples
         Abstract | Full Text:  HTML   PDF

Unit 9.36 In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 55, January 2011

Unit 6.27 Assessment of Beta Cell Viability
         Abstract | Full Text:  HTML   PDF

Unit 6.31 Fluorescent Cell Barcoding for Multiplex Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.37 High-Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin-Embedded Tissues
         Abstract | Full Text:  HTML   PDF

Unit 7.38 Dual-Pulse Labeling Using 5-Ethynyl-2′-Deoxyuridine (EdU) and 5-Bromo-2′-Deoxyuridine (BrdU) in Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 54, October 2010

Unit 2.9 Multi-Photon Imaging
         Abstract | Full Text:  HTML   PDF

Unit 2.16 Evaluation and Purchase of Confocal Microscopes: Numerous Factors to Consider
         Abstract | Full Text:  HTML   PDF

Unit 6.29 Titration of Fluorochrome-Conjugated Antibodies for Labeling Cell Surface Markers on Live Cells
         Abstract | Full Text:  HTML   PDF

Unit 6.30 Phenotypic Analysis Using Very Small Volumes of Blood
         Abstract | Full Text:  HTML   PDF

Unit 12.21 From Image to Data Using Common Image-Processing Techniques
         Abstract | Full Text:  HTML   PDF

Unit 13.12 Microsphere-Based Flow Cytometry Protease Assays for Use in Protease Activity Detection and High-Throughput Screening
         Abstract | Full Text:  HTML   PDF

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  Supplement 53, July 2010

Unit 9.34 Amine-Reactive Dyes for Dead Cell Discrimination in Fixed Samples
         Abstract | Full Text:  HTML   PDF

Unit 9.35 Detection of Intracellular Glutathione Using ThiolTracker Violet Stain and Fluorescence Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 10.17 Web-Based Analysis and Publication of Flow Cytometry Experiments
         Abstract | Full Text:  HTML   PDF

Unit 12.20 Approaches to Spectral Imaging Hardware
         Abstract | Full Text:  HTML   PDF

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  Supplement 52, April 2010

Unit 7.2 Critical Aspects in Analysis of Cellular DNA Content
         Abstract | Full Text:  HTML   PDF

Unit 7.36 Click-iT Proliferation Assay with Improved DNA Histograms
         Abstract | Full Text:  HTML   PDF

Unit 9.32 Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology
         Abstract | Full Text:  HTML   PDF

Unit 9.33 Identification of Endothelial Cells and Progenitor Cell Subsets in Human Peripheral Blood
         Abstract | Full Text:  HTML   PDF

Unit 11.19 Life Cycle Analysis of Unicellular Algae
         Abstract | Full Text:  HTML   PDF

Unit 12.19 3D Deconvolution Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 51, January 2010

Unit 1.24 Practical Issues in High-Speed Cell Sorting
         Abstract | Full Text:  HTML   PDF

Unit 6.28 Measurement of T Cell Activation After 16-hr In Vitro Stimulation with Concanavalin A
         Abstract | Full Text:  HTML   PDF

Unit 9.29 Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods
         Abstract | Full Text:  HTML   PDF

Unit 9.30 Stem Cell Side Population Analysis and Sorting Using DyeCycle Violet
         Abstract | Full Text:  HTML   PDF

Unit 9.31 Measurement of Phagocytosis and of the Phagosomal Environment in Polymorphonuclear Phagocytes by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 13.11 Use of Flow Cytometric Methods to Quantify Protein-Protein Interactions
         Abstract | Full Text:  HTML   PDF

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  Supplement 50, October 2009

Unit 1.10 Techniques for Flow Cytometer Alignment
         Abstract | Full Text:  HTML   PDF

Unit 1.23 Pulse Width for Particle Sizing
         Abstract | Full Text:  HTML   PDF

Unit 2.15 Measurement of Molecular Mobility with Fluorescence Correlation Spectroscopy
         Abstract | Full Text:  HTML   PDF

Unit 6.27 High-Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin-Embedded Tissues
         Abstract | Full Text:  HTML   PDF

Unit 12.18 Total Internal Reflection Fluorescence (TIRF) Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 49, July 2009

