Current Protocols in Cytometry
Online ISBN: 9780471142959
Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.
Current Protocols in Cytometry is the comprehensive resource for all types of protocols in cytomics and imaging. The expansion of cell-based analysis systems across all fields of biology has been driven by the demand for high-content information integrated across proteomics and genomics. Cytometry covers a wide array of technologies, all of which are complex and demand attention to assay design, data collection, and multivariate analysis. Current Protocols in Cytometry features step-by-step instructions for working with hundreds of complex assays utilizing tracking probes, multiple reporter molecules, and frequently huge population statistics. There are basic science methods as well as clinical assays for application in clinical diagnostics using a large number of reagents certified for diagnostic use. Vital information such as safety issues for highly dangerous pathogens is covered in detail. The publication presents approaches ranging from single-cell analysis using flow cytometry to large-scale imaging using high-content screening and analysis. An extensive section of the publication covers advanced data analysis integrating current techniques of classification and automated identification. With quarterly updates, Current Protocols in Cytometry continues to add new and updated protocols to the content.
The target audience for Current Protocols in Cytometry is anyone using cellular assays systems for the purposes of studying cellular pathways, impact of drugs on signaling cascades, clinical applications for diagnostics, microbiology, live-cell imaging, and other cellular analysis. Protocols are written in sufficient detail so that readers can easily follow and perform the analyses described on their own, making Current Protocols in Cytometry the ideal companion for experimentalists in all biological disciplines.
cell biologists • immunologists • molecular biologists • microbiologists • hematologists • medical technologists • clinical pathologists • biotechnologists
cytometry, cytomics, monoclonal antibodies, fluorescence, flow cytometry, biological imaging, high-content screening, high-content analysis, laser scanning cytometry, population statistics, biotechnology, genes, computational chemistry, laboratory protocols, Current Protocols