Current Protocols in Cytometry
Online ISBN: 9780471142959
DOI: 10.1002/0471142956
Archive
2013: Supplement 64 Supplement 63
2012: Supplement 62 Supplement 61 Supplement 60 Supplement 59
2011: Supplement 58 Supplement 57 Supplement 56 Supplement 55
2010: Supplement 54 Supplement 53 Supplement 52 Supplement 51
2009: Supplement 50 Supplement 49 Supplement 48 Supplement 47
2008: Supplement 46 Supplement 45 Supplement 44 Supplement 43
2007: Supplement 42 Supplement 41 Supplement 40 Supplement 39
2006: Supplement 38 Supplement 37 Supplement 36 Supplement 35
2005: Supplement 34 Supplement 33 Supplement 32 Supplement 31
2004: Supplement 30 Supplement 29 Supplement 28 Supplement 27
2003: Supplement 26 Supplement 25 Supplement 24 Supplement 23
2002: Supplement 22 Supplement 21 Supplement 20 Supplement 19
2001: Supplement 18 Supplement 17 Supplement 16 Supplement 15
2000: Supplement 16 Supplement 13 Supplement 12 Supplement 11
1999: Supplement 10 Supplement 9 Supplement 8 Supplement 7
1998: Supplement 6 Supplement 5 Supplement 4 Supplement 3
1997: Supplement 2 Supplement 1
1998: Core publication
Supplement 64, April 2013
Unit 9.2 Assessment of Cell Viability
Abstract | Full Text: HTML PDF
Unit 9.11 Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes
Abstract | Full Text: HTML PDF
Unit 9.23 Flow Cytometry of the Side Population (SP)
Abstract | Full Text: HTML PDF
Unit 12.4 Time-Lapse Microscopy Approaches to Track Cell Cycle and Lineage Progression at the Single-Cell Level
Abstract | Full Text: HTML PDF
Supplement 63, January 2013
Unit 1.27 Spectral Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 1.28 Evaluation and Purchase of an Analytical Flow Cytometer: Some of the Numerous Factors to Consider
Abstract | Full Text: HTML PDF
Unit 2.1 Contrast Enhancement in Light Microscopy
Abstract | Full Text: HTML PDF
Unit 2.20 Comparative and Practical Aspects of Localization-Based Super-Resolution Imaging
Abstract | Full Text: HTML PDF
Unit 9.40 Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture
Abstract | Full Text: HTML PDF
Supplement 62, October 2012
Unit 2.17 Super-Resolution Microscopy: A Comparative Treatment
Abstract | Full Text: HTML PDF
Unit 2.18 Quantitative Fluorescent Speckle Microscopy (QFSM) to Measure Actin Dynamics
Abstract | Full Text: HTML PDF
Unit 2.19 Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching (FRAP)
Abstract | Full Text: HTML PDF
Unit 12.9 Live-Animal Imaging of Renal Function by Multiphoton Microscopy
Abstract | Full Text: HTML PDF
Supplement 61, July 2012
Unit 6.33 Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues
Abstract | Full Text: HTML PDF
Unit 9.39 Ex Vivo Imaging of Excised Tissue Using Vital Dyes and Confocal Microscopy
Abstract | Full Text: HTML PDF
Unit 10.18 Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org)
Abstract | Full Text: HTML PDF
Unit 12.29 Total Internal Reflection Fluorescence (TIRF) Microscopy Illuminator for Improved Imaging of Cell Surface Events
Abstract | Full Text: HTML PDF
Supplement 60, April 2012
Unit 1.26 Fountain Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 12.26 Two-Photon Imaging of the Immune System
Abstract | Full Text: HTML PDF
Unit 12.27 Near-Infrared Molecular Probes for In Vivo Imaging
Abstract | Full Text: HTML PDF
Unit 12.28 Live Imaging of the Lung
Abstract | Full Text: HTML PDF
Supplement 59, January 2012
Unit 1.25 Capture of Fluorescence Decay Times by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 9.38 A Violet Ratiometric Membrane Probe for the Detection of Apoptosis
Abstract | Full Text: HTML PDF
Unit 12.24 Practical Methods for Molecular In Vivo Optical Imaging
Abstract | Full Text: HTML PDF
Unit 12.25 Characterization of Surface FAS—Quantitative Morphological Analysis Using Quantitative Imaging Cytometry
Abstract | Full Text: HTML PDF
Supplement 58, October 2011
Unit 2.2 Microscope Objectives
Abstract | Full Text: HTML PDF
Unit 2.3 Light Microscopy Digital Imaging
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Unit 9.37 Assessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress
Abstract | Full Text: HTML PDF
Supplement 57, July 2011
Unit 7.40 Zinc Fixation for Flow Cytometry Analysis of Intracellular and Surface Epitopes, DNA Content, and Cell Proliferation
Abstract | Full Text: HTML PDF
Unit 12.22 Setting Up and Running an Advanced Light Microscopy and Imaging Facility
Abstract | Full Text: HTML PDF