Unit 1.9 Lasers for Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 1.22 Fundamentals of Acoustic Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.26 Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements
         Abstract | Full Text:  HTML   PDF

Unit 11.18 Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single-Cell Level by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.17 Simultaneous Optical Mapping of Intracellular Free Calcium and Action Potentials from Langendorff Perfused Hearts
         Abstract | Full Text:  HTML   PDF

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  Supplement 48, April 2009

Unit 12.15 Cryosectioning
         Abstract | Full Text:  HTML   PDF

Unit 12.16 Immunohistochemistry
         Abstract | Full Text:  HTML   PDF

Unit 13.10 Multiplexed Analysis of Peptide Antigen-Specific Antibodies
         Abstract | Full Text:  HTML   PDF

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  Supplement 47, January 2009

Unit 1.4 Establishing and Maintaining System Linearity
         Abstract | Full Text:  HTML   PDF

Unit 9.28 Measurement of Cytoplasmic to Nuclear Translocation
         Abstract | Full Text:  HTML   PDF

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  Supplement 46, October 2008

Unit 2.3 Light Microscopy Digital Imaging
         Abstract | Full Text:  HTML   PDF

Unit 7.35 Assessment of Histone Acetylation Levels in Relation to Cell Cycle Phase
         Abstract | Full Text:  HTML   PDF

Unit 9.26 Immunophenotyping and DNA Content Analysis of Acetone-Fixed Cells
         Abstract | Full Text:  HTML   PDF

Unit 9.27 Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 45, July 2008

Unit 4.7 Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries
         Abstract | Full Text:  HTML   PDF

Unit 6.25 Flow Cytometric Immunophenotyping of Cerebrospinal Fluid
         Abstract | Full Text:  HTML   PDF

Unit 7.34 Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining
         Abstract | Full Text:  HTML   PDF

Unit 12.13 Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide-Based Signaling Events in Isolated Perfused Lung
         Abstract | Full Text:  HTML   PDF

Unit 12.14 Flow Cytometric FRET Analysis of ErbB Receptor Tyrosine Kinase Interaction
         Abstract | Full Text:  HTML   PDF

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  Supplement 44, April 2008

Unit 9.25 Advanced Application of CFSE for Cellular Tracking
         Abstract | Full Text:  HTML   PDF

Unit 12.12 From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism
         Abstract | Full Text:  HTML   PDF

Unit 13.9 Multiplexed Detection of Fungal Nucleic Acid Signatures
         Abstract | Full Text:  HTML   PDF

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  Supplement 43, January 2008

Unit 2.14 Methods and Applications of Laser-Enabled Analysis and Processing (LEAP)
         Abstract | Full Text:  HTML   PDF

Unit 12.11 Confocal Imaging of Cell Division
         Abstract | Full Text:  HTML   PDF

Appendix 3C Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
         Abstract | Full Text:  HTML   PDF

Appendix 3I Production of Polyclonal Antisera
         Abstract | Full Text:  HTML   PDF

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  Supplement 42, October 2007

Unit 4.6 Flow Cytometric Sorting of Bacterial Surface-Displayed Libraries
         Abstract | Full Text:  HTML   PDF

Unit 7.33 SYTO Probes: Markers of Apoptotic Cell Demise
         Abstract | Full Text:  HTML   PDF

Unit 10.16 Alternatives to Log-Scale Data Display
         Abstract | Full Text:  HTML   PDF

Unit 12.10 Detecting Protein-Protein Interactions In Vivo with FRET using Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM)
         Abstract | Full Text:  HTML   PDF

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  Supplement 41, July 2007

Unit 7.32 Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1
         Abstract | Full Text:  HTML   PDF

Unit 9.24 High-Throughput Cytotoxicity Screening by Propidium Iodide Staining
         Abstract | Full Text:  HTML   PDF

Unit 12.9 Live-Animal Imaging of Renal Function by Multiphoton Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 40, April 2007

Unit 1.20 Characterization of Flow Cytometer Instrument Sensitivity
         Abstract | Full Text:  HTML   PDF

Unit 1.21 Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer
         Abstract | Full Text:  HTML   PDF

Unit 7.31 Assessment of Cell Proliferation by 5-Bromodeoxyuridine (BrdU) Labeling for Multicolor Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 39, January 2007

