Current Protocols in Cytometry

Current Protocols in Cytometry

Online ISBN: 9780471142959

DOI: 10.1002/0471142956

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
    1. Adhesion
    2. Analysis of Proteins
    3. Antibodies as Tools
    4. Apoptosis
    5. Cancer
    6. Cell and Organelle Motility
    7. Cell Cycle Analysis
    8. Cell Isolation and Culture
    9. Enzyme Assays
    10. Extracellular Matrix
    11. Fluorescent Proteins as Probes of Structure and Function
    12. Histology
    13. Labeling Molecules
    14. Microscopy and Imaging
      1. 3D Deconvolution Microscopy
      2. A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non-Vesicular Organelles
      3. A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm
      4. Uncompensated Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1 and Multilaser Excitation
      5. Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching (FRAP)
      6. Characterization of Surface FAS—Quantitative Morphological Analysis Using Quantitative Imaging Cytometry
      7. Application of Flow-FISH for Dynamic Measurement of Telomere Length in Cell Division
      8. A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures
      9. Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture
      10. Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining
      11. Confocal Microscopy for High-Resolution and High-Content Analysis of the Cell Cycle
      12. Evaluation and Purchase of Confocal Microscopes: Numerous Factors to Consider
      13. Correlative Fluorescence and Electron Microscopy
      14. Cryosectioning
      15. Ex Vivo Imaging of Excised Tissue Using Vital Dyes and Confocal Microscopy
      16. Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes
      17. Measurement of Intracellular Ions by Flow Cytometry
      18. Flow Cytometry of Murine Spermatocytes
      19. Flow Cytometry of the Side Population (SP)
      20. Flow Cytometry–Based Cytotoxicity and Antibody Binding Assay
      21. Fluidics
      22. Fountain Flow Cytometry
      23. FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells
      24. High-Resolution Cytometry for High-Content Cell Cycle Analysis
      25. How to Build a Time-Gated Luminescence Microscope
      26. Imaging Autophagy
      27. In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
      28. In Vivo Immuno-Spin Trapping: Imaging the Footprints of Oxidative Stress
      29. Detection of Intracellular Glutathione Using ThiolTracker Violet Stain and Fluorescence Microscopy
      30. Light Sheet Fluorescence Microscopy (LSFM)
      31. Live Imaging of the Lung
      32. Live-Animal Imaging of Renal Function by Multiphoton Microscopy
      33. Microscopic Investigation of Protein Function in C. elegans Using Fluorescent Imaging
      34. Multi-Photon Imaging
      35. Near-Infrared Molecular Probes for In Vivo Imaging
      36. Practical Methods for Molecular In Vivo Optical Imaging
      37. Quantitative Fluorescent Speckle Microscopy (QFSM) to Measure Actin Dynamics
      38. Semi-Automated Object Tracking Methods in Biological Imaging
      39. Setting Up and Running an Advanced Light Microscopy and Imaging Facility
      40. Super-Resolution Microscopy: A Comparative Treatment
      41. Building a Live Cell Microscope: What You Need and How to Do It
      42. The Application of KillerRed for Acute Protein Inactivation in Living Cells
      43. Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues
      44. Total Internal Reflection Fluorescence (TIRF) Microscopy
      45. Total Internal Reflection Fluorescence (TIRF) Microscopy Illuminator for Improved Imaging of Cell Surface Events
      46. Two-Photon Imaging of the Immune System
      47. Visualization of Telomere Integrity and Function In Vitro and In Vivo Using Immunofluorescence Techniques
    15. Molecular Interactions
    16. Molecular Trafficking
    17. Nuclei and Chromosomes
    18. Organelle Analysis
    19. Plant Biology
    20. Signal Transduction
    21. Stem Cells and Differentiation
    22. Subcellular Fractionation
    23. Viruses
    24. Whole Organism and Tissue Analysis
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Cytometry
  8. Gene Expression
  9. Genetics and Genomics
  10. Imaging and Microscopy
  11. Imaging/Microscopy
  12. Immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Microbiology
  16. Molecular Biology
  17. Mouse Biology
  18. Mouse Biology
  19. Neuroscience
  20. Neuroscience
  21. Nucleic Acid Chemistry
  22. Pharmacology and Drug Discovery
  23. Protein Production, Purification, and Analysis
  24. Proteomics
  25. RNA
  26. Stem Cells
  27. Supporting Lab Techniques
  28. Toxicology

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