Unit 12.23 Photoactivation and Imaging of Optical Highlighter Fluorescent Proteins
Abstract | Full Text: HTML PDF
Supplement 56, April 2011
Unit 2.4 Optical Filters for Wavelength Selection in Fluorescence Instrumentation
Abstract | Full Text: HTML PDF
Unit 6.32 Quantitative Assessment of Pancreatic Islets Using Laser Scanning Cytometry
Abstract | Full Text: HTML PDF
Unit 7.2 Critical Aspects in Analysis of Cellular DNA Content
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Unit 7.39 High-Resolution Cell Cycle and DNA Ploidy Analysis in Tissue Samples
Abstract | Full Text: HTML PDF
Unit 9.36 In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 55, January 2011
Unit 6.27 Assessment of Beta Cell Viability
Abstract | Full Text: HTML PDF
Unit 6.31 Fluorescent Cell Barcoding for Multiplex Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 7.37 High-Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin-Embedded Tissues
Abstract | Full Text: HTML PDF
Unit 7.38 Dual-Pulse Labeling Using 5-Ethynyl-2′-Deoxyuridine (EdU) and 5-Bromo-2′-Deoxyuridine (BrdU) in Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 54, October 2010
Unit 2.9 Multi-Photon Imaging
Abstract | Full Text: HTML PDF
Unit 2.16 Evaluation and Purchase of Confocal Microscopes: Numerous Factors to Consider
Abstract | Full Text: HTML PDF
Unit 6.29 Titration of Fluorochrome-Conjugated Antibodies for Labeling Cell Surface Markers on Live Cells
Abstract | Full Text: HTML PDF
Unit 6.30 Phenotypic Analysis Using Very Small Volumes of Blood
Abstract | Full Text: HTML PDF
Unit 12.21 From Image to Data Using Common Image-Processing Techniques
Abstract | Full Text: HTML PDF
Unit 13.12 Microsphere-Based Flow Cytometry Protease Assays for Use in Protease Activity Detection and High-Throughput Screening
Abstract | Full Text: HTML PDF
Supplement 53, July 2010
Unit 9.34 Amine-Reactive Dyes for Dead Cell Discrimination in Fixed Samples
Abstract | Full Text: HTML PDF
Unit 9.35 Detection of Intracellular Glutathione Using ThiolTracker Violet Stain and Fluorescence Microscopy
Abstract | Full Text: HTML PDF
Unit 10.17 Web-Based Analysis and Publication of Flow Cytometry Experiments
Abstract | Full Text: HTML PDF
Unit 12.20 Approaches to Spectral Imaging Hardware
Abstract | Full Text: HTML PDF
Supplement 52, April 2010
Unit 7.2 Critical Aspects in Analysis of Cellular DNA Content
Abstract | Full Text: HTML PDF
Unit 7.36 Click-iT Proliferation Assay with Improved DNA Histograms
Abstract | Full Text: HTML PDF
Unit 9.32 Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology
Abstract | Full Text: HTML PDF
Unit 9.33 Identification of Endothelial Cells and Progenitor Cell Subsets in Human Peripheral Blood
Abstract | Full Text: HTML PDF
Unit 11.19 Life Cycle Analysis of Unicellular Algae
Abstract | Full Text: HTML PDF
Unit 12.19 3D Deconvolution Microscopy
Abstract | Full Text: HTML PDF
Supplement 51, January 2010
Unit 1.24 Practical Issues in High-Speed Cell Sorting
Abstract | Full Text: HTML PDF
Unit 6.28 Measurement of T Cell Activation After 16-hr In Vitro Stimulation with Concanavalin A
Abstract | Full Text: HTML PDF
Unit 9.29 Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods
Abstract | Full Text: HTML PDF
Unit 9.30 Stem Cell Side Population Analysis and Sorting Using DyeCycle Violet
Abstract | Full Text: HTML PDF
Unit 9.31 Measurement of Phagocytosis and of the Phagosomal Environment in Polymorphonuclear Phagocytes by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 13.11 Use of Flow Cytometric Methods to Quantify Protein-Protein Interactions
Abstract | Full Text: HTML PDF
Supplement 50, October 2009
Unit 1.10 Techniques for Flow Cytometer Alignment
Abstract | Full Text: HTML PDF
Unit 1.23 Pulse Width for Particle Sizing
Abstract | Full Text: HTML PDF
Unit 2.15 Measurement of Molecular Mobility with Fluorescence Correlation Spectroscopy
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Unit 6.27 High-Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin-Embedded Tissues
Abstract | Full Text: HTML PDF
Unit 12.18 Total Internal Reflection Fluorescence (TIRF) Microscopy
Abstract | Full Text: HTML PDF
Supplement 49, July 2009
Unit 1.9 Lasers for Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 1.22 Fundamentals of Acoustic Cytometry
Abstract | Full Text: HTML PDF
Unit 6.26 Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements
Abstract | Full Text: HTML PDF
Unit 11.18 Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single-Cell Level by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 12.17 Simultaneous Optical Mapping of Intracellular Free Calcium and Action Potentials from Langendorff Perfused Hearts
Abstract | Full Text: HTML PDF
Supplement 48, April 2009
Unit 12.15 Cryosectioning
Abstract | Full Text: HTML PDF
Unit 12.16 Immunohistochemistry
Abstract | Full Text: HTML PDF
Unit 13.10 Multiplexed Analysis of Peptide Antigen-Specific Antibodies
Abstract | Full Text: HTML PDF
Supplement 47, January 2009
Unit 1.4 Establishing and Maintaining System Linearity
Abstract | Full Text: HTML PDF
Unit 9.28 Measurement of Cytoplasmic to Nuclear Translocation
Abstract | Full Text: HTML PDF
Supplement 46, October 2008
Unit 2.3 Light Microscopy Digital Imaging
Abstract | Full Text: HTML PDF
Unit 7.35 Assessment of Histone Acetylation Levels in Relation to Cell Cycle Phase
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Unit 9.26 Immunophenotyping and DNA Content Analysis of Acetone-Fixed Cells
Abstract | Full Text: HTML PDF
Unit 9.27 Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 45, July 2008
Unit 4.7 Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries
Abstract | Full Text: HTML PDF
Unit 6.25 Flow Cytometric Immunophenotyping of Cerebrospinal Fluid
Abstract | Full Text: HTML PDF
Unit 7.34 Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining
Abstract | Full Text: HTML PDF
Unit 12.13 Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide-Based Signaling Events in Isolated Perfused Lung
Abstract | Full Text: HTML PDF
Unit 12.14 Flow Cytometric FRET Analysis of ErbB Receptor Tyrosine Kinase Interaction
Abstract | Full Text: HTML PDF
Supplement 44, April 2008
Unit 9.25 Advanced Application of CFSE for Cellular Tracking
Abstract | Full Text: HTML PDF
Unit 12.12 From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism
Abstract | Full Text: HTML PDF
Unit 13.9 Multiplexed Detection of Fungal Nucleic Acid Signatures
Abstract | Full Text: HTML PDF
Supplement 43, January 2008
Unit 2.14 Methods and Applications of Laser-Enabled Analysis and Processing (LEAP)
Abstract | Full Text: HTML PDF
Unit 12.11 Confocal Imaging of Cell Division
Abstract | Full Text: HTML PDF
Appendix 3C Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
Abstract | Full Text: HTML PDF
Appendix 3I Production of Polyclonal Antisera
Abstract | Full Text: HTML PDF
Supplement 42, October 2007
Unit 4.6 Flow Cytometric Sorting of Bacterial Surface-Displayed Libraries
Abstract | Full Text: HTML PDF
Unit 7.33 SYTO Probes: Markers of Apoptotic Cell Demise
Abstract | Full Text: HTML PDF
Unit 10.16 Alternatives to Log-Scale Data Display
Abstract | Full Text: HTML PDF
Unit 12.10 Detecting Protein-Protein Interactions In Vivo with FRET using Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM)
Abstract | Full Text: HTML PDF
Supplement 41, July 2007
Unit 7.32 Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1
Abstract | Full Text: HTML PDF
Unit 9.24 High-Throughput Cytotoxicity Screening by Propidium Iodide Staining
Abstract | Full Text: HTML PDF
Unit 12.9 Live-Animal Imaging of Renal Function by Multiphoton Microscopy
Abstract | Full Text: HTML PDF
Supplement 40, April 2007
Unit 1.20 Characterization of Flow Cytometer Instrument Sensitivity
Abstract | Full Text: HTML PDF
Unit 1.21 Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer
Abstract | Full Text: HTML PDF
Unit 7.31 Assessment of Cell Proliferation by 5-Bromodeoxyuridine (BrdU) Labeling for Multicolor Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 39, January 2007
Unit 1.1 Overview of Flow Cytometry Instrumentation
Abstract | Full Text: HTML PDF
Unit 3.6 Standard Safety Practices for Sorting of Unfixed Cells
Abstract | Full Text: HTML PDF
Unit 9.22 Optimized Whole-Blood Assay for Measurement of ZAP-70 Protein Expression
Abstract | Full Text: HTML PDF
Unit 9.23 Flow Cytometry of the Side Population (SP)
Abstract | Full Text: HTML PDF
Supplement 38, October 2006
Unit 1.12 Principles of Resonance Energy Transfer
Abstract | Full Text: HTML PDF
Unit 2.13 Optimizing Laser Source Operation for Confocal and Multiphoton Laser Scanning Microscopy
Abstract | Full Text: HTML PDF
Unit 7.30 Estimation of Relative Nuclear DNA Content in Dehydrated Plant Tissues by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 12.8 Measuring FRET in Flow Cytometry and Microscopy