Unit 1.1 Overview of Flow Cytometry Instrumentation
         Abstract | Full Text:  HTML   PDF

Unit 3.6 Standard Safety Practices for Sorting of Unfixed Cells
         Abstract | Full Text:  HTML   PDF

Unit 9.22 Optimized Whole-Blood Assay for Measurement of ZAP-70 Protein Expression
         Abstract | Full Text:  HTML   PDF

Unit 9.23 Flow Cytometry of the Side Population (SP)
         Abstract | Full Text:  HTML   PDF

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  Supplement 38, October 2006

Unit 1.12 Principles of Resonance Energy Transfer
         Abstract | Full Text:  HTML   PDF

Unit 2.13 Optimizing Laser Source Operation for Confocal and Multiphoton Laser Scanning Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 7.30 Estimation of Relative Nuclear DNA Content in Dehydrated Plant Tissues by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.8 Measuring FRET in Flow Cytometry and Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 37, July 2006

Unit 10.14 Intensity Calibration and Shading Correction for Fluorescence Microscopes
         Abstract | Full Text:  HTML   PDF

Unit 13.8 Reagents and Instruments for Multiplexed Analysis Using Microparticles
         Abstract | Full Text:  HTML   PDF

Appendix 3I Production of Polyclonal Antisera
          HTML   PDF

Appendix 3J Production of Monoclonal Antibodies
         Abstract | Full Text:  HTML   PDF

Appendix 3K Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
         Abstract | Full Text:  HTML   PDF

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  Supplement 36, April 2006

Unit 3.4 Safe Use of Hazardous Chemicals
         Abstract | Full Text:  HTML   PDF

Unit 6.23 Immunophenotyping of Plasma Cells
         Abstract | Full Text:  HTML   PDF

Unit 6.24 Flow Rate Calibration for Absolute Cell Counting Rationale and Design
         Abstract | Full Text:  HTML   PDF

Appendix 3B Techniques for Mammalian Cell Tissue Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 35, January 2006

Unit 7.29 Nuclear DNA Content Analysis of Plant Seeds by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.7 Detecting Protein–Protein Interactions with CFP-YFP FRET by Acceptor Photobleaching
         Abstract | Full Text:  HTML   PDF

Unit 13.1 Multiplexed Microsphere-Based Flow Cytometric Immunoassays
         Abstract | Full Text:  HTML   PDF

Unit 13.6 Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
         Abstract | Full Text:  HTML   PDF

Unit 13.7 Detection of Gene Fusions in Acute Leukemia Using Bead Microarrays
         Abstract | Full Text:  HTML   PDF

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  Supplement 34, October 2005

Unit 13.2 Microsphere Surface Protein Determination Using Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 13.3 Use of Microsphere-Supported Phospholipid Membranes for Analysis of Protein-Lipid Interactions
         Abstract | Full Text:  HTML   PDF

Unit 13.4 Multiplexed SNP Genotyping Using Primer Single-Base Extension (SBE) and Microsphere Arrays
         Abstract | Full Text:  HTML   PDF

Unit 13.5 BeadCons: Detection of Nucleic Acid Sequences by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 33, July 2005

Unit 6.21 Ten-Color Immunophenotyping of Hematopoietic Cells
         Abstract | Full Text:  HTML   PDF

Unit 6.22 Flow Cytometric Screening for the HLA-B27 Antigen on Peripheral Blood Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 7.28 RNA and DNA Aptamers in Cytomics Analysis
         Abstract | Full Text:  HTML   PDF

Unit 12.6 Quantitative Fluorescence In Situ Hybridization (QFISH) of Telomere Lengths in Tissue and Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 32, April 2005

Unit 1.3 Standardization, Calibration, and Control in Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.19 ZAP-70 Staining in Chronic Lymphocytic Leukemia
         Abstract | Full Text:  HTML   PDF

Unit 6.20 Multiparameter Analysis of Intracellular Phosphoepitopes in Immunophenotyped Cell Populations by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.5 Three-Dimensional Visualization of Blood and Lymphatic Vasculature in Tissue Whole Mounts Using Confocal Microscopy
         Abstract | Full Text:  HTML   PDF

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  Supplement 31, January 2005