Abstract | Full Text: HTML PDF
Supplement 37, July 2006
Unit 10.14 Intensity Calibration and Shading Correction for Fluorescence Microscopes
Abstract | Full Text: HTML PDF
Unit 13.8 Reagents and Instruments for Multiplexed Analysis Using Microparticles
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Appendix 3I Production of Polyclonal Antisera
HTML PDF
Appendix 3J Production of Monoclonal Antibodies
Abstract | Full Text: HTML PDF
Appendix 3K Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Abstract | Full Text: HTML PDF
Supplement 36, April 2006
Unit 3.4 Safe Use of Hazardous Chemicals
Abstract | Full Text: HTML PDF
Unit 6.23 Immunophenotyping of Plasma Cells
Abstract | Full Text: HTML PDF
Unit 6.24 Flow Rate Calibration for Absolute Cell Counting Rationale and Design
Abstract | Full Text: HTML PDF
Appendix 3B Techniques for Mammalian Cell Tissue Culture
Abstract | Full Text: HTML PDF
Supplement 35, January 2006
Unit 7.29 Nuclear DNA Content Analysis of Plant Seeds by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 12.7 Detecting Protein–Protein Interactions with CFP-YFP FRET by Acceptor Photobleaching
Abstract | Full Text: HTML PDF
Unit 13.1 Multiplexed Microsphere-Based Flow Cytometric Immunoassays
Abstract | Full Text: HTML PDF
Unit 13.6 Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
Abstract | Full Text: HTML PDF
Unit 13.7 Detection of Gene Fusions in Acute Leukemia Using Bead Microarrays
Abstract | Full Text: HTML PDF
Supplement 34, October 2005
Unit 13.2 Microsphere Surface Protein Determination Using Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 13.3 Use of Microsphere-Supported Phospholipid Membranes for Analysis of Protein-Lipid Interactions
Abstract | Full Text: HTML PDF
Unit 13.4 Multiplexed SNP Genotyping Using Primer Single-Base Extension (SBE) and Microsphere Arrays
Abstract | Full Text: HTML PDF
Unit 13.5 BeadCons: Detection of Nucleic Acid Sequences by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 33, July 2005
Unit 6.21 Ten-Color Immunophenotyping of Hematopoietic Cells
Abstract | Full Text: HTML PDF
Unit 6.22 Flow Cytometric Screening for the HLA-B27 Antigen on Peripheral Blood Lymphocytes
Abstract | Full Text: HTML PDF
Unit 7.28 RNA and DNA Aptamers in Cytomics Analysis
Abstract | Full Text: HTML PDF
Unit 12.6 Quantitative Fluorescence In Situ Hybridization (QFISH) of Telomere Lengths in Tissue and Cells
Abstract | Full Text: HTML PDF
Supplement 32, April 2005
Unit 1.3 Standardization, Calibration, and Control in Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.19 ZAP-70 Staining in Chronic Lymphocytic Leukemia
Abstract | Full Text: HTML PDF
Unit 6.20 Multiparameter Analysis of Intracellular Phosphoepitopes in Immunophenotyped Cell Populations by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 12.5 Three-Dimensional Visualization of Blood and Lymphatic Vasculature in Tissue Whole Mounts Using Confocal Microscopy
Abstract | Full Text: HTML PDF
Supplement 31, January 2005
Unit 12.1 Comparative Overview of Flow and Image Cytometry
Abstract | Full Text: HTML PDF
Unit 12.2 Basics of Digital Microscopy
Abstract | Full Text: HTML PDF
Unit 12.3 Modern Confocal Microscopy
Abstract | Full Text: HTML PDF
Unit 12.4 Time-Lapse Microscopy Approaches to Track Cell Cycle Progression at the Single-Cell Level
Abstract | Full Text: HTML PDF
Supplement 30, October 2004
Unit 6.3 High-Sensitivity Immunofluorescence/Flow Cytometry: Detection of Cytokine Receptors and Other Low-Abundance Membrane Molecules
Abstract | Full Text: HTML PDF
Unit 6.18 Identification of Human Antigen-Specific T Cells Using MHC Class I and Class II Tetramers
Abstract | Full Text: HTML PDF
Unit 7.3 Differential Staining of DNA and RNA
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Unit 7.27 Detection of Histone H2AX Phosphorylation on Ser-139 as an Indicator of DNA Damage (DNA Double-Strand Breaks)
Abstract | Full Text: HTML PDF
Unit 11.1 Overview of Flow Cytometry and Microbiology
Abstract | Full Text: HTML PDF
Supplement 29, July 2004
Unit 7.26 Assessment of Telomere Length, Phenotype, and DNA Content
Abstract | Full Text: HTML PDF
Unit 11.3 Estimation of Microbial Viability Using Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 11.17 Labeling of Bacterial Pathogens for Flow Cytometric Detection and Enumeration
Abstract | Full Text: HTML PDF
Supplement 28, April 2004
Unit 2.10 Scanning Laser Cytometry
Abstract | Full Text: HTML PDF
Unit 6.17 Enumeration of Fetal Red Blood Cells, F Cells, and F Reticulocytes in Human Blood
Abstract | Full Text: HTML PDF
Unit 7.24 Detection of Mitotic Cells
Abstract | Full Text: HTML PDF
Unit 7.25 DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells
Abstract | Full Text: HTML PDF
Unit 9.6 Estimation of Membrane Potential by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 9.9 Intracellular Cytokines
Abstract | Full Text: HTML PDF
Supplement 27, January 2004
Unit 1.9 Lasers for Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.16 Flow Cytometric Assessment of HLA Alloantibodies
Abstract | Full Text: HTML PDF
Unit 8.11 Principles and Applications of PRINS in Cytogenetics
Abstract | Full Text: HTML PDF
Unit 9.11 Flow Cytometric Analysis of Cell Division by Dye Dilution
Abstract | Full Text: HTML PDF
Unit 11.2 Flow Cytometry and Environmental Microbiology
Abstract | Full Text: HTML PDF
Unit 11.7 Mycobacterium tuberculosis Susceptibility Testing by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 26, October 2003
Unit 1.19 Excitation and Emission Spectra of Common Dyes
Abstract | Full Text: HTML PDF
Unit 4.3 Nucleic Acid Probes
Abstract | Full Text: HTML PDF
Unit 7.22 Analysis of Tissue Imprints by Scanning Laser Cytometry
Abstract | Full Text: HTML PDF
Unit 7.23 Cell Cycle Analysis of Budding Yeast Using SYTOX Green
Abstract | Full Text: HTML PDF
Unit 8.13 Combined Immunofluorescence and FISH: New Prospects for Tumor Cell Detection/Identification
Abstract | Full Text: HTML PDF
Unit 11.5 Detection of Borreliacidal Antibodies by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 25, July 2003
Unit 6.4 Enumeration of CD34 Hematopoietic Stem and Progenitor Cells
Abstract | Full Text: HTML PDF
Unit 7.19 Flow Cytometry of Apoptosis
Abstract | Full Text: HTML PDF
Unit 9.21 Flow Cytometric Analysis of Cytokine Responses in Stimulated Whole Blood: Simultaneous Quantitation of TNF-α-Secreting Cells and Soluble Cytokines
Abstract | Full Text: HTML PDF
Supplement 24, April 2003
Unit 6.15 Whole Blood Analysis of Leukocyte-Platelet Aggregates
Abstract | Full Text: HTML PDF
Unit 9.20 Flow Cytometric Analysis of Calcium Mobilization in Whole-Blood Platelets
Abstract | Full Text: HTML PDF
Unit 11.16 Functional Assays of Oxidative Stress Using Genetically Engineered Escherichia coli Strains
Abstract | Full Text: HTML PDF
Supplement 23, January 2003
Unit 6.13 Immunophenotyping Using a Laser Scanning Cytometer
Abstract | Full Text: HTML PDF
Unit 6.14 Enzymatic Amplification Staining for Cell Surface Antigens
Abstract | Full Text: HTML PDF
Unit 7.21 Measurement of Cytogenetic Damage in Rodent Blood with a Single-Laser Flow Cytometer
Abstract | Full Text: HTML PDF
Unit 10.15 A Software Method for Color Compensation
Abstract | Full Text: HTML PDF
Unit 11.15 Resolution of Viable and Membrane-Compromised Free Bacteria in Aquatic Environments by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 22, October 2002
Unit 1.14 Compensation in Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.12 Quantitative Flow Cytometric Analysis of Membrane Antigen Expression
Abstract | Full Text: HTML PDF
Unit 7.19 Flow Cytometry of Apoptosis
Unit 7.20 Analysis of Fine-Needle Aspirate Biopsies from Solid Tumors by Laser Scanning Cytometry (LSC)
Abstract | Full Text: HTML PDF
Supplement 21, July 2002
Unit 7.18 Apoptosis Signaling Pathways
Abstract | Full Text: HTML PDF
Unit 9.19 Assessment of Phagocyte Functions by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 10.14 Intensity Calibration and Shading Correction for Fluorescence Microscopes
HTML PDF
Supplement 20, April 2002
Unit 1.18 Dynamic Thermoregulation of the Sample in Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 3.4 Safe Use of Hazardous Chemicals
HTML PDF
Unit 6.11 Immunophenotypic Analysis of PNH Cells
Abstract | Full Text: HTML PDF
Unit 10.13 The Application of Data Mining to Flow Cytometry
Abstract | Full Text: HTML PDF
Appendix 1A Abbreviations Used in this Manual
Abstract | Full Text: HTML PDF
Supplement 19, January 2002
Unit 3.5 Method for Visualizing Aerosol Contamination in Flow Sorters
Abstract | Full Text: HTML PDF
Unit 6.10 Immunophenotypic Analysis of Platelets
Abstract | Full Text: HTML PDF
Unit 9.18 Stem Cell Identification and Sorting Using the Hoechst 33342 Side Population (SP)