Unit 12.1 Comparative Overview of Flow and Image Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 12.2 Basics of Digital Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 12.3 Modern Confocal Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 12.4 Time-Lapse Microscopy Approaches to Track Cell Cycle Progression at the Single-Cell Level
         Abstract | Full Text:  HTML   PDF

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  Supplement 30, October 2004

Unit 6.3 High-Sensitivity Immunofluorescence/Flow Cytometry: Detection of Cytokine Receptors and Other Low-Abundance Membrane Molecules
         Abstract | Full Text:  HTML   PDF

Unit 6.18 Identification of Human Antigen-Specific T Cells Using MHC Class I and Class II Tetramers
         Abstract | Full Text:  HTML   PDF

Unit 7.3 Differential Staining of DNA and RNA
         Abstract | Full Text:  HTML   PDF

Unit 7.27 Detection of Histone H2AX Phosphorylation on Ser-139 as an Indicator of DNA Damage (DNA Double-Strand Breaks)
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Overview of Flow Cytometry and Microbiology
         Abstract | Full Text:  HTML   PDF

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  Supplement 29, July 2004

Unit 7.26 Assessment of Telomere Length, Phenotype, and DNA Content
         Abstract | Full Text:  HTML   PDF

Unit 11.3 Estimation of Microbial Viability Using Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 11.17 Labeling of Bacterial Pathogens for Flow Cytometric Detection and Enumeration
         Abstract | Full Text:  HTML   PDF

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  Supplement 28, April 2004

Unit 2.10 Scanning Laser Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.17 Enumeration of Fetal Red Blood Cells, F Cells, and F Reticulocytes in Human Blood
         Abstract | Full Text:  HTML   PDF

Unit 7.24 Detection of Mitotic Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.25 DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells
         Abstract | Full Text:  HTML   PDF

Unit 9.6 Estimation of Membrane Potential by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 9.9 Intracellular Cytokines
         Abstract | Full Text:  HTML   PDF

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  Supplement 27, January 2004

Unit 1.9 Lasers for Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.16 Flow Cytometric Assessment of HLA Alloantibodies
         Abstract | Full Text:  HTML   PDF

Unit 8.11 Principles and Applications of PRINS in Cytogenetics
         Abstract | Full Text:  HTML   PDF

Unit 9.11 Flow Cytometric Analysis of Cell Division by Dye Dilution
         Abstract | Full Text:  HTML   PDF

Unit 11.2 Flow Cytometry and Environmental Microbiology
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Mycobacterium tuberculosis Susceptibility Testing by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 26, October 2003

Unit 1.19 Excitation and Emission Spectra of Common Dyes
         Abstract | Full Text:  HTML   PDF

Unit 4.3 Nucleic Acid Probes
         Abstract | Full Text:  HTML   PDF

Unit 7.22 Analysis of Tissue Imprints by Scanning Laser Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 7.23 Cell Cycle Analysis of Budding Yeast Using SYTOX Green
         Abstract | Full Text:  HTML   PDF

Unit 8.13 Combined Immunofluorescence and FISH: New Prospects for Tumor Cell Detection/Identification
         Abstract | Full Text:  HTML   PDF

Unit 11.5 Detection of Borreliacidal Antibodies by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 25, July 2003

Unit 6.4 Enumeration of CD34 Hematopoietic Stem and Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.19 Flow Cytometry of Apoptosis
         Abstract | Full Text:  HTML   PDF

Unit 9.21 Flow Cytometric Analysis of Cytokine Responses in Stimulated Whole Blood: Simultaneous Quantitation of TNF-α-Secreting Cells and Soluble Cytokines
         Abstract | Full Text:  HTML   PDF

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  Supplement 24, April 2003

Unit 6.15 Whole Blood Analysis of Leukocyte-Platelet Aggregates
         Abstract | Full Text:  HTML   PDF

Unit 9.20 Flow Cytometric Analysis of Calcium Mobilization in Whole-Blood Platelets
         Abstract | Full Text:  HTML   PDF

Unit 11.16 Functional Assays of Oxidative Stress Using Genetically Engineered Escherichia coli Strains
         Abstract | Full Text:  HTML   PDF

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  Supplement 23, January 2003

Unit 6.13 Immunophenotyping Using a Laser Scanning Cytometer
         Abstract | Full Text:  HTML   PDF