Abstract | Full Text: HTML PDF
Unit 10.12 Dial-In Flow Cytometry Data Analysis
Abstract | Full Text: HTML PDF
Supplement 18, October 2001
Unit 3.4 Safe Use of Hazardous Chemicals
Unit 8.11 Principles and Applications of PRINS in Cytogenetics
Abstract | Full Text: HTML PDF
Unit 8.12 Comparative Genomic Hybridization (CGH)—Detection of Unbalanced Genetic Aberrations Using Conventional and Micro-Array Techniques
Abstract | Full Text: HTML PDF
Supplement 17, July 2001
Unit 1.17 Plug Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.9 Immunophenotypic Identification, Enumeration, and Characterization of Human Peripheral Blood Dendritic Cells and Dendritic-Cell Precursors
Abstract | Full Text: HTML PDF
Unit 7.17 Analysis of Viral Infection and Viral and Cellular DNA and Proteins by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 9.17 Assessment of Surface Markers and Functionality of Dendritic Cells (DCs)
Abstract | Full Text: HTML PDF
Supplement 16, April 2001
Unit 1.16 Simultaneous Analysis of the Cyan, Green, and Yellow Fluorescent Proteins
Abstract | Full Text: HTML PDF
Unit 7.15 Ultraviolet-Induced Detection of Halogenated Pyrimidines (UVID)
Abstract | Full Text: HTML PDF
Unit 7.16 Analysis of DNA Content and Green Fluorescent Protein Expression
Abstract | Full Text: HTML PDF
Unit 8.8 Single-Nucleotide Sequence Discrimination In Situ Using Padlock Probes
Abstract | Full Text: HTML PDF
Unit 8.10 Molecular Combing
Abstract | Full Text: HTML PDF
Supplement 15, January 2001
Unit 2.4 Optical Filtering Systems for Wavelength Selection in Light Microscopy
Abstract | Full Text: HTML PDF
Unit 2.12 Photobleaching Measurements of Diffusion in Cell Membranes and Aqueous Cell Compartments
Abstract | Full Text: HTML PDF
Unit 7.14 Analysis of Cell Proliferation and Cell Survival by Continuous BrdU Labeling and Multivariate Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 8.11 Principles and Applications of PRINS in Cytogenetics
Unit 11.14 Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes
Abstract | Full Text: HTML PDF
Appendix 1B Common Conversion Factors
Abstract | Full Text: HTML PDF
Appendix 3H Importing Biological Materials
Abstract | Full Text: HTML PDF
Supplement 14, October 2000
Unit 2.11 Shading Correction: Compensation for Illumination and Sensor Inhomogeneities
Abstract | Full Text: HTML PDF
Unit 4.5 Spectroscopic Analysis Using DNA and RNA Fluorescent Probes
Abstract | Full Text: HTML PDF
Unit 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy
Abstract | Full Text: HTML PDF
Unit 9.15 Multiparameter Analysis of Physiological Changes in Apoptosis
Abstract | Full Text: HTML PDF
Unit 9.16 Signal Transduction During Natural Killer Cell Activation
Abstract | Full Text: HTML PDF
Supplement 13, July 2000
Unit 6.8 Enumeration of Absolute Cell Counts Using Immunophenotypic Techniques
Abstract | Full Text: HTML PDF
Unit 7.13 Sperm Chromatin Structure Assay for Fertility Assessment
Abstract | Full Text: HTML PDF
Unit 9.14 Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1
Abstract | Full Text: HTML PDF
Unit 10.11 Image Processing and 2-D Morphometry
Abstract | Full Text: HTML PDF
Unit 11.13 Cell Cycle Analysis of Yeasts
Abstract | Full Text: HTML PDF
Supplement 12, April 2000
Unit 6.6 Immunophenotypic Analysis of Human Mast Cells by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 6.7 Measurement of CD40 Ligand (CD154) Expression on Resting and In Vitro–Activated T Cells
Abstract | Full Text: HTML PDF
Unit 7.12 Flow Cytometric Analysis of RNA Synthesis by Detection of Bromouridine Incorporation
Abstract | Full Text: HTML PDF
Unit 9.13 In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix
Abstract | Full Text: HTML PDF
Appendix 3G Growing Bacteria on Solid Media
Abstract | Full Text: HTML PDF
Supplement 11, January 2000
Unit 1.15 Time-Resolved Fluorescence Measurements
Abstract | Full Text: HTML PDF
Unit 6.5 Immunophenotypic Analysis of Peripheral Blood Lymphocytes
Abstract | Full Text: HTML PDF
Unit 8.9 Tyramide Signal Amplification (TSA) Systems for the Enhancement of ISH Signals in Cytogenetics
Abstract | Full Text: HTML PDF
Unit 11.12 DNA/RNA Analysis of Phytoplankton by Flow Cytometry
Abstract | Full Text: HTML PDF
Appendix 3C Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
HTML PDF
Appendix 3D Wright-Giemsa and Nonspecific Esterase Staining of Cells