Unit 6.14 Enzymatic Amplification Staining for Cell Surface Antigens
         Abstract | Full Text:  HTML   PDF

Unit 7.21 Measurement of Cytogenetic Damage in Rodent Blood with a Single-Laser Flow Cytometer
         Abstract | Full Text:  HTML   PDF

Unit 10.15 A Software Method for Color Compensation
         Abstract | Full Text:  HTML   PDF

Unit 11.15 Resolution of Viable and Membrane-Compromised Free Bacteria in Aquatic Environments by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 22, October 2002

Unit 1.14 Compensation in Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.12 Quantitative Flow Cytometric Analysis of Membrane Antigen Expression
         Abstract | Full Text:  HTML   PDF

Unit 7.19 Flow Cytometry of Apoptosis

Unit 7.20 Analysis of Fine-Needle Aspirate Biopsies from Solid Tumors by Laser Scanning Cytometry (LSC)
         Abstract | Full Text:  HTML   PDF

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  Supplement 21, July 2002

Unit 7.18 Apoptosis Signaling Pathways
         Abstract | Full Text:  HTML   PDF

Unit 9.19 Assessment of Phagocyte Functions by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 10.14 Intensity Calibration and Shading Correction for Fluorescence Microscopes
          HTML   PDF

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  Supplement 20, April 2002

Unit 1.18 Dynamic Thermoregulation of the Sample in Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 3.4 Safe Use of Hazardous Chemicals
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Unit 6.11 Immunophenotypic Analysis of PNH Cells
         Abstract | Full Text:  HTML   PDF

Unit 10.13 The Application of Data Mining to Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Appendix 1A Abbreviations Used in this Manual
         Abstract | Full Text:  HTML   PDF

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  Supplement 19, January 2002

Unit 3.5 Method for Visualizing Aerosol Contamination in Flow Sorters
         Abstract | Full Text:  HTML   PDF

Unit 6.10 Immunophenotypic Analysis of Platelets
         Abstract | Full Text:  HTML   PDF

Unit 9.18 Stem Cell Identification and Sorting Using the Hoechst 33342 Side Population (SP)
         Abstract | Full Text:  HTML   PDF

Unit 10.12 Dial-In Flow Cytometry Data Analysis
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, October 2001

Unit 3.4 Safe Use of Hazardous Chemicals

Unit 8.11 Principles and Applications of PRINS in Cytogenetics
         Abstract | Full Text:  HTML   PDF

Unit 8.12 Comparative Genomic Hybridization (CGH)—Detection of Unbalanced Genetic Aberrations Using Conventional and Micro-Array Techniques
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, July 2001

Unit 1.17 Plug Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.9 Immunophenotypic Identification, Enumeration, and Characterization of Human Peripheral Blood Dendritic Cells and Dendritic-Cell Precursors
         Abstract | Full Text:  HTML   PDF

Unit 7.17 Analysis of Viral Infection and Viral and Cellular DNA and Proteins by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 9.17 Assessment of Surface Markers and Functionality of Dendritic Cells (DCs)
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, April 2001

Unit 1.16 Simultaneous Analysis of the Cyan, Green, and Yellow Fluorescent Proteins
         Abstract | Full Text:  HTML   PDF

Unit 7.15 Ultraviolet-Induced Detection of Halogenated Pyrimidines (UVID)
         Abstract | Full Text:  HTML   PDF

Unit 7.16 Analysis of DNA Content and Green Fluorescent Protein Expression
         Abstract | Full Text:  HTML   PDF

Unit 8.8 Single-Nucleotide Sequence Discrimination In Situ Using Padlock Probes
         Abstract | Full Text:  HTML   PDF

Unit 8.10 Molecular Combing
         Abstract | Full Text:  HTML   PDF

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  Supplement 15, January 2001

Unit 2.4 Optical Filtering Systems for Wavelength Selection in Light Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 2.12 Photobleaching Measurements of Diffusion in Cell Membranes and Aqueous Cell Compartments
         Abstract | Full Text:  HTML   PDF

Unit 7.14 Analysis of Cell Proliferation and Cell Survival by Continuous BrdU Labeling and Multivariate Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 8.11 Principles and Applications of PRINS in Cytogenetics

Unit 11.14 Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes
         Abstract | Full Text:  HTML   PDF

Appendix 1B Common Conversion Factors
         Abstract | Full Text:  HTML   PDF

Appendix 3H Importing Biological Materials
         Abstract | Full Text:  HTML   PDF

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  Supplement 14, October 2000

Unit 2.11 Shading Correction: Compensation for Illumination and Sensor Inhomogeneities
         Abstract | Full Text:  HTML   PDF

Unit 4.5 Spectroscopic Analysis Using DNA and RNA Fluorescent Probes
         Abstract | Full Text:  HTML   PDF

Unit 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 9.15 Multiparameter Analysis of Physiological Changes in Apoptosis
         Abstract | Full Text:  HTML   PDF

Unit 9.16 Signal Transduction During Natural Killer Cell Activation
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, July 2000

Unit 6.8 Enumeration of Absolute Cell Counts Using Immunophenotypic Techniques
         Abstract | Full Text:  HTML   PDF

Unit 7.13 Sperm Chromatin Structure Assay for Fertility Assessment
         Abstract | Full Text:  HTML   PDF

Unit 9.14 Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1
         Abstract | Full Text:  HTML   PDF

Unit 10.11 Image Processing and 2-D Morphometry
         Abstract | Full Text:  HTML   PDF

Unit 11.13 Cell Cycle Analysis of Yeasts
         Abstract | Full Text:  HTML   PDF

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  Supplement 12, April 2000

Unit 6.6 Immunophenotypic Analysis of Human Mast Cells by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.7 Measurement of CD40 Ligand (CD154) Expression on Resting and In Vitro–Activated T Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.12 Flow Cytometric Analysis of RNA Synthesis by Detection of Bromouridine Incorporation
         Abstract | Full Text:  HTML   PDF

Unit 9.13 In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix
         Abstract | Full Text:  HTML   PDF

Appendix 3G Growing Bacteria on Solid Media
         Abstract | Full Text:  HTML   PDF

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  Supplement 11, January 2000

Unit 1.15 Time-Resolved Fluorescence Measurements
         Abstract | Full Text:  HTML   PDF

Unit 6.5 Immunophenotypic Analysis of Peripheral Blood Lymphocytes
         Abstract | Full Text:  HTML   PDF

Unit 8.9 Tyramide Signal Amplification (TSA) Systems for the Enhancement of ISH Signals in Cytogenetics
         Abstract | Full Text:  HTML   PDF

Unit 11.12 DNA/RNA Analysis of Phytoplankton by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Appendix 3C Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
          HTML   PDF

Appendix 3D Wright-Giemsa and Nonspecific Esterase Staining of Cells
         Abstract | Full Text:  HTML   PDF

Appendix 3E Techniques for Bacterial Cell Culture: Media Preparation and Bacteriological Tools
         Abstract | Full Text:  HTML   PDF

Appendix 3F Growing Bacteria in Liquid Media
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, October 1999

Unit 1.14 Compensation in Flow Cytometry

Unit 2.10 Scanning Laser Cytometry

Unit 7.11 Assessment of Viability, Immunofluorescence, and DNA Content
         Abstract | Full Text:  HTML   PDF

Unit 10.10 Three-Dimensional Image Visualization and Analysis
         Abstract | Full Text:  HTML   PDF

Unit 11.11 Enumeration of Phytoplankton, Bacteria, and Viruses in Marine Samples
         Abstract | Full Text:  HTML   PDF

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  Supplement 9, July 1999

Unit 1.12 Principles of Resonance Energy Transfer
          HTML   PDF

Unit 1.13 Light Scatter: Detection and Usage
         Abstract | Full Text:  HTML   PDF

Unit 5.3 Flow Analysis and Sorting of Plant Chromosomes
         Abstract | Full Text:  HTML   PDF

Unit 8.8 Single-Nucleotide Sequence Discrimination In Situ Using Padlock Probes

Unit 11.9 Determination of Bacterial Biomass from Flow Cytometric Measurements of Forward Light Scatter Intensity
         Abstract | Full Text:  HTML   PDF