Abstract | Full Text: HTML PDF
Appendix 3E Techniques for Bacterial Cell Culture: Media Preparation and Bacteriological Tools
Abstract | Full Text: HTML PDF
Appendix 3F Growing Bacteria in Liquid Media
Abstract | Full Text: HTML PDF
Supplement 10, October 1999
Unit 1.14 Compensation in Flow Cytometry
Unit 2.10 Scanning Laser Cytometry
Unit 7.11 Assessment of Viability, Immunofluorescence, and DNA Content
Abstract | Full Text: HTML PDF
Unit 10.10 Three-Dimensional Image Visualization and Analysis
Abstract | Full Text: HTML PDF
Unit 11.11 Enumeration of Phytoplankton, Bacteria, and Viruses in Marine Samples
Abstract | Full Text: HTML PDF
Supplement 9, July 1999
Unit 1.12 Principles of Resonance Energy Transfer
HTML PDF
Unit 1.13 Light Scatter: Detection and Usage
Abstract | Full Text: HTML PDF
Unit 5.3 Flow Analysis and Sorting of Plant Chromosomes
Abstract | Full Text: HTML PDF
Unit 8.8 Single-Nucleotide Sequence Discrimination In Situ Using Padlock Probes
Unit 11.9 Determination of Bacterial Biomass from Flow Cytometric Measurements of Forward Light Scatter Intensity
Abstract | Full Text: HTML PDF
Unit 11.10 Flow Cytometry of Yeasts
Abstract | Full Text: HTML PDF
Supplement 8, April 1999
Unit 6.4 Enumeration of CD34 Hematopoietic Stem and Progenitor Cells
Unit 11.6 Flow Cytometric Detection of Pathogenic E. coli in Food
Abstract | Full Text: HTML PDF
Unit 11.7 Mycobacterium tuberculosis Susceptibility Testing by Flow Cytometry
Unit 11.8 Antibiotic Susceptibility Testing by Flow Cytometry
Abstract | Full Text: HTML PDF
Supplement 7, January 1999
Unit 1.11 Flow Cytometers for Characterization of Microorganisms
Abstract | Full Text: HTML PDF
Unit 9.12 Reporters of Gene Expression: Autofluorescent Proteins
Abstract | Full Text: HTML PDF
Unit 11.1 Overview of Flow Cytometry and Microbiology
HTML PDF
Unit 11.2 Flow Cytometry and Environmental Microbiology
Unit 11.3 Estimation of Microbial Viability Using Flow Cytometry
HTML PDF
Unit 11.4 Sorting of Bacteria
Abstract | Full Text: HTML PDF
Unit 11.5 Detection of Borreliacidal Antibodies by Flow Cytometry
Supplement 6, October 1998
Unit 1.10 Techniques for Flow Cytometer Alignment
Full Text: HTML PDF
Unit 2.9 Multiphoton Imaging
Abstract | Full Text: HTML PDF
Unit 7.10 Flow Cytometric Analysis of Reticulated Platelets
Abstract | Full Text: HTML PDF
Unit 8.7 Combination DNA/RNA Fish and Immunophenotyping
Abstract | Full Text: HTML PDF
Unit 9.5 Reporters of Gene Expression: Enzymatic Assays
Abstract | Full Text: HTML PDF
Unit 9.11 Flow Cytometric Analysis of Cell Division by Dye Dilution
Supplement 5, July 1998
Unit 2.3 Cameras
Abstract | Full Text: HTML PDF
Unit 2.8 Confocal Microscopy: Principles and Practices
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Unit 6.3 High-Sensitivity Immunofluorescence/Flow Cytometry: Detection of Cytokine Receptors and Other Low-Abundance Membrane Molecules
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Unit 8.5 Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics
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Unit 8.6 Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics
Abstract | Full Text: HTML PDF
Supplement 4, April 1998
Unit 1.9 Lasers for Flow Cytometry
Unit 2.6 Calibration: Sampling Density and Spatial Resolution
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Unit 7.9 Bivariate Analysis of DNA Content and Expression of Cyclin Proteins
Abstract | Full Text: HTML PDF
Unit 8.4 Immunocytochemical Detection
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Unit 9.10 Assays of Natural Killer (NK) Cell Ligation to Target Cells
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Supplement 3, January 1998
Unit 1.8 Principles of Gating
Abstract | Full Text: HTML PDF
Unit 7.8 Analysis of DNA Denaturation
Abstract | Full Text: HTML PDF
Unit 9.9 Intracellular Cytokines
Unit 10.8 Multivariate Analysis
Abstract | Full Text: HTML PDF
Unit 10.9 Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry
Abstract | Full Text: HTML PDF
Supplement 2, October 1997
Unit 2.7 Microscope Alignment
Abstract | Full Text: HTML PDF
Unit 4.4 Cellular Function Probes
Abstract | Full Text: HTML PDF
Unit 7.6 Analysis of Nuclear DNA Content and Ploidy in Higher Plants
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Unit 7.7 Analysis of DNA Content and BrdU Incorporation
Abstract | Full Text: HTML PDF
Unit 9.7 Oxidative Metabolism