Unit 11.10 Flow Cytometry of Yeasts
         Abstract | Full Text:  HTML   PDF

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  Supplement 8, April 1999

Unit 6.4 Enumeration of CD34 Hematopoietic Stem and Progenitor Cells

Unit 11.6 Flow Cytometric Detection of Pathogenic E. coli in Food
         Abstract | Full Text:  HTML   PDF

Unit 11.7 Mycobacterium tuberculosis Susceptibility Testing by Flow Cytometry

Unit 11.8 Antibiotic Susceptibility Testing by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 7, January 1999

Unit 1.11 Flow Cytometers for Characterization of Microorganisms
         Abstract | Full Text:  HTML   PDF

Unit 9.12 Reporters of Gene Expression: Autofluorescent Proteins
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Overview of Flow Cytometry and Microbiology
          HTML   PDF

Unit 11.2 Flow Cytometry and Environmental Microbiology

Unit 11.3 Estimation of Microbial Viability Using Flow Cytometry
          HTML   PDF

Unit 11.4 Sorting of Bacteria
         Abstract | Full Text:  HTML   PDF

Unit 11.5 Detection of Borreliacidal Antibodies by Flow Cytometry

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  Supplement 6, October 1998

Unit 1.10 Techniques for Flow Cytometer Alignment
         Full Text:  HTML   PDF

Unit 2.9 Multiphoton Imaging
         Abstract | Full Text:  HTML   PDF

Unit 7.10 Flow Cytometric Analysis of Reticulated Platelets
         Abstract | Full Text:  HTML   PDF

Unit 8.7 Combination DNA/RNA Fish and Immunophenotyping
         Abstract | Full Text:  HTML   PDF

Unit 9.5 Reporters of Gene Expression: Enzymatic Assays
         Abstract | Full Text:  HTML   PDF

Unit 9.11 Flow Cytometric Analysis of Cell Division by Dye Dilution

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  Supplement 5, July 1998

Unit 2.3 Cameras
         Abstract | Full Text:  HTML   PDF

Unit 2.8 Confocal Microscopy: Principles and Practices
         Abstract | Full Text:  HTML   PDF

Unit 6.3 High-Sensitivity Immunofluorescence/Flow Cytometry: Detection of Cytokine Receptors and Other Low-Abundance Membrane Molecules
          HTML   PDF

Unit 8.5 Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics
         Abstract | Full Text:  HTML   PDF

Unit 8.6 Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, April 1998

Unit 1.9 Lasers for Flow Cytometry

Unit 2.6 Calibration: Sampling Density and Spatial Resolution
         Abstract | Full Text:  HTML   PDF

Unit 7.9 Bivariate Analysis of DNA Content and Expression of Cyclin Proteins
         Abstract | Full Text:  HTML   PDF

Unit 8.4 Immunocytochemical Detection
         Abstract | Full Text:  HTML   PDF

Unit 9.10 Assays of Natural Killer (NK) Cell Ligation to Target Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, January 1998

Unit 1.8 Principles of Gating
         Abstract | Full Text:  HTML   PDF

Unit 7.8 Analysis of DNA Denaturation
         Abstract | Full Text:  HTML   PDF

Unit 9.9 Intracellular Cytokines

Unit 10.8 Multivariate Analysis
         Abstract | Full Text:  HTML   PDF

Unit 10.9 Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, October 1997

Unit 2.7 Microscope Alignment
         Abstract | Full Text:  HTML   PDF

Unit 4.4 Cellular Function Probes
         Abstract | Full Text:  HTML   PDF

Unit 7.6 Analysis of Nuclear DNA Content and Ploidy in Higher Plants
         Abstract | Full Text:  HTML   PDF

Unit 7.7 Analysis of DNA Content and BrdU Incorporation
         Abstract | Full Text:  HTML   PDF

Unit 9.7 Oxidative Metabolism
         Abstract | Full Text:  HTML   PDF

Unit 9.8 Measurement of Intracellular Calcium Ions by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

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  Supplement 1, July 1997

Unit 1.2 Fluidics
         Abstract | Full Text:  HTML   PDF

Unit 1.6 Laser Beam Shaping and Spot Size
         Abstract | Full Text:  HTML   PDF

Unit 1.7 High-Speed Cell Sorting
         Abstract | Full Text:  HTML   PDF

Unit 2.6 Calibration: Sampling Density and Spatial Resolution

Unit 3.3 Testing the Efficiency of Aerosol Containment During Cell Sorting
         Abstract | Full Text:  HTML   PDF

Unit 9.6 Estimation of Membrane Potential by Flow Cytometry

Unit 10.6 Data Presentation
         Abstract | Full Text:  HTML   PDF

Unit 10.7 Data Analysis Through Modeling
         Abstract | Full Text:  HTML   PDF

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  Core Publication, April 1997

Unit 1.1 Overview of Flow Cytometry Instrumentation
          HTML   PDF

Unit 1.3 Standardization, Calibration, and Control in Flow Cytometry
          HTML   PDF

Unit 1.4 Establishing and Maintaining System Linearity
         HTML   PDF

Unit 1.5 Optical Filter Sets for Multiparameter Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 2.1 Contrast Enhancement in Light Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 2.2 Microscope Objectives
         Abstract | Full Text:  HTML   PDF

Unit 2.3 Cameras

Unit 2.4 Optical Filtering Systems for Wavelength Selection in Light Microscopy

Unit 2.5 Digital Fluorescence Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 3.1 Principles of Quality Control
         Abstract | Full Text:  HTML   PDF

Unit 3.2 Components of Quality Control
         Abstract | Full Text:  HTML   PDF

Unit 4.1 Titering Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 4.2 Conjugation of Fluorochromes to Monoclonal Antibodies
         Abstract | Full Text:  HTML   PDF

Unit 4.3 Nucleic Acid Probes

Unit 5.1 Handling, Storage, and Preparation of Human Blood Cells
         Abstract | Full Text:  HTML   PDF

Unit 5.2 Handling, Storage, and Preparation of Human Tissues
         Abstract | Full Text:  HTML   PDF

Unit 6.1 Quality Control in Phenotypic Analysis by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 6.2 Immunophenotyping
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Overview of Nucleic Acid Analysis
         Abstract | Full Text:  HTML   PDF

Unit 7.2 Quality Control in Nucleic Acid Analysis
         Full Text:  HTML   PDF

Unit 7.3 Differential Staining of DNA and RNA
         Full Text:  HTML   PDF

Unit 7.4 Analysis of DNA Content and DNA Strand Breaks for Detection of Apoptotic Cells
         Abstract | Full Text:  HTML   PDF

Unit 7.5 DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis
         Abstract | Full Text:  HTML   PDF

Unit 8.1 Overview of Fluorescence In Situ Hybridization Techniques for Molecular Cytogenetics
         Abstract | Full Text:  HTML   PDF

Unit 8.2 Basic Preparative Techniques for Fluorescence In Situ Hybridization
         Abstract | Full Text:  HTML   PDF

Unit 8.3 Probe Labeling and Fluorescence In Situ Hybridization
         Abstract | Full Text:  HTML   PDF

Unit 9.1 Overview of Functional Cell Assays
         Abstract | Full Text:  HTML   PDF

Unit 9.2 Assessment of Cell Viability
         Abstract | Full Text:  HTML   PDF

Unit 9.3 Flow Cytometric Measurement of Intracellular pH
         Abstract | Full Text:  HTML   PDF

Unit 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy

Unit 9.5 Reporters of Gene Expression: Enzymatic Assays

Unit 10.1 Data Management
         Abstract | Full Text:  HTML   PDF

Unit 10.2 Data File Standard for Flow Cytometry, FCS 3.0
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Listmode Data Processing
         Abstract | Full Text:  HTML   PDF

Unit 10.4 Multidimensional Data Analysis in Immunophenotyping
         Abstract | Full Text:  HTML   PDF

Unit 10.5 Two-Dimensional Image Processing and Analysis
         Abstract | Full Text:  HTML   PDF

Appendix 1A Abbreviations Used in this Manual

Appendix 1B Common Conversion Factors

Appendix 2A Common Stock Solutions, Buffers, and Media
         Abstract | Full Text:  HTML   PDF

Appendix 3A Cell Counting
         Abstract | Full Text:  HTML   PDF

Appendix 3B Techniques for Mammalian Cell Tissue Culture
         Full Text:  HTML   PDF

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