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Unit 9.8 Measurement of Intracellular Calcium Ions by Flow Cytometry
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Supplement 1, July 1997
Unit 1.2 Fluidics
Abstract | Full Text: HTML PDF
Unit 1.6 Laser Beam Shaping and Spot Size
Abstract | Full Text: HTML PDF
Unit 1.7 High-Speed Cell Sorting
Abstract | Full Text: HTML PDF
Unit 2.6 Calibration: Sampling Density and Spatial Resolution
Unit 3.3 Testing the Efficiency of Aerosol Containment During Cell Sorting
Abstract | Full Text: HTML PDF
Unit 9.6 Estimation of Membrane Potential by Flow Cytometry
Unit 10.6 Data Presentation
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Unit 10.7 Data Analysis Through Modeling
Abstract | Full Text: HTML PDF
Core Publication, April 1997
Unit 1.1 Overview of Flow Cytometry Instrumentation
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Unit 1.3 Standardization, Calibration, and Control in Flow Cytometry
HTML PDF
Unit 1.4 Establishing and Maintaining System Linearity
HTML PDF
Unit 1.5 Optical Filter Sets for Multiparameter Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 2.1 Contrast Enhancement in Light Microscopy
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Unit 2.2 Microscope Objectives
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Unit 2.3 Cameras
Unit 2.4 Optical Filtering Systems for Wavelength Selection in Light Microscopy
Unit 2.5 Digital Fluorescence Microscopy
Abstract | Full Text: HTML PDF
Unit 3.1 Principles of Quality Control
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Unit 3.2 Components of Quality Control
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Unit 4.1 Titering Antibodies
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Unit 4.2 Conjugation of Fluorochromes to Monoclonal Antibodies
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Unit 4.3 Nucleic Acid Probes
Unit 5.1 Handling, Storage, and Preparation of Human Blood Cells
Abstract | Full Text: HTML PDF
Unit 5.2 Handling, Storage, and Preparation of Human Tissues
Abstract | Full Text: HTML PDF
Unit 6.1 Quality Control in Phenotypic Analysis by Flow Cytometry
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Unit 6.2 Immunophenotyping
Abstract | Full Text: HTML PDF
Unit 7.1 Overview of Nucleic Acid Analysis
Abstract | Full Text: HTML PDF
Unit 7.2 Quality Control in Nucleic Acid Analysis
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Unit 7.3 Differential Staining of DNA and RNA
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Unit 7.4 Analysis of DNA Content and DNA Strand Breaks for Detection of Apoptotic Cells
Abstract | Full Text: HTML PDF
Unit 7.5 DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis
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Unit 8.1 Overview of Fluorescence In Situ Hybridization Techniques for Molecular Cytogenetics
Abstract | Full Text: HTML PDF
Unit 8.2 Basic Preparative Techniques for Fluorescence In Situ Hybridization
Abstract | Full Text: HTML PDF
Unit 8.3 Probe Labeling and Fluorescence In Situ Hybridization
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Unit 9.1 Overview of Functional Cell Assays
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Unit 9.2 Assessment of Cell Viability
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Unit 9.3 Flow Cytometric Measurement of Intracellular pH
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Unit 9.4 Analysis of Intracellular Organelles by Flow Cytometry or Microscopy
Unit 9.5 Reporters of Gene Expression: Enzymatic Assays
Unit 10.1 Data Management
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Unit 10.2 Data File Standard for Flow Cytometry, FCS 3.0
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Unit 10.3 Listmode Data Processing
Abstract | Full Text: HTML PDF
Unit 10.4 Multidimensional Data Analysis in Immunophenotyping
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Unit 10.5 Two-Dimensional Image Processing and Analysis
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Appendix 1A Abbreviations Used in this Manual
Appendix 1B Common Conversion Factors
Appendix 2A Common Stock Solutions, Buffers, and Media
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Appendix 3A Cell Counting
Abstract | Full Text: HTML PDF
Appendix 3B Techniques for Mammalian Cell Tissue Culture
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